The Honeybee Standard Brain Atlas is calculated from 20 individual bee brain images that were immuno-stained, imaged as whole-mounts with the confocal microscope, and segmented along the borderlines of 22 neuropils. After correcting for global size and positioning differences, an average label image was created by repeated application of an intensity-based non-rigid registration algorithm. A three-dimensional surface model was reconstructed from this.
Early applications of the Honeybee Standard Brain demonstrate the mapping procedure of individual electrophysiologically-recorded and intracellularly-stained olfactory interneurons. A detailed digital Atlas of the Honeybee antennal lobe is available and will be integrated into the Virtual Atlas of the Honeybee Brain.