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539. Responsive Emulsions for Sequential Multienzyme Cascades

Z. Sun, Q. Zhao, R. Haag, C. Wu – 2021

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

Title
539. Responsive Emulsions for Sequential Multienzyme Cascades
Author
Z. Sun, Q. Zhao, R. Haag, C. Wu
Date
2021
Identifier
DOI: 10.1002/anie.202013737
Source(s)
Citation
Angew. Chem. Int. Ed. Engl., 2021, 60 (15), 8410-8414
Type
Text