Schwerpunkte meiner Lehre sind:

• Vorlesungen: Immunologie (5.Sem.), Pharmazeutische Biologie Ringvorlesung (6.-8. Sem.)
• Praktika: Zytologie (2.Sem.), Bio III (6.Sem.)
• Seminare: Phytotherapie (7. + 8. Sem.)
• Wahlpflichtfach (WPF) in der Pharmazeutischen Biologie: Interessenten/Interessentinnen für das WPF melden sich bitte per Mail direkt bei mir oder den Doktoranden/Doktorandinnen meiner Arbeitsgruppe. Da wir vorwiegend experimentelle Arbeiten durchführen, sollte das Praktikum BIO III im 6. Semester bereits erfolgreich absolviert sein. Die Zuordnung der Themen und Absprachen zu den Terminen erfolgt jeweils zum Semesterende nach Aufforderung durch mich in der Ringvorlesung und nach Terminabsprache mit mir.

Themenübersicht für die Ringvorlesung Pharmazeutische Biologie

Meine Arbeitsgruppe beschäftigt sich mit der Isolation, chemischen Charakterisierung und den pharmakologischen Wirkungen von Naturstoffen aus höheren Pflanzen. Ein Schwerpunkt bildet dabei die Untersuchung von Arzneipflanzen, die in der Volksheilkunde seit langer Zeit eingesetzt werden, über deren biologischen Wirkungen aber bisher sehr wenig bekannt ist. Weiterhin untersuchen wir den Einsatz von Naturstoffen als Enzymhemmer  (u.a. Proteasen, Amylasen) sowie die molekularen Wirkungsmechanismen von ausgewählten Triterpenderivaten.

Our research is focused on isolation, chemical characterization and investigation in pharmacological activities of natural products of higher plants. The emphasis is on the investigation of medicinal plants used in traditional and folk  medicine to elucidate their biological activity and mode of action. Especially we are interested in finding new inhibitor of enzymes (proteases, amylases, lipases and other hydrolases) as well as the mode of action of selected triterpenoid compounds, mainly saponins and triterpenoic acids.

Detailed research topics and technologies.

(2022)

Sehner SM, Melzig MF. Pharmazie. 2022 Jan 3;77(1):2-5. doi: 10.1691/ph.2022.1135. Fluorometric quantification of alkaloids in the homeopathic mother tinctures of Vinca minor L. and Fumaria officinalis L.

Olędzka A, Cichocka K, Woliński K, Melzig MF, Czerwińska ME. Nutrients. 2022 May 30;14(11):2287. doi: 10.3390/nu14112287. Potentially Bio-Accessible Metabolites from an Extract of Cornus mas Fruit after Gastrointestinal Digestion In Vitro and Gut Microbiota Ex Vivo Treatment.

Bunse M, Daniels R, Gründemann C, Heilmann J, Kammerer DR, Keusgen M, Lindequist U, Melzig MF, Morlock GE, Schulz H, Schweiggert R, Simon M, Stintzing FC, Wink M. Front Pharmacol. 2022 Aug 24; 13:956541. doi: 10.3389/fphar.2022.956541. Essential Oils as Multicomponent Mixtures and Their Potential for Human Health and Well-Being.

Laskowska AK, Wilczak A, Skowrońska W, Michel P, Melzig MF, Czerwińska ME. Front Pharmacol. 2022 Sep 30; 13:981874. doi: 10.3389/fphar.2022.981874. Fruits of Hippophaë rhamnoides in human leukocytes and Caco-2 cell monolayer models-A question about their preventive role in lipopolysaccharide leakage and cytokine secretion in endotoxemia.

 

(2021)

Bobowska A, Granica S, Filipek A, Melzig MF, Moeslinger T, Zentek J, Kruk A, Piwowarski JP. Eur J Nutr. 2021 Jun;60(4):1957-1972. doi: 10.1007/s00394-020-02386-y. Comparative studies of urolithins and their phase II metabolites on macrophage and neutrophil functions.

Granica S, Vahjen W, Zentek J, Melzig MF, Pawłowska KA, Piwowarski JP. J Nat Prod. 2020 Dec 24;83(12):3614-3622. doi: 10.1021/acs.jnatprod.0c00776. Lythrum salicaria Ellagitannins Stimulate IPEC-J2 Cells Monolayer Formation and Inhibit Enteropathogenic Escherichia coli Growth and Adhesion.

Popowski D, Czerwińska ME, Kruk A, Pawłowska KA, Zentek J, Melzig MF, Piwowarski JP, Granica S. J Ethnopharmacol. 2021 Jun 12; 273:113924. doi: 10.1016/j.jep.2021.113924. Gut microbiota metabolism and the permeability of natural products contained in infusions from herb of European goldenrod Solidago virgaurea L.

Popowski D, Pawłowska KA, Deipenbrock M, Hensel A, Kruk A, Melzig MF, Piwowarski JP, Granica S. J Ethnopharmacol. 2021 Jun 28; 274:114053. doi: 10.1016/j.jep.2021.114053. Antiadhesive activity of hydroethanolic extract from bean pods of Phaseolus vulgaris (common bean) against uropathogenic E. coli and permeability of its constituents through Caco-2 cells    monolayer.

Siegień J, Buchholz T, Popowski D, Granica S, Osińska E, Melzig MF, Czerwińska ME. Food Chem. 2021 Sep 1; 355:129414. doi: 10.1016/j.foodchem.2021.129414. Pancreatic lipase and α-amylase inhibitory activity of extracts from selected plant materials after gastrointestinal digestion in vitro.

Woith E, Guerriero G, Hausman JF, Renaut J, Leclercq CC, Weise C, Legay S, Weng A, Melzig MF. Int J Mol Sci. 2021 Apr 2;22(7):3719. doi: 10.3390/ijms22073719. Plant Extracellular Vesicles and Nanovesicles: Focus on Secondary Metabolites, Proteins and Lipids with Perspectives on Their Potential and Sources.

Fouedjou RT, Ponou BK, Teponno RB, Melzig M, Tanaka C, Miyamoto T, Tapondjou LA. Nat Prod Res. 2022 Oct;36(19):4949-4956. doi: 10.1080/14786419.2021.1914030. Two new triterpenoid saponins: telephiifoliosides A and B from the roots of Corrigiola litoralis subsp. telephiifolia (Pourr.) Briq.

Farinacci P, Mevissen M, Ayrle H, Maurer V, Sørensen Dalgaard T, Melzig MF, Walkenhorst M. Planta Med. 2022 Mar;88(3-04):200-217. doi: 10.1055/a-1543-5502. Medicinal Plants for Prophylaxis and Therapy of Common Infectious Diseases in Poultry- A Systematic Review of In Vivo Studies.

Schreck K, Melzig MF. Front Pharmacol. 2021 Aug 20; 12:692566. doi: 10.3389/fphar.2021.692566. Traditionally Used Plants in the Treatment of Diabetes Mellitus: Screening for Uptake Inhibition of Glucose and Fructose in the Caco2-Cell Model.

Czerwińska ME, Bobińska A, Cichocka K, Buchholz T, Woliński K, Melzig MF. Plants (Basel). 2021 Oct 30;10(11):2347. doi: 10.3390/plants10112347. Cornus mas and Cornus officinalis-A Comparison of Antioxidant and Immunomodulatory Activities of Standardized Fruit Extracts in Human Neutrophils and Caco-2 Models.

 

(2020)

Truba J, Stanisławska I, Walasek M, Wieczorkowska W, Woliński K, Buchholz T, Melzig MF, Czerwińska ME. Plants (Basel). 9 (2020) pii: E122. Inhibition of Digestive Enzymes and Antioxidant Activity of Extracts from Fruits of Cornus alba, Cornus sanguinea subsp. hungarica and Cornus florida-A Comparative Study.

Melzig MF, Böttger S. Planta Med. 2020 Mar 10. doi: 10.1055/a-1129-7214. Tormentillae rhizoma - Review for an Underestimated European Herbal Drug.

Yu L, Hou Y, Xie W, Camacho JLC, Cheng C, Holle A, Young J, Trappmann B, Zhao W, Melzig MF, Cavalcanti-Adam EA, Zhao C, Spatz JP, Wei Q, Haag R. Adv Mater. 2020 Jul;32(29): e2002566. doi: 10.1002/adma.202002566. Ligand Diffusion Enables Force-Independent Cell Adhesion via Activating α5β1 Integrin and Initiating Rac and RhoA Signaling.

Dadi TH, Vahjen W, Zentek J, Melzig MF, Granica S, Piwowarski JP. J Ethnopharmacol. 2020 Oct 28; 261:113073. doi: 10.1016/j.jep.2020.113073. Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets. Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.

Weise C, Schrot A, Wuerger LTD, Adolf J, Gilabert-Oriol R, Sama S, Melzig MF, Weng A. Sci Rep. 2020 Sep 21;10(1):15377. doi: 10.1038/s41598-020-72282-2. An unusual type I ribosome-inactivating protein from Agrostemma githago L.

 

(2019)

Świerczewska A, Buchholz T, Melzig MF, Czerwińska ME. J Food Drug Anal. 27 (2019) 249-258. In vitro α-amylase and pancreatic lipase inhibitory activity of Cornus mas L. and Cornus alba L. fruit extracts.

Guenther F, Maus D, Hedtrich S, Melzig MF. Planta Med. 85 (2019) 578-582. Serine Protease Mauritanicain from Euphorbia mauritanica and Phorbol-12-myristate-13-acetate Modulate theIL-8 Release in Fibroblasts and HaCaT Keratinocytes.

Woith E, Melzig MF. Int J Mol Sci. 20 (2019) pii: E357. Extracellular Vesicles from Fresh and Dried Plants-Simultaneous Purification and Visualization Using Gel Electrophoresis.

Sama S, Jerz G, Thakur M, Melzig MF, Weng A. Planta Med. 85 (2019) 513-518. Structure-Activity Relationship of Transfection-Modulating Saponins - A Pursuit for the Optimal Gene Trafficker.

Tresch M, Mevissen M, Ayrle H, Melzig M, Roosje P, Walkenhorst M. BMC Vet Res. 15 (2019) 174. Medicinal plants as therapeutic options for topical treatment in canine dermatology? A systematic review.

Bittner M, Schenk R, Springer A, Melzig MF. Planta Med. 85 (2019) 1160-1167.

Profiles of Phenolic Acids and Triterpene Glycosides in Commercial and Cultivated Black Cohosh.

Kayangar M, Ngansop Nono R, Kühlborn J, Tchuenguem R, Ponou BK, Jenett-Siems K, Teponno RB, Dzoyem JP, Opatz T, Melzig MF, Tapondjou LA. Z Naturforsch C J Biosci. 74 (2019) 289-293. A new ursane-type triterpene oxoglucopyranoside from Crossopteryx febrifuga.

Stephany-Brassesco I, Bereswill S, Heimesaat MM, Melzig MF. Eur J Microbiol Immunol (Bp). 9 (2019) 100-104. Synergistic Antimicrobial Effects of Cefabronchin^®.

Woith E, Fuhrmann G, Melzig MF. Int J Mol Sci. 20 (2019) pii: E5695. Extracellular Vesicles-Connecting Kingdoms.

 

(2018)

Sama S, Woith E, Walther W, Jerz G, Chen W, Hart S, Melzig MF, Weng A. J Control Release. 275 (2018) 208-216. Targeted suicide gene transfections reveal promising results in nu/nu mice with aggressive neuroblastoma.

Xie W, Melzig MF. Molecules. 23 (2018) pii: E919. The Stability of Medicinal Plant microRNAs in the Herb Preparation Process.

Hou Y, Xie W, Achazi K, Cuellar-Camacho JL, Melzig MF, Chen W, Haag R. Acta Biomater. 77 (2018) 28-37. Injectable degradable PVA microgels prepared by microfluidic technology for controlled osteogenic differentiation of mesenchymal stem cells.

Sama S, Jerz G, Schmieder P, Joseph JF, Melzig MF, Weng A. J Biotechnol. 284 (2018) 131-139. Plant derived triterpenes from Gypsophila elegans M.Bieb. enable non-toxic delivery of gene loaded nanoplexes.

Czerwińska ME, Melzig MF. Front Pharmacol. 9 (2018) 894. Cornus mas and Cornus officinalis-Analogies and Differences of Two Medicinal Plants Traditionally Used.

Schreck K, Melzig MF. Molecules. 23 (2018) pii: E2544. Intestinal Saturated Long-Chain Fatty Acid, Glucose and Fructose Transporters and Their Inhibition by Natural Plant Extracts in Caco-2 Cells. Correction in Molecules. 23 (2018) pii: E3290.

 

(2017)

Flemmig M, Domsalla A, Rawel H, Melzig MF. Planta Med. 83 (2017) 551-556. Isolation and Characterization of Mauritanicain, a Serine Protease from the Latex of Euphorbia mauritanica L.

Tapondjou LA, Jenett-Siems K, Siems K, Weng A, Melzig MF. Nat Prod Commun. 12 (2017) 347-350. Flavonol Glycosides and Cytotoxic Steroidal Saponins from Furcraea tuberosa (Agavaceae).

Aierken A, Buchholz T, Chen C, Zhang X, Melzig MF. J Sci Food Agric. 97 (2017) 4557-4561. Hypoglycemic effect of hawthorn in type II diabetes mellitus rat model.

Bittner M, Krähmer A, Schenk R, Springer A, Gudi G, Melzig MF. Planta Med. 83 (2017) 1085-1096. NIR Spectroscopy of Actaea racemosa L. rhizome - En Route to Fast and Low-Cost Quality Assessment.

Tchegnitegni BT, Teponno RB, Jenett-Siems K, Melzig MF, Miyamoto T, Tapondjou LA. Z Naturforsch C. 72 (2017) 477-482. A dihydrochalcone derivative and further steroidal saponins from Sansevieria trifasciata Prain.

Bhargava C, Dürkop H, Zhao X, Weng A, Melzig MF, Fuchs H. Mol Oncol. 11 (2017) 1527-1543. Targeted dianthin is a powerful toxin to treat pancreatic carcinoma when applied in combination with the glycosylated triterpene SO1861.

Sama S, Jerz G, Schmieder P, Woith E, Melzig MF, Weng A. Int J Pharm. 534 (2017) 195-205. Sapofectosid - Ensuring non-toxic and effective DNA and RNA delivery.

Xie W, Adolf J, Melzig MF. PLoS One. 12 (2017) e0187776. Identification of Viscum album L. miRNAs and prediction of their medicinal values.

Woith E, Stintzing F, Melzig MF. Pharmazie. 72 (2017) 490-496. SOD activity and extremophilicity: a screening of various plant species.