M.Sc. Huseyn Huseynli
The display and reactivity of autoimmunity-relevant T cell antigens bound to HLA-DQ molecules
Protein Biochemistry
PhD Student
Room 025/027
14195 Berlin
HLA-DQ belongs to the MHC class II family, which is expressed by the professional antigen-presenting cells as part of the adaptive immune response. MHC class II molecules are encoded by one of the most polymorphic genes in the human genome, and individuals can express multiple HLA-DQ heterodimers, influencing their susceptibility to different pathological processes. This project focuses on the HLA-DQ2.5, DQ8.1, and DQ6.2 allotypes, first two of which have been strongly implicated in the pathogenesis of Type 1 Diabetes (T1D) and Celiac Disease (CD), whereas the DQ6.2 allotype is postulated to confer protection under these conditions.
Huseyn Huseynli holds a M.Sc. in Molecular Biotechnology from Anhalt University of Applied Sciences. He conducted his master thesis on Meprin ß which is postulated to be involved in the pathogenesis of Alzheimer’s disease. He used the wild type and its three variants, each having a single amino acid exchange in its active site. During his thesis period, he developed the expression system and the methodology, expressed wild type and three mutated variants of the meprin ß using methylotrophic Pichia pastoris, carried out kinetic measurements (Km, Ki, IC50 etc.) using two synthetic substrates, and three in-house developed inhibitors, to find out the amino acid with utmost importance to substrate and inhibitor binding.
Keywords
- HLA-DQ2.5, 8.1, 6.2 disease association, T1D HLA-DQ, CD HLA-DQ