Differentiating N-acetylneuraminic acid linkage isomers on glycopeptides
News from Feb 25, 2016
Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions.
go to article DOI: 10.1039/C6CC01114D