Alexandra Mitlehner
Antigenpresentation on MHC class I proteins
Biochemistry
PhD Student
Protein Biochemistry
14195 Berlin
In her PhD research Alexandra Mitlehner investigates how antigenic peptides are loaded onto MHC class I proteins (MHC-I), which are then presented on the surface of every nucleated cell. To elucidate how the protein tapasin facilitates peptide exchange she purifies and analyzes different constructs which are then used for cryogenic electron microscopy to study the structure of the protein complex. Furthermore, she investigates the influence of small molecules on MHC-I proteins using NMR spectroscopy.
Alexandra Mitlehner studied Biochemistry in Greifswald (bachelor and master) during her time in the research group for biophysical chemistry where she also worked as an assistant, she developed and optimized protein purification to use the proteins for CD spectroscopy, cell studies or interaction analysis (using SPR, ITC, etc.). Thereafter, she started her PhD at the group of Christian Freund in 2023.
Keywords
- Protein-protein interactions, NMR, structural studies