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Publications

2024

  •  Bertazzon M, Hurtado-Pico A, Plaza-Sirvent C, Schuster M, Preußner M, Kuropka B, Liu F, Kirsten AZA, Schmitt XJ, König B, Álvaro-Benito M, Abualrous ET, Albert GI, Kliche S, Heyd F, Schmitz I, Freund C. The nuclear GYF protein CD2BP2/U5-52K is required for T cell homeostasis. Front Immunol. 2024 Sep 6;15:1415839. doi: 10.3389/fimmu.2024.1415839. eCollection 2024. PMID: 39308865.
  • Stockhammer A, Spalt C, Klemt A, Benz LS, Harel S, Natalia V, Wiench L, Freund C, Kuropka B, Bottanelli F. When less is more - A fast TurboID KI approach for high sensitivity endogenous interactome mapping. J Cell Sci. 2024 Jul 26:jcs.261952. doi: 10.1242/jcs.261952. Epub ahead of print. PMID: 39056144.
  • André T, van Berkel AA, Singh G, Abualrous ET, Diwan GD, Schmenger T, Braun L, Malsam J, Toonen RF, Freund C, Russell RB, Verhage M, Söllner TH. Reduced Protein Stability of 11 Pathogenic Missense STXBP1/MUNC18-1 Variants and Improved Disease Prediction. Biol Psychiatry. 2024 Jul 15;96(2):125-136. doi: 10.1016/j.biopsych.2024.03.007. Epub 2024 Mar 13. PMID: 38490366.
  • Metz-Zumaran C, Uckeley ZM, Doldan P, Muraca F, Keser Y, Lukas P, Kuropka B, Küchenhoff L, Rastgou Talemi S, Höfer T, Freund C, Cavalcanti-Adam EA, Graw F, Stanifer M, Boulant S. The population context is a driver of the heterogeneous response of epithelial cells to interferons. Mol Syst Biol. 2024 Mar;20(3):242-275. doi: 10.1038/s44320-024-00011-2. Epub 2024 Jan 25. PMID: 38273161; PMCID: PMC10912784.
  • Zimmermann C, Watson GM, Bauersfeld L, Berry R, Ciblis B, Lan H, Gerke C, Oberhardt V, Fuchs J, Hofmann M, Freund C, Rossjohn J, Momburg F, Hengel H, Halenius A. Diverse cytomegalovirus US11 antagonism and MHC-A evasion strategies reveal a tit-for-tat coevolutionary arms race in hominids. Proc Natl Acad Sci U S A. 2024 Feb 27;121(9):e2315985121. doi: 10.1073/pnas.2315985121. Epub 2024 Feb 20. PMID: 38377192; PMCID: PMC10907249.
  • Labus J, Tang K, Henklein P, Krüger U, Hofmann A, Hondke S, Wöltje K, Freund C, Lucka L, Danker K. The α1 integrin cytoplasmic tail interacts with phosphoinositides and interferes with Akt activation. Biochim Biophys Acta Biomembr. 2024 Feb;1866(2):184257. doi: 10.1016/j.bbamem.2023.184257. Epub 2023 Nov 20. PMID: 37992949.
  • Lolicato F, Steringer JP, Saleppico R, Beyer D, Fernandez-Sobaberas J, Unger S, Klein S, Riegerová P, Wegehingel S, Müller HM, Schmitt XJ, Kaptan S, Freund C, Hof M, Šachl R, Chlanda P, Vattulainen I, Nickel W. Disulfide bridge- dependent dimerization triggers FGF2 membrane translocation into the extracellular space. Elife. 2024 Jan 22;12:RP88579. doi: 10.7554/eLife.88579. PMID: 38252473; PMCID: PMC10945597.

2023

  • Abualrous ET, Stolzenberg S, Sticht J, Wieczorek M, Roske Y, Günther M, Dähn S, Boesen BB, Calvo MM, Biese C, Kuppler F, Medina-García Á, Álvaro-Benito M, Höfer T, Noé F, Freund C. MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange. Nat Chem Biol. 2023 Oct;19(10):1196-1204. doi: 10.1038/s41589-023-01316-3. Epub 2023 May 4. PMID: 37142807; PMCID: PMC10522485.
  • Lan BH, Becker M, Freund C. The mode of action of tapasin on major histocompatibility class I (MHC-I) molecules. J Biol Chem. 2023 Apr;299(4):102987. doi: 10.1016/j.jbc.2023.102987. Epub 2023 Feb 8. PMID: 36758805; PMCID: PMC10040737.

2022

  • Bergfort A, Hilal T, Kuropka B, Ilik İA, Weber G, Aktaş T, Freund C, Wahl MC. The intrinsically disordered TSSC4 protein acts as a helicase inhibitor, placeholder and multi-interaction coordinator during snRNP assembly and recycling. Nucleic Acids Res. 2022 Mar 21;50(5):2938-2958. doi: 10.1093/nar/gkac087.
  • Bergfort A, Preußner M, Kuropka B, Ilik İA, Hilal T, Weber G, Freund C, Aktaş T, Heyd F, Wahl MC. A multi-factor trafficking site on the spliceosome remodeling enzyme BRR2 recruits C9ORF78 to regulate alternative splicing. Nat Commun. 2022 Mar 3;13(1):1132. doi: 10.1038/s41467-022-28754-2.
  • Schmerl B, Gimber N, Kuropka B, Stumpf A, Rentsch J, Kunde SA, von Sivers J, Ewers H, Schmitz D, Freund C, Schmoranzer J, Rademacher N, Shoichet SA. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biol. 2022 Mar 21;20(3): e3001503. doi: 10.1371/journal.pbio.3001503. eCollection 2022 Mar. PMID: 35312684
  • Wenz MT, Bertazzon M, Sticht J, Aleksić S, Gjorgjevikj D, Freund C, Keller BG. Target Recognition in Tandem WW Domains: Complex Structures for Parallel and Antiparallel Ligand Orientation in h-FBP21 Tandem WW. J Chem Inf Model. 2022 Mar 29. doi: 10.1021/acs.jcim.1c01426.
  • Bierlmeier J, Álvaro-Benito M, Scheffler M, Sturm K, Rehkopf L, Freund C, Schwarzer D. Sortase-Mediated Multi-Fragment Assemblies by Ligation Site Switching. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202109032. doi: 10.1002/anie.202109032. Epub 2021 Dec 13.

2021

  • Reisbeck F, Wedepohl S, Dimde M, Schmitt AC, Dernedde J, Álvaro-Benito M, Freund C, Haag R. Synthesis and functionalization of dendritic polyglycerol-based nanogels: application in T cell activation. J Mater Chem B. 2021 Dec 22;10(1):96-106. doi: 10.1039/d1tb02144c.
  • Bräunlein E, Lupoli G, Füchsl F, Abualrous ET, de Andrade Krätzig N, Gosmann D, Wietbrock L, Lange S, Engleitner T, Lan H, Audehm S, Effenberger M, Boxberg M, Steiger K, Chang Y, Yu K, Atay C, Bassermann F, Weichert W, Busch DH, Rad R, Freund C, Antes I, Krackhardt AM. Functional analysis of peripheral and intratumoral neoantigen-specific TCRs identified in a patient with melanoma. J Immunother Cancer. 2021 Sep;9(9):e002754. doi: 10.1136/jitc-2021-002754.
  • Dadwal N, Mix C, Reinhold A, Witte A, Freund C, Schraven B, Kliche S. The Multiple Roles of the Cytosolic Adapter Proteins ADAP, SKAP1 and SKAP2 for TCR/CD3 -Mediated Signaling Events. Front Immunol. 2021 Jul 6;12:703534. doi: 10.3389/fimmu.2021.703534. eCollection 2021.
  • Lan H, Abualrous ET, Sticht J, Fernandez LMA, Werk T, Weise C, Ballaschk M, Schmieder P, Loll B, Freund C. Exchange catalysis by tapasin exploits conserved and allele-specific features of MHC-I molecules. Nat Commun. 2021 Jul 9;12(1):4236. doi: 10.1038/s41467-021-24401-4.
  • Abualrous ET, Sticht J, Freund C. Major histocompatibility complex (MHC) class I and class II proteins: impact of polymorphism on antigen presentation. Curr Opin Immunol. 2021 Jun;70:95-104. doi: 10.1016/j.coi.2021.04.009. Epub 2021 May 27.
  • Gadalla MR, Morrison E, Serebryakova MV, Han X, Wolff T, Freund C, Kordyukova L, Veit M. NS1-mediated upregulation of ZDHHC22 acyltransferase in influenza a virus infected cells. Cell Microbiol. 2021 Jun;23(6):e13322. doi: 10.1111/cmi.13322. Epub 2021 Mar 9.
  • Freund C, Schwarzer D. Engineered Sortases in Peptide and Protein Chemistry. Chembiochem. 2021 Apr 16;22(8):1347-1356. doi: 10.1002/cbic.202000745. Epub 2021 Feb 3.

2020

  • Álvaro-Benito M, Freund C. Revisiting nonclassical HLA II functions in antigen presentation: Peptide editing and its modulation. HLA. 2020 Oct;96(4):415-429. doi: 10.1111/tan.14007. Epub 2020 Aug 27.
  • Preussner M, Gao Q, Morrison E, Herdt O, Finkernagel F, Schumann M, Krause E, Freund C, Chen W, Heyd F. Splicing-accessible coding 3'UTRs control protein stability and interaction networks. Genome Biol. 2020 Jul 29;21(1):186
  • Graves AM, Virdis F, Morrison E, Álvaro-Benito M, Khan AA, Freund C, Golovkina TV, Denzin LK. Human Hepatitis B Viral Infection Outcomes Are Linked to Naturally Occurring Variants of HLA-DOA That Have Altered Function. J Immunol. 2020 Jul 20:ji2000476. doi: 10.4049/jimmunol.2000476. Online ahead of print.
  • Morrison E, Wegner T, Zucchetti AE, Álvaro-Benito M, Zheng A, Kliche S, Krause E, Brügger B, Hivroz C, Freund C. Dynamic palmitoylation events following T-cell receptor signaling. Commun Biol. 2020 Jul 10;3(1):368. doi: 10.1038/s42003-020-1063-5.
  • Vardar G, Gerth F, Schmitt XJ, Rautenstrauch P, Trimbuch T, Schubert J, Lerche H, Rosenmund C, Freund C. Epilepsy-causing STX1B mutations translate altered protein functions into distinct phenotypes in mouse neurons. Brain. 2020 143:2119-2138. doi: 10.1093/brain/awaa151

  • Petzoldt AG, Götz TWB, Driller JH, Lützkendorf J, Reddy-Alla S, Matkovic-Rachid T, Liu S, Knoche E, Mertel S, Ugorets V, Lehmann M, Ramesh N, Beuschel CB, Kuropka B, Freund C, Stelzl U, Loll B, Liu F, Wahl MC, Sigrist SJ. RIM-binding protein couples synaptic vesicle recruitment to release sites. J Cell Biol. 2020 Jul 6;219(7):e201902059. doi: 10.1083/jcb.201902059.

  • Legrand C, Saleppico R, Sticht J, Lolicato F, Müller HM, Wegehingel S, Dimou E, Steringer JP, Ewers H, Vattulainen I, Freund C, Nickel W. The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2. Commun Biol. 2020 Mar 25;3(1):141. doi: 10.1038/s42003-020-0871-y.

  • Ebner F, Morrison E, Bertazzon M, Midha A, Hartmann S, Freund C, Álvaro-Benito M. CD4+ Th immunogenicity of the Ascaris spp. secreted products. NPJ Vaccines. 2020 Mar 20;5:25. doi: 10.1038/s41541-020-0171-z. eCollection 2020.

  • Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V. Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission. Cell Rep. 2020 Jan 14;30(2):409-420.e6. doi: 10.1016/j.celrep.2019.12.035.

2019

  • Freund C, Höfer T.A Missing Switch in Peptide Exchange for MHC Class II Molecules. Front Immunol. 2019. eCollection 2019. No abstract available.
  • Gerth F, Jäpel M, Sticht J, Kuropka B, Schmitt XJ, Driller JH, Loll B, Wahl MC, Pagel K, Haucke V, Freund C. 2019. Exon Inclusion Modulates Conformational Plasticity and Autoinhibition of the Intersectin 1 SH3A Domain. Structure. Jun 4;27(6):977-987.e5. Epub 2019 Apr 25.PMID:31031201
  • Rademacher N, Kuropka B, Kunde SA, Wahl MC, Freund C, Shoichet SA. (2019) Intramolecular domain dynamics regulate synaptic MAGUK protein interactions. Elife. 2019 Mar 13;8. pii: e41299. doi: 10.7554/eLife.41299.PMID:30864948
  • Driller JH, Lützkendorf J, Depner H, Siebert M, Kuropka B, Weise C, Piao C, Petzoldt AG, Lehmann M, Stelzl U, Zahedi R, Sickmann A, Freund C, Sigrist SJ, Wahl MC. (2019). Phosphorylation of the Bruchpilot N-terminus in Drosophila unlocks axonal transport of active zone building blocks..J Cell Sci. 2019 Mar 18;132(6). pii: jcs225151. doi: 10.1242/jcs.225151. PMID:30745339
  • Rödel CJ, Otten C, Donat S, Lourenço M, Fischer D, Kuropka B, Paolini A, Freund C, Abdelilah-Seyfried S. Blood Flow Suppresses Vascular Anomalies in a Zebrafish Model of Cerebral Cavernous Malformations. Circ Res. 2019 Oct 25;125(10):e43-e54. doi: 10.1161/CIRCRESAHA.119.315076. Epub 2019 Sep 9. PMID: 31495257
  • Sticht J, Bertazzon M, Henning LM, Licha JR, Abualrous ET, Freund C. FBP21's C-Terminal Domain Remains Dynamic When Wrapped around the c-Sec63 Unit of Brr2 Helicase. Biophys J. 2019 Feb 5;116(3):406-418. doi:10.1016/j.bpj. 2018.11.3123. Epub 2018 Nov 29. PMID: 30558886

2018

  • Kroczek AL, Hartung E, Gurka S, Becker M, Reeg N, Mages HW, Voigt S, Freund C, Kroczek RA. Structure-Function Relationship of XCL1 Used for in vivo Targeting of Antigen Into XCR1+ Dendritic Cells. Front Immunol. 2018 Dec 13;9:2806. doi: 10.3389/fimmu.2018.02806. eCollection 2018. PMID:30619244
  • Waldt N, Seifert A, Demiray YE, Devroe E, Turk BE, Reichardt P, Mix C, Reinhold A, Freund C, Müller AJ, Schraven B, Stork O, Kliche S. Filamin A Phosphorylation at Serine 2152 by the Serine/Threonine Kinase Ndr2 Controls TCR-Induced LFA-1 Activation in T Cells. Front Immunol. 2018 Dec 4;9:2852. doi: 10.3389/ fimmu.2018.02852. eCollection 2018.
  • Álvaro-Benito M, Morrison E, Ebner F, Abualrous ET, Urbicht M, Wieczorek M, Freund C. Distinct editing functions of natural HLA-DM allotypes impact antigen presentation and CD4+ T cell activation. Cell Mol Immunol. 2018 Nov 22. doi: 10.1038/s41423-018-0181-1. [Epub ahead of print]
  • Werno MW, Wilhelmi I, Kuropka B, Ebert F, Freund C, Schürmann A. The GTPase ARFRP1 affects lipid droplet protein composition and triglyceride release from intracellular storage of intestinal Caco-2 cells. Biochem Biophys Res Commun. 2018 Oct 19. pii: S0006-291X(18)32248-4. doi: 10.1016/j.bbrc.2018.10.092. Epub 2018 Oct 19.
  • Miceli E, Kuropka B, Rosenauer C, Osorio Blanco ER, Theune LE, Kar M, Weise C, Morsbach S, Freund C, Calderón M. Understanding the elusive protein corona of thermoresponsive nanogels. Nanomedicine (Lond). 2018 Oct 18. doi: 10.2217/nnm-2018-0217. Epub 2018 Oct 18.
  • Álvaro-Benito M, Morrison E, Abualrous ET, Kuropka B, Freund C. Quantification of HLA-DM-Dependent Major Histocompatibility Complex of Class II Immunopeptidomes by the Peptide Landscape Antigenic Epitope Alignment Utility. Front Immunol. 2018 May 3;9:872. doi: 10.3389/fimmu.2018.00872. eCollection 2018.
  • Wollenhaupt J, Henning LM, Sticht J, Becke C, Freund C, Santos KF, Wahl MC. Intrinsically Disordered Protein Ntr2 Modulates the Spliceosomal RNA Helicase Brr2. Biophys J. 2018 Feb 27;114(4):788-799. doi: 10.1016/j.bpj.2017.12.033.

2017

  • A Coincidence Detection Mechanism Controls PX-BAR Domain-Mediated Endocytic Membrane Remodeling via an Allosteric Structural Switch.
    Lo WT, Vujičić Žagar A, Gerth F, Lehmann M, Puchkov D, Krylova O, Freund C, Scapozza L, Vadas O, Haucke V.
    Dev Cell. 2017; 43, 522-529.

  • Intersectin associates with synapsin and regulates its nanoscale localization and function.
    Gerth F, Jäpel M, Pechstein A, Kochlamazashvili G, Lehmann M, Puchkov D, Onofri F, Benfenati F, Nikonenko AG, Fredrich K, Shupliakov O, Maritzen T, Freund C, Haucke V.
    Proc Natl Acad Sci U S A. 2017; 114, 12057-12062.

  • Protein-peptide association kinetics beyond the seconds timescale from atomistic simulations.
    Paul F, Wehmeyer C, Abualrous ET, Wu H, Crabtree MD, Schöneberg J, Clarke J, Freund C, Weikl TR, Noé F.
    Nat. Commun. 2017 8, 1095.

  • A new role for FBP21 as regulator of Brr2 helicase activity.
    Henning LM, Santos KF, Sticht J, Jehle S, Lee CT, Wittwer M, Urlaub H, Stelzl U, Wahl MC, Freund C.
    Nucleic Acids Res. 2017; 45, 7922-7937.

  • Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.
    Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W.
    Elife 2017; 6. pii: e28985.

  • The Guanine-Nucleotide Exchange Factor Caldag Gefi Fine-Tunes Functional Properties of Regulatory T Cells.
    Niemz J, Kliche S, Pils MC, Morrison E, Manns A, Freund C, Crittenden JR, Graybiel AM, Galla M, Jänsch L, Huehn J.
    Eur J Microbiol Immunol 2017; (Bp). 7, 112-126.

  • Identification of sortase substrates by specificity profiling.
    Schmohl L, Bierlmeier J, von Kügelgen N, Kurz L, Reis P, Barthels F, Mach P, Schutkowski M, Freund C, Schwarzer D.
    Bioorg Med Chem. 2017; 25, 5002-5007.

  • Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation.
    Wieczorek M, Abualrous ET, Sticht J, Álvaro-Benito M, Stolzenberg S, Noé F, Freund C.
    Front Immunol. 2017; 8, 292.

  • Noninvasive Imaging of Human Immune Responses in a Human Xenograft Model of Graft-Versus-Host Disease.
    Van Elssen CHMJ, Rashidian M, Vrbanac V, Wucherpfennig KW, Habre ZE, Sticht J, Freund C, Jacobsen JT, Cragnolini J, Ingram J, Plaisier L, Spierings E, Tager AM, Ploegh HL.
    J Nucl. Med 2017; 58(6):1003-1008.

  • Engineering sortase A by screening a second-generation library using phage display.
    Schmohl L, Bierlmeier J, Gerth F, Freund C, Schwarzer D.
    J Pept Sci. 2017; 10.1002/psc.2980

  • The differentiation and plasticity of Tc17 cells are regulated by CTLA-4-mediated effects on STATs.
    Arra A, Lingel H, Kuropka B, Pick J, Schnoeder T, Fischer T, Freund C, Pierau M, Brunner-Weinzierl MC.
    Oncoimmunology 2017; 6(2): e1273300.

  • D120 and K152 within the PH Domain of T Cell Adapter SKAP55 Regulate Plasma Membrane Targeting of SKAP55 and LFA-1 Affinity Modulation in Human T Lymphocytes.
    Witte A, Meineke B, Sticht J, Philipsen L, Kuropka B, Müller AJ, Freund C, Schraven B, Kliche S.
    Mol Cell Biol 2017; 37(7): e00509-16.

2016

  • MHC class II complexes sample intermediate states along the peptide exchange pathway.
    Wieczorek M, Sticht J, Stolzenberg S, Günther S, Wehmeyer C, El Habre Z, Álvaro-Benito M, Noé F, Freund C.
    Nat Commun. 2016 Nov 9;7:13224.

  • Human leukocyte Antigen-DM polymorphisms in autoimmune diseases.
    Alvaro-Benito M, Morrison E, Wieczorek M, Sticht J, Freund C.
    Open Biol.     2016 Aug;6(8).

  • Tyrosine-phosphorylation of the scaffold protein ADAP and its role in T cell signaling.
    Kuropka B, Schraven B, Kliche S, Krause E, Freund C
    Expert Rev Proteomics    . 2016 Jun;13(6):545-54.

2015

  • Analysis of Phosphorylation-dependent Protein Interactions of Adhesion and Degranulation Promoting Adaptor Protein (ADAP) Reveals Novel Interaction Partners Required for Chemokine-directed T cell Migration.
    Kuropka B, Witte A, Sticht J, Waldt N, Majkut P, Hackenberger CP, Schraven B, Krause E, Kliche S, Freund C
    Mol Cell Proteomics.     2015 Nov 1; 14:2961-72.

  • The GYF domain protein CD2BP2 is critical for embryogenesis and podocyte function.
    Albert GI, Schell C, Kirschner KM, Schäfer S, Naumann R, Müller A, Kretz O, Kuropka B, Girbig M, Hübner N, Krause E, Scholz H, Huber TB, Knobeloch KP, Freund C
    J Mol Cell Biol.     2015 Oct; 7:402-14

  • Quantitative analysis of the human T cell palmitome.
    Morrison, E., Kuropka, B., Kliche, S., Krause, E. and Freund, C.
    Sci. Rep.    , 2015 Jun 26;5:11598.

  • Peptide-polymer ligands for a tandem WW-domain, an adaptive multivalent protein-protein interaction: lessons on the thermodynamic fitness of flexible ligands.
    Koschek K, Durmaz V, Krylova O, Wieczorek M, Gupta S, Richter M, Bujotzek A, Fischer C, Haag R, Freund C, Weber M, Rademann J.
    Beilstein J Org Chem.    , 2015 May 18;11:837-47.

  • Exploring monovalent and multivalent peptides for the inhibition of FBP21-tWW.
    Henning, L. M., Bhatia, S., Bertazzon, M., Marczynke, M., Seitz, O., Volkmer, R., Haag, R. and Freund, C.
    Beilstein J. Org. Chem.    ,  2015 May 11;11:701-6.

  • ProMs: A modular toolkit to inhibit proline-rich motif mediated protein-protein interactions.
    Opitz, R., Müller, M., , C., Barone, M., Soicke, A., Roske, Y., Piotukh, K., Huy, P., Beerbaum, M., Wiesner, B., Beyermann, M., Schmieder, P., Freund, C., Volkmer, R., Oschkinat, H., Schmalz, H.-G. & Kühne, R.
    Proc. Natl. Acad. Sci.     (USA), 2015 Apr 21;112(16):5011-6.

  • Sortase A mediated site-specific immobilization for identification of protein interactions in affinity purification-mass spectrometry experiments.
    Kuropka B, Royla N, Freund C, Krause E.
    Proteomics    . 2015 Apr;15(7):1230-4.

  • Vesicle uncoating regulated by SH3-SH3 domain-mediated complex formation between endophilin and intersectin at synapses
    Pechstein, A., Gerth, F., Milosevic, I., Jäpel, M., Eichhorn-Grünig, M., Vorontsova, O., Bacetic, J., Maritzen, T., Shupliakov, O., Freund, C. & Haucke, V.
    EMBO Rep. 2015 Feb;16(2):232-9.

  • HLA-DMA polymorphisms differentially affect MHC class II peptide loading.
    Álvaro-Benito, M., Wieczorek, M., Sticht, J., Kipar, C. & Freund, C.
    J Immunol. 2015 Jan 15;194(2):803-16.

2014

  • Multichannel MRI Labeling of Mammalian Cells by Switchable Nanocarriers for Hyperpolarized Xenon.
    Klippel S, Freund C, Schröder L.
    Nano Lett. 2014 Sep 30. [Epub ahead of print]

  • Development of an antibody-based, modular biosensor for 129Xe NMR molecular imaging of cells at nanomolar concentrations.
    Rose HM, Witte C, Rossella F, Klippel S, Freund C, Schröder L.
    Proc Natl Acad Sci U S A. 2014 Aug 12;111(32):11697-702.

  • Susceptibility to HLA-DM Protein Is Determined by a Dynamic Conformation of Major Histocompatibility Complex Class II Molecule Bound with Peptide.
    Yin L, Trenh O, Wieczorek M, Lange S, Sticht J, Jiang W, Bylsma M, Mellins ED, Freund C, Stern LJ.
    J Biol Chem.     2014 Aug 22; 289(34):23449-64

  • The serine/threonine kinase Ndr2 controls integrin trafficking and integrin-dependent neurite growth.
    Rehberg K, Kliche S, Madencioglu DA, Thiere M, Müller B, Meineke BM, Freund C, Budinger E, Stork O.
    J Neurosci. 2014 Apr 9;34(15):5342-54.

2013

  • Completion of proteomic data sets by Kd measurement using cell-free synthesis of site-specifically labeled proteins.
    Majkut P, Claußnitzer I, Merk H, Freund C, Hackenberger CP, Gerrits M.
    PLoS One. 2013 Dec 10;8(12):e82352.

  • Cell tracking with caged xenon: using cryptophanes as MRI reporters upon cellular internalization.
    Klippel S, Döpfert J, Jayapaul J, Kunth M, Rossella F, Schnurr M, Witte C, Freund C, Schröder L.
    Angew Chem Int Ed Engl. 2014 Jan 7;53(2):493-6.

  • Probing the recognition of post-translational modifications by combining sortase-mediated ligation and phage-assisted selection.
    Teschke T, Geltinger B, Dose A, Freund C, Schwarzer D.
    ACS Chem Biol. 2013 Aug 16;8(8):1692-7.

2012

  • Phosphatidylinositol 4-kinase II function at endosomes is regulated by the ubiquitin ligase Itch.
    Mössinger, J., Wieffer, M., Krause, E., Freund, C., Gerth, F., Krauss, M. & Haucke, V.
    EMBO Reports 13, 1087-1094.

  • A novel hSH3 domain scaffold engineered to bind folded domains in CD2BP2 and HIV capsid protein.
    Piotukh, K. & Freund, C.
    Protein Eng. Des. Sel. 2012, 25:649-56.

  • Peptide linkage to the alpha-subunit of MHCII creates a stably inverted antigen presentation complex.
    Schlundt, A., Günther, S., Sticht, J., Wieczorek, M., Roske, Y., Heinemann, U. & Freund, C.
    J. Mol. Biol. 2012, 423, 294-302.

  • CCR7-mediated LFA-1 functions in T cells are regulated by 2 independent ADAP/SKAP55 modules.
    Kliche S, Worbs T, Wang X, Degen J, Patzak I, Meineke B, Togni M, Moser M, Reinhold A, Kiefer F, Freund C, Förster R, Schraven B.
    Blood. 2012 Jan 19;119(3):777-85. Epub 2011 Nov 23. PMID:22117043

  • An improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions.
    Stephanowitz, H., Lange, S., Lang, D., Freund, C., and Krause, E.
    J. Proteome Research 2012, 11(2):1175-83.

2011

  • Directed evolution of sortase A mutants with altered substrate selectivity profiles.
    Piotukh K, Geltinger B, Heinrich N, Gerth F, Beyermann M, Freund C, Schwarzer D.
    J Am Chem Soc. 2011 Nov 9;133(44):17536-9. Epub 2011 Oct 13.     

  • Multivalent binding of formin-binding protein 21 (FBP21)-tandem-WW domains fosters protein recognition in the pre-spliceosome.
    Klippel S, Wieczorek M, Schümann M, Krause E, Marg B, Seidel T, Meyer T, Knapp EW, Freund C.
    J Biol Chem. 2011 Nov 4;286(44):38478-87. Epub 2011 Sep 14.      

  • Characterization of structural features controlling the receptiveness of empty class II MHC molecules.
    Rupp B, Günther S, Makhmoor T, Schlundt A, Dickhaut K, Gupta S, Choudhary I, Wiesmüller KH, Jung G, Freund C, Falk K, Rötzschke O, Kühne R.
    PLoS One. 2011 Apr 14;6(4):e18662.     

  • Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.
    Borja MS, Piotukh K, Freund C, Gross JD.
    RNA. 2011 Feb;17(2):278-90. Epub 2010 Dec 10.

2010

  • Bidirectional binding of invariant chain peptides to an MHC class II molecule.
    Günther S, Schlundt A, Sticht J, Roske Y, Heinemann U, Wiesmüller KH, Jung G, Falk K, Rötzschke O, Freund C.
    Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22219-24. Epub 2010 Nov 29.     

  • Addressing protein-protein interactions with small molecules: a Pro-Pro dipeptide mimic with a PPII helix conformation as a module for the synthesis of PRD-binding ligands.
    Zaminer J, Brockmann C, Huy P, Opitz R, Reuter C, Beyermann M, Freund C, Müller M, Oschkinat H, Kühne R, Schmalz HG.
    Angew Chem Int Ed Engl. 2010 Sep 17;49(39):7111-5.     

  • Conserved beta-hairpin recognition by the GYF domains of Smy2 and GIGYF2 in mRNA surveillance and vesicular transport complexes.
    Ash MR, Faelber K, Kosslick D, Albert GI, Roske Y, Kofler M, Schuemann M, Krause E, Freund C.
    Structure. 2010 Aug 11;18(8):944-54.     

  • Adhesion and degranulation promoting adapter protein (ADAP) is a central hub for phosphotyrosine-mediated interactions in T cells.
    Sylvester M, Kliche S, Lange S, Geithner S, Klemm C, Schlosser A, Grossmann A, Stelzl U, Schraven B, Krause E, Freund C.
    PLoS One. 2010 Jul 22;5(7):e11708. Erratum in: PLoS One. 2010;5(8).

  • Identification of phosphorylation-dependent interaction partners of the adapter protein ADAP using quantitative mass spectrometry: SILAC vs (18)O-labeling.
    Lange S, Sylvester M, Schümann M, Freund C, Krause E.
    J Proteome Res. 2010 Aug 6;9(8):4113-22.

2009

  • A novel S3S-TAP-tag for the isolation of T-cell interaction partners of adhesion and degranulation promoting adaptor protein.
    Lehmann R, Meyer J, Schuemann M, Krause E, Freund C.
    Proteomics. 2009 Dec;9(23):5288-95.

  • Proline-rich sequence recognition: II. Proteomics analysis of Tsg101 ubiquitin-E2-like variant (UEV) interactions.
    Schlundt A, Sticht J, Piotukh K, Kosslick D, Jahnke N, Keller S, Schuemann M, Krause E, Freund C.
    Mol Cell Proteomics. 2009 Nov;8(11):2474-86.

  • Proline-rich sequence recognition: I. Marking GYF and WW domain assembly sites in early spliceosomal complexes.
    Kofler M, Schuemann M, Merz C, Kosslick D, Schlundt A, Tannert A, Schaefer M, Lührmann R, Krause E, Freund C.
    Mol Cell Proteomics. 2009 Nov;8(11):2461-73.

  • A xenon-129 biosensor for monitoring MHC-peptide interactions.
    Schlundt A, Kilian W, Beyermann M, Sticht J, Günther S, Höpner S, Falk K, Roetzschke O, Mitschang L, Freund C.
    Angew Chem Int Ed Engl. 2009;48(23):4142-5.

2008

  • Resin-bound aminofluorescein for C-terminal labeling of peptides: high-affinity polarization probes binding to polyproline-specific GYF domains.
    Uryga-Polowy V, Kosslick D, Freund C, Rademann J.
    Chembiochem. 2008 Oct 13;9(15):2452-62.

  • Proline-rich sequence recognition domains (PRD): ligands, function and inhibition.
    Freund C, Schmalz HG, Sticht J, Kühne R.
    Handb Exp Pharmacol. 2008;(186):407-29. Review.

  • Mutational analyses of c-FLIPR, the only murine short FLIP isoform, reveal requirements for DISC recruitment.
    Ueffing N, Keil E, Freund C, Kühne R, Schulze-Osthoff K, Schmitz I.
    Cell Death Differ. 2008 Apr;15(4):773-82. 

  • Analysis of the substrate specificity of the Dim-5 histone lysine methyltransferase using peptide arrays.
    Rathert P, Zhang X, Freund C, Cheng X, Jeltsch A.
    Chem Biol. 2008 Jan;15(1):5-11.

2007

  • Investigating the functional role of CD2BP2 in T cells.
    Heinze M, Kofler M, Freund C.
    Int Immunol. 2007 Nov;19(11):1313-8.

  • Reversible disulfide bond formation of intracellular proteins probed by NMR spectroscopy.
    Piotukh K, Kosslick D, Zimmermann J, Krause E, Freund C.
    Free Radic Biol Med. 2007 Nov 1;43(9):1263-70.

  • Redox-regulated conformational changes in an SH3 domain.
    Zimmermann J, Kühne R, Sylvester M, Freund C.
    Biochemistry. 2007 Jun 12;46(23):6971-7.

2006

  • Small organic compounds enhance antigen loading of class II major histocompatibility complex proteins by targeting the polymorphic P1 pocket.
    Höpner S, Dickhaut K, Hofstätter M, Krämer H, Rückerl D, Söderhäll JA, Gupta S, Marin-Esteban V, Kühne R, Freund C, Jung G, Falk K, Rötzschke O.
    J Biol Chem. 2006 Dec 15;281(50):38535-42.  

  • The ADAP/SKAP55 signaling module regulates T-cell receptor-mediated integrin activation through plasma membrane targeting of Rap1.
    Kliche S, Breitling D, Togni M, Pusch R, Heuer K, Wang X, Freund C, Kasirer-Friede A, Menasche G, Koretzky GA, Schraven B.
    Mol Cell Biol. 2006 Oct;26(19):7130-44.

  • Lipid-binding hSH3 domains in immune cell adapter proteins.
    Heuer K, Sylvester M, Kliche S, Pusch R, Thiemke K, Schraven B, Freund C.
    J Mol Biol. 2006 Aug 4;361(1):94-104.

  • The GYF domain.
    Kofler MM, Freund C.
    FEBS J. 2006 Jan;273(2):245-56. Review.

2005

  • GYF domain proteomics reveals interaction sites in known and novel target proteins.
    Kofler M, Motzny K, Freund C.
    Mol Cell Proteomics. 2005 Nov;4(11):1797-811.

  • NMR assignment of the reduced and oxidized forms of the human ADAP hSH3-1 domain.
    Zimmermann J, Freund C.
    J Biomol NMR. 2005 May;32(1):94.

  • Novel interaction partners of the CD2BP2-GYF domain.
    Kofler M, Motzny K, Beyermann M, Freund C.
    J Biol Chem. 2005 Sep 30;280(39):33397-402.

  • Alternative binding modes of proline-rich peptides binding to the GYF domain.
    Gu W, Kofler M, Antes I, Freund C, Helms V.
    Biochemistry. 2005 May 3;44(17):6404-15.

  • Cyclophilin A binds to linear peptide motifs containing a consensus that is present in many human proteins.
    Piotukh K, Gu W, Kofler M, Labudde D, Helms V, Freund C.
    J Biol Chem. 2005 Jun 24;280(25):23668-74.

  • The helically extended SH3 domain of the T cell adaptor protein ADAP is a novel lipid interaction domain.
    Heuer K, Arbuzova A, Strauss H, Kofler M, Freund C.
    J Mol Biol. 2005 May 13;348(4):1025-35.

  • Statistically significant dependence of the Xaa-Pro peptide bond conformation on secondary structure and amino acid sequence.
    Pahlke D, Freund C, Leitner D, Labudde D.
    BMC Struct Biol. 2005 Apr 1;5:8.

2004

  • Recognition sequences for the GYF domain reveal a possible spliceosomal function of CD2BP2.
    Kofler M, Heuer K, Zech T, Freund C.
    J Biol Chem. 2004 Jul 2;279(27):28292-7.

  • Structure of a helically extended SH3 domain of the T cell adapter protein ADAP.
    Heuer K, Kofler M, Langdon G, Thiemke K, Freund C.
    Structure. 2004 Apr;12(4):603-10.