Collision cross sections of high-mannose N-glycans in commonly observed adduct states - Identification of gas-phase conformers unique to [M-H]^- ions

We report collision cross sections (CCS) of high-mannose N-glycans as [M+Na]⁺, [M+K]⁺, [M+H]⁺, [M+Cl]^-, [M+H2PO4]^- and [M-H]^- ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M-H]^- ions.

go to article DOI: 10.1039/C5AN01092F