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Understanding the elusive protein corona of thermoresponsive nanogels

Miceli, Enrico and Kuropka, Benno and Rosenauer, Christine and Osorio Blanco, Ernesto R and Theune, Loryn E and Kar, Mrityunjoy and Weise, Christoph and Morsbach, Svenja and Freund, Christian and Calderón, Marcelo – 2018

Aim: We analyzed the protein corona of thermoresponsive, poly(N-isopropylacrylamide)- or poly(N-isopropylmethacrylamide)-based nanogels. Materials & methods: Traces of protein corona detected after incubation in human serum were characterized by proteomics and dynamic light scattering in undiluted serum. Results: Apolipoprotein B-100 and albumin were the main components of the protein coronae. For dendritic polyglycerol-poly(N-isopropylacrylamide) nanogels at 37�C, an increase in adsorbed immunoglobulin light chains was detected, followed by partially reversible nanogel aggregation. All nanogels in their hydrophilic state are colloidally stable in serum and bear a dysopsonin-rich protein corona. Conclusion: We observed strong changes in NG stability upon slight alterations in the composition of the protein coronae according to nanogel solvation state. Nanogels in their hydrophilic state possess safe protein coronae.

Title
Understanding_Miceli
Author
Miceli, Enrico and Kuropka, Benno and Rosenauer, Christine and Osorio Blanco, Ernesto R and Theune, Loryn E and Kar, Mrityunjoy and Weise, Christoph and Morsbach, Svenja and Freund, Christian and Calderón, Marcelo
Date
2018-10-18
Identifier
10.2217/nnm-2018-0217
Source(s)
Language
eng
Type
Text