Peer-reviewed journal articles
2024
[206]
P. P. Adhikary, T. Idowu, Z. Tan, C. Hoang, S. Shanta, M. Dumbani, L. Mappalakayil, B. Awasthi, M. Bermudez, J. Weiner, D. Beule, G. Wolber, B. D. G. Page, and S. Hedtrich. Disrupting TSLP–TSLP receptor interactions via putative small molecule inhibitors yields a novel and efficient treatment option for atopic diseases, EMBO Molecular Medicine, :1-27-27, 2024.
Links:
[doi:10.1038/s44321-024-00085-3]
[show BibTeX]
[show abstract]
x
@article{RN351,
author = {Adhikary, Partho Protim and Idowu,
Temilolu and Tan, Zheng and Hoang,
Christopher and Shanta, Selina and
Dumbani, Malti and Mappalakayil, Leah and
Awasthi, Bhuwan and Bermudez, Marcel and
Weiner, January and Beule, Dieter and
Wolber, Gerhard and Page, Brent D. G. and
Hedtrich, Sarah},
title = {Disrupting TSLP–TSLP receptor
interactions via putative small molecule
inhibitors yields a novel and efficient
treatment option for atopic diseases},
journal = {EMBO Molecular Medicine},
pages = {1-27-27},
abstract = {AbstractThymic stromal lymphopoietin
(TSLP) is a key player in atopic diseases,
which has sparked great interest in
therapeutically targeting TSLP. Yet, no
small-molecule TSLP inhibitors exist due
to the challenges of disrupting the
protein?protein interaction between TSLP
and its receptor. Here, we report the
development of small-molecule TSLP
receptor inhibitors using virtual
screening and docking of 1,000,000
compounds followed by iterative chemical
synthesis. BP79 emerged as our lead
compound that effectively abrogates
TSLP-triggered cytokines at low micromolar
concentrations. For in-depth analysis, we
developed a human atopic disease drug
discovery platform using multi-organ
chips. Here, topical application of BP79
onto atopic skin models that were
co-cultivated with lung models and Th2
cells effectively suppressed immune cell
infiltration and IL-13, IL-4, TSLP, and
periostin secretion, while upregulating
skin barrier proteins. RNA-Seq analysis
corroborate these findings and indicate
protective downstream effects on the
lungs. To the best of our knowledge, this
represents the first report of a potent
putative small molecule TSLPR inhibitor
which has the potential to expand the
therapeutic and preventive options in
atopic diseases.},
ISSN = {1757-4676},
DOI = {10.1038/s44321-024-00085-3},
url = {https://doi.org/10.1038/s44321-024-00085-3},
year = {2024},
type = {Journal Article}
}
x
Disrupting TSLP–TSLP receptor interactions via putative small molecule inhibitors yields a novel and efficient treatment option for atopic diseases
AbstractThymic stromal lymphopoietin (TSLP) is a key player in atopic diseases, which has sparked great interest in therapeutically targeting TSLP. Yet, no small-molecule TSLP inhibitors exist due to the challenges of disrupting the protein?protein interaction between TSLP and its receptor. Here, we report the development of small-molecule TSLP receptor inhibitors using virtual screening and docking of >1,000,000 compounds followed by iterative chemical synthesis. BP79 emerged as our lead compound that effectively abrogates TSLP-triggered cytokines at low micromolar concentrations. For in-depth analysis, we developed a human atopic disease drug discovery platform using multi-organ chips. Here, topical application of BP79 onto atopic skin models that were co-cultivated with lung models and Th2 cells effectively suppressed immune cell infiltration and IL-13, IL-4, TSLP, and periostin secretion, while upregulating skin barrier proteins. RNA-Seq analysis corroborate these findings and indicate protective downstream effects on the lungs. To the best of our knowledge, this represents the first report of a potent putative small molecule TSLPR inhibitor which has the potential to expand the therapeutic and preventive options in atopic diseases.
R. A. Ashraf, S. Liu, C. A. Wolf, G. Wolber, and M. Bureik. Identification of new substrates and inhibitors of human CYP2A7, Molecules, 29(10):2191, 2024.
Links:
[doi:10.3390/molecules29102191]
[show BibTeX]
[show abstract]
x
@article{RN350,
author = {Ashraf, R. A. and Liu, S. and Wolf, C. A.
and Wolber, G. and Bureik, M.},
title = {Identification of new substrates and
inhibitors of human CYP2A7},
journal = {Molecules},
volume = {29},
number = {10},
pages = {2191},
note = {Rz6p0 Times Cited:0 Cited References
Count:39},
abstract = {CYP2A7 is one of the most understudied
human cytochrome P450 enzymes and its
contributions to either drug metabolism or
endogenous biosynthesis pathways are not
understood, as its only known enzymatic
activities are the conversions of two
proluciferin probe substrates. In
addition, the CYP2A7 gene contains four
single-nucleotide polymorphisms (SNPs)
that cause missense mutations and have
minor allele frequencies (MAFs) above 0.5.
This means that the resulting amino acid
changes occur in the majority of humans.
In a previous study, we employed the
reference standard sequence (called
CYP2A7*1 in P450 nomenclature). For the
present study, we created another CYP2A7
sequence that contains all four amino acid
changes (Cys311, Glu169, Gly479, and
Arg274) and labeled it CYP2A7-WT. Thus, it
was the aim of this study to identify new
substrates and inhibitors of CYP2A7 and to
compare the properties of CYP2A7-WT with
CYP2A7*1. We found several new
proluciferin probe substrates for both
enzyme variants (we also performed in
silico studies to understand the activity
difference between CYP2A7-WT and CYP2A7*1
on specific substrates), and we show that
while they do not act on the standard
CYP2A6 substrates nicotine, coumarin, or
7-ethoxycoumarin, both can hydroxylate
diclofenac (as can CYP2A6). Moreover, we
found ketoconazole, 1-benzylimidazole, and
letrozole to be CYP2A7 inhibitors.},
DOI = {10.3390/molecules29102191},
url = {Go to ISI://WOS:001231521200001},
year = {2024},
type = {Journal Article}
}
x
Identification of new substrates and inhibitors of human CYP2A7
CYP2A7 is one of the most understudied human cytochrome P450 enzymes and its contributions to either drug metabolism or endogenous biosynthesis pathways are not understood, as its only known enzymatic activities are the conversions of two proluciferin probe substrates. In addition, the CYP2A7 gene contains four single-nucleotide polymorphisms (SNPs) that cause missense mutations and have minor allele frequencies (MAFs) above 0.5. This means that the resulting amino acid changes occur in the majority of humans. In a previous study, we employed the reference standard sequence (called CYP2A7*1 in P450 nomenclature). For the present study, we created another CYP2A7 sequence that contains all four amino acid changes (Cys311, Glu169, Gly479, and Arg274) and labeled it CYP2A7-WT. Thus, it was the aim of this study to identify new substrates and inhibitors of CYP2A7 and to compare the properties of CYP2A7-WT with CYP2A7*1. We found several new proluciferin probe substrates for both enzyme variants (we also performed in silico studies to understand the activity difference between CYP2A7-WT and CYP2A7*1 on specific substrates), and we show that while they do not act on the standard CYP2A6 substrates nicotine, coumarin, or 7-ethoxycoumarin, both can hydroxylate diclofenac (as can CYP2A6). Moreover, we found ketoconazole, 1-benzylimidazole, and letrozole to be CYP2A7 inhibitors.
U. B. A. Aziz, A. Saoud, M. Bermudez, M. Mieth, A. Atef, T. Rudolf, C. Arkona, T. Trenkner, C. Böttcher, K. Ludwig, A. Hoelzemer, A. C. Hocke, G. Wolber, and J. Rademann. Targeted small molecule inhibitors blocking the cytolytic effects of pneumolysin and homologous toxins, Nat Commun, 15(1):3537, 2024.
Links:
[doi:10.1038/s41467-024-47741-3]
[show BibTeX]
[show abstract]
x
@article{RN349,
author = {Aziz, Umer Bin Abdul and Saoud, Ali and
Bermudez, Marcel and Mieth, Maren and
Atef, Amira and Rudolf, Thomas and Arkona,
Christoph and Trenkner, Timo and
Böttcher, Christoph and Ludwig, Kai and
Hoelzemer, Angelique and Hocke, Andreas C.
and Wolber, Gerhard and Rademann, Jörg},
title = {Targeted small molecule inhibitors
blocking the cytolytic effects of
pneumolysin and homologous toxins},
journal = {Nature Communications},
volume = {15},
number = {1},
pages = {3537},
abstract = {Pneumolysin (PLY) is a
cholesterol-dependent cytolysin (CDC) from
Streptococcus pneumoniae, the main cause
for bacterial pneumonia. Liberation of PLY
during infection leads to compromised
immune system and cytolytic cell death.
Here, we report discovery, development,
and validation of targeted small molecule
inhibitors of PLY (pore-blockers, PB).
PB-1 is a virtual screening hit inhibiting
PLY-mediated hemolysis. Structural
optimization provides PB-2 with improved
efficacy. Cryo-electron tomography reveals
that PB-2 blocks PLY-binding to
cholesterol-containing membranes and
subsequent pore formation.
Scaffold-hopping delivers PB-3 with
superior chemical stability and
solubility. PB-3, formed in a
protein-templated reaction, binds to
Cys428 adjacent to the cholesterol
recognition domain of PLY with a KD of
256 nM and a residence time of 2000 s.
It acts as anti-virulence factor
preventing human lung epithelial cells
from PLY-mediated cytolysis and cell death
during infection with Streptococcus
pneumoniae and is active against the
homologous Cys-containing CDC
perfringolysin (PFO) as well.},
ISSN = {2041-1723},
DOI = {10.1038/s41467-024-47741-3},
url = {https://doi.org/10.1038/s41467-024-47741-3
https://www.nature.com/articles/s41467-024-47741-3.pdf},
year = {2024},
type = {Journal Article}
}
x
Targeted small molecule inhibitors blocking the cytolytic effects of pneumolysin and homologous toxins
Pneumolysin (PLY) is a cholesterol-dependent cytolysin (CDC) from Streptococcus pneumoniae, the main cause for bacterial pneumonia. Liberation of PLY during infection leads to compromised immune system and cytolytic cell death. Here, we report discovery, development, and validation of targeted small molecule inhibitors of PLY (pore-blockers, PB). PB-1 is a virtual screening hit inhibiting PLY-mediated hemolysis. Structural optimization provides PB-2 with improved efficacy. Cryo-electron tomography reveals that PB-2 blocks PLY-binding to cholesterol-containing membranes and subsequent pore formation. Scaffold-hopping delivers PB-3 with superior chemical stability and solubility. PB-3, formed in a protein-templated reaction, binds to Cys428 adjacent to the cholesterol recognition domain of PLY with a KD of 256 nM and a residence time of 2000 s. It acts as anti-virulence factor preventing human lung epithelial cells from PLY-mediated cytolysis and cell death during infection with Streptococcus pneumoniae and is active against the homologous Cys-containing CDC perfringolysin (PFO) as well.
N. Fuchs, L. Calvo-Barreiro, V. Talagayev, S. Pach, G. Wolber, and M. T. Gabr. From Virtual Screens to Cellular Target Engagement: New Small Molecule Ligands for the Immune Checkpoint LAG-3, ACS Medicinal Chemistry Letters, 15(11):1884-1890, 2024.
Links:
[doi:10.1021/acsmedchemlett.4c00350]
[show BibTeX]
[show abstract]
x
@article{RN354,
author = {Fuchs, Natalie and Calvo-Barreiro, Laura
and Talagayev, Valerij and Pach, Szymon
and Wolber, Gerhard and Gabr, Moustafa
T.},
title = {From Virtual Screens to Cellular Target
Engagement: New Small Molecule Ligands for
the Immune Checkpoint LAG-3},
journal = {ACS Medicinal Chemistry Letters},
volume = {15},
number = {11},
pages = {1884-1890},
note = {doi: 10.1021/acsmedchemlett.4c00350},
abstract = {Herein, we performed a virtual screening
study to discover new scaffolds for small
molecule-based ligands of the immune
checkpoint lymphocyte-activation gene 3
(LAG-3). Molecular dynamics (MD)
simulations using the LAG-3 structure
revealed two putative binding sites for
small molecules: the antibody interface
and the lipophilic canyon. A 3D
pharmacophore screening resulted in the
identification of potential ligands for
these binding sites and afforded a library
of 25 compounds. We then evaluated the
screening hits for LAG-3 binding via
microscale thermophoresis (MST) and
surface plasmon resonance (SPR). Our
biophysical screening identified two
binders with KD values in the low
micromolar range, compounds 3 (antibody
interface) and 25 (lipophilic canyon).
Furthermore, we investigated the ability
of LAG-3 hits to engage LAG-3 on a
cellular level using a cellular thermal
shift assay (CETSA). In summary, compound
3 shows potential as a lead but is not yet
a development candidate.},
DOI = {10.1021/acsmedchemlett.4c00350},
url = {https://doi.org/10.1021/acsmedchemlett.4c00350},
year = {2024},
type = {Journal Article}
}
x
From Virtual Screens to Cellular Target Engagement: New Small Molecule Ligands for the Immune Checkpoint LAG-3
Herein, we performed a virtual screening study to discover new scaffolds for small molecule-based ligands of the immune checkpoint lymphocyte-activation gene 3 (LAG-3). Molecular dynamics (MD) simulations using the LAG-3 structure revealed two putative binding sites for small molecules: the antibody interface and the lipophilic canyon. A 3D pharmacophore screening resulted in the identification of potential ligands for these binding sites and afforded a library of 25 compounds. We then evaluated the screening hits for LAG-3 binding via microscale thermophoresis (MST) and surface plasmon resonance (SPR). Our biophysical screening identified two binders with KD values in the low micromolar range, compounds 3 (antibody interface) and 25 (lipophilic canyon). Furthermore, we investigated the ability of LAG-3 hits to engage LAG-3 on a cellular level using a cellular thermal shift assay (CETSA). In summary, compound 3 shows potential as a lead but is not yet a development candidate.
E. Koçak Aslan, K. Lam, C. Dengiz, K. Denzinger, I. Y. Dicle Erdamar, S. Huang, G. W. Zamponi, G. Wolber, and M. G. Gunduz. Synthesis, molecular modeling, DFT studies, and EPR analysis of 1,4-dihydropyridines as potential calcium channel blockers, J Mol Struct, 1307:137983, 2024.
Links:
[doi:10.1016/j.molstruc.2024.137983]
[show BibTeX]
[show abstract]
x
@article{RN348,
author = {Koçak Aslan, Ebru and Lam, Kevin and
Dengiz, Cagatay and Denzinger, Katrin and
Dicle Erdamar, Isık Yesim and Huang, Sun
and Zamponi, Gerald W. and Wolber, Gerhard
and Gunduz, Miyase Gozde},
title = {Synthesis, molecular modeling, DFT
studies, and EPR analysis of
1,4-dihydropyridines as potential calcium
channel blockers},
journal = {Journal of Molecular Structure},
volume = {1307},
pages = {137983},
abstract = {1,4-Dihydropyridines (DHPs) are widely
recognized as a highly effective class of
L-type calcium channel blockers that offer
significant therapeutic potential in
managing cardiovascular conditions.
Furthermore, their ability to target other
types of calcium channels makes DHPs
attractive candidates for therapeutic
applications in neurological and
psychiatric disorders. Close examination
of the chemical structures of approved
DHP-based antihypertensive drugs with a
history of over forty years in the market
reveals that the C-4 position is the least
altered part of this privileged ring
system. In the present study, we focused
on this position and synthesized two novel
compounds (DB1 and DB2) by carrying out
chemical modifications on suitable
positions of the main scaffold of DA1
(isobutyl
4-(benzo[d][1,3]dioxol-5-yl)-2,6,6-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate)
that was previously identified as a
DHP-based effective and selective
inhibitor of T-type (Cav3.2) over L-type
(Cav1.2) calcium channel. Based on
whole-cell patch-clamp analysis on Cav1.2
and Cav3.2, DB1 with bromine group on the
benzodioxole ring appeared to be a more
effective and selective inhibitor of
Cav3.2 compared to DB2 with nitro at the
same locus. Molecular docking and
molecular dynamics (MD) simulations were
performed to investigate the binding mode
of both DB1 isomers to Cav3.2.
Furthermore, density functional theory
(DFT) methods were employed to obtain
information regarding the stability of the
molecules by computing various parameters,
such as electric dipole moment, band gap,
electronegativity, and global chemical
hardness-softness, related to their
charge-transfer characteristics. According
to DFT studies, DB1 also appeared to be
chemically more stable than DB2. Finally,
ionizing radiation-induced free radicals
of gamma-irradiated DB1 and DB2 in powder
form were examined utilizing the electron
paramagnetic resonance (EPR) technique and
the obtained data demonstrated that
radiation sterilization is suitable for
the dosage forms including DB1 and DB2.},
ISSN = {0022-2860},
DOI = {10.1016/j.molstruc.2024.137983},
url = {https://www.sciencedirect.com/science/article/pii/S0022286024005052
https://www.sciencedirect.com/science/article/pii/S0022286024005052?via%3Dihub},
year = {2024},
type = {Journal Article}
}
x
Synthesis, molecular modeling, DFT studies, and EPR analysis of 1,4-dihydropyridines as potential calcium channel blockers
1,4-Dihydropyridines (DHPs) are widely recognized as a highly effective class of L-type calcium channel blockers that offer significant therapeutic potential in managing cardiovascular conditions. Furthermore, their ability to target other types of calcium channels makes DHPs attractive candidates for therapeutic applications in neurological and psychiatric disorders. Close examination of the chemical structures of approved DHP-based antihypertensive drugs with a history of over forty years in the market reveals that the C-4 position is the least altered part of this privileged ring system. In the present study, we focused on this position and synthesized two novel compounds (DB1 and DB2) by carrying out chemical modifications on suitable positions of the main scaffold of DA1 (isobutyl 4-(benzo[d][1,3]dioxol-5-yl)-2,6,6-trimethyl-5-oxo-1,4,5,6,7,8-hexahydroquinoline-3-carboxylate) that was previously identified as a DHP-based effective and selective inhibitor of T-type (Cav3.2) over L-type (Cav1.2) calcium channel. Based on whole-cell patch-clamp analysis on Cav1.2 and Cav3.2, DB1 with bromine group on the benzodioxole ring appeared to be a more effective and selective inhibitor of Cav3.2 compared to DB2 with nitro at the same locus. Molecular docking and molecular dynamics (MD) simulations were performed to investigate the binding mode of both DB1 isomers to Cav3.2. Furthermore, density functional theory (DFT) methods were employed to obtain information regarding the stability of the molecules by computing various parameters, such as electric dipole moment, band gap, electronegativity, and global chemical hardness-softness, related to their charge-transfer characteristics. According to DFT studies, DB1 also appeared to be chemically more stable than DB2. Finally, ionizing radiation-induced free radicals of gamma-irradiated DB1 and DB2 in powder form were examined utilizing the electron paramagnetic resonance (EPR) technique and the obtained data demonstrated that radiation sterilization is suitable for the dosage forms including DB1 and DB2.
F. Li, S. Ackloo, C. H. Arrowsmith, F. Ban, C. J. Barden, H. Beck, J. Beránek, F. Berenger, A. Bolotokova, G. Bret, M. Breznik, E. Carosati, I. Chau, Y. Chen, A. Cherkasov, D. D. Corte, K. Denzinger, A. Dong, S. Draga, I. Dunn, K. Edfeldt, A. Edwards, M. Eguida, P. Eisenhuth, L. Friedrich, A. Fuerll, S. S. Gardiner, F. Gentile, P. Ghiabi, E. Gibson, M. Glavatskikh, C. Gorgulla, J. Guenther, A. Gunnarsson, F. Gusev, E. Gutkin, L. Halabelian, R. J. Harding, A. Hillisch, L. Hoffer, A. Hogner, S. Houliston, J. J. Irwin, O. Isayev, A. Ivanova, C. Jacquemard, A. J. Jarrett, J. H. Jensen, D. Kireev, J. Kleber, S. B. Koby, D. Koes, A. Kumar, M. G. Kurnikova, A. Kutlushina, U. Lessel, F. Liessmann, S. Liu, W. Lu, J. Meiler, A. Mettu, G. Minibaeva, R. Moretti, C. J. Morris, C. Narangoda, T. Noonan, L. Obendorf, S. Pach, A. Pandit, S. Perveen, G. Poda, P. Polishchuk, K. Puls, V. Pütter, D. Rognan, D. Roskams-Edris, C. Schindler, F. Sindt, V. Spiwok, C. Steinmann, R. L. Stevens, V. Talagayev, D. Tingey, O. Vu, W. P. Walters, X. Wang, Z. Wang, G. Wolber, C. A. Wolf, L. Wortmann, H. Zeng, C. A. Zepeda, K. Y. J. Zhang, J. Zhang, S. Zheng, and M. Schapira. CACHE Challenge #1: Targeting the WDR Domain of LRRK2, A Parkinson’s Disease Associated Protein, J Chem Inf Model, 64(22):8521-8536, 2024.
Links:
[doi:10.1021/acs.jcim.4c01267]
[show BibTeX]
[show abstract]
x
@article{RN352,
author = {Li, Fengling and Ackloo, Suzanne and
Arrowsmith, Cheryl H. and Ban, Fuqiang and
Barden, Christopher J. and Beck, Hartmut
and Beránek, Jan and Berenger, Francois
and Bolotokova, Albina and Bret, Guillaume
and Breznik, Marko and Carosati, Emanuele
and Chau, Irene and Chen, Yu and
Cherkasov, Artem and Corte, Dennis Della
and Denzinger, Katrin and Dong, Aiping and
Draga, Sorin and Dunn, Ian and Edfeldt,
Kristina and Edwards, Aled and Eguida,
Merveille and Eisenhuth, Paul and
Friedrich, Lukas and Fuerll, Alexander and
Gardiner, Spencer S. and Gentile,
Francesco and Ghiabi, Pegah and Gibson,
Elisa and Glavatskikh, Marta and Gorgulla,
Christoph and Guenther, Judith and
Gunnarsson, Anders and Gusev, Filipp and
Gutkin, Evgeny and Halabelian, Levon and
Harding, Rachel J. and Hillisch, Alexander
and Hoffer, Laurent and Hogner, Anders and
Houliston, Scott and Irwin, John J. and
Isayev, Olexandr and Ivanova, Aleksandra
and Jacquemard, Celien and Jarrett, Austin
J. and Jensen, Jan H. and Kireev, Dmitri
and Kleber, Julian and Koby, S. Benjamin
and Koes, David and Kumar, Ashutosh and
Kurnikova, Maria G. and Kutlushina, Alina
and Lessel, Uta and Liessmann, Fabian and
Liu, Sijie and Lu, Wei and Meiler, Jens
and Mettu, Akhila and Minibaeva, Guzel and
Moretti, Rocco and Morris, Connor J. and
Narangoda, Chamali and Noonan, Theresa and
Obendorf, Leon and Pach, Szymon and
Pandit, Amit and Perveen, Sumera and Poda,
Gennady and Polishchuk, Pavel and Puls,
Kristina and Pütter, Vera and Rognan,
Didier and Roskams-Edris, Dylan and
Schindler, Christina and Sindt, François
and Spiwok, Vojtěch and Steinmann, Casper
and Stevens, Rick L. and Talagayev,
Valerij and Tingey, Damon and Vu, Oanh and
Walters, W. Patrick and Wang, Xiaowen and
Wang, Zhenyu and Wolber, Gerhard and Wolf,
Clemens Alexander and Wortmann, Lars and
Zeng, Hong and Zepeda, Carlos A. and
Zhang, Kam Y. J. and Zhang, Jixian and
Zheng, Shuangjia and Schapira, Matthieu},
title = {CACHE Challenge #1: Targeting the WDR
Domain of LRRK2, A Parkinson’s Disease
Associated Protein},
journal = {Journal of Chemical Information and
Modeling},
volume = {64},
number = {22},
pages = {8521-8536},
note = {doi: 10.1021/acs.jcim.4c01267},
abstract = {The CACHE challenges are a series of
prospective benchmarking exercises to
evaluate progress in the field of
computational hit-finding. Here we report
the results of the inaugural CACHE
challenge in which 23 computational teams
each selected up to 100 commercially
available compounds that they predicted
would bind to the WDR domain of the
Parkinson’s disease target LRRK2, a
domain with no known ligand and only an
apo structure in the PDB. The lack of
known binding data and presumably low
druggability of the target is a challenge
to computational hit finding methods. Of
the 1955 molecules predicted by
participants in Round 1 of the challenge,
73 were found to bind to LRRK2 in an SPR
assay with a KD lower than 150 μM. These
73 molecules were advanced to the Round 2
hit expansion phase, where computational
teams each selected up to 50 analogs.
Binding was observed in two orthogonal
assays for seven chemically diverse
series, with affinities ranging from 18 to
140 μM. The seven successful
computational workflows varied in their
screening strategies and techniques. Three
used molecular dynamics to produce a
conformational ensemble of the targeted
site, three included a fragment docking
step, three implemented a generative
design strategy and five used one or more
deep learning steps. CACHE #1 reflects a
highly exploratory phase in computational
drug design where participants adopted
strikingly diverging screening strategies.
Machine learning-accelerated methods
achieved similar results to brute force
(e.g., exhaustive) docking.
First-in-class, experimentally confirmed
compounds were rare and weakly potent,
indicating that recent advances are not
sufficient to effectively address
challenging targets.},
ISSN = {1549-9596},
DOI = {10.1021/acs.jcim.4c01267},
url = {https://doi.org/10.1021/acs.jcim.4c01267},
year = {2024},
type = {Journal Article}
}
x
CACHE Challenge #1: Targeting the WDR Domain of LRRK2, A Parkinson’s Disease Associated Protein
The CACHE challenges are a series of prospective benchmarking exercises to evaluate progress in the field of computational hit-finding. Here we report the results of the inaugural CACHE challenge in which 23 computational teams each selected up to 100 commercially available compounds that they predicted would bind to the WDR domain of the Parkinson’s disease target LRRK2, a domain with no known ligand and only an apo structure in the PDB. The lack of known binding data and presumably low druggability of the target is a challenge to computational hit finding methods. Of the 1955 molecules predicted by participants in Round 1 of the challenge, 73 were found to bind to LRRK2 in an SPR assay with a KD lower than 150 μM. These 73 molecules were advanced to the Round 2 hit expansion phase, where computational teams each selected up to 50 analogs. Binding was observed in two orthogonal assays for seven chemically diverse series, with affinities ranging from 18 to 140 μM. The seven successful computational workflows varied in their screening strategies and techniques. Three used molecular dynamics to produce a conformational ensemble of the targeted site, three included a fragment docking step, three implemented a generative design strategy and five used one or more deep learning steps. CACHE #1 reflects a highly exploratory phase in computational drug design where participants adopted strikingly diverging screening strategies. Machine learning-accelerated methods achieved similar results to brute force (e.g., exhaustive) docking. First-in-class, experimentally confirmed compounds were rare and weakly potent, indicating that recent advances are not sufficient to effectively address challenging targets.
K. Puls, A. Olivé-Marti, S. Hongnak, D. Lamp, M. Spetea, and G. Wolber. Discovery of Novel, Selective, and Nonbasic Agonists for the Kappa-Opioid Receptor Determined by Salvinorin A-Based Virtual Screening, J Med Chem, 67(16):13788-13801, 2024.
Links:
[doi:10.1021/acs.jmedchem.4c00590]
[show BibTeX]
[show abstract]
x
@article{RN355,
author = {Puls, Kristina and Olivé-Marti,
Aina-Leonor and Hongnak, Siriwat and Lamp,
David and Spetea, Mariana and Wolber,
Gerhard},
title = {Discovery of Novel, Selective, and
Nonbasic Agonists for the Kappa-Opioid
Receptor Determined by Salvinorin A-Based
Virtual Screening},
journal = {Journal of Medicinal Chemistry},
volume = {67},
number = {16},
pages = {13788-13801},
note = {doi: 10.1021/acs.jmedchem.4c00590},
abstract = {Modulating the kappa-opioid receptor
(KOR) is a promising strategy for treating
various human diseases. KOR agonists show
potential for treating pain, pruritus, and
epilepsy, while KOR antagonists show
potential for treating depression,
anxiety, and addiction. The diterpenoid
Salvinorin A (SalA), a secondary
metabolite of Salvia divinorum, is a
potent and selective KOR agonist. Unlike
typical opioids, SalA lacks a basic
nitrogen, which encouraged us to search
for nonbasic KOR ligands. Through
structure-based virtual screening using 3D
pharmacophore models based on the binding
mode of SalA, we identified novel,
nonbasic, potent, and selective KOR
agonists. In vitro studies confirmed two
virtual hits, SalA-VS-07 and SalA-VS-08,
as highly selective for the KOR and
showing G protein-biased KOR agonist
activity. Both KOR ligands share a novel
spiro-moiety and a nonbasic scaffold. Our
findings provide novel starting points for
developing therapeutics aimed at treating
pain and other conditions in which KOR is
a central player.},
ISSN = {0022-2623},
DOI = {10.1021/acs.jmedchem.4c00590},
url = {https://doi.org/10.1021/acs.jmedchem.4c00590
https://pmc.ncbi.nlm.nih.gov/articles/PMC11345774/pdf/jm4c00590.pdf},
year = {2024},
type = {Journal Article}
}
x
Discovery of Novel, Selective, and Nonbasic Agonists for the Kappa-Opioid Receptor Determined by Salvinorin A-Based Virtual Screening
Modulating the kappa-opioid receptor (KOR) is a promising strategy for treating various human diseases. KOR agonists show potential for treating pain, pruritus, and epilepsy, while KOR antagonists show potential for treating depression, anxiety, and addiction. The diterpenoid Salvinorin A (SalA), a secondary metabolite of Salvia divinorum, is a potent and selective KOR agonist. Unlike typical opioids, SalA lacks a basic nitrogen, which encouraged us to search for nonbasic KOR ligands. Through structure-based virtual screening using 3D pharmacophore models based on the binding mode of SalA, we identified novel, nonbasic, potent, and selective KOR agonists. In vitro studies confirmed two virtual hits, SalA-VS-07 and SalA-VS-08, as highly selective for the KOR and showing G protein-biased KOR agonist activity. Both KOR ligands share a novel spiro-moiety and a nonbasic scaffold. Our findings provide novel starting points for developing therapeutics aimed at treating pain and other conditions in which KOR is a central player.
L. L. Sallandt, C. A. Wolf, S. Schuster, H. Enke, D. Enke, G. Wolber, and T. H. J. Niedermeyer. Derivatization of Microcystins Can Increase Target Inhibition while Reducing Cellular Uptake, J Nat Prod, :e-pub ahead of print, 2024.
Links:
[doi:10.1021/acs.jnatprod.4c00688]
[show BibTeX]
[show abstract]
x
@article{RN353,
author = {Sallandt, Laura L. and Wolf, Clemens A.
and Schuster, Sabine and Enke, Heike and
Enke, Dan and Wolber, Gerhard and
Niedermeyer, Timo H. J.},
title = {Derivatization of Microcystins Can
Increase Target Inhibition while Reducing
Cellular Uptake},
journal = {Journal of Natural Products},
pages = {e-pub ahead of print},
note = {doi: 10.1021/acs.jnatprod.4c00688},
abstract = {Microcystins, a large family of
nonribosomal cyclic heptapeptides known
for their hepatotoxicity, are among the
best-studied cyanobacterial toxins.
Recently, they have been discussed as
leads for the development of anticancer
drug substances. Their main mode-of-action
is inhibition of the eukaryotic
serine/threonine protein phosphatases 1
and 2A. Unlike many cytotoxins that can
cross cell membranes by passive diffusion,
microcystins depend on active uptake via
organic anion transporting polypeptides
1B1 or 1B3. Both phosphatase inhibition
and transportability strongly depend on
the structure of the individual
microcystin. Here, we present how chemical
modification of positions 2 and 4 of the
microcystin core structure can alter these
two properties. Aiming to reduce
transportability and increase phosphatase
inhibition, we used pharmacophore modeling
to investigate the phosphatase inhibition
potential of microcystins derivatized with
small molecules containing a variety of
functional groups. The respective
derivatives were synthesized using click
chemistry. We discovered that some
derivatized microcystins can address a yet
undescribed subpocket of the protein
phosphatase 1. The derivatized
microcystins were tested for phosphatase 1
inhibition and cytotoxicity on
transporter-expressing cell lines,
revealing that target inhibition and
transportability of microcystins can
independently be influenced by the
physicochemical properties, especially of
the residue located in position 2 of the
microcystin. Derivatization with small
acids or amino acids resulted in
microcystins with a favorable ratio of
inhibition to transportability, making
these derivatives potentially suitable for
drug development.},
ISSN = {0163-3864},
DOI = {10.1021/acs.jnatprod.4c00688},
url = {https://doi.org/10.1021/acs.jnatprod.4c00688},
year = {2024},
type = {Journal Article}
}
x
Derivatization of Microcystins Can Increase Target Inhibition while Reducing Cellular Uptake
Microcystins, a large family of nonribosomal cyclic heptapeptides known for their hepatotoxicity, are among the best-studied cyanobacterial toxins. Recently, they have been discussed as leads for the development of anticancer drug substances. Their main mode-of-action is inhibition of the eukaryotic serine/threonine protein phosphatases 1 and 2A. Unlike many cytotoxins that can cross cell membranes by passive diffusion, microcystins depend on active uptake via organic anion transporting polypeptides 1B1 or 1B3. Both phosphatase inhibition and transportability strongly depend on the structure of the individual microcystin. Here, we present how chemical modification of positions 2 and 4 of the microcystin core structure can alter these two properties. Aiming to reduce transportability and increase phosphatase inhibition, we used pharmacophore modeling to investigate the phosphatase inhibition potential of microcystins derivatized with small molecules containing a variety of functional groups. The respective derivatives were synthesized using click chemistry. We discovered that some derivatized microcystins can address a yet undescribed subpocket of the protein phosphatase 1. The derivatized microcystins were tested for phosphatase 1 inhibition and cytotoxicity on transporter-expressing cell lines, revealing that target inhibition and transportability of microcystins can independently be influenced by the physicochemical properties, especially of the residue located in position 2 of the microcystin. Derivatization with small acids or amino acids resulted in microcystins with a favorable ratio of inhibition to transportability, making these derivatives potentially suitable for drug development.
2023
[198]
S. A. Abdel-Rahman, V. Talagayev, S. Pach, G. Wolber, and M. T. Gabr. Discovery of Small-Molecule TIM-3 Inhibitors for Acute Myeloid Leukemia Using Pharmacophore-Based Virtual Screening, J Med Chem, 66(16):11464-11475, 2023.
Links:
[doi:10.1021/acs.jmedchem.3c00960]
[show BibTeX]
[show abstract]
x
@article{RN339,
author = {Abdel-Rahman, Somaya A. and Talagayev,
Valerij and Pach, Szymon and Wolber,
Gerhard and Gabr, Moustafa T.},
title = {Discovery of Small-Molecule TIM-3
Inhibitors for Acute Myeloid Leukemia
Using Pharmacophore-Based Virtual
Screening},
journal = {Journal of Medicinal Chemistry},
volume = {66},
number = {16},
pages = {11464-11475},
note = {doi: 10.1021/acs.jmedchem.3c00960},
abstract = {T-cell immunoglobulin and mucin domain 3
(TIM-3) is a negative immune checkpoint
that represents a promising target for
cancer immunotherapy. Although encouraging
results have been observed for TIM-3
inhibition in the context of acute myeloid
leukemia (AML), targeting TIM-3 is
currently restricted to monoclonal
antibodies (mAbs). To fill this gap, we
implemented a pharmacophore-based
screening approach to identify
small-molecule TIM-3 inhibitors. Our
approach resulted in the identification of
hit compounds with TIM-3 binding affinity.
Subsequently, we used the
structure–activity relationship (SAR) by
a catalog approach to identify compound
A-41 with submicromolar TIM-3 binding
affinity. Remarkably, A-41 demonstrated
the ability to block TIM-3 interactions
with key ligands and inhibited the
immunosuppressive function of TIM-3 using
an in vitro coculture assay. This work
will pave the way for future drug
discovery efforts aiming at the
development of small-molecule inhibitors
TIM-3 for AML.},
ISSN = {0022-2623},
DOI = {10.1021/acs.jmedchem.3c00960},
url = {https://doi.org/10.1021/acs.jmedchem.3c00960},
year = {2023},
type = {Journal Article}
}
x
Discovery of Small-Molecule TIM-3 Inhibitors for Acute Myeloid Leukemia Using Pharmacophore-Based Virtual Screening
T-cell immunoglobulin and mucin domain 3 (TIM-3) is a negative immune checkpoint that represents a promising target for cancer immunotherapy. Although encouraging results have been observed for TIM-3 inhibition in the context of acute myeloid leukemia (AML), targeting TIM-3 is currently restricted to monoclonal antibodies (mAbs). To fill this gap, we implemented a pharmacophore-based screening approach to identify small-molecule TIM-3 inhibitors. Our approach resulted in the identification of hit compounds with TIM-3 binding affinity. Subsequently, we used the structure–activity relationship (SAR) by a catalog approach to identify compound A-41 with submicromolar TIM-3 binding affinity. Remarkably, A-41 demonstrated the ability to block TIM-3 interactions with key ligands and inhibited the immunosuppressive function of TIM-3 using an in vitro coculture assay. This work will pave the way for future drug discovery efforts aiming at the development of small-molecule inhibitors TIM-3 for AML.
L. Calvo-Barreiro, V. Talagayev, S. Pach, S. A. Abdel-Rahman, G. Wolber, and M. T. Gabr. Discovery of ICOS-Targeted Small Molecules Using Pharmacophore-Based Screening, ChemMedChem, n/a(n/a):e202300305, 2023.
Links:
[doi:https://.org/10.1002/cmdc.202300305]
[show BibTeX]
[show abstract]
x
@article{RN343,
author = {Calvo-Barreiro, Laura and Talagayev,
Valerij and Pach, Szymon and Abdel-Rahman,
Somaya A. and Wolber, Gerhard and Gabr,
Moustafa T.},
title = {Discovery of ICOS-Targeted Small
Molecules Using Pharmacophore-Based
Screening},
journal = {ChemMedChem},
volume = {n/a},
number = {n/a},
pages = {e202300305},
abstract = {Abstract There are currently no small
molecules clinically approved as immune
checkpoint modulators. Besides possessing
oral bioavailability, cell-penetrating
capabilities and enhanced tumor
penetration compared to monoclonal
antibodies (mAbs), small molecules are
amenable to pharmacokinetic optimization,
which allows adopting flexible dosage
regimens that may avoid immune-related
adverse events associated with mAbs. The
interaction of inducible co-stimulator
(ICOS) with its ligand (ICOS-L) plays key
roles in T-cell differentiation and
activation of T-cell to B-cell functions.
This study represents the development and
validation of a virtual screening strategy
to identify small molecules that bind a
novel druggable binding pocket in human
ICOS. We used a lipophilic canyon in the
apo-structure of ICOS and the ICOS/ICOS-L
interface individually as templates for
molecular dynamics simulation to generate
3D pharmacophores subsequently used for
virtual screening campaigns. Our strategy
was successful finding a first-in-class
small molecule ICOS binder (5P, KD
value=108.08±26.76??M) and validating
biophysical screening platforms for
ICOS-targeted small molecules. We
anticipate that future structural
optimization of 5P will result in the
discovery of high affinity chemical
ligands for ICOS.},
ISSN = {1860-7179},
DOI = {https://doi.org/10.1002/cmdc.202300305},
url = {https://doi.org/10.1002/cmdc.202300305
https://chemistry-europe.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cmdc.202300305?download= true},
year = {2023},
type = {Journal Article}
}
x
Discovery of ICOS-Targeted Small Molecules Using Pharmacophore-Based Screening
Abstract There are currently no small molecules clinically approved as immune checkpoint modulators. Besides possessing oral bioavailability, cell-penetrating capabilities and enhanced tumor penetration compared to monoclonal antibodies (mAbs), small molecules are amenable to pharmacokinetic optimization, which allows adopting flexible dosage regimens that may avoid immune-related adverse events associated with mAbs. The interaction of inducible co-stimulator (ICOS) with its ligand (ICOS-L) plays key roles in T-cell differentiation and activation of T-cell to B-cell functions. This study represents the development and validation of a virtual screening strategy to identify small molecules that bind a novel druggable binding pocket in human ICOS. We used a lipophilic canyon in the apo-structure of ICOS and the ICOS/ICOS-L interface individually as templates for molecular dynamics simulation to generate 3D pharmacophores subsequently used for virtual screening campaigns. Our strategy was successful finding a first-in-class small molecule ICOS binder (5P, KD value=108.08±26.76??M) and validating biophysical screening platforms for ICOS-targeted small molecules. We anticipate that future structural optimization of 5P will result in the discovery of high affinity chemical ligands for ICOS.
Y. Jia, B. Schroeder, Y. Pfeifer, C. Fröhlich, L. Deng, C. Arkona, B. Kuropka, J. Sticht, K. Ataka, S. Bergemann, G. Wolber, C. Nitsche, M. Mielke, H. S. Leiros, G. Werner, and J. Rademann. Kinetics, Thermodynamics, and Structural Effects of Quinoline-2-Carboxylates, Zinc-Binding Inhibitors of New Delhi Metallo-β-lactamase-1 Re-sensitizing Multidrug-Resistant Bacteria for Carbapenems, J Med Chem, 66(17):11761-11791, 2023.
Links:
[doi:10.1021/acs.jmedchem.3c00171]
[show BibTeX]
[show abstract]
x
@article{RN337,
author = {Jia, Yuwen and Schroeder, Barbara and
Pfeifer, Yvonne and Fröhlich, Christopher
and Deng, Lihua and Arkona, Christoph and
Kuropka, Benno and Sticht, Jana and Ataka,
Kenichi and Bergemann, Silke and Wolber,
Gerhard and Nitsche, Christoph and Mielke,
Martin and Leiros, Hanna-Kirsti S. and
Werner, Guido and Rademann, Jörg},
title = {Kinetics, Thermodynamics, and Structural
Effects of Quinoline-2-Carboxylates,
Zinc-Binding Inhibitors of New Delhi
Metallo-β-lactamase-1 Re-sensitizing
Multidrug-Resistant Bacteria for
Carbapenems},
journal = {Journal of Medicinal Chemistry},
volume = {66},
number = {17},
pages = {11761-11791},
note = {doi: 10.1021/acs.jmedchem.3c00171},
abstract = {Carbapenem resistance mediated by
metallo-β-lactamases (MBL) such as New
Delhi metallo-β-lactamase-1 (NDM-1) has
become a major factor threatening the
efficacy of essential β-lactam
antibiotics. Starting from hit fragment
dipicolinic acid (DPA), 8-hydroxy- and
8-sulfonamido-quinoline-2-carboxylic acids
were developed as inhibitors of NDM-1 with
highly improved inhibitory activity and
binding affinity. The most active
compounds formed reversibly inactive
ternary protein-inhibitor complexes with
two zinc ions as proven by native protein
mass spectrometry and bio-layer
interferometry. Modification of the NDM-1
structure with remarkable entropic gain
was shown by isothermal titration
calorimetry and NMR spectroscopy of
isotopically labeled protein. The best
compounds were potent inhibitors of NDM-1
and other representative MBL with no or
little inhibition of human zinc-binding
enzymes. These inhibitors significantly
reduced the minimum inhibitory
concentrations (MIC) of meropenem for
multidrug-resistant bacteria recombinantly
expressing blaNDM-1 as well as for several
multidrug-resistant clinical strains at
concentrations non-toxic to human cells.},
ISSN = {0022-2623},
DOI = {10.1021/acs.jmedchem.3c00171},
url = {https://doi.org/10.1021/acs.jmedchem.3c00171},
year = {2023},
type = {Journal Article}
}
x
Kinetics, Thermodynamics, and Structural Effects of Quinoline-2-Carboxylates, Zinc-Binding Inhibitors of New Delhi Metallo-β-lactamase-1 Re-sensitizing Multidrug-Resistant Bacteria for Carbapenems
Carbapenem resistance mediated by metallo-β-lactamases (MBL) such as New Delhi metallo-β-lactamase-1 (NDM-1) has become a major factor threatening the efficacy of essential β-lactam antibiotics. Starting from hit fragment dipicolinic acid (DPA), 8-hydroxy- and 8-sulfonamido-quinoline-2-carboxylic acids were developed as inhibitors of NDM-1 with highly improved inhibitory activity and binding affinity. The most active compounds formed reversibly inactive ternary protein-inhibitor complexes with two zinc ions as proven by native protein mass spectrometry and bio-layer interferometry. Modification of the NDM-1 structure with remarkable entropic gain was shown by isothermal titration calorimetry and NMR spectroscopy of isotopically labeled protein. The best compounds were potent inhibitors of NDM-1 and other representative MBL with no or little inhibition of human zinc-binding enzymes. These inhibitors significantly reduced the minimum inhibitory concentrations (MIC) of meropenem for multidrug-resistant bacteria recombinantly expressing blaNDM-1 as well as for several multidrug-resistant clinical strains at concentrations non-toxic to human cells.
V. Kremling, B. Loll, S. Pach, I. Dahmani, C. Weise, G. Wolber, S. Chiantia, M. C. Wahl, N. Osterrieder, and W. Azab. Crystal structures of glycoprotein D of equine alphaherpesviruses reveal potential binding sites to the entry receptor MHC-I, Frontiers in Microbiology, 14:1197120, 2023.
Links:
[doi:10.3389/fmicb.2023.1197120]
[show BibTeX]
[show abstract]
x
@article{RN335,
author = {Kremling, Viviane and Loll, Bernhard and
Pach, Szymon and Dahmani, Ismail and
Weise, Christoph and Wolber, Gerhard and
Chiantia, Salvatore and Wahl, Markus C.
and Osterrieder, Nikolaus and Azab,
Walid},
title = {Crystal structures of glycoprotein D of
equine alphaherpesviruses reveal potential
binding sites to the entry receptor
MHC-I},
journal = {Frontiers in Microbiology},
volume = {14},
pages = {1197120},
abstract = {Cell entry of most alphaherpesviruses is
mediated by the binding of glycoprotein D
(gD) to different cell surface receptors.
Equine herpesvirus type 1 (EHV-1) and
EHV-4 gDs interact with equine major
histocompatibility complex I (MHC-I) to
initiate entry into equine cells. We have
characterized the gD-MHC-I interaction by
solving the crystal structures of EHV-1
and EHV-4 gDs (gD1, gD4), performing
protein-protein docking simulations,
surface plasmon resonance (SPR) analysis,
and biological assays. The structures of
gD1 and gD4 revealed the existence of a
common V-set immunoglobulin-like
(IgV-like) core comparable to those of
other gD homologs. Molecular modeling
yielded plausible binding hypotheses and
identified key residues (F213 and D261)
that are important for virus binding.
Altering the key residues resulted in
impaired virus growth in cells, which
highlights the important role of these
residues in the gD-MHC-I interaction.
Taken together, our results add to our
understanding of the initial
herpesvirus-cell interactions and will
contribute to the targeted design of
antiviral drugs and vaccine development.},
ISSN = {1664-302X},
DOI = {10.3389/fmicb.2023.1197120},
url = {https://www.frontiersin.org/articles/10.3389/fmicb.2023.1197120},
year = {2023},
type = {Journal Article}
}
x
Crystal structures of glycoprotein D of equine alphaherpesviruses reveal potential binding sites to the entry receptor MHC-I
Cell entry of most alphaherpesviruses is mediated by the binding of glycoprotein D (gD) to different cell surface receptors. Equine herpesvirus type 1 (EHV-1) and EHV-4 gDs interact with equine major histocompatibility complex I (MHC-I) to initiate entry into equine cells. We have characterized the gD-MHC-I interaction by solving the crystal structures of EHV-1 and EHV-4 gDs (gD1, gD4), performing protein-protein docking simulations, surface plasmon resonance (SPR) analysis, and biological assays. The structures of gD1 and gD4 revealed the existence of a common V-set immunoglobulin-like (IgV-like) core comparable to those of other gD homologs. Molecular modeling yielded plausible binding hypotheses and identified key residues (F213 and D261) that are important for virus binding. Altering the key residues resulted in impaired virus growth in cells, which highlights the important role of these residues in the gD-MHC-I interaction. Taken together, our results add to our understanding of the initial herpesvirus-cell interactions and will contribute to the targeted design of antiviral drugs and vaccine development.
R. Maccari, G. Wolber, M. Genovese, G. Sardelli, V. Talagayev, F. Balestri, S. Luti, A. Santi, R. Moschini, A. Del Corso, P. Paoli, and R. Ottana. Designed multiple ligands for the treatment of type 2 diabetes mellitus and its complications: Discovery of (5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic acids active as novel dual-targeted PTP1B/AKR1B1 inhibitors, Eur J Med Chem, 252:115270, 2023.
Links:
[doi:10.1016/j.ejmech.2023.115270]
[show BibTeX]
[show abstract]
x
@article{RN334,
author = {Maccari, Rosanna and Wolber, Gerhard and
Genovese, Massimo and Sardelli, Gemma and
Talagayev, Valerij and Balestri, Francesco
and Luti, Simone and Santi, Alice and
Moschini, Roberta and Del Corso, Antonella
and Paoli, Paolo and Ottana, Rosaria},
title = {Designed multiple ligands for the
treatment of type 2 diabetes mellitus and
its complications: Discovery of
(5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic
acids active as novel dual-targeted
PTP1B/AKR1B1 inhibitors},
journal = {European Journal of Medicinal Chemistry},
volume = {252},
pages = {115270},
abstract = {Type 2 diabetes mellitus (T2DM) is a
serious chronic disease with an alarmingly
growing worldwide prevalence. Current
treatment of T2DM mainly relies on drug
combinations in order to control blood
glucose levels and consequently prevent
the onset of hyperglycaemia-related
complications. The development of
multiple-targeted drugs recently emerged
as an attractive alternative to drug
combinations for the treatment of complex
diseases with multifactorial pathogenesis,
such as T2DM. Protein tyrosine phosphatase
1B (PTP1B) and aldose reductase (AKR1B1)
are two enzymes crucially involved in the
development of T2DM and its chronic
complications and, therefore, dual
inhibitors targeted to both these enzymes
could provide novel agents for the
treatment of this complex pathological
condition. In continuing our search for
dual-targeted PTP1B/AKR1B1 inhibitors, we
designed new
(5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic
acids. Among them,
3-(4-phenylbutoxy)benzylidene derivatives
6f and 7f, endowed with interesting
inhibitory activity against both targets,
proved to control specific cellular
pathways implicated in the development of
T2DM and related complications.},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2023.115270},
url = {https://www.sciencedirect.com/science/article/pii/S0223523423002362},
year = {2023},
type = {Journal Article}
}
x
Designed multiple ligands for the treatment of type 2 diabetes mellitus and its complications: Discovery of (5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic acids active as novel dual-targeted PTP1B/AKR1B1 inhibitors
Type 2 diabetes mellitus (T2DM) is a serious chronic disease with an alarmingly growing worldwide prevalence. Current treatment of T2DM mainly relies on drug combinations in order to control blood glucose levels and consequently prevent the onset of hyperglycaemia-related complications. The development of multiple-targeted drugs recently emerged as an attractive alternative to drug combinations for the treatment of complex diseases with multifactorial pathogenesis, such as T2DM. Protein tyrosine phosphatase 1B (PTP1B) and aldose reductase (AKR1B1) are two enzymes crucially involved in the development of T2DM and its chronic complications and, therefore, dual inhibitors targeted to both these enzymes could provide novel agents for the treatment of this complex pathological condition. In continuing our search for dual-targeted PTP1B/AKR1B1 inhibitors, we designed new (5-arylidene-4-oxo-2-thioxothiazolidin-3-yl)alkanoic acids. Among them, 3-(4-phenylbutoxy)benzylidene derivatives 6f and 7f, endowed with interesting inhibitory activity against both targets, proved to control specific cellular pathways implicated in the development of T2DM and related complications.
K. Puls, and G. Wolber. Solving an Old Puzzle: Elucidation and Evaluation of the Binding Mode of Salvinorin A at the Kappa Opioid Receptor, Molecules, 28(2):718, 2023.
Links:
[doi:10.3390/molecules28020718]
[show BibTeX]
[show abstract]
x
@article{RN331,
author = {Puls, Kristina and Wolber, Gerhard},
title = {Solving an Old Puzzle: Elucidation and
Evaluation of the Binding Mode of
Salvinorin A at the Kappa Opioid
Receptor},
journal = {Molecules},
volume = {28},
number = {2},
pages = {718},
abstract = {The natural product Salvinorin A (SalA)
was the first nitrogen-lacking agonist
discovered for the opioid receptors and
exhibits high selectivity for the kappa
opioid receptor (KOR) turning SalA into a
promising analgesic to overcome the
current opioid crisis. Since SalA’s
suffers from poor pharmacokinetic
properties, particularly the absence of
gastrointestinal bioavailability, fast
metabolic inactivation, and subsequent
short duration of action, the rational
design of new tailored analogs with
improved clinical usability is highly
desired. Despite being known for decades,
the binding mode of SalA within the KOR
remains elusive as several conflicting
binding modes of SalA were proposed
hindering the rational design of new
analgesics. In this study, we rationally
determined the binding mode of SalA to the
active state KOR by in silico experiments
(docking, molecular dynamics simulations,
dynophores) in the context of all
available mutagenesis studies and
structure-activity relationship (SAR)
data. To the best of our knowledge, this
is the first comprehensive evaluation of
SalA’s binding mode since the
determination of the active state KOR
crystal structure. SalA binds above the
morphinan binding site with its furan
pointing toward the intracellular core
while the C2-acetoxy group is oriented
toward the extracellular loop 2 (ECL2).
SalA is solely stabilized within the
binding pocket by hydrogen bonds
(C210ECL2, Y3127.35, Y3137.36) and
hydrophobic contacts (V1182.63, I1393.33,
I2946.55, I3167.39). With the disruption
of this interaction pattern or the
establishment of additional interactions
within the binding site, we were able to
rationalize the experimental data for
selected analogs. We surmise the
C2-substituent interactions as important
for SalA and its analogs to be
experimentally active, albeit with
moderate frequency within MD simulations
of SalA. We further identified the
non-conserved residues 2.63, 7.35, and
7.36 responsible for the KOR subtype
selectivity of SalA. We are confident that
the elucidation of the SalA binding mode
will promote the understanding of KOR
activation and facilitate the development
of novel analgesics that are urgently
needed.},
ISSN = {1420-3049},
DOI = {10.3390/molecules28020718},
year = {2023},
type = {Journal Article}
}
x
Solving an Old Puzzle: Elucidation and Evaluation of the Binding Mode of Salvinorin A at the Kappa Opioid Receptor
The natural product Salvinorin A (SalA) was the first nitrogen-lacking agonist discovered for the opioid receptors and exhibits high selectivity for the kappa opioid receptor (KOR) turning SalA into a promising analgesic to overcome the current opioid crisis. Since SalA’s suffers from poor pharmacokinetic properties, particularly the absence of gastrointestinal bioavailability, fast metabolic inactivation, and subsequent short duration of action, the rational design of new tailored analogs with improved clinical usability is highly desired. Despite being known for decades, the binding mode of SalA within the KOR remains elusive as several conflicting binding modes of SalA were proposed hindering the rational design of new analgesics. In this study, we rationally determined the binding mode of SalA to the active state KOR by in silico experiments (docking, molecular dynamics simulations, dynophores) in the context of all available mutagenesis studies and structure-activity relationship (SAR) data. To the best of our knowledge, this is the first comprehensive evaluation of SalA’s binding mode since the determination of the active state KOR crystal structure. SalA binds above the morphinan binding site with its furan pointing toward the intracellular core while the C2-acetoxy group is oriented toward the extracellular loop 2 (ECL2). SalA is solely stabilized within the binding pocket by hydrogen bonds (C210ECL2, Y3127.35, Y3137.36) and hydrophobic contacts (V1182.63, I1393.33, I2946.55, I3167.39). With the disruption of this interaction pattern or the establishment of additional interactions within the binding site, we were able to rationalize the experimental data for selected analogs. We surmise the C2-substituent interactions as important for SalA and its analogs to be experimentally active, albeit with moderate frequency within MD simulations of SalA. We further identified the non-conserved residues 2.63, 7.35, and 7.36 responsible for the KOR subtype selectivity of SalA. We are confident that the elucidation of the SalA binding mode will promote the understanding of KOR activation and facilitate the development of novel analgesics that are urgently needed.
F. Ricci, K. Schira, L. Khettabi, L. Lombardo, S. Mirabile, R. Gitto, M. Soler-Lopez, J. Scheuermann, G. Wolber, and L. De Luca. Computational methods to analyze and predict the binding mode of inhibitors targeting both human and mushroom tyrosinase, Eur J Med Chem, 260:115771, 2023.
Links:
[doi:10.1016/j.ejmech.2023.115771]
[show BibTeX]
[show abstract]
x
@article{RN338,
author = {Ricci, Federico and Schira, Kristina and
Khettabi, Lyna and Lombardo, Lisa and
Mirabile, Salvatore and Gitto, Rosaria and
Soler-Lopez, Montserrat and Scheuermann,
Jörg and Wolber, Gerhard and De Luca,
Laura},
title = {Computational methods to analyze and
predict the binding mode of inhibitors
targeting both human and mushroom
tyrosinase},
journal = {European Journal of Medicinal Chemistry},
volume = {260},
pages = {115771},
abstract = {Tyrosinase, a copper-containing enzyme
critical in melanin biosynthesis, is a key
drug target for hyperpigmentation and
melanoma in humans. Testing the inhibitory
effects of compounds using tyrosinase from
Agaricus bisporus (AbTYR) has been a
common practice to identify potential
therapeutics from synthetic and natural
sources. However, structural diversity
among human tyrosinase (hTYR) and AbTYR
presents a challenge in developing drugs
that are therapeutically effective. In
this study, we combined retrospective and
computational analyses with experimental
data to provide insights into the
development of new inhibitors targeting
both hTYR and AbTYR. We observed
contrasting effects of Thiamidol™ and
our
4-(4-hydroxyphenyl)piperazin-1-yl-derivative
(6) on both enzymes; based on this
finding, we aimed to investigate their
binding modes in hTYR and AbTYR to
identify residues that significantly
improve affinity. All the information led
to the discovery of compound
[4-(4-hydroxyphenyl)piperazin-1-yl](2-methoxyphenyl)methanone
(MehT-3, 7), which showed comparable
activity on AbTYR (IC50 = 3.52 μM) and
hTYR (IC50 = 5.4 μM). Based on these
achievements we propose the exploitation
of our computational results to provide
relevant structural information for the
development of newer dual-targeting
molecules, which could be preliminarily
tested on AbTYR as a rapid and inexpensive
screening procedure before being tested on
hTYR.},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2023.115771},
url = {https://www.sciencedirect.com/science/article/pii/S0223523423007389
https://www.sciencedirect.com/science/article/pii/S0223523423007389?via%3Dihub},
year = {2023},
type = {Journal Article}
}
x
Computational methods to analyze and predict the binding mode of inhibitors targeting both human and mushroom tyrosinase
Tyrosinase, a copper-containing enzyme critical in melanin biosynthesis, is a key drug target for hyperpigmentation and melanoma in humans. Testing the inhibitory effects of compounds using tyrosinase from Agaricus bisporus (AbTYR) has been a common practice to identify potential therapeutics from synthetic and natural sources. However, structural diversity among human tyrosinase (hTYR) and AbTYR presents a challenge in developing drugs that are therapeutically effective. In this study, we combined retrospective and computational analyses with experimental data to provide insights into the development of new inhibitors targeting both hTYR and AbTYR. We observed contrasting effects of Thiamidol™ and our 4-(4-hydroxyphenyl)piperazin-1-yl-derivative (6) on both enzymes; based on this finding, we aimed to investigate their binding modes in hTYR and AbTYR to identify residues that significantly improve affinity. All the information led to the discovery of compound [4-(4-hydroxyphenyl)piperazin-1-yl](2-methoxyphenyl)methanone (MehT-3, 7), which showed comparable activity on AbTYR (IC50 = 3.52 μM) and hTYR (IC50 = 5.4 μM). Based on these achievements we propose the exploitation of our computational results to provide relevant structural information for the development of newer dual-targeting molecules, which could be preliminarily tested on AbTYR as a rapid and inexpensive screening procedure before being tested on hTYR.
Y. Shi, J. Li, C. A. Wolf, S. Liu, S. S. Sharma, G. Wolber, M. Bureik, and B. R. Clark. Expected and Unexpected Products from the Biochemical Oxidation of Bacterial Alkylquinolones with CYP4F11, J Nat Prod, :, 2023.
Links:
[doi:10.1021/acs.jnatprod.3c00689]
[show BibTeX]
[show abstract]
x
@article{RN344,
author = {Shi, Yue and Li, Jianye and Wolf, Clemens
Alexander and Liu, Sijie and Sharma,
Sangeeta S. and Wolber, Gerhard and
Bureik, Matthias and Clark, Benjamin R.},
title = {Expected and Unexpected Products from the
Biochemical Oxidation of Bacterial
Alkylquinolones with CYP4F11},
journal = {Journal of Natural Products},
note = {doi: 10.1021/acs.jnatprod.3c00689},
abstract = {2-Alkylquinolones are a class of
microbial natural products primarily
produced in the Pseudomonas and
Burkholderia genera that play a key role
in modulating quorum sensing. Bacterial
alkylquinolones were synthesized and then
subjected to oxidative biotransformation
using human cytochrome P450 enzyme
CYP4F11, heterologously expressed in the
fission yeast Schizosaccharomyces pombe.
This yielded a range of hydroxylated and
carboxylic acid derivatives which had
undergone ω-oxidation of the 2-alkyl
chain, the structures of which were
determined by analysis of NMR and MS data.
Oxidation efficiency depended on chain
length, with a chain length of eight or
nine carbon atoms proving optimal for high
yields. Homology modeling suggested that
Glu233 was relevant for binding, due to
the formation of a hydrogen bond from the
quinolone nitrogen to Glu233, and in this
position only the longer alkyl chains
could come close enough to the heme moiety
for effective oxidation. In addition to
the direct oxidation products, a number of
esters were also isolated, which was
attributed to the action of endogenous
yeast enzymes on the newly formed
ω-hydroxy-alkylquinolones. ω-Oxidation
of the alkyl chain significantly reduced
the antimicrobial and antibiofilm activity
of the quinolones.},
ISSN = {0163-3864},
DOI = {10.1021/acs.jnatprod.3c00689},
url = {https://doi.org/10.1021/acs.jnatprod.3c00689
https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.3c00689},
year = {2023},
type = {Journal Article}
}
x
Expected and Unexpected Products from the Biochemical Oxidation of Bacterial Alkylquinolones with CYP4F11
2-Alkylquinolones are a class of microbial natural products primarily produced in the Pseudomonas and Burkholderia genera that play a key role in modulating quorum sensing. Bacterial alkylquinolones were synthesized and then subjected to oxidative biotransformation using human cytochrome P450 enzyme CYP4F11, heterologously expressed in the fission yeast Schizosaccharomyces pombe. This yielded a range of hydroxylated and carboxylic acid derivatives which had undergone ω-oxidation of the 2-alkyl chain, the structures of which were determined by analysis of NMR and MS data. Oxidation efficiency depended on chain length, with a chain length of eight or nine carbon atoms proving optimal for high yields. Homology modeling suggested that Glu233 was relevant for binding, due to the formation of a hydrogen bond from the quinolone nitrogen to Glu233, and in this position only the longer alkyl chains could come close enough to the heme moiety for effective oxidation. In addition to the direct oxidation products, a number of esters were also isolated, which was attributed to the action of endogenous yeast enzymes on the newly formed ω-hydroxy-alkylquinolones. ω-Oxidation of the alkyl chain significantly reduced the antimicrobial and antibiofilm activity of the quinolones.
Y. Shi, C. A. Wolf, R. Lotfy, S. S. Sharma, A. F. Tesfa, G. Wolber, M. Bureik, and B. R. Clark. Deciphering the biotransformation mechanism of dialkylresorcinols by CYP4F11, Bioorg Chem, 131:106330, 2023.
Links:
[doi:10.1016/j.bioorg.2022.106330]
[show BibTeX]
[show abstract]
x
@article{RN330,
author = {Shi, Yue and Wolf, Clemens A. and Lotfy,
Rowaa and Sharma, Sangeeta S. and Tesfa,
Abel Fekadu and Wolber, Gerhard and
Bureik, Matthias and Clark, Benjamin R.},
title = {Deciphering the biotransformation
mechanism of dialkylresorcinols by
CYP4F11},
journal = {Bioorganic Chemistry},
volume = {131},
pages = {106330},
abstract = {Cytochrome P450 enzymes (CYPs) are one of
the most important classes of oxidative
enzymes in the human body, carrying out
metabolism of various exogenous and
endogenous substrates. In order to expand
the knowledge of these enzymes’
specificity and to obtain new natural
product derivatives, CYP4F11, a cytochrome
P450 monooxygenase, was used in the
biotransformation of dialkylresorcinols 1
and 2, a pair of antibiotic microbial
natural products. This investigation
resulted in four biotransformation
products including two oxidative products:
a hydroxylated derivative (3) and a
carboxylic acid derivative (4). In
addition, acetylated (5) and esterified
products (6) were isolated, formed by
further metabolism by endogenous yeast
enzymes. Oxidative transformations were
highly regioselective, and took place
exclusively at the ω-position of the C-5
alkyl chain. Homology modeling studies
revealed that optimal hydrogen bonding
between 2 and the enzyme can only be
established with the C-5 alkyl chain
pointing towards the heme. The
closely-related CYP4F12 was not capable of
oxidizing the dialkylresorcinol 2.
Modeling experiments rationalize these
differences by the different shapes of the
binding pockets with respect to the
non-oxidized alkyl chain. Antimicrobial
testing indicated that the presence of
polar groups on the side-chains reduces
the antibiotic activity of the
dialkylresorcinols.},
keywords = {Cytochrome P450 Homology modeling
Biotransformation Natural products
Dialkylresorcinols Fission yeast
Recombinant expression},
ISSN = {0045-2068},
DOI = {10.1016/j.bioorg.2022.106330},
url = {https://www.sciencedirect.com/science/article/pii/S0045206822007374},
year = {2023},
type = {Journal Article}
}
x
Deciphering the biotransformation mechanism of dialkylresorcinols by CYP4F11
Cytochrome P450 enzymes (CYPs) are one of the most important classes of oxidative enzymes in the human body, carrying out metabolism of various exogenous and endogenous substrates. In order to expand the knowledge of these enzymes’ specificity and to obtain new natural product derivatives, CYP4F11, a cytochrome P450 monooxygenase, was used in the biotransformation of dialkylresorcinols 1 and 2, a pair of antibiotic microbial natural products. This investigation resulted in four biotransformation products including two oxidative products: a hydroxylated derivative (3) and a carboxylic acid derivative (4). In addition, acetylated (5) and esterified products (6) were isolated, formed by further metabolism by endogenous yeast enzymes. Oxidative transformations were highly regioselective, and took place exclusively at the ω-position of the C-5 alkyl chain. Homology modeling studies revealed that optimal hydrogen bonding between 2 and the enzyme can only be established with the C-5 alkyl chain pointing towards the heme. The closely-related CYP4F12 was not capable of oxidizing the dialkylresorcinol 2. Modeling experiments rationalize these differences by the different shapes of the binding pockets with respect to the non-oxidized alkyl chain. Antimicrobial testing indicated that the presence of polar groups on the side-chains reduces the antibiotic activity of the dialkylresorcinols.
R. Wamser, S. Pach, C. Arkona, M. Baumgardt, U. B. A. Aziz, A. C. Hocke, G. Wolber, and J. Rademann. A Critical Study on Acylating and Covalent Reversible Fragment Inhibitors of SARS-CoV-2 Main Protease Targeting the S1 Site with Pyridine, ChemMedChem, 18:e202200635, 2023.
Links:
[doi:10.1002/cmdc.202200635]
[show BibTeX]
[show abstract]
x
@article{RN333,
author = {Wamser, Rebekka and Pach, Szymon and
Arkona, Christoph and Baumgardt, Morris
and Aziz, Umer Bin Abdul and Hocke,
Andreas C. and Wolber, Gerhard and
Rademann, Jörg},
title = {A Critical Study on Acylating and
Covalent Reversible Fragment Inhibitors of
SARS-CoV-2 Main Protease Targeting the S1
Site with Pyridine},
journal = {ChemMedChem},
volume = {18},
pages = {e202200635},
note = {https://doi.org/10.1002/cmdc.202200635},
abstract = {Abstract SARS coronavirus main proteases
(3CL proteases) have been validated as
pharmacological targets for the treatment
of coronavirus infections. Current
inhibitors of SARS main protease,
including the clinically admitted drug
nirmatrelvir are peptidomimetics with the
downsides of this class of drugs including
limited oral bioavailability, cellular
permeability, and rapid metabolic
degradation. Here, we investigate covalent
fragment inhibitors of SARS Mpro as
potential alternatives to peptidomimetic
inhibitors in use today. Starting from
inhibitors acylating the enzyme's active
site, a set of reactive fragments was
synthesized, and the inhibitory potency
was correlated with the chemical stability
of the inhibitors and the kinetic
stability of the covalent enzyme-inhibitor
complex. We found that all tested
acylating carboxylates, several of them
published prominently, were hydrolyzed in
assay buffer and the inhibitory
acyl-enzyme complexes were rapidly
degraded leading to the irreversible
inactivation of these drugs. Acylating
carbonates were found to be more stable
than acylating carboxylates, however, were
inactive in infected cells. Finally,
reversibly covalent fragments were
investigated as chemically stable SARS
CoV-2 inhibitors. Best was a
pyridine-aldehyde fragment with an IC50 of
1.8??M at a molecular weight of 211?g/mol,
showing that pyridine fragments indeed are
able to block the active site of
SARS-CoV-2 main protease.},
ISSN = {1860-7179},
DOI = {10.1002/cmdc.202200635},
url = {https://doi.org/10.1002/cmdc.202200635
https://chemistry-europe.onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cmdc.202200635?download= true},
year = {2023},
type = {Journal Article}
}
x
A Critical Study on Acylating and Covalent Reversible Fragment Inhibitors of SARS-CoV-2 Main Protease Targeting the S1 Site with Pyridine
Abstract SARS coronavirus main proteases (3CL proteases) have been validated as pharmacological targets for the treatment of coronavirus infections. Current inhibitors of SARS main protease, including the clinically admitted drug nirmatrelvir are peptidomimetics with the downsides of this class of drugs including limited oral bioavailability, cellular permeability, and rapid metabolic degradation. Here, we investigate covalent fragment inhibitors of SARS Mpro as potential alternatives to peptidomimetic inhibitors in use today. Starting from inhibitors acylating the enzyme's active site, a set of reactive fragments was synthesized, and the inhibitory potency was correlated with the chemical stability of the inhibitors and the kinetic stability of the covalent enzyme-inhibitor complex. We found that all tested acylating carboxylates, several of them published prominently, were hydrolyzed in assay buffer and the inhibitory acyl-enzyme complexes were rapidly degraded leading to the irreversible inactivation of these drugs. Acylating carbonates were found to be more stable than acylating carboxylates, however, were inactive in infected cells. Finally, reversibly covalent fragments were investigated as chemically stable SARS CoV-2 inhibitors. Best was a pyridine-aldehyde fragment with an IC50 of 1.8??M at a molecular weight of 211?g/mol, showing that pyridine fragments indeed are able to block the active site of SARS-CoV-2 main protease.
F. Wunsch, T. N. Nguyen, G. Wolber, and M. Bermudez. Structural determinants of sphingosine-1-phosphate receptor selectivity, Arch Pharm (Weinheim), :e2300387, 2023.
Links:
[doi:10.1002/ardp.202300387]
[show BibTeX]
[show abstract]
x
@article{RN340,
author = {Wunsch, F. and Nguyen, T. N. and Wolber,
G. and Bermudez, M.},
title = {Structural determinants of
sphingosine-1-phosphate receptor
selectivity},
journal = {Arch Pharm (Weinheim)},
pages = {e2300387},
note = {Wunsch, Friederike Nguyen, Trung Ngoc
Wolber, Gerhard Bermudez, Marcel eng
Joachim Herz Stiftung/ 407626949/German
Research Foundation (Deutsche
Forschungsgemeinschaft)/ Germany
2023/10/09 Arch Pharm (Weinheim). 2023 Oct
8:e2300387. doi: 10.1002/ardp.202300387.},
abstract = {Fingolimod, the prodrug of
fingolimod-1-phosphate (F1P), was the
first sphingosine-1-phosphate receptor
(S1PR) modulator approved for multiple
sclerosis. F1P unselectively targets all
five S1PR subtypes. While agonism
(functional antagonism via receptor
internalization) at S1PR(1) leads to the
desired immune modulatory effects, agonism
at S1PR(3) is associated with cardiac
adverse effects. This motivated the
development of S1PR(3) -sparing compounds
and led to a second generation of
S1PR(1,5) -selective ligands like
siponimod and ozanimod. Our method
combines molecular dynamics simulations
and three-dimensional pharmacophores
(dynophores) and enables the elucidation
of S1PR subtype-specific binding site
characteristics, visualizing also subtle
differences in receptor-ligand
interactions. F1P and the endogenous
ligand sphingosine-1-phosphate bind to the
orthosteric pocket of all S1PRs, but show
different binding mode dynamics,
uncovering potential starting points for
the development of subtype-specific
ligands. Our study contributes to the
mechanistic understanding of the
selectivity profile of approved drugs like
ozanimod and siponimod and pharmaceutical
tool compounds like CYM5541.},
keywords = {Gpcr drug design molecular dynamics
pharmacophores selectivity},
ISSN = {1521-4184 (Electronic) 0365-6233
(Linking)},
DOI = {10.1002/ardp.202300387},
url = {https://www.ncbi.nlm.nih.gov/pubmed/37806764
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/ardp.202300387?download= true},
year = {2023},
type = {Journal Article}
}
x
Structural determinants of sphingosine-1-phosphate receptor selectivity
Fingolimod, the prodrug of fingolimod-1-phosphate (F1P), was the first sphingosine-1-phosphate receptor (S1PR) modulator approved for multiple sclerosis. F1P unselectively targets all five S1PR subtypes. While agonism (functional antagonism via receptor internalization) at S1PR(1) leads to the desired immune modulatory effects, agonism at S1PR(3) is associated with cardiac adverse effects. This motivated the development of S1PR(3) -sparing compounds and led to a second generation of S1PR(1,5) -selective ligands like siponimod and ozanimod. Our method combines molecular dynamics simulations and three-dimensional pharmacophores (dynophores) and enables the elucidation of S1PR subtype-specific binding site characteristics, visualizing also subtle differences in receptor-ligand interactions. F1P and the endogenous ligand sphingosine-1-phosphate bind to the orthosteric pocket of all S1PRs, but show different binding mode dynamics, uncovering potential starting points for the development of subtype-specific ligands. Our study contributes to the mechanistic understanding of the selectivity profile of approved drugs like ozanimod and siponimod and pharmaceutical tool compounds like CYM5541.
J. Zhao, X. Zhang, Y. Wang, H. Huang, S. Sharma, S. S. Sharma, C. A. Wolf, S. Liu, G. Wolber, E. J. Sorensen, and M. Bureik. Exploring the chemical space of Proluciferins as probe substrates for human Cytochrome P450 enzymes, Appl Biochem Biotech, 195:1042-1058, 2023.
Links:
[doi:10.1007/s12010-022-04184-0]
[show BibTeX]
[show abstract]
x
@article{RN328,
author = {Zhao, Jie and Zhang, Xue and Wang, Yueyin
and Huang, Huimin and Sharma, Shishir and
Sharma, Sangeeta Shrestha and Wolf,
Clemens Alexander and Liu, Sijie and
Wolber, Gerhard and Sorensen, Erik J. and
Bureik, Matthias},
title = {Exploring the chemical space of
Proluciferins as probe substrates for
human Cytochrome P450 enzymes},
journal = {Applied Biochemistry and Biotechnology},
volume = {195},
pages = {1042-1058},
abstract = {We report the synthesis of 21 new
proluciferin compounds that bear a small
aliphatic ether group connected to the
6’ hydroxy function of firefly luciferin
and either contain an acid or methyl ester
function at the dihydrothiazole ring. Each
of these compounds was found to be a
substrate for some members of the human
CYP1 and CYP3 families; a total of 92 new
enzyme–substrate pairs were identified.
In a screen of the whole human P450
complement (CYPome) with three selected
proluciferin acid substrates, another 13
enzyme–substrate pairs were detected,
which involve enzymes belonging to the
CYP2, CYP4, CYP7, CYP21, and CYP27
families. All in all, we identified new
probe substrates for members of seven out
of 18 human CYP families.},
ISSN = {1559-0291},
DOI = {10.1007/s12010-022-04184-0},
url = {https://doi.org/10.1007/s12010-022-04184-0
https://link.springer.com/content/pdf/10.1007/s12010-022-04184-0.pdf},
year = {2023},
type = {Journal Article}
}
x
Exploring the chemical space of Proluciferins as probe substrates for human Cytochrome P450 enzymes
We report the synthesis of 21 new proluciferin compounds that bear a small aliphatic ether group connected to the 6’ hydroxy function of firefly luciferin and either contain an acid or methyl ester function at the dihydrothiazole ring. Each of these compounds was found to be a substrate for some members of the human CYP1 and CYP3 families; a total of 92 new enzyme–substrate pairs were identified. In a screen of the whole human P450 complement (CYPome) with three selected proluciferin acid substrates, another 13 enzyme–substrate pairs were detected, which involve enzymes belonging to the CYP2, CYP4, CYP7, CYP21, and CYP27 families. All in all, we identified new probe substrates for members of seven out of 18 human CYP families.
2022
[186]
D. Akman, K. Denzinger, S. Huang, J. T. Lee, J. W. Nafie, G. Wolber, G. W. Zamponi, D. W. Armstrong, and M. G. Gunduz. Focusing on C-4 position of Hantzsch 1,4-dihydropyridines: Molecular modifications, enantioseparation, and binding mechanism to L- and T-type calcium channels, Eur J Med Chem, 244:114787, 2022.
Links:
[doi:10.1016/j.ejmech.2022.114787]
[show BibTeX]
[show abstract]
x
@article{RN323,
author = {Akman, Dilara and Denzinger, Katrin and
Huang, Sun and Lee, J. T. and Nafie,
Jordan W. and Wolber, Gerhard and Zamponi,
Gerald W. and Armstrong, Daniel W. and
Gunduz, Miyase Gozde},
title = {Focusing on C-4 position of Hantzsch
1,4-dihydropyridines: Molecular
modifications, enantioseparation, and
binding mechanism to L- and T-type calcium
channels},
journal = {European Journal of Medicinal Chemistry},
volume = {244},
pages = {114787},
abstract = {1,4-Dihydropyridines (DHPs) represent the
blockbuster class of L-type calcium
channel blockers that have tremendous
therapeutic value against cardiovascular
conditions. Due to their abilities to
additionally target other subtypes of
calcium channels, DHPs are also considered
promising molecules for the treatment of
neurological and psychiatric disorders.
Having been in the market for more than
forty years, DHP is one of the most
modified scaffolds for the development of
novel molecules acting on calcium
channels. Taking the chemical structures
of approved DHPs into account, it is
noteworthy that C-4 position is the least
modified part of the ring system.
Therefore, in the present study, we
focused on this location and carried out
various molecular modifications to obtain
twelve potential calcium channel blockers
with a DHP-based hexahydroquinoline
scaffold (DA1-DA12). The whole-cell patch
clamp technique applied to analyze the
blocking ability of the synthesized
compounds on both L- (Cav1.2) and T-
(Cav3.2) type calcium channels revealed
five blockers with different selectivity
profiles. Introducing naphthyl moiety onto
the C-4 position of the main scaffold led
to the identification of a selective
blocker of Cav1.2 (DA8). The
benzodioxole-substituted derivative (DA1)
was the most potent and selective Cav3.2
inhibitor, therefore, its enantiomers were
separated using HPLC on a chiral
stationary phase. Retesting single isomers
on Cav3.2 revealed that S-enantiomer was
mainly responsible for the block. Finally,
DA compounds were docked into two
generated homology models of L- and T-type
calcium channels. Molecular dynamics (MD)
simulations and 3D pharmacophore modeling
provided further insights into the
detailed binding mechanism of DHPs to
Cav1.2 as well as to Cav3.2.},
keywords = {Hantzsch synthesis Hexahydroquinoline
Chiral center Patch clamp Molecular
dynamics 3D pharmacophore},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2022.114787},
url = {https://www.sciencedirect.com/science/article/pii/S0223523422006894},
year = {2022},
type = {Journal Article}
}
x
Focusing on C-4 position of Hantzsch 1,4-dihydropyridines: Molecular modifications, enantioseparation, and binding mechanism to L- and T-type calcium channels
1,4-Dihydropyridines (DHPs) represent the blockbuster class of L-type calcium channel blockers that have tremendous therapeutic value against cardiovascular conditions. Due to their abilities to additionally target other subtypes of calcium channels, DHPs are also considered promising molecules for the treatment of neurological and psychiatric disorders. Having been in the market for more than forty years, DHP is one of the most modified scaffolds for the development of novel molecules acting on calcium channels. Taking the chemical structures of approved DHPs into account, it is noteworthy that C-4 position is the least modified part of the ring system. Therefore, in the present study, we focused on this location and carried out various molecular modifications to obtain twelve potential calcium channel blockers with a DHP-based hexahydroquinoline scaffold (DA1-DA12). The whole-cell patch clamp technique applied to analyze the blocking ability of the synthesized compounds on both L- (Cav1.2) and T- (Cav3.2) type calcium channels revealed five blockers with different selectivity profiles. Introducing naphthyl moiety onto the C-4 position of the main scaffold led to the identification of a selective blocker of Cav1.2 (DA8). The benzodioxole-substituted derivative (DA1) was the most potent and selective Cav3.2 inhibitor, therefore, its enantiomers were separated using HPLC on a chiral stationary phase. Retesting single isomers on Cav3.2 revealed that S-enantiomer was mainly responsible for the block. Finally, DA compounds were docked into two generated homology models of L- and T-type calcium channels. Molecular dynamics (MD) simulations and 3D pharmacophore modeling provided further insights into the detailed binding mechanism of DHPs to Cav1.2 as well as to Cav3.2.
S. El Deeb, A. E. Ibrahim, A. Al-Harrasi, G. Wolber, and R. Gust. Validated capillary zone electrophoresis method for impurity profiling and determination of niII(3-ome-salophene), Separations, 9(2):25, 2022.
Links:
[doi:10.3390/separations9020025]
[show BibTeX]
[show abstract]
x
@article{RN317,
author = {El Deeb, Sami and Ibrahim, Adel Ehab and
Al-Harrasi, Ahmed and Wolber, Gerhard and
Gust, Ronald},
title = {Validated capillary zone electrophoresis
method for impurity profiling and
determination of niII(3-ome-salophene)},
journal = {Separations},
volume = {9},
number = {2},
pages = {25},
abstract = {A capillary zone electrophoresis method
was developed for the determination of
NiII(3-OMe-salophene), a substance with
anticancer activity in vitro. A fused
silica capillary (56 cm × 100 µm) was
used for this purpose. The method was
optimized in terms of parameters affecting
the electrophoretic conditions in order to
optimize separation efficiency and total
time of migration. The analysis was best
performed using an operating buffer of 50
mM borate, adjusted to pH 9.3, mixed with
acetonitrile (50%, v/v) as organic
modifier. Injections were performed
hydrodynamically by applying a pressure of
50 mbar for 8 s, and a 30 kV separation
voltage was selected at 25 ◦C. Detection
was carried out at 250 nm using diode
array detector (DAD). The method allowed
the separation of NiII(3-OMe-salophene)
from four other structurally related
impurities in a total migration time (tm)
of 8 min. Peak identification was achieved
using the standard reference of individual
impurities. The purity of the migrated
NiII(3-OMe-salophene) was confirmed by
Ultra-violet (UV) scan overlay depending
on DAD. The linear ranges for the
determination of NiII(3-OMe-salophene) was
400–20,000 ng mL−1 with limit of
detection (LOD) of 120 ng mL−1.
Acceptable intra-day and inter-day
precisions were achieved (%relative
standard deviation (RSD) results were less
than 0.76% and 0.30%, respectively). The
proposed method was assessed for greenness
and compared to reported methodologies to
prove superiority.},
ISSN = {2297-8739},
DOI = {10.3390/separations9020025},
year = {2022},
type = {Journal Article}
}
x
Validated capillary zone electrophoresis method for impurity profiling and determination of niII(3-ome-salophene)
A capillary zone electrophoresis method was developed for the determination of NiII(3-OMe-salophene), a substance with anticancer activity in vitro. A fused silica capillary (56 cm × 100 µm) was used for this purpose. The method was optimized in terms of parameters affecting the electrophoretic conditions in order to optimize separation efficiency and total time of migration. The analysis was best performed using an operating buffer of 50 mM borate, adjusted to pH 9.3, mixed with acetonitrile (50%, v/v) as organic modifier. Injections were performed hydrodynamically by applying a pressure of 50 mbar for 8 s, and a 30 kV separation voltage was selected at 25 ◦C. Detection was carried out at 250 nm using diode array detector (DAD). The method allowed the separation of NiII(3-OMe-salophene) from four other structurally related impurities in a total migration time (tm) of 8 min. Peak identification was achieved using the standard reference of individual impurities. The purity of the migrated NiII(3-OMe-salophene) was confirmed by Ultra-violet (UV) scan overlay depending on DAD. The linear ranges for the determination of NiII(3-OMe-salophene) was 400–20,000 ng mL−1 with limit of detection (LOD) of 120 ng mL−1. Acceptable intra-day and inter-day precisions were achieved (%relative standard deviation (RSD) results were less than 0.76% and 0.30%, respectively). The proposed method was assessed for greenness and compared to reported methodologies to prove superiority.
Y. Lu, X. Liu, R. Lotfy, S. Liu, A. F. Tesfa, G. Wolber, M. Bureik, and B. R. Clark. Experimental and computational studies on the biotransformation of pseudopyronines with human Cytochrome P450 CYP4F2, J Nat Prod, :, 2022.
Links:
[doi:10.1021/acs.jnatprod.2c00616]
[show BibTeX]
[show abstract]
x
@article{RN326,
author = {Lu, Ya and Liu, Xueling and Lotfy, Rowaa
and Liu, Sijie and Tesfa, Abel Fekadu and
Wolber, Gerhard and Bureik, Matthias and
Clark, Benjamin R.},
title = {Experimental and computational studies on
the biotransformation of pseudopyronines
with human Cytochrome P450 CYP4F2},
journal = {Journal of Natural Products},
note = {doi: 10.1021/acs.jnatprod.2c00616},
abstract = {The secondary metabolite pseudopyronine
B, isolated from Pseudomonas mosselii P33,
was biotransformed by human P450 enzymes,
heterologously expressed in the fission
yeast Schizosaccharomyces pombe.
Small-scale studies confirmed that both
CYP4F2 and CYP4F3A were capable of
oxidizing the substrate, with the former
achieving a higher yield. In larger-scale
studies using CYP4F2, three new oxidation
products were obtained, the structures of
which were elucidated by UV–vis, 1D and
2D NMR, and HR-MS spectroscopy. These
corresponded to hydroxylated,
carboxylated, and ester derivatives
(1–3) of pseudopyronine B, all of which
had been oxidized exclusively at the
ω-position of the C-6 alkyl chain. In
silico homology modeling experiments
highlighted key interactions between
oxygen atoms of the pyrone ring and two
serine residues and a histidine residue of
CYP4F2, which hold the substrate in a
suitable orientation for oxidation at the
terminus of the C-6 alkyl chain.
Additional modeling studies with all three
pseudopyronines revealed that the
seven-carbon alkyl chain of pseudopyronine
B was the perfect length for oxidation,
with the terminal carbon lying close to
the heme iron. The antibacterial activity
of the substrates and three oxidation
products was also assessed, revealing that
oxidation at the ω-position removes all
antimicrobial activity. This study both
increases the range of known substrates
for human CYF4F2 and CYP4F3A enzymes and
demonstrates their utility in producing
additional natural product derivatives.},
ISSN = {0163-3864},
DOI = {10.1021/acs.jnatprod.2c00616},
url = {https://doi.org/10.1021/acs.jnatprod.2c00616},
year = {2022},
type = {Journal Article}
}
x
Experimental and computational studies on the biotransformation of pseudopyronines with human Cytochrome P450 CYP4F2
The secondary metabolite pseudopyronine B, isolated from Pseudomonas mosselii P33, was biotransformed by human P450 enzymes, heterologously expressed in the fission yeast Schizosaccharomyces pombe. Small-scale studies confirmed that both CYP4F2 and CYP4F3A were capable of oxidizing the substrate, with the former achieving a higher yield. In larger-scale studies using CYP4F2, three new oxidation products were obtained, the structures of which were elucidated by UV–vis, 1D and 2D NMR, and HR-MS spectroscopy. These corresponded to hydroxylated, carboxylated, and ester derivatives (1–3) of pseudopyronine B, all of which had been oxidized exclusively at the ω-position of the C-6 alkyl chain. In silico homology modeling experiments highlighted key interactions between oxygen atoms of the pyrone ring and two serine residues and a histidine residue of CYP4F2, which hold the substrate in a suitable orientation for oxidation at the terminus of the C-6 alkyl chain. Additional modeling studies with all three pseudopyronines revealed that the seven-carbon alkyl chain of pseudopyronine B was the perfect length for oxidation, with the terminal carbon lying close to the heme iron. The antibacterial activity of the substrates and three oxidation products was also assessed, revealing that oxidation at the ω-position removes all antimicrobial activity. This study both increases the range of known substrates for human CYF4F2 and CYP4F3A enzymes and demonstrates their utility in producing additional natural product derivatives.
T. Noonan, K. Denzinger, V. Talagayev, Y. Chen, K. Puls, C. A. Wolf, S. Liu, T. N. Nguyen, and G. Wolber. Mind the gap - deciphering GPCR pharmacology using 3D pharmacophores and artificial intelligence, Pharmaceuticals, 15(11):1304, 2022.
Links:
[doi:10.3390/ph15111304]
[show BibTeX]
[show abstract]
x
@article{RN327,
author = {Noonan, Theresa and Denzinger, Katrin and
Talagayev, Valerij and Chen, Yu and Puls,
Kristina and Wolf, Clemens Alexander and
Liu, Sijie and Nguyen, Trung Ngoc and
Wolber, Gerhard},
title = {Mind the gap - deciphering GPCR
pharmacology using 3D pharmacophores and
artificial intelligence},
journal = {Pharmaceuticals},
volume = {15},
number = {11},
pages = {1304},
abstract = {G protein-coupled receptors (GPCRs) are
amongst the most pharmaceutically relevant
and well-studied protein targets, yet
unanswered questions in the field leave
significant gaps in our understanding of
their nuanced structure and function.
Three-dimensional pharmacophore models are
powerful computational tools in in silico
drug discovery, presenting myriad
opportunities for the integration of GPCR
structural biology and cheminformatics.
This review highlights success stories in
the application of 3D pharmacophore
modeling to de novo drug design, the
discovery of biased and allosteric
ligands, scaffold hopping, QSAR analysis,
hit-to-lead optimization, GPCR
de-orphanization, mechanistic
understanding of GPCR pharmacology and the
elucidation of ligand–receptor
interactions. Furthermore, advances in the
incorporation of dynamics and machine
learning are highlighted. The review will
analyze challenges in the field of GPCR
drug discovery, detailing how 3D
pharmacophore modeling can be used to
address them. Finally, we will present
opportunities afforded by 3D pharmacophore
modeling in the advancement of our
understanding and targeting of GPCRs},
ISSN = {1424-8247},
DOI = {10.3390/ph15111304},
url = {https://www.mdpi.com/1424-8247/15/11/1304},
year = {2022},
type = {Journal Article}
}
x
Mind the gap - deciphering GPCR pharmacology using 3D pharmacophores and artificial intelligence
G protein-coupled receptors (GPCRs) are amongst the most pharmaceutically relevant and well-studied protein targets, yet unanswered questions in the field leave significant gaps in our understanding of their nuanced structure and function. Three-dimensional pharmacophore models are powerful computational tools in in silico drug discovery, presenting myriad opportunities for the integration of GPCR structural biology and cheminformatics. This review highlights success stories in the application of 3D pharmacophore modeling to de novo drug design, the discovery of biased and allosteric ligands, scaffold hopping, QSAR analysis, hit-to-lead optimization, GPCR de-orphanization, mechanistic understanding of GPCR pharmacology and the elucidation of ligand–receptor interactions. Furthermore, advances in the incorporation of dynamics and machine learning are highlighted. The review will analyze challenges in the field of GPCR drug discovery, detailing how 3D pharmacophore modeling can be used to address them. Finally, we will present opportunities afforded by 3D pharmacophore modeling in the advancement of our understanding and targeting of GPCRs
K. Puls, A. Olivé-Marti, S. Pach, B. Pinter, F. Erli, G. Wolber, and M. Spetea. In vitro, in vivo and In silico characterization of a novel kappa-opioid receptor antagonist, Pharmaceuticals, 15(6):680, 2022.
Links:
[doi:10.3390/ph15060680]
[show BibTeX]
[show abstract]
x
@article{RN320,
author = {Puls, Kristina and Olivé-Marti,
Aina-Leonor and Pach, Szymon and Pinter,
Birgit and Erli, Filippo and Wolber,
Gerhard and Spetea, Mariana},
title = {In vitro, in vivo and In silico
characterization of a novel kappa-opioid
receptor antagonist},
journal = {Pharmaceuticals},
volume = {15},
number = {6},
pages = {680},
abstract = {Kappa-opioid receptor (KOR) antagonists
are promising innovative therapeutics for
the treatment of the central nervous
system (CNS) disorders. The new scaffold
opioid ligand, Compound A, was originally
found as a mu-opioid receptor (MOR)
antagonist but its binding/selectivity and
activation profile at the KOR and
delta-opioid receptor (DOR) remain
elusive. In this study, we present an in
vitro, in vivo and in silico
characterization of Compound A by
revealing this ligand as a KOR antagonist
in vitro and in vivo. In the radioligand
competitive binding assay, Compound A
bound at the human KOR, albeit with
moderate affinity, but with increased
affinity than to the human MOR and without
specific binding at the human DOR, thus
displaying a preferential KOR selectivity
profile. Following subcutaneous
administration in mice, Compound A
effectively reverse the antinociceptive
effects of the prototypical KOR agonist,
U50,488. In silico investigations were
carried out to assess the structural
determinants responsible for opioid
receptor subtype selectivity of Compound
A. Molecular docking, molecular dynamics
simulations and dynamic pharmacophore
(dynophore) generation revealed
differences in the stabilization of the
chlorophenyl moiety of Compound A within
the opioid receptor binding pockets,
rationalizing the experimentally
determined binding affinity values. This
new chemotype bears the potential for
favorable ADMET properties and holds
promise for chemical optimization toward
the development of potential therapeutics.
View Full-Text},
ISSN = {1424-8247},
DOI = {10.3390/ph15060680},
url = {https://www.mdpi.com/1424-8247/15/6/680},
year = {2022},
type = {Journal Article}
}
x
In vitro, in vivo and In silico characterization of a novel kappa-opioid receptor antagonist
Kappa-opioid receptor (KOR) antagonists are promising innovative therapeutics for the treatment of the central nervous system (CNS) disorders. The new scaffold opioid ligand, Compound A, was originally found as a mu-opioid receptor (MOR) antagonist but its binding/selectivity and activation profile at the KOR and delta-opioid receptor (DOR) remain elusive. In this study, we present an in vitro, in vivo and in silico characterization of Compound A by revealing this ligand as a KOR antagonist in vitro and in vivo. In the radioligand competitive binding assay, Compound A bound at the human KOR, albeit with moderate affinity, but with increased affinity than to the human MOR and without specific binding at the human DOR, thus displaying a preferential KOR selectivity profile. Following subcutaneous administration in mice, Compound A effectively reverse the antinociceptive effects of the prototypical KOR agonist, U50,488. In silico investigations were carried out to assess the structural determinants responsible for opioid receptor subtype selectivity of Compound A. Molecular docking, molecular dynamics simulations and dynamic pharmacophore (dynophore) generation revealed differences in the stabilization of the chlorophenyl moiety of Compound A within the opioid receptor binding pockets, rationalizing the experimentally determined binding affinity values. This new chemotype bears the potential for favorable ADMET properties and holds promise for chemical optimization toward the development of potential therapeutics. View Full-Text
K. Puls, H. Schmidhammer, G. Wolber, and M. Spetea. Mechanistic characterization of the pharmacological profile of hs-731, a peripherally acting opioid analgesic, at the µ-, δ, κ-opioid and nociceptin receptors, Molecules, 27(3):919, 2022.
Links:
[doi:10.3390/molecules27030919]
[show BibTeX]
[show abstract]
x
@article{RN318,
author = {Puls, Kristina and Schmidhammer, Helmut
and Wolber, Gerhard and Spetea, Mariana},
title = {Mechanistic characterization of the
pharmacological profile of hs-731, a
peripherally acting opioid analgesic, at
the µ-, δ, κ-opioid and nociceptin
receptors},
journal = {Molecules},
volume = {27},
number = {3},
pages = {919},
abstract = {Accumulated preclinical and clinical data
show that peripheral restricted opioids
provide pain relief with reduced side
effects. The peripherally acting opioid
analgesic HS-731 is a potent dual
μ-/δ-opioid receptor (MOR/DOR)
full agonist, and a weak, partial agonist
at the κ-opioid receptor (KOR).
However, its binding mode at the opioid
receptors remains elusive. Here, we
present a comprehensive in silico
evaluation of HS-731 binding at all opioid
receptors. We provide insights into
dynamic interaction patterns explaining
the different binding and activity of
HS-731 on the opioid receptors. For this
purpose, we conducted docking, performed
molecular dynamics (MD) simulations and
generated dynamic pharmacophores
(dynophores). Our results highlight two
residues important for HS-731 recognition
at the classical opioid receptors (MOR,
DOR and KOR), particular the conserved
residue 5.39 (K) and the non-conserved
residue 6.58 (MOR: K, DOR: W and KOR: E).
Furthermore, we assume a salt bridge
between the transmembrane helices (TM) 5
and 6 via K2275.39 and E2976.58 to be
responsible for the partial agonism of
HS-731 at the KOR. Additionally, we
experimentally demonstrated the absence of
affinity of HS-731 to the
nociceptin/orphanin FQ peptide (NOP)
receptor. We consider the morphinan phenol
Y1303.33 responsible for this affinity
lack. Y1303.33 points deep into the NOP
receptor binding pocket preventing HS-731
binding to the orthosteric binding pocket.
These findings provide significant
structural insights into HS-731
interaction pattern with the opioid
receptors that are important for
understanding the pharmacology of this
peripheral opioid analgesic.},
keywords = {GPCR opioid receptor HS-731 peripheral
opioid agonist analgesia binding
selectivity molecular docking molecular
dynamics simulations},
ISSN = {1420-3049},
DOI = {10.3390/molecules27030919},
year = {2022},
type = {Journal Article}
}
x
Mechanistic characterization of the pharmacological profile of hs-731, a peripherally acting opioid analgesic, at the µ-, δ, κ-opioid and nociceptin receptors
Accumulated preclinical and clinical data show that peripheral restricted opioids provide pain relief with reduced side effects. The peripherally acting opioid analgesic HS-731 is a potent dual μ-/δ-opioid receptor (MOR/DOR) full agonist, and a weak, partial agonist at the κ-opioid receptor (KOR). However, its binding mode at the opioid receptors remains elusive. Here, we present a comprehensive in silico evaluation of HS-731 binding at all opioid receptors. We provide insights into dynamic interaction patterns explaining the different binding and activity of HS-731 on the opioid receptors. For this purpose, we conducted docking, performed molecular dynamics (MD) simulations and generated dynamic pharmacophores (dynophores). Our results highlight two residues important for HS-731 recognition at the classical opioid receptors (MOR, DOR and KOR), particular the conserved residue 5.39 (K) and the non-conserved residue 6.58 (MOR: K, DOR: W and KOR: E). Furthermore, we assume a salt bridge between the transmembrane helices (TM) 5 and 6 via K2275.39 and E2976.58 to be responsible for the partial agonism of HS-731 at the KOR. Additionally, we experimentally demonstrated the absence of affinity of HS-731 to the nociceptin/orphanin FQ peptide (NOP) receptor. We consider the morphinan phenol Y1303.33 responsible for this affinity lack. Y1303.33 points deep into the NOP receptor binding pocket preventing HS-731 binding to the orthosteric binding pocket. These findings provide significant structural insights into HS-731 interaction pattern with the opioid receptors that are important for understanding the pharmacology of this peripheral opioid analgesic.
D. Stepanov, D. Buchmann, N. Schultze, G. Wolber, K. Schaufler, S. Guenther, and V. Belik. A combined bayesian and similarity-based approach for predicting E. coli biofilm inhibition by phenolic natural compounds, J Nat Prod, :, 2022.
Links:
[doi:10.1021/acs.jnatprod.2c00005]
[show BibTeX]
[show abstract]
x
@article{RN322,
author = {Stepanov, Dmitri and Buchmann, David and
Schultze, Nadin and Wolber, Gerhard and
Schaufler, Katharina and Guenther,
Sebastian and Belik, Vitaly},
title = {A combined bayesian and similarity-based
approach for predicting E. coli biofilm
inhibition by phenolic natural compounds},
journal = {Journal of Natural Products},
note = {doi: 10.1021/acs.jnatprod.2c00005},
abstract = {Screening for biofilm inhibition by
purified natural compounds is difficult
due to compounds’ chemical diversity and
limited commercial availability, combined
with time- and cost-intensiveness of the
laboratory process. In silico prediction
of chemical and biological properties of
molecules is a widely used technique when
experimental data availability is of
concern. At the same time, the performance
of predictive models directly depends on
the amount and quality of experimental
data. Driven by the interest in developing
a model for prediction of the antibiofilm
effect of phenolic natural compounds such
as flavonoids, we performed experimental
assessment of antibiofilm activity of 320
compounds from this subset of chemicals.
The assay was performed once on two
Escherichia coli strains on agar in
24-well microtiter plates. The inhibition
was assessed visually by detecting
morphological changes in macrocolonies.
Using the data obtained, we subsequently
trained a Bayesian logistic regression
model for prediction of biofilm
inhibition, which was combined with a
similarity-based method in order to
increase the overall sensitivity (at the
cost of accuracy). The quality of the
predictions was subsequently validated by
experimental assessment in three
independent experiments with two resistant
E. coli strains of 23 compounds absent in
the initial data set. The validation
demonstrated that the model may
successfully predict the targeted effect
as compared to the baseline accuracy.
Using a randomly selected database of
commercially available natural phenolics,
we obtained approximately 6.0% of active
compounds, whereas using our
prediction-based substance selection, the
percentage of phenolics found to be active
increased to 34.8%.},
ISSN = {0163-3864},
DOI = {10.1021/acs.jnatprod.2c00005},
url = {https://doi.org/10.1021/acs.jnatprod.2c00005},
year = {2022},
type = {Journal Article}
}
x
A combined bayesian and similarity-based approach for predicting E. coli biofilm inhibition by phenolic natural compounds
Screening for biofilm inhibition by purified natural compounds is difficult due to compounds’ chemical diversity and limited commercial availability, combined with time- and cost-intensiveness of the laboratory process. In silico prediction of chemical and biological properties of molecules is a widely used technique when experimental data availability is of concern. At the same time, the performance of predictive models directly depends on the amount and quality of experimental data. Driven by the interest in developing a model for prediction of the antibiofilm effect of phenolic natural compounds such as flavonoids, we performed experimental assessment of antibiofilm activity of 320 compounds from this subset of chemicals. The assay was performed once on two Escherichia coli strains on agar in 24-well microtiter plates. The inhibition was assessed visually by detecting morphological changes in macrocolonies. Using the data obtained, we subsequently trained a Bayesian logistic regression model for prediction of biofilm inhibition, which was combined with a similarity-based method in order to increase the overall sensitivity (at the cost of accuracy). The quality of the predictions was subsequently validated by experimental assessment in three independent experiments with two resistant E. coli strains of 23 compounds absent in the initial data set. The validation demonstrated that the model may successfully predict the targeted effect as compared to the baseline accuracy. Using a randomly selected database of commercially available natural phenolics, we obtained approximately 6.0% of active compounds, whereas using our prediction-based substance selection, the percentage of phenolics found to be active increased to 34.8%.
D. Thieme, P. Anielski, S. Rzeppa, C. A. Wolf, G. Wolber, and A. M. Keiler. Detection of 18-methyl steroids: Case report on a forensic urine sample and corresponding dietary supplements, Drug Testing and Analysis, 14(11-12):1864-1870, 2022.
Links:
[doi:10.1002/dta.3389]
[show BibTeX]
[show abstract]
x
@article{RN325,
author = {Thieme, Detlef and Anielski, Patricia and
Rzeppa, Sebastian and Wolf, Clemens A. and
Wolber, Gerhard and Keiler, Annekathrin
M.},
title = {Detection of 18-methyl steroids: Case
report on a forensic urine sample and
corresponding dietary supplements},
journal = {Drug Testing and Analysis},
volume = {14},
number = {11-12},
pages = {1864-1870},
abstract = {Abstract The detection of a putative
18-methyl-19-nortestosterone metabolite in
a forensic bodybuilder's urine sample
collected as part of a criminal proceeding
has triggered a follow-up investigation.
Four different dietary supplements in the
possession of the suspect were examined
with regard to possible precursor
steroids. This led to the detection of the
declared ingredient methoxydienone, which
was confirmed by both, GC–MSMS and
LC-HRMSMS. As neither
18-methyl-testosterone, nor
18-methyl-19-nortestosterone were
detectable in the supplements, the
possibility that the metabolite originates
from methoxydienone was investigated. For
this purpose, the metabolic fate of
methoxydienone was studied in vitro using
human HepG2 cells and in vivo by a single
oral administration. While the
18-methyl-19-nortestosterone metabolite
was not generated by HepG2 cells incubated
with methoxydienone, it was observed in
the urine samples collected at 2, 6, 10
and 24 h after methoxydienone
administration. Moreover, the potential
binding of methoxydienone as ligand to the
human androgen receptor was modelled in
silico in comparison with
18-methylnandrolone, for which androgen
receptor activation had been shown in an
in vitro approach before. In conclusion,
we could ascribe the presence of the
18-methyl-19-nortestosterone metabolite in
a forensic urine sample to originate from
methoxydienone present in dietary
supplements. Methoxydienone was observed
to slowly degrade by demethylation of the
methoxy substituent in liquid solutions.
While no compound-specific intermediates
were identified that allowed
differentiation from other 18-methyl
steroids, the 18-methyl-19-nortestosterone
metabolite proved to be a suitable marker
for reliable detection in doping
analysis.},
ISSN = {1942-7603},
DOI = {10.1002/dta.3389},
url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/dta.3389},
year = {2022},
type = {Journal Article}
}
x
Detection of 18-methyl steroids: Case report on a forensic urine sample and corresponding dietary supplements
Abstract The detection of a putative 18-methyl-19-nortestosterone metabolite in a forensic bodybuilder's urine sample collected as part of a criminal proceeding has triggered a follow-up investigation. Four different dietary supplements in the possession of the suspect were examined with regard to possible precursor steroids. This led to the detection of the declared ingredient methoxydienone, which was confirmed by both, GC–MSMS and LC-HRMSMS. As neither 18-methyl-testosterone, nor 18-methyl-19-nortestosterone were detectable in the supplements, the possibility that the metabolite originates from methoxydienone was investigated. For this purpose, the metabolic fate of methoxydienone was studied in vitro using human HepG2 cells and in vivo by a single oral administration. While the 18-methyl-19-nortestosterone metabolite was not generated by HepG2 cells incubated with methoxydienone, it was observed in the urine samples collected at 2, 6, 10 and 24 h after methoxydienone administration. Moreover, the potential binding of methoxydienone as ligand to the human androgen receptor was modelled in silico in comparison with 18-methylnandrolone, for which androgen receptor activation had been shown in an in vitro approach before. In conclusion, we could ascribe the presence of the 18-methyl-19-nortestosterone metabolite in a forensic urine sample to originate from methoxydienone present in dietary supplements. Methoxydienone was observed to slowly degrade by demethylation of the methoxy substituent in liquid solutions. While no compound-specific intermediates were identified that allowed differentiation from other 18-methyl steroids, the 18-methyl-19-nortestosterone metabolite proved to be a suitable marker for reliable detection in doping analysis.
F. Yang, S. Liu, G. Wolber, M. Bureik, and M. K. Parr. Complete reaction phenotyping of Propranolol and 4-Hydroxypropranolol with the 19 enzymes of the human UGT1 and UGT2 families, Int J Mol Sci, 23(13):7476, 2022.
Links:
[doi:10.3390/ijms23137476]
[show BibTeX]
[show abstract]
x
@article{RN321,
author = {Yang, Fan and Liu, Sijie and Wolber,
Gerhard and Bureik, Matthias and Parr,
Maria Kristina},
title = {Complete reaction phenotyping of
Propranolol and 4-Hydroxypropranolol with
the 19 enzymes of the human UGT1 and UGT2
families},
journal = {International Journal of Molecular
Sciences},
volume = {23},
number = {13},
pages = {7476},
abstract = {Propranolol is a competitive
non-selective beta-receptor antagonist
that is available on the market as a
racemic mixture. In the present study,
glucuronidation of propranolol and its
equipotent phase I metabolite
4-hydroxypropranolol by all 19 members of
the human UGT1 and UGT2 families was
monitored. UGT1A7, UGT1A9, UGT1A10 and
UGT2A1 were found to glucuronidate
propranolol, with UGT1A7, UGT1A9 and
UGT2A1 mainly acting on (S)-propranolol,
while UGT1A10 displays the opposite
stereoselectivity. UGT1A7, UGT1A9 and
UGT2A1 were also found to glucuronidate
4-hydroxypropranolol. In contrast to
propranolol, 4-hydroxypropranolol was
found to be glucuronidated by UGT1A8 but
not by UGT1A10. Additional
biotransformations with
4-methoxypropanolol demonstrated different
regioselectivities of these UGTs with
respect to the aliphatic and aromatic
hydroxy groups of the substrate. Modeling
and molecular docking studies were
performed to explain the stereoselective
glucuronidation of the substrates under
study.},
ISSN = {1422-0067},
DOI = {10.3390/ijms23137476},
url = {https://www.mdpi.com/1422-0067/23/13/7476},
year = {2022},
type = {Journal Article}
}
x
Complete reaction phenotyping of Propranolol and 4-Hydroxypropranolol with the 19 enzymes of the human UGT1 and UGT2 families
Propranolol is a competitive non-selective beta-receptor antagonist that is available on the market as a racemic mixture. In the present study, glucuronidation of propranolol and its equipotent phase I metabolite 4-hydroxypropranolol by all 19 members of the human UGT1 and UGT2 families was monitored. UGT1A7, UGT1A9, UGT1A10 and UGT2A1 were found to glucuronidate propranolol, with UGT1A7, UGT1A9 and UGT2A1 mainly acting on (S)-propranolol, while UGT1A10 displays the opposite stereoselectivity. UGT1A7, UGT1A9 and UGT2A1 were also found to glucuronidate 4-hydroxypropranolol. In contrast to propranolol, 4-hydroxypropranolol was found to be glucuronidated by UGT1A8 but not by UGT1A10. Additional biotransformations with 4-methoxypropanolol demonstrated different regioselectivities of these UGTs with respect to the aliphatic and aromatic hydroxy groups of the substrate. Modeling and molecular docking studies were performed to explain the stereoselective glucuronidation of the substrates under study.
J. Zhao, S. Liu, C. A. Wolf, G. Wolber, M. K. Parr, and M. Bureik. Changes in Alprazolam metabolism by CYP3A43 mutants, Biomedicines, 10(12):3022, 2022.
Links:
[doi:10.3390/biomedicines10123022]
[show BibTeX]
x
@article{RN329,
author = {Zhao, Jie and Liu, Sijie and Wolf,
Clemens Alexander and Wolber, Gerhard and
Parr, Maria Kristina and Bureik,
Matthias},
title = {Changes in Alprazolam metabolism by
CYP3A43 mutants},
journal = {Biomedicines},
volume = {10},
number = {12},
pages = {3022},
ISSN = {2227-9059},
DOI = {10.3390/biomedicines10123022},
url = {https://www.mdpi.com/2227-9059/10/12/3022},
year = {2022},
type = {Journal Article}
}
J. Zhao, D. Machalz, S. Liu, C. A. Wolf, G. Wolber, M. K. Parr, and M. Bureik. Metabolism of the antipsychotic drug olanzapine by CYP3A43, Xenobiotica, :1-29, 2022.
Links:
[doi:10.1080/00498254.2022.2078751]
[show BibTeX]
[show abstract]
x
@article{RN319,
author = {Zhao, Jie and Machalz, David and Liu,
Sijie and Wolf, Clemens Alexander and
Wolber, Gerhard and Parr, Maria Kristina
and Bureik, Matthias},
title = {Metabolism of the antipsychotic drug
olanzapine by CYP3A43},
journal = {Xenobiotica},
pages = {1-29},
note = {doi: 10.1080/00498254.2022.2078751},
abstract = {Abstract1. Olanzapine is an atypical
antipsychotic primarily used to treat
schizophrenia and bipolar disorder. An
intronic single nucleotide polymorphism
(SNP) that highly significantly predicts
increased olanzapine clearance (rs472660)
was previously identified in the CYP3A43
gene, which encodes a cytochrome P450
enzyme. But until now there was no
experimental evidence for the metabolism
of olanzapine by the CYP3A43 enzyme.2. In
the present study we provide this
evidence, together with a thorough
analysis of olanzapine metabolism by all
human CYP3A enzymes. We also rationalize
our findings by molecular docking
experiments. Moreover, we describe the
activities of several CYP3A43 mutants and
present the first enzymatic activity data
for the CYP3A43.3 variant; with respect to
prostate cancer, this polymorphic variant
is associated with both increased risk and
increased mortality. The catalytic
properties of the wild type enzyme and the
tumor mutant were analyzed by molecular
dynamics simulations, which fit very well
with the observed experimental results.3.
Our finding suggests that the SNP rs472660
likely causes an increased CYP3A43
expression level and demonstrate that,
depending on the substrate under study,
the tumor mutant CYP3A43.3 can have
increased activity in comparison to the
wild type enzyme CYP3A43.1.},
ISSN = {0049-8254},
DOI = {10.1080/00498254.2022.2078751},
url = {https://doi.org/10.1080/00498254.2022.2078751
https://www.tandfonline.com/doi/full/10.1080/00498254.2022.2078751},
year = {2022},
type = {Journal Article}
}
x
Metabolism of the antipsychotic drug olanzapine by CYP3A43
Abstract1. Olanzapine is an atypical antipsychotic primarily used to treat schizophrenia and bipolar disorder. An intronic single nucleotide polymorphism (SNP) that highly significantly predicts increased olanzapine clearance (rs472660) was previously identified in the CYP3A43 gene, which encodes a cytochrome P450 enzyme. But until now there was no experimental evidence for the metabolism of olanzapine by the CYP3A43 enzyme.2. In the present study we provide this evidence, together with a thorough analysis of olanzapine metabolism by all human CYP3A enzymes. We also rationalize our findings by molecular docking experiments. Moreover, we describe the activities of several CYP3A43 mutants and present the first enzymatic activity data for the CYP3A43.3 variant; with respect to prostate cancer, this polymorphic variant is associated with both increased risk and increased mortality. The catalytic properties of the wild type enzyme and the tumor mutant were analyzed by molecular dynamics simulations, which fit very well with the observed experimental results.3. Our finding suggests that the SNP rs472660 likely causes an increased CYP3A43 expression level and demonstrate that, depending on the substrate under study, the tumor mutant CYP3A43.3 can have increased activity in comparison to the wild type enzyme CYP3A43.1.
2021
[175]
A. Dolšak, D. Šribar, A. Scheffler, M. Grabowski, U. Švajger, S. Gobec, J. Holze, G. Weindl, G. Wolber, and M. Sova. Further hit optimization of 6-(trifluoromethyl)pyrimidin-2-amine based TLR8 modulators: Synthesis, biological evaluation and structure–activity relationships, Eur J Med Chem, 225:113809, 2021.
Links:
[doi:10.1016/j.ejmech.2021.113809]
[show BibTeX]
[show abstract]
x
@article{RN311,
author = {Dolšak, Ana and Šribar, Dora and
Scheffler, Alexander and Grabowski, Maria
and Švajger, Urban and Gobec, Stanislav
and Holze, Janine and Weindl, Günther and
Wolber, Gerhard and Sova, Matej},
title = {Further hit optimization of
6-(trifluoromethyl)pyrimidin-2-amine based
TLR8 modulators: Synthesis, biological
evaluation and structure–activity
relationships},
journal = {European Journal of Medicinal Chemistry},
volume = {225},
pages = {113809},
abstract = {Toll-like receptor 8 (TLR8) is an
endosomal TLR that has an important role
in the innate human immune system, which
is involved in numerous pathological
conditions. Excessive activation of TLR8
can lead to inflammatory and autoimmune
diseases, which highlights the need for
development of TLR8 modulators. However,
only a few small-molecule modulators that
selectively target TLR8 have been
developed. Here, we report the synthesis
and systematic investigation of the
structure–activity relationships of a
series of novel TLR8 negative modulators
based on previously reported
6-(trifluoromethyl)pyrimidin-2-amine
derivatives. Four compounds showed
low-micromolar concentration-dependent
inhibition of TLR8-mediated signaling in
HEK293 cells. These data confirm that the
6-trifluoromethyl group and two other
substituents on positions 2 and 4 are
important structural elements of
pyrimidine-based TLR8 modulators.
Substitution of the main scaffold at
position 2 with a methylsulfonyl group or
para hydroxy/hydroxymethyl substituted
benzylamine is essential for potent
negative modulation of TLR8. Our
best-in-class TLR8-selective modulator 53
with IC50 value of 6.2 μM represents a
promising small-molecule chemical probe
for further optimization to a lead
compound with potent immunomodulatory
properties.},
keywords = {Toll-like receptors TLR8 Modulators
Pyrimidines Immunomodulation Autoimmune
disorders},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2021.113809},
url = {https://www.sciencedirect.com/science/article/pii/S0223523421006589},
year = {2021},
type = {Journal Article}
}
x
Further hit optimization of 6-(trifluoromethyl)pyrimidin-2-amine based TLR8 modulators: Synthesis, biological evaluation and structure–activity relationships
Toll-like receptor 8 (TLR8) is an endosomal TLR that has an important role in the innate human immune system, which is involved in numerous pathological conditions. Excessive activation of TLR8 can lead to inflammatory and autoimmune diseases, which highlights the need for development of TLR8 modulators. However, only a few small-molecule modulators that selectively target TLR8 have been developed. Here, we report the synthesis and systematic investigation of the structure–activity relationships of a series of novel TLR8 negative modulators based on previously reported 6-(trifluoromethyl)pyrimidin-2-amine derivatives. Four compounds showed low-micromolar concentration-dependent inhibition of TLR8-mediated signaling in HEK293 cells. These data confirm that the 6-trifluoromethyl group and two other substituents on positions 2 and 4 are important structural elements of pyrimidine-based TLR8 modulators. Substitution of the main scaffold at position 2 with a methylsulfonyl group or para hydroxy/hydroxymethyl substituted benzylamine is essential for potent negative modulation of TLR8. Our best-in-class TLR8-selective modulator 53 with IC50 value of 6.2 μM represents a promising small-molecule chemical probe for further optimization to a lead compound with potent immunomodulatory properties.
M. Dumitrascuta, M. Bermudez, O. Trovato, J. De Neve, S. Ballet, G. Wolber, and M. Spetea. Antinociceptive efficacy of the μ-opioid/nociceptin peptide-based hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking, Molecules, 26(11):3267, 2021.
Links:
[doi:10.3390/molecules26113267]
[show BibTeX]
[show abstract]
x
@article{RN308,
author = {Dumitrascuta, Maria and Bermudez, Marcel
and Trovato, Olga and De Neve, Jolien and
Ballet, Steven and Wolber, Gerhard and
Spetea, Mariana},
title = {Antinociceptive efficacy of the
μ-opioid/nociceptin peptide-based hybrid
KGNOP1 in inflammatory pain without
rewarding effects in mice: an experimental
assessment and molecular docking},
journal = {Molecules},
volume = {26},
number = {11},
pages = {3267},
abstract = {Opioids are the most effective
analgesics, with most clinically available
opioids being agonists to the µ-opioid
receptor (MOR). The MOR is also
responsible for their unwanted effects,
including reward and opioid misuse leading
to the current public health crisis. The
imperative need for safer, non-addictive
pain therapies drives the search for novel
leads and new treatment strategies. In
this study, the recently discovered
MOR/nociceptin (NOP) receptor peptide
hybrid KGNOP1
(H-Dmt-D-Arg-Aba-β-Ala-Arg-Tyr-Tyr-Arg-Ile-Lys-NH2)
was evaluated following subcutaneous
administration in mouse models of acute
(formalin test) and chronic inflammatory
pain (Complete Freund’s adjuvant-induced
paw hyperalgesia), liabilities of
spontaneous locomotion, conditioned place
preference, and the withdrawal syndrome.
KGNOP1 demonstrated dose-dependent
antinociceptive effects in the formalin
test, and efficacy in attenuating thermal
hyperalgesia with prolonged duration of
action. Antinociceptive effects of KGNOP1
were reversed by naltrexone and SB-612111,
indicating the involvement of both MOR and
NOP receptor agonism. In comparison with
morphine, KGNOP1 was more potent and
effective in mouse models of inflammatory
pain. Unlike morphine, KGNOP1 displayed
reduced detrimental liabilities, as no
locomotor impairment nor rewarding and
withdrawal effects were observed. Docking
of KGNOP1 to the MOR and NOP receptors and
subsequent 3D interaction pattern analyses
provided valuable insights into its
binding mode. The mixed MOR/NOP receptor
peptide KGNOP1 holds promise in the effort
to develop new analgesics for the
treatment of various pain states with
fewer MOR-mediated side effects,
particularly abuse and dependence
liabilities.},
ISSN = {1420-3049},
DOI = {10.3390/molecules26113267},
url = {https://www.mdpi.com/1420-3049/26/11/3267
https://res.mdpi.com/d_attachment/molecules/molecules-26-03267/article_deploy/molecules-26-03267-v3.pdf},
year = {2021},
type = {Journal Article}
}
x
Antinociceptive efficacy of the μ-opioid/nociceptin peptide-based hybrid KGNOP1 in inflammatory pain without rewarding effects in mice: an experimental assessment and molecular docking
Opioids are the most effective analgesics, with most clinically available opioids being agonists to the µ-opioid receptor (MOR). The MOR is also responsible for their unwanted effects, including reward and opioid misuse leading to the current public health crisis. The imperative need for safer, non-addictive pain therapies drives the search for novel leads and new treatment strategies. In this study, the recently discovered MOR/nociceptin (NOP) receptor peptide hybrid KGNOP1 (H-Dmt-D-Arg-Aba-β-Ala-Arg-Tyr-Tyr-Arg-Ile-Lys-NH2) was evaluated following subcutaneous administration in mouse models of acute (formalin test) and chronic inflammatory pain (Complete Freund’s adjuvant-induced paw hyperalgesia), liabilities of spontaneous locomotion, conditioned place preference, and the withdrawal syndrome. KGNOP1 demonstrated dose-dependent antinociceptive effects in the formalin test, and efficacy in attenuating thermal hyperalgesia with prolonged duration of action. Antinociceptive effects of KGNOP1 were reversed by naltrexone and SB-612111, indicating the involvement of both MOR and NOP receptor agonism. In comparison with morphine, KGNOP1 was more potent and effective in mouse models of inflammatory pain. Unlike morphine, KGNOP1 displayed reduced detrimental liabilities, as no locomotor impairment nor rewarding and withdrawal effects were observed. Docking of KGNOP1 to the MOR and NOP receptors and subsequent 3D interaction pattern analyses provided valuable insights into its binding mode. The mixed MOR/NOP receptor peptide KGNOP1 holds promise in the effort to develop new analgesics for the treatment of various pain states with fewer MOR-mediated side effects, particularly abuse and dependence liabilities.
M. Janežič, K. Valjavec, K. B. Loboda, B. Herlah, I. Ogris, M. Kozorog, M. Podobnik, S. G. Grdadolnik, G. Wolber, and A. Perdih. Dynophore-based approach in virtual screening: a case of human DNA Topoisomerase IIα, Int J Mol Sci, 22(24):13474, 2021.
Links:
[doi:10.3390/ijms222413474]
[show BibTeX]
[show abstract]
x
@article{RN314,
author = {Janežič, Matej and Valjavec, Katja and
Loboda, Kaja Bergant and Herlah, Barbara
and Ogris, Iza and Kozorog, Mirijam and
Podobnik, Marjetka and Grdadolnik, Simona
Golič and Wolber, Gerhard and Perdih,
Andrej},
title = {Dynophore-based approach in virtual
screening: a case of human DNA
Topoisomerase IIα},
journal = {International Journal of Molecular
Sciences},
volume = {22},
number = {24},
pages = {13474},
abstract = {In this study, we utilized human DNA
topoisomerase IIα as a model target to
outline a dynophore-based approach to
catalytic inhibitor design. Based on MD
simulations of a known catalytic inhibitor
and the native ATP ligand analog, AMP-PNP,
we derived a joint dynophore model that
supplements the static
structure-based-pharmacophore information
with a dynamic component. Subsequently,
derived pharmacophore models were employed
in a virtual screening campaign of a
library of natural compounds. Experimental
evaluation identified flavonoid compounds
with promising topoisomerase IIα
catalytic inhibition and binding studies
confirmed interaction with the ATPase
domain. We constructed a binding model
through docking and extensively
investigated it with molecular dynamics MD
simulations, essential dynamics, and
MM-GBSA free energy calculations, thus
reconnecting the new results to the
initial dynophore-based screening model.
We not only demonstrate a new design
strategy that incorporates a dynamic
component of molecular recognition, but
also highlight new derivates in the
established flavonoid class of
topoisomerase II inhibitors.},
ISSN = {1422-0067},
DOI = {10.3390/ijms222413474},
url = {https://www.mdpi.com/1422-0067/22/24/13474},
year = {2021},
type = {Journal Article}
}
x
Dynophore-based approach in virtual screening: a case of human DNA Topoisomerase IIα
In this study, we utilized human DNA topoisomerase IIα as a model target to outline a dynophore-based approach to catalytic inhibitor design. Based on MD simulations of a known catalytic inhibitor and the native ATP ligand analog, AMP-PNP, we derived a joint dynophore model that supplements the static structure-based-pharmacophore information with a dynamic component. Subsequently, derived pharmacophore models were employed in a virtual screening campaign of a library of natural compounds. Experimental evaluation identified flavonoid compounds with promising topoisomerase IIα catalytic inhibition and binding studies confirmed interaction with the ATPase domain. We constructed a binding model through docking and extensively investigated it with molecular dynamics MD simulations, essential dynamics, and MM-GBSA free energy calculations, thus reconnecting the new results to the initial dynophore-based screening model. We not only demonstrate a new design strategy that incorporates a dynamic component of molecular recognition, but also highlight new derivates in the established flavonoid class of topoisomerase II inhibitors.
J. Y. Liu, D. Machalz, G. Wolber, E. J. Sorensen, and M. Bureik. New proluciferin substrates for human CYP4 family enzymes, Appl Biochem Biotech, 193(1):218-237, 2021.
Links:
[doi:10.1007/s12010-020-03388-6]
[show BibTeX]
[show abstract]
x
@article{RN282,
author = {Liu, J. Y. and Machalz, D. and Wolber, G.
and Sorensen, E. J. and Bureik, M.},
title = {New proluciferin substrates for human
CYP4 family enzymes},
journal = {Applied Biochemistry and Biotechnology},
volume = {193},
number = {1},
pages = {218-237},
note = {Nh3ox Times Cited:0 Cited References
Count:48},
abstract = {We report the synthesis of seven new
proluciferins for convenient activity
determination of enzymes belonging to the
cytochrome P450 (CYP) 4 family.
Biotransformation of these probe
substrates was monitored using each of the
twelve human CYP4 family members, and
eight were found to act at least on one of
them. For all substrates, activity of
CYP4Z1 was always highest, while that of
CYP4F8 was always second highest. Site of
metabolism (SOM) predictions involving
SMARTCyp and docking experiments helped to
rationalize the observed activity trends
linked to substrate accessibility and
reactivity. We further report the first
homology model of CYP4F8 including
suggested substrate recognition residues
in a catalytically competent conformation
accessed by replica exchange solute
tempering (REST) simulations.},
keywords = {cytochrome p450 docking fission yeast
homo sapiens pharmacology proluciferin
site of metabolism prediction human
cytochrome-p450 enzyme
functional-characterization
activation-energies prediction cancer
autoantibodies identification selectivity
expression smartcyp},
ISSN = {0273-2289},
DOI = {10.1007/s12010-020-03388-6},
url = {Go to ISI://WOS:000564584100001
https://link.springer.com/content/pdf/10.1007/s12010-020-03388-6.pdf},
year = {2021},
type = {Journal Article}
}
x
New proluciferin substrates for human CYP4 family enzymes
We report the synthesis of seven new proluciferins for convenient activity determination of enzymes belonging to the cytochrome P450 (CYP) 4 family. Biotransformation of these probe substrates was monitored using each of the twelve human CYP4 family members, and eight were found to act at least on one of them. For all substrates, activity of CYP4Z1 was always highest, while that of CYP4F8 was always second highest. Site of metabolism (SOM) predictions involving SMARTCyp and docking experiments helped to rationalize the observed activity trends linked to substrate accessibility and reactivity. We further report the first homology model of CYP4F8 including suggested substrate recognition residues in a catalytically competent conformation accessed by replica exchange solute tempering (REST) simulations.
S. Loke, A. Stoll, D. Machalz, F. Botrè, G. Wolber, M. Bureik, and M. K. Parr. Corticosteroid biosynthesis revisited: No direct hydroxylation of Pregnenolone by Steroid 21-Hydroxylase, Front Endocrinol, 12(629):, 2021.
Links:
[doi:10.3389/fendo.2021.633785]
[show BibTeX]
[show abstract]
x
@article{RN307,
author = {Loke, Steffen and Stoll, Anna and
Machalz, David and Botrè, Francesco and
Wolber, Gerhard and Bureik, Matthias and
Parr, Maria Kristina},
title = {Corticosteroid biosynthesis revisited: No
direct hydroxylation of Pregnenolone by
Steroid 21-Hydroxylase},
journal = {Frontiers in Endocrinology},
volume = {12},
number = {629},
abstract = {Cytochrome P450s (CYPs) are an essential
family of enzymes in the human body. They
play a crucial role in metabolism,
especially in human steroid biosynthesis.
Reactions catalyzed by these enzymes are
highly stereo- and regio-specific. Lack or
severe malfunctions of CYPs can cause
severe diseases and even shorten life.
Hence, investigations on metabolic
reactions and structural requirements of
substrates are crucial to gain further
knowledge on the relevance of different
enzymes in the human body functions and
the origin of diseases. One key enzyme in
the biosynthesis of gluco- and
mineralocorticoids is CYP21A2, also known
as steroid 21-hydroxylase. To investigate
the steric and regional requirements of
substrates for this enzyme, we performed
whole-cell biotransformation assays using
a strain of fission yeast
Schizosaccharomyces pombe recombinantly
expressing CYP21A2. The progestogens
progesterone, pregnenolone, and their
17α-hydroxy-derivatives were used as
substrates. After incubation, samples were
analyzed using gas chromatography coupled
to mass spectrometry. For progesterone and
17α-hydroxyprogesterone, their
corresponding 21-hydroxylated metabolites
11-deoxycorticosterone and
11-deoxycortisol were detected, while
after incubation of pregnenolone and
17α-hydroxypregnenolone, no hydroxylated
product was observed. Findings were
confirmed with authentic reference
material. Molecular docking experiments
agree with these results and suggest that
interaction between the 3-oxo group and
arginine-234 of the enzyme is a strict
requirement. The presented results
demonstrate once more that the presence of
an oxo-group in position 3 of the steroid
is indispensable, while a 3-hydroxy group
prevents hydroxylation in position C-21 by
CYP21A2. This knowledge may be transferred
to other CYP21A2 substrates and hence help
to gain essential insights into steroid
metabolism.},
keywords = {corticosteroid,cytochrome
P450,CYP21,GC-MS,Fission yeast
(Schizosaccharomyces pombe),molecular
docking,steroid biosynthesis},
ISSN = {1664-2392},
DOI = {10.3389/fendo.2021.633785},
url = {https://www.frontiersin.org/article/10.3389/fendo.2021.633785},
year = {2021},
type = {Journal Article}
}
x
Corticosteroid biosynthesis revisited: No direct hydroxylation of Pregnenolone by Steroid 21-Hydroxylase
Cytochrome P450s (CYPs) are an essential family of enzymes in the human body. They play a crucial role in metabolism, especially in human steroid biosynthesis. Reactions catalyzed by these enzymes are highly stereo- and regio-specific. Lack or severe malfunctions of CYPs can cause severe diseases and even shorten life. Hence, investigations on metabolic reactions and structural requirements of substrates are crucial to gain further knowledge on the relevance of different enzymes in the human body functions and the origin of diseases. One key enzyme in the biosynthesis of gluco- and mineralocorticoids is CYP21A2, also known as steroid 21-hydroxylase. To investigate the steric and regional requirements of substrates for this enzyme, we performed whole-cell biotransformation assays using a strain of fission yeast Schizosaccharomyces pombe recombinantly expressing CYP21A2. The progestogens progesterone, pregnenolone, and their 17α-hydroxy-derivatives were used as substrates. After incubation, samples were analyzed using gas chromatography coupled to mass spectrometry. For progesterone and 17α-hydroxyprogesterone, their corresponding 21-hydroxylated metabolites 11-deoxycorticosterone and 11-deoxycortisol were detected, while after incubation of pregnenolone and 17α-hydroxypregnenolone, no hydroxylated product was observed. Findings were confirmed with authentic reference material. Molecular docking experiments agree with these results and suggest that interaction between the 3-oxo group and arginine-234 of the enzyme is a strict requirement. The presented results demonstrate once more that the presence of an oxo-group in position 3 of the steroid is indispensable, while a 3-hydroxy group prevents hydroxylation in position C-21 by CYP21A2. This knowledge may be transferred to other CYP21A2 substrates and hence help to gain essential insights into steroid metabolism.
D. Machalz, H. Li, W. Du, S. Sharma, S. Liu, M. Bureik, and G. Wolber. Discovery of a novel potent cytochrome P450 CYP4Z1 inhibitor, Eur J Med Chem, 215:113255, 2021.
Links:
[doi:10.1016/j.ejmech.2021.113255]
[show BibTeX]
[show abstract]
x
@article{RN303,
author = {Machalz, David and Li, Hongjie and Du,
Wei and Sharma, Shishir and Liu, Sijie and
Bureik, Matthias and Wolber, Gerhard},
title = {Discovery of a novel potent cytochrome
P450 CYP4Z1 inhibitor},
journal = {European Journal of Medicinal Chemistry},
volume = {215},
pages = {113255},
abstract = {Human cytochrome P450 enzyme CYP4Z1
represents a promising target for the
treatment of a multitude of malignancies
including breast cancer. The most active
known non-covalent inhibitor
(1-benzylimidazole) only shows low
micromolar affinity to CYP4Z1. We report a
new, highly active inhibitor for CYP4Z1
showing confirmed binding in an enzymatic
assay and an IC50 value of 63 ± 19 nM
in stably transfected MCF-7 cells
overexpressing CYP4Z1. The new inhibitor
was identified by a systematically
developed virtual screening protocol.
Binding was rationalized using a carefully
elaborated 3D pharmacophore hypothesis and
thoroughly characterized using extensive
molecular dynamics simulations and dynamic
3D pharmacophore (dynophore) analyses.
This novel inhibitor represents a valuable
pharmacological tool to accelerate
characterization of the still understudied
CYP4Z1 and might pave the way for a new
treatment strategy in CYP4Z1-associated
malignancies. The presented in silico
model for predicting CYP4Z1 interaction
provides novel mechanistic insights and
revealed that the drug ozagrel interacts
with CYP4Z1.},
keywords = {Breast cancer CYP4Z1 Enzyme inhibition
Molecular modeling Virtual screening 3D
pharmacophores},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2021.113255},
url = {https://www.sciencedirect.com/science/article/pii/S0223523421001045},
year = {2021},
type = {Journal Article}
}
x
Discovery of a novel potent cytochrome P450 CYP4Z1 inhibitor
Human cytochrome P450 enzyme CYP4Z1 represents a promising target for the treatment of a multitude of malignancies including breast cancer. The most active known non-covalent inhibitor (1-benzylimidazole) only shows low micromolar affinity to CYP4Z1. We report a new, highly active inhibitor for CYP4Z1 showing confirmed binding in an enzymatic assay and an IC50 value of 63 ± 19 nM in stably transfected MCF-7 cells overexpressing CYP4Z1. The new inhibitor was identified by a systematically developed virtual screening protocol. Binding was rationalized using a carefully elaborated 3D pharmacophore hypothesis and thoroughly characterized using extensive molecular dynamics simulations and dynamic 3D pharmacophore (dynophore) analyses. This novel inhibitor represents a valuable pharmacological tool to accelerate characterization of the still understudied CYP4Z1 and might pave the way for a new treatment strategy in CYP4Z1-associated malignancies. The presented in silico model for predicting CYP4Z1 interaction provides novel mechanistic insights and revealed that the drug ozagrel interacts with CYP4Z1.
D. Machalz, S. Pach, M. Bermudez, M. Bureik, and G. Wolber. Structural insights into understudied human cytochrome P450 enzymes, Drug Discov Today, 26(10):2456-2464, 2021.
Links:
[doi:10.1016/j.drudis.2021.06.006]
[show BibTeX]
[show abstract]
x
@article{RN306,
author = {Machalz, David and Pach, Szymon and
Bermudez, Marcel and Bureik, Matthias and
Wolber, Gerhard},
title = {Structural insights into understudied
human cytochrome P450 enzymes},
journal = {Drug Discovery Today},
volume = {26},
number = {10},
pages = {2456-2464},
abstract = {Human cytochrome P450 (CYP) enzymes are
widely known for their pivotal role in the
metabolism of drugs and other xenobiotics
as well as of endogenous chemicals. In
addition, CYPs are involved in numerous
pathophysiological pathways and, hence,
are therapeutically relevant. Remarkably,
a portion of promising CYP targets is
still understudied and, as a consequence,
untargeted, despite their huge therapeutic
potential. An increasing number of X-ray
and cryo-electron microscopy (EM)
structures for CYPs have recently provided
new insights into the structural basis of
CYP function and potential ligand binding.
This structural knowledge of CYP
functionality is essential for both
understanding metabolism and exploiting
understudied CYPs as drug targets. In this
review, we summarize and highlight
structural knowledge about this enzyme
class, with a focus on understudied CYPs
and resulting opportunities for
structure-based drug design. Teaser: This
review summarizes recent structural
insights into understudied cytochrome P450
enzymes. We highlight the impact of
molecular modeling for mechanistically
explaining pathophysiological effects
establishing understudied CYPs as
promising drug targets.},
keywords = {CYP Cytochrome P450 Protein structure
Cancer Genetic disease Mutation Homology
modeling Metabolism Molecular modeling},
ISSN = {1359-6446},
DOI = {10.1016/j.drudis.2021.06.006},
url = {https://www.sciencedirect.com/science/article/pii/S1359644621002786
https://www.sciencedirect.com/science/article/abs/pii/S1359644621002786?via%3Dihub},
year = {2021},
type = {Journal Article}
}
x
Structural insights into understudied human cytochrome P450 enzymes
Human cytochrome P450 (CYP) enzymes are widely known for their pivotal role in the metabolism of drugs and other xenobiotics as well as of endogenous chemicals. In addition, CYPs are involved in numerous pathophysiological pathways and, hence, are therapeutically relevant. Remarkably, a portion of promising CYP targets is still understudied and, as a consequence, untargeted, despite their huge therapeutic potential. An increasing number of X-ray and cryo-electron microscopy (EM) structures for CYPs have recently provided new insights into the structural basis of CYP function and potential ligand binding. This structural knowledge of CYP functionality is essential for both understanding metabolism and exploiting understudied CYPs as drug targets. In this review, we summarize and highlight structural knowledge about this enzyme class, with a focus on understudied CYPs and resulting opportunities for structure-based drug design. Teaser: This review summarizes recent structural insights into understudied cytochrome P450 enzymes. We highlight the impact of molecular modeling for mechanistically explaining pathophysiological effects establishing understudied CYPs as promising drug targets.
T. Meşeli, S. D. Doğan, M. G. Gündüz, Z. Kökbudak, S. Skaro Bogojevic, T. Noonan, S. Vojnovic, G. Wolber, and J. Nikodinovic-Runic. Design, synthesis, antibacterial activity evaluation and molecular modeling studies of new sulfonamides containing a sulfathiazole moiety, New J Chem, 45(18):8166-8177, 2021.
Links:
[doi:10.1039/D1NJ00150G]
[show BibTeX]
[show abstract]
x
@article{RN309,
author = {Meşeli, Tuğba and Doğan, Sengül Dilem
and Gündüz, Miyase Gözde and Kökbudak,
Zülbiye and Skaro Bogojevic, Sanja and
Noonan, Theresa and Vojnovic, Sandra and
Wolber, Gerhard and Nikodinovic-Runic,
Jasmina},
title = {Design, synthesis, antibacterial activity
evaluation and molecular modeling studies
of new sulfonamides containing a
sulfathiazole moiety},
journal = {New Journal of Chemistry},
volume = {45},
number = {18},
pages = {8166-8177},
abstract = {Sulfonamides represent the oldest
synthetic antibacterial agents; however,
their central position in controlling
bacterial diseases has been seriously
damaged by the development of widespread
resistance. Herein, we revisited
sulfathiazole, a commercial member of
antibacterial sulfa drugs, intending to
overcome sulfonamide resistance and
identify new drug candidates through
molecular modifications. We synthesized
twelve sulfonamides (SA1–SA12) by
replacing the amino group on the phenyl
ring with various substituents and
introducing a thiophene ring on the core
scaffold of sulfathiazole. The obtained
compounds and additionally two commercial
sulfonamides, sulfathiazole and
sulfadiazine, were extensively screened
for their antimicrobial activities. The
results indicated that new sulfonamides,
unlike traditional ones, were selectively
effective against various Staphylococcus
aureus strains. Introducing a bulky
lipophilic substituent at the para
position of the phenyl ring significantly
increased the antibacterial activities of
the compounds against Staphylococcus
aureus. The compounds demonstrating
favourable selectivity indices were
further evaluated for their membrane
potential perturbation and DNA interaction
properties. The obtained data showed that
these are not supporting mechanisms for
the antibacterial activities of the
modified sulfathiazole derivatives. In
order to rationalize the activity of the
three most active compounds, SA7, SA11 and
SA12, against S. aureus ATCC 25923, their
binding hypotheses within the catalytic
site of Staphylococcus aureus
dihydropteroate synthase, the validated
target enzyme of sulfonamides, were
generated via molecular docking and
further dissected using molecular dynamics
simulations and dynamic 3D pharmacophores
(dynophores).},
ISSN = {1144-0546},
DOI = {10.1039/D1NJ00150G},
url = {http://dx.doi.org/10.1039/D1NJ00150G
https://pubs.rsc.org/en/content/articlepdf/2021/nj/d1nj00150g},
year = {2021},
type = {Journal Article}
}
x
Design, synthesis, antibacterial activity evaluation and molecular modeling studies of new sulfonamides containing a sulfathiazole moiety
Sulfonamides represent the oldest synthetic antibacterial agents; however, their central position in controlling bacterial diseases has been seriously damaged by the development of widespread resistance. Herein, we revisited sulfathiazole, a commercial member of antibacterial sulfa drugs, intending to overcome sulfonamide resistance and identify new drug candidates through molecular modifications. We synthesized twelve sulfonamides (SA1–SA12) by replacing the amino group on the phenyl ring with various substituents and introducing a thiophene ring on the core scaffold of sulfathiazole. The obtained compounds and additionally two commercial sulfonamides, sulfathiazole and sulfadiazine, were extensively screened for their antimicrobial activities. The results indicated that new sulfonamides, unlike traditional ones, were selectively effective against various Staphylococcus aureus strains. Introducing a bulky lipophilic substituent at the para position of the phenyl ring significantly increased the antibacterial activities of the compounds against Staphylococcus aureus. The compounds demonstrating favourable selectivity indices were further evaluated for their membrane potential perturbation and DNA interaction properties. The obtained data showed that these are not supporting mechanisms for the antibacterial activities of the modified sulfathiazole derivatives. In order to rationalize the activity of the three most active compounds, SA7, SA11 and SA12, against S. aureus ATCC 25923, their binding hypotheses within the catalytic site of Staphylococcus aureus dihydropteroate synthase, the validated target enzyme of sulfonamides, were generated via molecular docking and further dissected using molecular dynamics simulations and dynamic 3D pharmacophores (dynophores).
R. Ottanà, P. Paoli, M. Cappiello, T. N. Nguyen, I. Adornato, A. Del Corso, M. Genovese, I. Nesi, R. Moschini, A. Naß, G. Wolber, and R. Maccari. In search for multi-target ligands as potential agents for diabetes mellitus and its complications—a structure-activity relationship study on inhibitors of aldose reductase and Protein Tyrosine Phosphatase 1B, Molecules, 26(2):330, 2021.
Links:
[doi:10.3390/molecules26020330]
[show BibTeX]
[show abstract]
x
@article{RN299,
author = {Ottanà, Rosaria and Paoli, Paolo and
Cappiello, Mario and Nguyen, Trung Ngoc
and Adornato, Ilenia and Del Corso,
Antonella and Genovese, Massimo and Nesi,
Ilaria and Moschini, Roberta and Naß,
Alexandra and Wolber, Gerhard and Maccari,
Rosanna},
title = {In search for multi-target ligands as
potential agents for diabetes mellitus and
its complications—a structure-activity
relationship study on inhibitors of aldose
reductase and Protein Tyrosine Phosphatase
1B},
journal = {Molecules},
volume = {26},
number = {2},
pages = {330},
abstract = {Diabetes mellitus (DM) is a complex
disease which currently affects more than
460 million people and is one of the
leading cause of death worldwide. Its
development implies numerous metabolic
dysfunctions and the onset of
hyperglycaemia-induced chronic
complications. Multiple ligands can be
rationally designed for the treatment of
multifactorial diseases, such as DM, with
the precise aim of simultaneously
controlling multiple pathogenic mechanisms
related to the disease and providing a
more effective and safer therapeutic
treatment compared to combinations of
selective drugs. Starting from our
previous findings that highlighted the
possibility to target both aldose
reductase (AR) and protein tyrosine
phosphatase 1B (PTP1B), two enzymes
strictly implicated in the development of
DM and its complications, we synthesised
3-(5-arylidene-4-oxothiazolidin-3-yl)propanoic
acids and analogous 2-butenoic acid
derivatives, with the aim of balancing the
effectiveness of dual AR/PTP1B inhibitors
which we had identified as designed
multiple ligands (DMLs). Out of the tested
compounds, 4f exhibited well-balanced
AR/PTP1B inhibitory effects at low
micromolar concentrations, along with
interesting insulin-sensitizing activity
in murine C2C12 cell cultures. The SARs
here highlighted along with their
rationalization by in silico docking
experiments into both target enzymes
provide further insights into this class
of inhibitors for their development as
potential DML antidiabetic candidates.},
ISSN = {1420-3049},
DOI = {10.3390/molecules26020330},
url = {https://www.mdpi.com/1420-3049/26/2/330
https://res.mdpi.com/d_attachment/molecules/molecules-26-00330/article_deploy/molecules-26-00330-v3.pdf},
year = {2021},
type = {Journal Article}
}
x
In search for multi-target ligands as potential agents for diabetes mellitus and its complications—a structure-activity relationship study on inhibitors of aldose reductase and Protein Tyrosine Phosphatase 1B
Diabetes mellitus (DM) is a complex disease which currently affects more than 460 million people and is one of the leading cause of death worldwide. Its development implies numerous metabolic dysfunctions and the onset of hyperglycaemia-induced chronic complications. Multiple ligands can be rationally designed for the treatment of multifactorial diseases, such as DM, with the precise aim of simultaneously controlling multiple pathogenic mechanisms related to the disease and providing a more effective and safer therapeutic treatment compared to combinations of selective drugs. Starting from our previous findings that highlighted the possibility to target both aldose reductase (AR) and protein tyrosine phosphatase 1B (PTP1B), two enzymes strictly implicated in the development of DM and its complications, we synthesised 3-(5-arylidene-4-oxothiazolidin-3-yl)propanoic acids and analogous 2-butenoic acid derivatives, with the aim of balancing the effectiveness of dual AR/PTP1B inhibitors which we had identified as designed multiple ligands (DMLs). Out of the tested compounds, 4f exhibited well-balanced AR/PTP1B inhibitory effects at low micromolar concentrations, along with interesting insulin-sensitizing activity in murine C2C12 cell cultures. The SARs here highlighted along with their rationalization by in silico docking experiments into both target enzymes provide further insights into this class of inhibitors for their development as potential DML antidiabetic candidates.
S. Pach, T. N. Nguyen, J. Trimpert, D. Kunec, N. Osterrieder, and G. Wolber. ACE2 variants indicate potential SARS-CoV-2-Susceptibility in animals: An extensive molecular dynamics study, Mol Inf, 40(9):2100031, 2021.
Links:
[doi:10.1002/minf.202100031]
[show BibTeX]
x
@article{RN310,
author = {Pach, Szymon and Nguyen, Trung Ngoc and
Trimpert, Jakob and Kunec, Dusan and
Osterrieder, Nikolaus and Wolber,
Gerhard},
title = {ACE2 variants indicate potential
SARS-CoV-2-Susceptibility in animals: An
extensive molecular dynamics study},
journal = {Molecular Informatics},
volume = {40},
number = {9},
pages = {2100031},
DOI = {10.1002/minf.202100031},
url = {https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/minf.202100031?download=true},
year = {2021},
type = {Journal Article}
}
S. Sharma, S. Liu, P. Durairaj, D. Machalz, G. Wolber, and M. Bureik. A convenient test system for the identification of CYP4V2 inhibitors, Mol Vis, 27:601-607, 2021.
Links:
[show BibTeX]
[show abstract]
x
@article{RN312,
author = {Sharma, Shishir and Liu, Sijie and
Durairaj, Pradeepraj and Machalz, David
and Wolber, Gerhard and Bureik, Matthias},
title = {A convenient test system for the
identification of CYP4V2 inhibitors},
journal = {Molecular Vision},
volume = {27},
pages = {601-607},
note = {mv-v27-601.pdf},
abstract = {Purpose: Polymorphisms in the gene that
codes for the human cytochrome P450 enzyme
CYP4V2 are a cause of Bietti crystalline
dystrophy (BCD). Therefore, inhibition of
CYP4V2 activity may well be a cause of
visual disability. However, monitoring the
fatty acid hydroxylation reactions
catalyzed by this enzyme is tedious and
not well suited for inhibitor screening.
Methods: We investigated the use of
proluciferin compounds as probe substrates
for efficient and convenient determination
of CYP4V2 activity. Results: Ten
proluciferins were tested for conversion
by CYP4V2, and eight were found to be
substrates of this enzyme. One point
inhibitor assays were performed using
luciferin 6' 3-furfuryl ether methyl ester
(luciferin-3FEME) as the probe substrate
and 12 test compounds. As expected,
HET0016 had by far the strongest effect,
while two other compounds (including
osilodrostat) also displayed statistically
significant inhibitory potency. The half
maximal inhibitory concentration (IC50)
for HET0016 was determined to be 179 nM. A
recently identified potent inhibitor of
human CYP4Z1 was found not to inhibit
CYP4V2. To explore the selectivity of this
compound between CYP4Z1 and CYP4V2, we
developed a homology model of CYP4V2 and
conducted docking experiments.
Conclusions: We provide the first protocol
for a robust and convenient CYP4V2
inhibitor assay that does not depend on
fatty acid analysis but can be simply
monitored with luminescence. Moreover, we
demonstrate additional evidence for the
concern that compounds with CYP-inhibitory
properties may inhibit CYP4V2 activity and
thus, possibly cause visual disability.},
url = {http://www.molvis.org/molvis/v27/601},
year = {2021},
type = {Journal Article}
}
x
A convenient test system for the identification of CYP4V2 inhibitors
Purpose: Polymorphisms in the gene that codes for the human cytochrome P450 enzyme CYP4V2 are a cause of Bietti crystalline dystrophy (BCD). Therefore, inhibition of CYP4V2 activity may well be a cause of visual disability. However, monitoring the fatty acid hydroxylation reactions catalyzed by this enzyme is tedious and not well suited for inhibitor screening. Methods: We investigated the use of proluciferin compounds as probe substrates for efficient and convenient determination of CYP4V2 activity. Results: Ten proluciferins were tested for conversion by CYP4V2, and eight were found to be substrates of this enzyme. One point inhibitor assays were performed using luciferin 6' 3-furfuryl ether methyl ester (luciferin-3FEME) as the probe substrate and 12 test compounds. As expected, HET0016 had by far the strongest effect, while two other compounds (including osilodrostat) also displayed statistically significant inhibitory potency. The half maximal inhibitory concentration (IC50) for HET0016 was determined to be 179 nM. A recently identified potent inhibitor of human CYP4Z1 was found not to inhibit CYP4V2. To explore the selectivity of this compound between CYP4Z1 and CYP4V2, we developed a homology model of CYP4V2 and conducted docking experiments. Conclusions: We provide the first protocol for a robust and convenient CYP4V2 inhibitor assay that does not depend on fatty acid analysis but can be simply monitored with luminescence. Moreover, we demonstrate additional evidence for the concern that compounds with CYP-inhibitory properties may inhibit CYP4V2 activity and thus, possibly cause visual disability.
C. Tauber, R. Wamser, C. Arkona, M. Tuegend, U. B. Abdul Aziz, S. Pach, R. Schulz, D. Jochmans, G. Wolber, J. Neyts, and J. Rademann. Chemical evolution of antivirals against enterovirus D68 through protein-templated Knoevenagel reactions, Angewandte Chemie International Edition, 60(24):13294-13301, 2021.
Links:
[doi:10.1002/anie.202102074]
[show BibTeX]
[show abstract]
x
@article{RN305,
author = {Tauber, Carolin and Wamser, Rebekka and
Arkona, Christoph and Tuegend, Marisa and
Abdul Aziz, Umer Bin and Pach, Szymon and
Schulz, Robert and Jochmans, Dirk and
Wolber, Gerhard and Neyts, Johan and
Rademann, Joerg},
title = {Chemical evolution of antivirals against
enterovirus D68 through protein-templated
Knoevenagel reactions},
journal = {Angewandte Chemie International Edition},
volume = {60},
number = {24},
pages = {13294-13301},
note = {https://doi.org/10.1002/anie.202102074},
abstract = {The generation of bioactive molecules
from inactive precursors is a crucial step
in the chemical evolution of life,
however, mechanistic insights into this
aspect of abiogenesis are scarce. Here, we
investigate the protein-catalyzed
formation of antivirals by the 3C-protease
of enterovirus D68. The enzyme induces
aldol condensations yielding inhibitors
with antiviral activity in cells. Kinetic
and thermodynamic analyses reveal that the
bioactivity emerges from a dynamic
reaction system including inhibitor
formation, alkylation of the protein
target by the inhibitors, and competitive
addition of non-protein nucleophiles to
the inhibitors. The most active antivirals
are slowly reversible inhibitors with
elongated target residence times. The
study reveals first examples for the
chemical evolution of bio-actives through
protein-catalyzed, non-enzymatic
CC-couplings. The discovered mechanism
works under physiological conditions and
might constitute a native process of drug
development.},
keywords = {Chemical Evolution, Protein-templated
reactions, Antivirals, Protease
inhibitors, Fragment-based drug
discovery},
ISSN = {1433-7851},
DOI = {10.1002/anie.202102074},
url = {https://doi.org/10.1002/anie.202102074
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/anie.202102074?download= true},
year = {2021},
type = {Journal Article}
}
x
Chemical evolution of antivirals against enterovirus D68 through protein-templated Knoevenagel reactions
The generation of bioactive molecules from inactive precursors is a crucial step in the chemical evolution of life, however, mechanistic insights into this aspect of abiogenesis are scarce. Here, we investigate the protein-catalyzed formation of antivirals by the 3C-protease of enterovirus D68. The enzyme induces aldol condensations yielding inhibitors with antiviral activity in cells. Kinetic and thermodynamic analyses reveal that the bioactivity emerges from a dynamic reaction system including inhibitor formation, alkylation of the protein target by the inhibitors, and competitive addition of non-protein nucleophiles to the inhibitors. The most active antivirals are slowly reversible inhibitors with elongated target residence times. The study reveals first examples for the chemical evolution of bio-actives through protein-catalyzed, non-enzymatic CC-couplings. The discovered mechanism works under physiological conditions and might constitute a native process of drug development.
2020
[163]
M. Bermudez, M. Grabowski, M. S. Murgueitio, M. Tiemann, P. Varga, T. Rudolf, G. Wolber, G. Weindl, and J. Rademann. Biological characterization, mechanistic investigation and structure-activity relationships of chemically stable TLR2 antagonists, ChemMedChem, 15(14):1364-1371, 2020.
Links:
[doi:10.1002/cmdc.202000060]
[show BibTeX]
[show abstract]
x
@article{RN284,
author = {Bermudez, M. and Grabowski, M. and
Murgueitio, M. S. and Tiemann, M. and
Varga, P. and Rudolf, T. and Wolber, G.
and Weindl, G. and Rademann, J.},
title = {Biological characterization, mechanistic
investigation and structure-activity
relationships of chemically stable TLR2
antagonists},
journal = {Chemmedchem},
volume = {15},
number = {14},
pages = {1364-1371},
note = {Mm1dj Times Cited:0 Cited References
Count:34},
abstract = {Toll-like receptors (TLRs) build the
first barrier in the innate immune
response and therefore represent promising
targets for the modulation of inflammatory
processes. Recently, the
pyrogallol-containing TLR2 antagonists
CU-CPT22 and MMG-11 were reported;
however, their 1,2,3-triphenol motif
renders them highly susceptible to
oxidation and excludes them from use in
extended experiments under aerobic
conditions. Therefore, we have developed a
set of novel TLR2 antagonists (1-9) based
on the systematic variation of
substructures, linker elements, and the
hydrogen-bonding pattern of the pyrogallol
precursors by using chemically robust
building blocks. The novel series of
chemically stable and synthetically
accessible TLR2 antagonists (1-9) was
pharmacologically characterized, and the
potential binding modes of the active
compounds were evaluated structurally. Our
results provide new insights into
structure-activity relationships and allow
rationalization of structural binding
characteristics. Moreover, they support
the hypothesis that this class of TLR
ligands bind solely to TLR2 and do not
directly interact with TLR1 or TLR6 of the
functional heterodimer. The most active
compound from this series (6), is
chemically stable, nontoxic,
TLR2-selective, and shows a similar
activity with regard to the pyrogallol
starting points, thus indicating the
variability of the hydrogen bonding
pattern.},
keywords = {chemical synthesis inflammation molecular
modeling structure-based design tlr
selectivity toll-like receptors
pattern-recognition receptors discovery
peptides agonists design},
ISSN = {1860-7179},
DOI = {10.1002/cmdc.202000060},
url = {Go to ISI://WOS:000537253200001
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496872/pdf/CMDC-15-1364.pdf},
year = {2020},
type = {Journal Article}
}
x
Biological characterization, mechanistic investigation and structure-activity relationships of chemically stable TLR2 antagonists
Toll-like receptors (TLRs) build the first barrier in the innate immune response and therefore represent promising targets for the modulation of inflammatory processes. Recently, the pyrogallol-containing TLR2 antagonists CU-CPT22 and MMG-11 were reported; however, their 1,2,3-triphenol motif renders them highly susceptible to oxidation and excludes them from use in extended experiments under aerobic conditions. Therefore, we have developed a set of novel TLR2 antagonists (1-9) based on the systematic variation of substructures, linker elements, and the hydrogen-bonding pattern of the pyrogallol precursors by using chemically robust building blocks. The novel series of chemically stable and synthetically accessible TLR2 antagonists (1-9) was pharmacologically characterized, and the potential binding modes of the active compounds were evaluated structurally. Our results provide new insights into structure-activity relationships and allow rationalization of structural binding characteristics. Moreover, they support the hypothesis that this class of TLR ligands bind solely to TLR2 and do not directly interact with TLR1 or TLR6 of the functional heterodimer. The most active compound from this series (6), is chemically stable, nontoxic, TLR2-selective, and shows a similar activity with regard to the pyrogallol starting points, thus indicating the variability of the hydrogen bonding pattern.
K. Denzinger, T. N. Nguyen, T. Noonan, G. Wolber, and M. Bermudez. Biased ligands differentially shape the conformation of the extracellular loop region in 5-HT2B receptors, Int J Mol Sci, 21(24):9728, 2020.
Links:
[doi:10.3390/ijms21249728]
[show BibTeX]
[show abstract]
x
@article{RN301,
author = {Denzinger, Katrin and Nguyen, Trung Ngoc
and Noonan, Theresa and Wolber, Gerhard
and Bermudez, Marcel},
title = {Biased ligands differentially shape the
conformation of the extracellular loop
region in 5-HT2B receptors},
journal = {International Journal of Molecular
Sciences},
volume = {21},
number = {24},
pages = {9728},
note = {Denzinger, Katrin Nguyen, Trung Ngoc
Noonan, Theresa Wolber, Gerhard Bermudez,
Marcel eng 407626949/Deutsche
Forschungsgemeinschaft Switzerland Int J
Mol Sci. 2020 Dec 20;21(24). pii:
ijms21249728. doi: 10.3390/ijms21249728.},
abstract = {G protein-coupled receptors are linked to
various intracellular transducers, each
pathway associated with different
physiological effects. Biased ligands,
capable of activating one pathway over
another, are gaining attention for their
therapeutic potential, as they could
selectively activate beneficial pathways
whilst avoiding those responsible for
adverse effects. We performed molecular
dynamics simulations with known
beta-arrestin-biased ligands like lysergic
acid diethylamide and ergotamine in
complex with the 5-HT2B receptor and
discovered that the extent of ligand bias
is directly connected with the degree of
closure of the extracellular loop region.
Given a loose allosteric coupling of
extracellular and intracellular receptor
regions, we delineate a concept for biased
signaling at serotonin receptors, by which
conformational interference with binding
pocket closure restricts the signaling
repertoire of the receptor. Molecular
docking studies of biased ligands gathered
from the BiasDB demonstrate that larger
ligands only show plausible docking poses
in the ergotamine-bound structure,
highlighting the conformational
constraints associated with bias. This
emphasizes the importance of selecting the
appropriate receptor conformation on which
to base virtual screening workflows in
structure-based drug design of biased
ligands. As this mechanism of ligand bias
has also been observed for muscarinic
receptors, our studies provide a general
mechanism of signaling bias transferable
between aminergic receptors.},
keywords = {Gpcr biased signaling conformational
descriptor drug design molecular dynamics
pharmacophore serotonin receptors virtual
screening},
ISSN = {1422-0067},
DOI = {10.3390/ijms21249728},
url = {https://www.mdpi.com/1422-0067/21/24/9728
https://res.mdpi.com/d_attachment/ijms/ijms-21-09728/article_deploy/ijms-21-09728.pdf},
year = {2020},
type = {Journal Article}
}
x
Biased ligands differentially shape the conformation of the extracellular loop region in 5-HT2B receptors
G protein-coupled receptors are linked to various intracellular transducers, each pathway associated with different physiological effects. Biased ligands, capable of activating one pathway over another, are gaining attention for their therapeutic potential, as they could selectively activate beneficial pathways whilst avoiding those responsible for adverse effects. We performed molecular dynamics simulations with known beta-arrestin-biased ligands like lysergic acid diethylamide and ergotamine in complex with the 5-HT2B receptor and discovered that the extent of ligand bias is directly connected with the degree of closure of the extracellular loop region. Given a loose allosteric coupling of extracellular and intracellular receptor regions, we delineate a concept for biased signaling at serotonin receptors, by which conformational interference with binding pocket closure restricts the signaling repertoire of the receptor. Molecular docking studies of biased ligands gathered from the BiasDB demonstrate that larger ligands only show plausible docking poses in the ergotamine-bound structure, highlighting the conformational constraints associated with bias. This emphasizes the importance of selecting the appropriate receptor conformation on which to base virtual screening workflows in structure-based drug design of biased ligands. As this mechanism of ligand bias has also been observed for muscarinic receptors, our studies provide a general mechanism of signaling bias transferable between aminergic receptors.
W. Du, D. Machalz, Q. Yan, E. J. Sorensen, G. Wolber, and M. Bureik. Importance of asparagine-381 and arginine-487 for substrate recognition in CYP4Z1, Biochem Pharmacol, 174:113850, 2020.
Links:
[doi:10.1016/j.bcp.2020.113850]
[show BibTeX]
[show abstract]
x
@article{RN280,
author = {Du, Wei and Machalz, David and Yan, Qi
and Sorensen, Erik J. and Wolber, Gerhard
and Bureik, Matthias},
title = {Importance of asparagine-381 and
arginine-487 for substrate recognition in
CYP4Z1},
journal = {Biochemical Pharmacology},
volume = {174},
pages = {113850},
abstract = {The human cytochrome P450 enzyme CYP4Z1
remains an understudied enzyme despite its
association with poor prognosis and
overexpression in breast cancer. Hence,
CYP4Z1 has previously been suggested as an
anti-breast cancer target. In the present
study we employed extended mutation
analysis to increase our understanding of
the substrate binding mode of this enzyme.
In a combined in vitro and in silico
approach we show for the first time that
residue Arg487 plays an important role in
substrate recognition and binding of
CYP4Z1. Using a large array of recombinant
CYP4Z1 mutants we show that, apart from
Asn381, all other postulated binding
residues only play an auxiliary role in
substrate recognition and binding.
Different substrate interaction motifs
were identified via dynamic pharmacophores
(dynophores) and their impact on
catalytically competent substrate binding
was classified. These new insights on the
substrate recognition and binding mode
represent an important step towards the
rational design of CYP4Z1 prodrugs and
guide further investigations into the so
far poorly understood physiological role
of CYP4Z1.},
keywords = {Breast cancer CYP4Z1 Mutational studies
Molecular modeling Substrate recognition},
ISSN = {0006-2952},
DOI = {10.1016/j.bcp.2020.113850},
url = {http://www.sciencedirect.com/science/article/pii/S000629522030071X},
year = {2020},
type = {Journal Article}
}
x
Importance of asparagine-381 and arginine-487 for substrate recognition in CYP4Z1
The human cytochrome P450 enzyme CYP4Z1 remains an understudied enzyme despite its association with poor prognosis and overexpression in breast cancer. Hence, CYP4Z1 has previously been suggested as an anti-breast cancer target. In the present study we employed extended mutation analysis to increase our understanding of the substrate binding mode of this enzyme. In a combined in vitro and in silico approach we show for the first time that residue Arg487 plays an important role in substrate recognition and binding of CYP4Z1. Using a large array of recombinant CYP4Z1 mutants we show that, apart from Asn381, all other postulated binding residues only play an auxiliary role in substrate recognition and binding. Different substrate interaction motifs were identified via dynamic pharmacophores (dynophores) and their impact on catalytically competent substrate binding was classified. These new insights on the substrate recognition and binding mode represent an important step towards the rational design of CYP4Z1 prodrugs and guide further investigations into the so far poorly understood physiological role of CYP4Z1.
M. Dumitrascuta, M. Bermudez, S. Ballet, G. Wolber, and M. Spetea. Mechanistic understanding of peptide analogues, dalda, [Dmt1]DALDA, and KGOP01, binding to the µ opioid receptor, Molecules, 25(9):2087, 2020.
Links:
[doi:10.3390/molecules25092087]
[show BibTeX]
[show abstract]
x
@article{RN285,
author = {Dumitrascuta, M. and Bermudez, M. and
Ballet, S. and Wolber, G. and Spetea, M.},
title = {Mechanistic understanding of peptide
analogues, dalda, [Dmt1]DALDA, and KGOP01,
binding to the µ opioid receptor},
journal = {Molecules},
volume = {25},
number = {9},
pages = {2087},
note = {Lr4vz Times Cited:2 Cited References
Count:52},
abstract = {The mu opioid receptor (MOR) is the
primary target for analgesia of endogenous
opioid peptides, alkaloids, synthetic
small molecules with diverse scaffolds,
and peptidomimetics. Peptide-based opioids
are viewed as potential analgesics with
reduced side effects and have received
constant scientific interest over the
years. This study focuses on three potent
peptide and peptidomimetic MOR agonists,
DALDA, [Dmt(1)]DALDA, and KGOP01, and the
prototypical peptide MOR agonist DAMGO. We
present the first molecular modeling study
and structure-activity relationships aided
by in vitro assays and molecular docking
of the opioid peptide analogues, in order
to gain insight into their mode of binding
to the MOR. In vitro binding and
functional assays revealed the same rank
order with KGOP01 [Dmt(1)]DALDA DAMGO
DALDA for both binding and MOR
activation. Using molecular docking at the
MOR and three-dimensional interaction
pattern analysis, we have rationalized the
experimental outcomes and highlighted key
amino acid residues responsible for
agonist binding to the MOR. The Dmt (2 ',6
'-dimethyl-L-Tyr) moiety of [Dmt(1)]DALDA
and KGOP01 was found to represent the
driving force for their high potency and
agonist activity at the MOR. These
findings contribute to a deeper
understanding of MOR function and flexible
peptide ligand-MOR interactions, that are
of significant relevance for the future
design of opioid peptide-based
analgesics.},
keywords = {mu opioid receptor opioid peptides and
peptidomimetics damgo dalda [dmt(1)]dalda
kgop01 binding molecular docking
structure-activity relationships in-vitro
potent pharmacology
h-dmt-d-arg-phe-lys-nh2 activation ligands
agonist},
ISSN = {1420-3049 (Electronic) 1420-3049
(Linking)},
DOI = {10.3390/molecules25092087},
url = {Go to ISI://WOS:000535695900083
https://res.mdpi.com/d_attachment/molecules/molecules-25-02087/article_deploy/molecules-25-02087-v2.pdf},
year = {2020},
type = {Journal Article}
}
x
Mechanistic understanding of peptide analogues, dalda, [Dmt1]DALDA, and KGOP01, binding to the µ opioid receptor
The mu opioid receptor (MOR) is the primary target for analgesia of endogenous opioid peptides, alkaloids, synthetic small molecules with diverse scaffolds, and peptidomimetics. Peptide-based opioids are viewed as potential analgesics with reduced side effects and have received constant scientific interest over the years. This study focuses on three potent peptide and peptidomimetic MOR agonists, DALDA, [Dmt(1)]DALDA, and KGOP01, and the prototypical peptide MOR agonist DAMGO. We present the first molecular modeling study and structure-activity relationships aided by in vitro assays and molecular docking of the opioid peptide analogues, in order to gain insight into their mode of binding to the MOR. In vitro binding and functional assays revealed the same rank order with KGOP01 > [Dmt(1)]DALDA > DAMGO > DALDA for both binding and MOR activation. Using molecular docking at the MOR and three-dimensional interaction pattern analysis, we have rationalized the experimental outcomes and highlighted key amino acid residues responsible for agonist binding to the MOR. The Dmt (2 ',6 '-dimethyl-L-Tyr) moiety of [Dmt(1)]DALDA and KGOP01 was found to represent the driving force for their high potency and agonist activity at the MOR. These findings contribute to a deeper understanding of MOR function and flexible peptide ligand-MOR interactions, that are of significant relevance for the future design of opioid peptide-based analgesics.
M. Dumitrascuta, M. Bermudez, T. Ben Haddou, E. Guerrieri, L. Schlafer, A. Ritsch, S. Hosztafi, A. Lantero, C. Kreutz, D. Massotte, H. Schmidhammer, G. Wolber, and M. Spetea. N-phenethyl substitution in 14-methoxy-n-methylmorphinan-6-ones turns selective mu opioid receptor ligands into dual µ/δ opioid receptor agonists, Scientific Reports, 10(1):5653, 2020.
Links:
[doi:10.1038/s41598-020-62530-w]
[show BibTeX]
[show abstract]
x
@article{RN290,
author = {Dumitrascuta, M. and Bermudez, M. and Ben
Haddou, T. and Guerrieri, E. and Schlafer,
L. and Ritsch, A. and Hosztafi, S. and
Lantero, A. and Kreutz, C. and Massotte,
D. and Schmidhammer, H. and Wolber, G. and
Spetea, M.},
title = {N-phenethyl substitution in
14-methoxy-n-methylmorphinan-6-ones turns
selective mu opioid receptor ligands into
dual µ/δ opioid receptor agonists},
journal = {Scientific Reports},
volume = {10},
number = {1},
pages = {5653},
note = {Nb3it Times Cited:1 Cited References
Count:56},
abstract = {Morphine and structurally-derived
compounds are mu opioid receptor (mu OR)
agonists, and the most effective analgesic
drugs. However, their usefulness is
limited by serious side effects, including
dependence and abuse potential. The
N-substituent in morphinans plays an
important role in opioid activities in
vitro and in vivo. This study presents the
synthesis and pharmacological evaluation
of new N-phenethyl substituted
14-O-methylmorphinan-6-ones. Whereas
substitution of the N-methyl substituent
in morphine (1) and oxymorphone (2) by an
N-phenethyl group enhances binding
affinity, selectivity and agonist potency
at the mu OR of 1a and 2a, the N-phenethyl
substitution in
14-methoxy-N-methylmorphinan-6-ones (3 and
4) converts selective mu OR ligands into
dual mu/delta OR agonists (3a and 4a).
Contrary to N-methylmorphinans 1-4, the
N-phenethyl substituted morphinans 1a-4a
produce effective and potent
antinociception without motor impairment
in mice. Using docking and molecular
dynamics simulations with the mu OR, we
establish that N-methylmorphinans 1-4 and
their N-phenethyl counterparts 1a-4a share
several essential receptor-ligand
interactions, but also interaction pattern
differences related to specific structural
features, thus providing a structural
basis for their pharmacological profiles.
The emerged structure-activity
relationships in this class of morphinans
provide important information for tuning
in vitro and in vivo opioid activities
towards discovery of effective and safer
analgesics.},
keywords = {biological evaluation delta-agonists
highly potent 14-alkoxymorphinans
derivatives analgesics 14-methoxymetopon
antagonism tolerance 14-alkoxy},
ISSN = {2045-2322},
DOI = {10.1038/s41598-020-62530-w},
url = {Go to ISI://WOS:000560409100007
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7101422/pdf/41598_2020_Article_62530.pdf},
year = {2020},
type = {Journal Article}
}
x
N-phenethyl substitution in 14-methoxy-n-methylmorphinan-6-ones turns selective mu opioid receptor ligands into dual µ/δ opioid receptor agonists
Morphine and structurally-derived compounds are mu opioid receptor (mu OR) agonists, and the most effective analgesic drugs. However, their usefulness is limited by serious side effects, including dependence and abuse potential. The N-substituent in morphinans plays an important role in opioid activities in vitro and in vivo. This study presents the synthesis and pharmacological evaluation of new N-phenethyl substituted 14-O-methylmorphinan-6-ones. Whereas substitution of the N-methyl substituent in morphine (1) and oxymorphone (2) by an N-phenethyl group enhances binding affinity, selectivity and agonist potency at the mu OR of 1a and 2a, the N-phenethyl substitution in 14-methoxy-N-methylmorphinan-6-ones (3 and 4) converts selective mu OR ligands into dual mu/delta OR agonists (3a and 4a). Contrary to N-methylmorphinans 1-4, the N-phenethyl substituted morphinans 1a-4a produce effective and potent antinociception without motor impairment in mice. Using docking and molecular dynamics simulations with the mu OR, we establish that N-methylmorphinans 1-4 and their N-phenethyl counterparts 1a-4a share several essential receptor-ligand interactions, but also interaction pattern differences related to specific structural features, thus providing a structural basis for their pharmacological profiles. The emerged structure-activity relationships in this class of morphinans provide important information for tuning in vitro and in vivo opioid activities towards discovery of effective and safer analgesics.
M. T. Gabr, D. Machalz, S. Pach, and G. Wolber. A benzoxazole derivative as an inhibitor of anaerobic choline metabolism by human gut microbiota, RSC Medicinal Chemistry, 11(12):1402-1412, 2020.
Links:
[doi:10.1039/D0MD00218F]
[show BibTeX]
[show abstract]
x
@article{RN298,
author = {Gabr, Moustafa T. and Machalz, David and
Pach, Szymon and Wolber, Gerhard},
title = {A benzoxazole derivative as an inhibitor
of anaerobic choline metabolism by human
gut microbiota},
journal = {RSC Medicinal Chemistry},
volume = {11},
number = {12},
pages = {1402-1412},
note = {Pg4qx Times Cited:0 Cited References
Count:65},
abstract = {Metabolic pathways mediated by human gut
bacteria have emerged as potential
therapeutic targets because of their
association with the pathophysiology of
various human diseases. The anaerobic
transformation of choline into
trimethylamine (TMA) by gut microbiota is
directly linked to type 2 diabetes, fatty
liver disease, and cardiovascular
diseases. Structural analogs of choline
have been developed as competitive
inhibitors of choline TMA-lyase (CutC), a
key enzyme for the conversion of choline
to TMA. However, weak to moderate CutC
inhibitory profiles of the choline analogs
limit their further advancement into
clinical translation. In this study, we
introduce a glycomimetic-based approach
for the identification of CutC inhibitors
with intestinal metabolic stability. Our
workflow started with screening of a small
library of glycomimetics for metabolic
stability in the presence of human
intestinal S9 fraction. Further screening
using an in vitro CutC inhibitory assay
identified a benzoxazole ligand (BO-I) as
a CutC inhibitor with an IC50 value of 2.4
± 0.3 μM. Kinetic analysis revealed that
BO-I functions as a non-competitive
inhibitor of CutC. Interestingly, BO-I
reduced the production of TMA in whole
cell assays of multiple bacterial strains
as well as in complex biological
environments. Therefore, structural
optimization of BO-I holds promise for the
development of efficient gut
microbiota-targeted small molecules.},
keywords = {trimethylamine n-oxide drug-metabolism
monooxygenase 3 health glycomimetics
chemistry impact lyase fmo3},
DOI = {10.1039/D0MD00218F},
url = {http://dx.doi.org/10.1039/D0MD00218F
https://pubs.rsc.org/en/content/articlepdf/2020/md/d0md00218f},
year = {2020},
type = {Journal Article}
}
x
A benzoxazole derivative as an inhibitor of anaerobic choline metabolism by human gut microbiota
Metabolic pathways mediated by human gut bacteria have emerged as potential therapeutic targets because of their association with the pathophysiology of various human diseases. The anaerobic transformation of choline into trimethylamine (TMA) by gut microbiota is directly linked to type 2 diabetes, fatty liver disease, and cardiovascular diseases. Structural analogs of choline have been developed as competitive inhibitors of choline TMA-lyase (CutC), a key enzyme for the conversion of choline to TMA. However, weak to moderate CutC inhibitory profiles of the choline analogs limit their further advancement into clinical translation. In this study, we introduce a glycomimetic-based approach for the identification of CutC inhibitors with intestinal metabolic stability. Our workflow started with screening of a small library of glycomimetics for metabolic stability in the presence of human intestinal S9 fraction. Further screening using an in vitro CutC inhibitory assay identified a benzoxazole ligand (BO-I) as a CutC inhibitor with an IC50 value of 2.4 ± 0.3 μM. Kinetic analysis revealed that BO-I functions as a non-competitive inhibitor of CutC. Interestingly, BO-I reduced the production of TMA in whole cell assays of multiple bacterial strains as well as in complex biological environments. Therefore, structural optimization of BO-I holds promise for the development of efficient gut microbiota-targeted small molecules.
M. Grabowski, M. Bermudez, T. Rudolf, D. Sribar, P. Varga, M. S. Murgueitio, G. Wolber, J. Rademann, and G. Weindl. Identification and validation of a novel dual small-molecule TLR2/8 antagonist, Biochem Pharmacol, 177:113957, 2020.
Links:
[doi:10.1016/j.bcp.2020.113957]
[show BibTeX]
[show abstract]
x
@article{RN283,
author = {Grabowski, M. and Bermudez, M. and
Rudolf, T. and Sribar, D. and Varga, P.
and Murgueitio, M. S. and Wolber, G. and
Rademann, J. and Weindl, G.},
title = {Identification and validation of a novel
dual small-molecule TLR2/8 antagonist},
journal = {Biochemical Pharmacology},
volume = {177},
pages = {113957},
note = {Lz5eu Times Cited:0 Cited References
Count:61},
abstract = {Toll-like receptor 2 (TLR2) and TLR8 are
involved in the recognition of bacterial
and viral components and are linked not
only to protective antimicrobial immunity
but also to inflammatory diseases.
Recently, increasing attention has been
paid to the receptor crosstalk between
TLR2 and TLR8 to fine-tune innate immune
responses. In this study, we report a
novel dual TLR2/TLR8 antagonist, compound
24 that was developed by a modeling-guided
synthesis approach. The modulator was
optimized from the previously reported
1,3-benzothiazole derivative, compound 8.
Compound 24 was pharmacologically
characterized for the ability to inhibit
TLR2- and TLR8-mediated responses in
TLR-overexpressing reporter cells and
THP-1 macrophages. The modulator showed
high efficacy with IC50, values in the low
micromolar range for both TLR5,
selectivity towards other TLR5 and low
cytotoxicity. At TLR2, a slight
predominance for the TLR2/1 heterodimer
was found in reporter cells selectively
expressing TLR2/1 or TLR2/6 heterodimers.
Concentration ratio analysis in the
presence of Pam(3)CSK(4) or Pam(2)CSK(4)
indicated non-competitive antagonist
behavior at hTLR2. In computational
docking studies, a plausible alternative
binding mode of compound 24 was predicted
for both TLR2 and TLR8. Our results
provide evidence that it is feasible to
simultaneously and selectively target
endosomal- and surface-located TLR5. We
identified a small-molecule dual TLR2/8
antagonist that may serve as a valuable
pharmacological tool to decipher the role
of TLR2/8 co-signaling in inflammation.},
keywords = {drug discovery computer modeling
toll-like receptor (tlr) innate immunity
inflammation virtual screening dual
antagonist non-competitive antagonism
toll-like receptors human monocytes
bacterial rna human tlr8 recognition
polymorphisms cooperation inhibition
mechanisms peptides},
ISSN = {0006-2952},
DOI = {10.1016/j.bcp.2020.113957},
url = {Go to ISI://WOS:000541248000032
https://www.sciencedirect.com/science/article/abs/pii/S0006295220301854?via%3Dihub},
year = {2020},
type = {Journal Article}
}
x
Identification and validation of a novel dual small-molecule TLR2/8 antagonist
Toll-like receptor 2 (TLR2) and TLR8 are involved in the recognition of bacterial and viral components and are linked not only to protective antimicrobial immunity but also to inflammatory diseases. Recently, increasing attention has been paid to the receptor crosstalk between TLR2 and TLR8 to fine-tune innate immune responses. In this study, we report a novel dual TLR2/TLR8 antagonist, compound 24 that was developed by a modeling-guided synthesis approach. The modulator was optimized from the previously reported 1,3-benzothiazole derivative, compound 8. Compound 24 was pharmacologically characterized for the ability to inhibit TLR2- and TLR8-mediated responses in TLR-overexpressing reporter cells and THP-1 macrophages. The modulator showed high efficacy with IC50, values in the low micromolar range for both TLR5, selectivity towards other TLR5 and low cytotoxicity. At TLR2, a slight predominance for the TLR2/1 heterodimer was found in reporter cells selectively expressing TLR2/1 or TLR2/6 heterodimers. Concentration ratio analysis in the presence of Pam(3)CSK(4) or Pam(2)CSK(4) indicated non-competitive antagonist behavior at hTLR2. In computational docking studies, a plausible alternative binding mode of compound 24 was predicted for both TLR2 and TLR8. Our results provide evidence that it is feasible to simultaneously and selectively target endosomal- and surface-located TLR5. We identified a small-molecule dual TLR2/8 antagonist that may serve as a valuable pharmacological tool to decipher the role of TLR2/8 co-signaling in inflammation.
M. Grabowski, M. S. Murgueitio, M. Bermudez, G. Wolber, and G. Weindl. The novel small-molecule antagonist MMG-11 preferentially inhibits TLR2/1 signaling, Biochem Pharmacol, 171:113687, 2020.
Links:
[doi:10.1016/j.bcp.2019.113687]
[show BibTeX]
[show abstract]
x
@article{RN279,
author = {Grabowski, Maria and Murgueitio, Manuela
S. and Bermudez, Marcel and Wolber,
Gerhard and Weindl, Günther},
title = {The novel small-molecule antagonist
MMG-11 preferentially inhibits TLR2/1
signaling},
journal = {Biochemical Pharmacology},
volume = {171},
pages = {113687},
abstract = {Toll-like receptor 2 (TLR2) forms
heterodimers with either TLR1 or TLR6 to
induce protective early inflammatory
responses to pathogen- and
damage-associated molecular patterns.
However, excessive activation is
associated with inflammatory and metabolic
diseases. Several TLR2 antagonists have
been described but pharmacological
characterization is still at an early
stage. Previously, we identified the
potent and selective TLR2 antagonist
MMG-11 by computational modelling and
experimental validation. Here, we
characterized the TLR2 antagonists MMG-11
and CU-CPT22 as well as the TIR-domain
binding TLR2 antagonist C29 in
TLR-overexpressing promoter cells as well
as human and mouse macrophages. In line
with our recent studies, MMG-11 abrogated
pro-inflammatory cytokine secretion and
NF-κB activation induced by different
bacterial TLR2 agonists. MMG-11
preferentially inhibited TLR2/1 signaling
in promoter cells stably expressing TLR2
heterodimers and mouse macrophages.
Furthermore, the TLR2 antagonist blocked
ligand-induced interaction of TLR2 with
MyD88 and reduced MAP kinase and NF-κB
activation. MMG-11 and CU-CPT22 but not
C29 displaced Pam3CSK4 in an indirect
binding assay confirming the competitive
mode of action of MMG-11 and CU-CPT22.
Isobologram analysis revealed additive and
synergistic effects when the
non-competitive antagonist C29 was
combined with the competitive antagonist
MMG-11 or CU-CPT22, respectively. In
conclusion, we provide evidence that
MMG-11 acts as a competitive antagonist
with a predominance for the TLR2/1
heterodimer in human and mouse cells. Our
results also indicate that MMG-11 is a
model compound for studying TLR2
signaling.},
keywords = {Inflammation Innate immunity Toll-like
receptors TLR2 TLR2/1 heterodimer
Competitive antagonist},
ISSN = {0006-2952},
DOI = {10.1016/j.bcp.2019.113687},
year = {2020},
type = {Journal Article}
}
x
The novel small-molecule antagonist MMG-11 preferentially inhibits TLR2/1 signaling
Toll-like receptor 2 (TLR2) forms heterodimers with either TLR1 or TLR6 to induce protective early inflammatory responses to pathogen- and damage-associated molecular patterns. However, excessive activation is associated with inflammatory and metabolic diseases. Several TLR2 antagonists have been described but pharmacological characterization is still at an early stage. Previously, we identified the potent and selective TLR2 antagonist MMG-11 by computational modelling and experimental validation. Here, we characterized the TLR2 antagonists MMG-11 and CU-CPT22 as well as the TIR-domain binding TLR2 antagonist C29 in TLR-overexpressing promoter cells as well as human and mouse macrophages. In line with our recent studies, MMG-11 abrogated pro-inflammatory cytokine secretion and NF-κB activation induced by different bacterial TLR2 agonists. MMG-11 preferentially inhibited TLR2/1 signaling in promoter cells stably expressing TLR2 heterodimers and mouse macrophages. Furthermore, the TLR2 antagonist blocked ligand-induced interaction of TLR2 with MyD88 and reduced MAP kinase and NF-κB activation. MMG-11 and CU-CPT22 but not C29 displaced Pam3CSK4 in an indirect binding assay confirming the competitive mode of action of MMG-11 and CU-CPT22. Isobologram analysis revealed additive and synergistic effects when the non-competitive antagonist C29 was combined with the competitive antagonist MMG-11 or CU-CPT22, respectively. In conclusion, we provide evidence that MMG-11 acts as a competitive antagonist with a predominance for the TLR2/1 heterodimer in human and mouse cells. Our results also indicate that MMG-11 is a model compound for studying TLR2 signaling.
J. Holze, M. Bermudez, E. M. Pfeil, M. Kauk, T. Bödefeld, M. Irmen, C. Matera, C. Dallanoce, M. De Amici, U. Holzgrabe, G. M. König, C. Tränkle, G. Wolber, R. Schrage, K. Mohr, C. Hoffmann, E. Kostenis, and A. Bock. Ligand-specific allosteric coupling controls G-Protein-Coupled receptor signaling, ACS Pharmacology & Translational Science, 3(5):859-867, 2020.
Links:
[doi:10.1021/acsptsci.0c00069]
[show BibTeX]
[show abstract]
x
@article{RN296,
author = {Holze, Janine and Bermudez, Marcel and
Pfeil, Eva Marie and Kauk, Michael and
Bödefeld, Theresa and Irmen, Matthias and
Matera, Carlo and Dallanoce, Clelia and De
Amici, Marco and Holzgrabe, Ulrike and
König, Gabriele Maria and Tränkle,
Christian and Wolber, Gerhard and Schrage,
Ramona and Mohr, Klaus and Hoffmann,
Carsten and Kostenis, Evi and Bock,
Andreas},
title = {Ligand-specific allosteric coupling
controls G-Protein-Coupled receptor
signaling},
journal = {ACS Pharmacology & Translational
Science},
volume = {3},
number = {5},
pages = {859-867},
abstract = {Allosteric coupling describes a
reciprocal process whereby
G-protein-coupled receptors (GPCRs) relay
ligand-induced conformational changes from
the extracellular binding pocket to the
intracellular signaling surface.
Therefore, GPCR activation is sensitive to
both the type of extracellular ligand and
intracellular signaling protein. We
hypothesized that ligand-specific
allosteric coupling may result in
preferential (i.e., biased) engagement of
downstream effectors. However, the
structural basis underlying
ligand-dependent control of this essential
allosteric mechanism is poorly understood.
Here, we show that two sets of extended
muscarinic acetylcholine receptor M1
agonists, which only differ in linker
length, progressively constrain receptor
signaling. We demonstrate that stepwise
shortening of their chemical linker
gradually hampers binding pocket closure,
resulting in divergent coupling to
distinct G-protein families. Our data
provide an experimental strategy for the
design of ligands with selective G-protein
recognition and reveal a potentially
general mechanism of ligand-specific
allosteric coupling.},
DOI = {10.1021/acsptsci.0c00069},
url = {https://doi.org/10.1021/acsptsci.0c00069
https://pubs.acs.org/doi/pdf/10.1021/acsptsci.0c00069},
year = {2020},
type = {Journal Article}
}
x
Ligand-specific allosteric coupling controls G-Protein-Coupled receptor signaling
Allosteric coupling describes a reciprocal process whereby G-protein-coupled receptors (GPCRs) relay ligand-induced conformational changes from the extracellular binding pocket to the intracellular signaling surface. Therefore, GPCR activation is sensitive to both the type of extracellular ligand and intracellular signaling protein. We hypothesized that ligand-specific allosteric coupling may result in preferential (i.e., biased) engagement of downstream effectors. However, the structural basis underlying ligand-dependent control of this essential allosteric mechanism is poorly understood. Here, we show that two sets of extended muscarinic acetylcholine receptor M1 agonists, which only differ in linker length, progressively constrain receptor signaling. We demonstrate that stepwise shortening of their chemical linker gradually hampers binding pocket closure, resulting in divergent coupling to distinct G-protein families. Our data provide an experimental strategy for the design of ligands with selective G-protein recognition and reveal a potentially general mechanism of ligand-specific allosteric coupling.
K. B. Loboda, K. Valjavec, M. Štampar, G. Wolber, B. Žegura, M. Filipič, M. S. Dolenc, and A. Perdih. Design and synthesis of 3,5-substituted 1,2,4-oxadiazoles as catalytic inhibitors of human DNA topoisomerase IIα, Bioorg Chem, 99:103828, 2020.
Links:
[doi:10.1016/j.bioorg.2020.103828]
[show BibTeX]
[show abstract]
x
@article{RN297,
author = {Loboda, Kaja Bergant and Valjavec, Katja
and Štampar, Martina and Wolber, Gerhard
and Žegura, Bojana and Filipič, Metka
and Dolenc, Marija Sollner and Perdih,
Andrej},
title = {Design and synthesis of 3,5-substituted
1,2,4-oxadiazoles as catalytic inhibitors
of human DNA topoisomerase IIα},
journal = {Bioorganic Chemistry},
volume = {99},
pages = {103828},
abstract = {Cancer constitutes a group of diseases
linked to abnormal cell growth that can
potentially spread to other parts of the
body and is one of the most common causes
of death. The molecular motors - DNA
topoisomerases - that enable topological
changes of the DNA molecule are one of the
most established targets of cancer
therapies. Due to known limitations of
established topo II poisons such as
cardiotoxicity, induction of secondary
malignancies and recognized cancer cell
resistance, an emerging group of catalytic
topo II inhibitors attempts to circumvent
these challenges. Currently, this approach
comprises several subgroups of
mechanistically diverse inhibitors, one of
which are compounds that act by binding to
their ATPase domain. In this study we have
designed, synthesized and characterized a
new series of 3,5-substituted
1,2,4-oxadiazoles that act as catalytic
inhibitors of human topo IIα. The
introduction of the substituted rigid
substitutions on the oxadiazole backbone
was intended to enhance the interactions
with the ATP binding site. In the
inhibition assays selected compounds
revealed a new class of catalytic
inhibitors targeting this molecular motor
and showed binding to the isolated topo
IIα ATPase domain. The predicted
inhibitor binding geometries were
evaluated in molecular dynamics
simulations and subsequently dynophore
models were derived, which provided a
deeper insight into molecular recognition
with its macromolecular target. Selected
compounds also displayed in vitro
cytotoxicity on the investigated MCF-7
cancer cell line and did not induce
double-strand breaks (DSB), thus
displaying a mechanism of action diverse
from the topo II poisons also on the
cellular level. The substituted
oxadiazoles thus comprise a chemical class
of interesting compounds that are
synthetically fully amenable for further
optimization to anticancer drugs.},
keywords = {Human DNA topoisomerase IIα Catalytic
inhibitors Drug design Oxadiazoles
Anticancer agents},
ISSN = {0045-2068},
DOI = {10.1016/j.bioorg.2020.103828},
url = {http://www.sciencedirect.com/science/article/pii/S0045206820301218
https://www.sciencedirect.com/science/article/abs/pii/S0045206820301218?via%3Dihub},
year = {2020},
type = {Journal Article}
}
x
Design and synthesis of 3,5-substituted 1,2,4-oxadiazoles as catalytic inhibitors of human DNA topoisomerase IIα
Cancer constitutes a group of diseases linked to abnormal cell growth that can potentially spread to other parts of the body and is one of the most common causes of death. The molecular motors - DNA topoisomerases - that enable topological changes of the DNA molecule are one of the most established targets of cancer therapies. Due to known limitations of established topo II poisons such as cardiotoxicity, induction of secondary malignancies and recognized cancer cell resistance, an emerging group of catalytic topo II inhibitors attempts to circumvent these challenges. Currently, this approach comprises several subgroups of mechanistically diverse inhibitors, one of which are compounds that act by binding to their ATPase domain. In this study we have designed, synthesized and characterized a new series of 3,5-substituted 1,2,4-oxadiazoles that act as catalytic inhibitors of human topo IIα. The introduction of the substituted rigid substitutions on the oxadiazole backbone was intended to enhance the interactions with the ATP binding site. In the inhibition assays selected compounds revealed a new class of catalytic inhibitors targeting this molecular motor and showed binding to the isolated topo IIα ATPase domain. The predicted inhibitor binding geometries were evaluated in molecular dynamics simulations and subsequently dynophore models were derived, which provided a deeper insight into molecular recognition with its macromolecular target. Selected compounds also displayed in vitro cytotoxicity on the investigated MCF-7 cancer cell line and did not induce double-strand breaks (DSB), thus displaying a mechanism of action diverse from the topo II poisons also on the cellular level. The substituted oxadiazoles thus comprise a chemical class of interesting compounds that are synthetically fully amenable for further optimization to anticancer drugs.
S. Pach, T. M. Sarter, R. Yousef, D. Schaller, S. Bergemann, C. Arkona, J. Rademann, C. Nitsche, and G. Wolber. Catching a moving target: comparative modeling of flaviviral NS2B-NS3 reveals small molecule Zika protease inhibitors, ACS Medicinal Chemistry Letters, 11(4):514-520, 2020.
Links:
[doi:10.1021/acsmedchemlett.9b00629]
[show BibTeX]
[show abstract]
x
@article{RN287,
author = {Pach, S. and Sarter, T. M. and Yousef, R.
and Schaller, D. and Bergemann, S. and
Arkona, C. and Rademann, J. and Nitsche,
C. and Wolber, G.},
title = {Catching a moving target: comparative
modeling of flaviviral NS2B-NS3 reveals
small molecule Zika protease inhibitors},
journal = {ACS Medicinal Chemistry Letters},
volume = {11},
number = {4},
pages = {514-520},
note = {Le0gi Times Cited:1 Cited References
Count:48},
abstract = {The pivotal role of viral proteases in
virus replication has already been
successfully exploited in several
antiviral drug design campaigns. However,
no efficient antivirals are currently
available against flaviviral infections.
In this study, we present lead-like small
molecule inhibitors of the Zika Virus
(ZIKV) NS2B-NS3 protease. Since only few
nonpeptide competitive ligands are known,
we take advantage of the high structural
similarity with the West Nile Virus (WNV)
NS2B-NS3 protease. A comparative modeling
approach involving our in-house software
PyRod was employed to systematically
analyze the binding sites and develop
molecular dynamics-based 3D pharmacophores
for virtual screening. The identified
compounds were biochemically characterized
revealing low micromolar affinity for both
ZIKV and WNV proteases. Their lead-like
properties together with rationalized
binding modes represent valuable starting
points for future lead optimization. Since
the NS2B-NS3 protease is highly conserved
among flaviviruses, these compounds may
also drive the development of
pan-flaviviral antiviral drugs.},
keywords = {flavivirus protease inhibitors pyrod 3d
pharmacophores dynophores virus ns3
protease serine-protease in-vitro dengue
identification vaccine mutagenesis
discovery progress ligands},
ISSN = {1948-5875},
DOI = {10.1021/acsmedchemlett.9b00629},
url = {Go to ISI://WOS:000526402700018
https://pubs.acs.org/doi/pdf/10.1021/acsmedchemlett.9b00629},
year = {2020},
type = {Journal Article}
}
x
Catching a moving target: comparative modeling of flaviviral NS2B-NS3 reveals small molecule Zika protease inhibitors
The pivotal role of viral proteases in virus replication has already been successfully exploited in several antiviral drug design campaigns. However, no efficient antivirals are currently available against flaviviral infections. In this study, we present lead-like small molecule inhibitors of the Zika Virus (ZIKV) NS2B-NS3 protease. Since only few nonpeptide competitive ligands are known, we take advantage of the high structural similarity with the West Nile Virus (WNV) NS2B-NS3 protease. A comparative modeling approach involving our in-house software PyRod was employed to systematically analyze the binding sites and develop molecular dynamics-based 3D pharmacophores for virtual screening. The identified compounds were biochemically characterized revealing low micromolar affinity for both ZIKV and WNV proteases. Their lead-like properties together with rationalized binding modes represent valuable starting points for future lead optimization. Since the NS2B-NS3 protease is highly conserved among flaviviruses, these compounds may also drive the development of pan-flaviviral antiviral drugs.
D. Schaller, D. Sribar, T. Noonan, L. H. Deng, T. N. Nguyen, S. Pach, D. Machalz, M. Bermudez, and G. Wolber. Next generation 3D pharmacophore modeling, Wiley Interdisciplinary Reviews: Computational Molecular Science, 10(4):e1468, 2020.
Links:
[doi:10.1002/wcms.1468]
[show BibTeX]
[show abstract]
x
@article{RN291,
author = {Schaller, D. and Sribar, D. and Noonan,
T. and Deng, L. H. and Nguyen, T. N. and
Pach, S. and Machalz, D. and Bermudez, M.
and Wolber, G.},
title = {Next generation 3D pharmacophore
modeling},
journal = {Wiley Interdisciplinary Reviews:
Computational Molecular Science},
volume = {10},
number = {4},
pages = {e1468},
note = {Mc0lp Times Cited:4 Cited References
Count:111},
abstract = {3D pharmacophore models are
three-dimensional ensembles of chemically
defined interactions of a ligand in its
bioactive conformation. They represent an
elegant way to decipher chemically encoded
ligand information and have therefore
become a valuable tool in drug design. In
this review, we provide an overview on the
basic concept of this method and summarize
key studies for applying 3D pharmacophore
models in virtual screening and
mechanistic studies for protein
functionality. Moreover, we discuss recent
developments in the field. The combination
of 3D pharmacophore models with molecular
dynamics simulations could be a quantum
leap forward since these approaches
consider macromolecule-ligand interactions
as dynamic and therefore show a
physiologically relevant interaction
pattern. Other trends include the
efficient usage of 3D pharmacophore
information in machine learning and
artificial intelligence applications or
freely accessible web servers for 3D
pharmacophore modeling. The recent
developments show that 3D pharmacophore
modeling is a vibrant field with various
applications in drug discovery and beyond.
This article is categorized under:
Computer and Information Science
Chemoinformatics Computer and Information
Science Computer Algorithms and
Programming Molecular and Statistical
Mechanics Molecular Interactions},
keywords = {3d pharmacophores artifical intelligence
machine learning virtual screening web
services competitive saturation silcs drug
discovery dynamics simulations
site-identification covalent inhibitors
chemical space protein binding design
receptor},
ISSN = {1759-0876},
DOI = {10.1002/wcms.1468},
url = {Go to ISI://WOS:000516132600001
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/wcms.1468?download= true},
year = {2020},
type = {Journal Article}
}
x
Next generation 3D pharmacophore modeling
3D pharmacophore models are three-dimensional ensembles of chemically defined interactions of a ligand in its bioactive conformation. They represent an elegant way to decipher chemically encoded ligand information and have therefore become a valuable tool in drug design. In this review, we provide an overview on the basic concept of this method and summarize key studies for applying 3D pharmacophore models in virtual screening and mechanistic studies for protein functionality. Moreover, we discuss recent developments in the field. The combination of 3D pharmacophore models with molecular dynamics simulations could be a quantum leap forward since these approaches consider macromolecule-ligand interactions as dynamic and therefore show a physiologically relevant interaction pattern. Other trends include the efficient usage of 3D pharmacophore information in machine learning and artificial intelligence applications or freely accessible web servers for 3D pharmacophore modeling. The recent developments show that 3D pharmacophore modeling is a vibrant field with various applications in drug discovery and beyond. This article is categorized under: Computer and Information Science > Chemoinformatics Computer and Information Science > Computer Algorithms and Programming Molecular and Statistical Mechanics > Molecular Interactions
D. Schaller, and G. Wolber. Pyrod enables rational homology model-based virtual screening against MCHR1, Mol Inf, 39(6):e2000020, 2020.
Links:
[doi:10.1002/minf.202000020]
[show BibTeX]
[show abstract]
x
@article{RN286,
author = {Schaller, D. and Wolber, G.},
title = {Pyrod enables rational homology
model-based virtual screening against
MCHR1},
journal = {Molecular Informatics},
volume = {39},
number = {6},
pages = {e2000020},
note = {Lv0tp Times Cited:0 Cited References
Count:40},
abstract = {Several encouraging pre-clinical results
highlight the melanin-concentrating
hormone receptor 1 (MCHR1) as promising
target for anti-obesity drug development.
Currently however, experimentally resolved
structures of MCHR1 are not available,
which complicates rational drug design
campaigns. In this study, we aimed at
developing accurate, homologymodel-based
3D pharmacophores against MCHR1. We show
that traditional approaches involving
docking of known active small molecules
are hindered by the flexibility of binding
pocket residues. Instead, we derived
three-dimensional pharmacophores from
molecular dynamics simulations by
employing our novel open-source software
PyRod. In a retrospective evaluation, the
generated 3D pharmacophores were highly
predictive returning up to 35 % of active
molecules and showing an early enrichment
(EF1) of up to 27.6. Furthermore, PyRod
pharmacophores demonstrate higher
sensitivity than ligand-based
pharmacophores and deliver structural
insights, which are key to rational lead
optimization.},
keywords = {mchr1 pyrod 3d pharmacophore homology
modeling md simulation protein dynamics
database ligands system diet},
ISSN = {1868-1743},
DOI = {10.1002/minf.202000020},
url = {Go to ISI://WOS:000529246800001
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317519/pdf/MINF-39-2000020.pdf},
year = {2020},
type = {Journal Article}
}
x
Pyrod enables rational homology model-based virtual screening against MCHR1
Several encouraging pre-clinical results highlight the melanin-concentrating hormone receptor 1 (MCHR1) as promising target for anti-obesity drug development. Currently however, experimentally resolved structures of MCHR1 are not available, which complicates rational drug design campaigns. In this study, we aimed at developing accurate, homologymodel-based 3D pharmacophores against MCHR1. We show that traditional approaches involving docking of known active small molecules are hindered by the flexibility of binding pocket residues. Instead, we derived three-dimensional pharmacophores from molecular dynamics simulations by employing our novel open-source software PyRod. In a retrospective evaluation, the generated 3D pharmacophores were highly predictive returning up to 35 % of active molecules and showing an early enrichment (EF1) of up to 27.6. Furthermore, PyRod pharmacophores demonstrate higher sensitivity than ligand-based pharmacophores and deliver structural insights, which are key to rational lead optimization.
D. Stepanov, S. Canipa, and G. Wolber. HuskinDB, a database for skin permeation of xenobiotics, Scientific Data, 7(1):426, 2020.
Links:
[doi:10.1038/s41597-020-00764-z]
[show BibTeX]
[show abstract]
x
@article{RN294,
author = {Stepanov, Dmitri and Canipa, Steven and
Wolber, Gerhard},
title = {HuskinDB, a database for skin permeation
of xenobiotics},
journal = {Scientific Data},
volume = {7},
number = {1},
pages = {426},
abstract = {Skin permeation is an essential
biological property of small organic
compounds our body is exposed to, such as
drugs in topic formulations, cosmetics,
and environmental toxins. Despite the
limited availability of experimental data,
there is a lack of systematic analysis and
structure. We present a novel resource on
skin permeation data that collects all
measurements available in the literature
and systematically structures experimental
conditions. Besides the skin permeation
value kp, it includes experimental
protocols such as skin source site, skin
layer used, preparation technique, storage
conditions, as well as test conditions
such as temperature, pH as well as the
type of donor and acceptor solution. It is
important to include these parameters in
the assessment of the skin permeation
data. In addition, we provide an analysis
of physicochemical properties and chemical
space coverage, laying the basis for
applicability domain determination of
insights drawn from the collected data
points. The database is freely accessible
under https://huskindb.drug-design.deor
https://doi.org/10.7303/syn21998881.},
ISSN = {2052-4463},
DOI = {10.1038/s41597-020-00764-z},
url = {https://doi.org/10.1038/s41597-020-00764-z
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708619/pdf/41597_2020_Article_764.pdf},
year = {2020},
type = {Journal Article}
}
x
HuskinDB, a database for skin permeation of xenobiotics
Skin permeation is an essential biological property of small organic compounds our body is exposed to, such as drugs in topic formulations, cosmetics, and environmental toxins. Despite the limited availability of experimental data, there is a lack of systematic analysis and structure. We present a novel resource on skin permeation data that collects all measurements available in the literature and systematically structures experimental conditions. Besides the skin permeation value kp, it includes experimental protocols such as skin source site, skin layer used, preparation technique, storage conditions, as well as test conditions such as temperature, pH as well as the type of donor and acceptor solution. It is important to include these parameters in the assessment of the skin permeation data. In addition, we provide an analysis of physicochemical properties and chemical space coverage, laying the basis for applicability domain determination of insights drawn from the collected data points. The database is freely accessible under https://huskindb.drug-design.deor https://doi.org/10.7303/syn21998881.
Y. Sun, D. Machalz, G. Wolber, M. K. Parr, and M. Bureik. Functional expression of all human sulfotransferases in fission yeast, assay development, and structural models for isoforms SULT4A1 and SULT6B1, Biomolecules, 10(11):1517, 2020.
Links:
[doi:10.3390/biom10111517]
[show BibTeX]
x
@article{RN295,
author = {Sun, Yanan and Machalz, David and Wolber,
Gerhard and Parr, Maria Kristina and
Bureik, Matthias},
title = {Functional expression of all human
sulfotransferases in fission yeast, assay
development, and structural models for
isoforms SULT4A1 and SULT6B1},
journal = {Biomolecules},
volume = {10},
number = {11},
pages = {1517},
ISSN = {2218-273X},
DOI = {10.3390/biom10111517},
url = {https://www.mdpi.com/2218-273X/10/11/1517
https://res.mdpi.com/d_attachment/biomolecules/biomolecules-10-01517/article_deploy/biomolecules-10-01517.pdf},
year = {2020},
type = {Journal Article}
}
D. Volpato, M. Kauk, R. Messerer, M. Bermudez, G. Wolber, A. Bock, C. Hoffmann, and U. Holzgrabe. The role of orthosteric building blocks of bitopic ligands for muscarinic M1 receptors, ACS Omega, 5(49):31706-31715, 2020.
Links:
[doi:10.1021/acsomega.0c04220]
[show BibTeX]
[show abstract]
x
@article{RN300,
author = {Volpato, Daniela and Kauk, Michael and
Messerer, Regina and Bermudez, Marcel and
Wolber, Gerhard and Bock, Andreas and
Hoffmann, Carsten and Holzgrabe, Ulrike},
title = {The role of orthosteric building blocks
of bitopic ligands for muscarinic M1
receptors},
journal = {ACS Omega},
volume = {5},
number = {49},
pages = {31706-31715},
abstract = {The muscarinic M1 acetylcholine receptor
is an important drug target for the
treatment of various neurological
disorders. Designing M1 receptor-selective
drugs has proven challenging, mainly due
to the high conservation of the
acetylcholine binding site among
muscarinic receptor subtypes. Therefore,
less conserved and topographically
distinct allosteric binding sites have
been explored to increase M1 receptor
selectivity. In this line, bitopic
ligands, which target orthosteric and
allosteric binding sites simultaneously,
may provide a promising strategy. Here, we
explore the allosteric, M1-selective BQCAd
scaffold derived from BQCA as a starting
point for the design, synthesis, and
pharmacological evaluation of a series of
novel bitopic ligands in which the
orthosteric moieties and linker lengths
are systematically varied. Since
β-arrestin recruitment seems to be
favorable to therapeutic implication, all
the compounds were investigated by G
protein and β-arrestin assays. Some
bitopic ligands are partial to full
agonists for G protein activation, some
activate β-arrestin recruitment, and the
degree of β-arrestin recruitment varies
according to the respective modification.
The allosteric BQCAd scaffold controls the
positioning of the orthosteric ammonium
group of all ligands, suggesting that this
interaction is essential for stimulating G
protein activation. However, β-arrestin
recruitment is not affected. The novel set
of bitopic ligands may constitute a
toolbox to study the requirements of
β-arrestin recruitment during ligand
design for therapeutic usage.},
ISSN = {2470-1343},
DOI = {10.1021/acsomega.0c04220},
url = {https://doi.org/10.1021/acsomega.0c04220
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7745449/pdf/ao0c04220.pdf},
year = {2020},
type = {Journal Article}
}
x
The role of orthosteric building blocks of bitopic ligands for muscarinic M1 receptors
The muscarinic M1 acetylcholine receptor is an important drug target for the treatment of various neurological disorders. Designing M1 receptor-selective drugs has proven challenging, mainly due to the high conservation of the acetylcholine binding site among muscarinic receptor subtypes. Therefore, less conserved and topographically distinct allosteric binding sites have been explored to increase M1 receptor selectivity. In this line, bitopic ligands, which target orthosteric and allosteric binding sites simultaneously, may provide a promising strategy. Here, we explore the allosteric, M1-selective BQCAd scaffold derived from BQCA as a starting point for the design, synthesis, and pharmacological evaluation of a series of novel bitopic ligands in which the orthosteric moieties and linker lengths are systematically varied. Since β-arrestin recruitment seems to be favorable to therapeutic implication, all the compounds were investigated by G protein and β-arrestin assays. Some bitopic ligands are partial to full agonists for G protein activation, some activate β-arrestin recruitment, and the degree of β-arrestin recruitment varies according to the respective modification. The allosteric BQCAd scaffold controls the positioning of the orthosteric ammonium group of all ligands, suggesting that this interaction is essential for stimulating G protein activation. However, β-arrestin recruitment is not affected. The novel set of bitopic ligands may constitute a toolbox to study the requirements of β-arrestin recruitment during ligand design for therapeutic usage.
2019 and earlier
[147]
L. Agnetta, M. Bermudez, F. Riefolo, C. Matera, E. Claro, R. Messerer, T. Littmann, G. Wolber, U. Holzgrabe, and M. Decker. Fluorination of photoswitchable muscarinic agonists tunes receptor pharmacology and photochromic properties, J Med Chem, 62(6):3009-3020, 2019.
Links:
[doi:10.1021/acs.jmedchem.8b01822]
[show BibTeX]
x
@article{RN264,
author = {Agnetta, Luca and Bermudez, Marcel and
Riefolo, Fabio and Matera, Carlo and
Claro, Enrique and Messerer, Regina and
Littmann, Timo and Wolber, Gerhard and
Holzgrabe, Ulrike and Decker, Michael},
title = {Fluorination of photoswitchable
muscarinic agonists tunes receptor
pharmacology and photochromic properties},
journal = {Journal of Medicinal Chemistry},
volume = {62},
number = {6},
pages = {3009-3020},
keywords = {gpcr},
ISSN = {0022-2623},
DOI = {10.1021/acs.jmedchem.8b01822},
url = {https://doi.org/10.1021/acs.jmedchem.8b01822
https://pubs.acs.org/doi/pdfplus/10.1021/acs.jmedchem.8b01822},
year = {2019},
type = {Journal Article}
}
H. Aygun Cevher, D. Schaller, M. A. Gandini, O. Kaplan, E. Gambeta, F. X. Zhang, M. Celebier, M. N. Tahir, G. W. Zamponi, G. Wolber, and M. G. Gunduz. Discovery of Michael acceptor containing 1,4-dihydropyridines as first covalent inhibitors of L-/T-type calcium channels, Bioorg Chem, 91:103187, 2019.
Links:
[doi:10.1016/j.bioorg.2019.103187]
[show BibTeX]
[show abstract]
x
@article{RN276,
author = {Aygun Cevher, H. and Schaller, D. and
Gandini, M. A. and Kaplan, O. and Gambeta,
E. and Zhang, F. X. and Celebier, M. and
Tahir, M. N. and Zamponi, G. W. and
Wolber, G. and Gunduz, M. G.},
title = {Discovery of Michael acceptor containing
1,4-dihydropyridines as first covalent
inhibitors of L-/T-type calcium channels},
journal = {Bioorg Chem},
volume = {91},
pages = {103187},
note = {Aygun Cevher, Hande Schaller, David
Gandini, Maria A Kaplan, Ozan Gambeta,
Eder Zhang, Fang Xiong Celebier, Mustafa
Tahir, Muhammad Nawaz Zamponi, Gerald W
Wolber, Gerhard Gunduz, Miyase Gozde eng
Bioorg Chem. 2019 Aug 7;91:103187. doi:
10.1016/j.bioorg.2019.103187.},
abstract = {1,4-Dihydropyridines (DHPs) are an
important class of blockers targeting
different calcium channel subtypes and
have great therapeutic value against
cardiovascular and neurophysiologic
conditions. Here, we present the design of
DHP-based hexahydroquinoline derivatives
as either selective or covalent inhibitors
of calcium channels. These compounds were
synthesized via a modified Hantzsch
reaction under microwave irradiation and
characterized by IR, (1)H NMR, (13)C NMR
and mass spectra. Additionally, the
proposed structure of HM12 was resolved by
single crystal X-ray analysis. The
abilities of the target compounds to block
both L- and T-type calcium channels were
evaluated by utilizing the whole-cell
patch clamp technique. Our results
identified covalent inhibitors of calcium
channels for the first time, which could
be achieved by introducing a Michael
acceptor group into the ester side chain
of the compounds. The proposed covalent
binding between the compounds and the
cysteine amino acid (Cys1492) within the
DHP binding pocket of L-type calcium
channel was supported by docking and
pharmacophore analysis as well as a
glutathione reactivity assay.},
keywords = {Calcium channel blocker Covalent binding
Dihydropyridine Hexahydroquinoline
Molecular modeling Whole-cell patch
clamp},
ISSN = {1090-2120 (Electronic) 0045-2068
(Linking)},
DOI = {10.1016/j.bioorg.2019.103187},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31419643
https://www.sciencedirect.com/science/article/abs/pii/S0045206819309290?via%3Dihub},
year = {2019},
type = {Journal Article}
}
x
Discovery of Michael acceptor containing 1,4-dihydropyridines as first covalent inhibitors of L-/T-type calcium channels
1,4-Dihydropyridines (DHPs) are an important class of blockers targeting different calcium channel subtypes and have great therapeutic value against cardiovascular and neurophysiologic conditions. Here, we present the design of DHP-based hexahydroquinoline derivatives as either selective or covalent inhibitors of calcium channels. These compounds were synthesized via a modified Hantzsch reaction under microwave irradiation and characterized by IR, (1)H NMR, (13)C NMR and mass spectra. Additionally, the proposed structure of HM12 was resolved by single crystal X-ray analysis. The abilities of the target compounds to block both L- and T-type calcium channels were evaluated by utilizing the whole-cell patch clamp technique. Our results identified covalent inhibitors of calcium channels for the first time, which could be achieved by introducing a Michael acceptor group into the ester side chain of the compounds. The proposed covalent binding between the compounds and the cysteine amino acid (Cys1492) within the DHP binding pocket of L-type calcium channel was supported by docking and pharmacophore analysis as well as a glutathione reactivity assay.
M. Bermudez, T. N. Nguyen, C. Omieczynski, and G. Wolber. Strategies for the discovery of biased GPCR ligands, Drug Discov Today, 24(4):1031-1037, 2019.
Links:
[doi:10.1016/j.drudis.2019.02.010]
[show BibTeX]
[show abstract]
x
@article{RN267,
author = {Bermudez, Marcel and Nguyen, Trung Ngoc
and Omieczynski, Christian and Wolber,
Gerhard},
title = {Strategies for the discovery of biased
GPCR ligands},
journal = {Drug Discovery Today},
volume = {24},
number = {4},
pages = {1031-1037},
abstract = {G-protein-coupled receptors (GPCRs)
represent important drug targets with
complex pharmacological characteristics.
Biased signaling represents one important
dimension, describing ligand-dependent
shifts of naturally imprinted signaling
profiles. Because biased GPCR modulators
provide potential therapeutic benefits
including higher efficiencies and reduced
adverse effects, the identification of
such ligands as drug candidates is highly
desirable. This review aims to provide an
overview of the challenges and strategies
in the discovery of biased ligands. We
show different approaches for biased
ligand discovery in the example of
G-protein-biased opioid analgesics and
discuss possibilities to design biased
ligands by targeting extracellular
receptor regions.},
keywords = {gpcr},
ISSN = {1359-6446},
DOI = {10.1016/j.drudis.2019.02.010},
url = {http://www.sciencedirect.com/science/article/pii/S1359644618302186
https://pdf.sciencedirectassets.com/271275/1-s2.0-S1359644619X00057/1-s2.0-S1359644618302186/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJGMEQCIBSuXR284U5c9bmAJSN5zVw47n5M5VTNwrBmGQ8hLXVcAiAWIgoCDAYq%2BrLMnoeOj0gcwMsJ%2BK2%2F2vx37jlwfG9V%2FiraAwhBEAIaDDA1OTAwMzU0Njg2NSIMjEBFMn9jIE9t1XN2KrcDLe7GRG%2FXcw3WEgKV9YXbSGYcHYB2z7r%2B9eRerOT0zpE0wRSFgQ%2FOH9BXlp37MIlzoDaMcVajFKZS8gcJXSICZZRdMHztkVCBW4DNZa%2BJxOwJ1E6Q6vzyyBGsc%2FRoV6Xba79A94ndoN8bYy6WNuplWyrl3mWsK2vv7sI6kqAQYvwZAuSo66P%2FLefOdgACsLXUvDUtMMpSN8TmoE6MAFh%2Fj9u1tN2PHd7Si9U0lR%2BPybWjyQIZhRLaPFiPA459dq%2F0DJYDW6PUXjnBlCqj2pjkDd8G%2FcrPGVCvjIs1dEukqNYRPh9wSQFFAb5Fi0wZV3zG%2F46Hc%2FeUQIprBOrsYtxFvQ1UWxSkaVfuuRD2NfJJC%2FoyWTyuxNmD0bdhukh9A21%2B1W83wJfu%2FecDVcxhN9MqXTDLroqrvl%2BRxWFnb%2BWbs%2B%2FV9bgL2cBI6AFt4X60UAEM%2FPSuYh%2FCMbaK1dtKiQLD4N%2F7kxQrpF%2BWzb6z4YOF5hW%2F4Jm%2BgH%2B0v9v9lego%2FcMGf98VdcRdShnMV0057mzmxPdKDL4AivAyKRRfnHrHbLoO1SdPlc41DKXR%2BjK0GtocjZ5j4aUAxDDnpo7rBTq1AThXMgqWLXhCysjiDb69KUDsmtirRuTrXpezLGPDfxkFp5ofIqWUR9J0yQNBLS%2BqlghqmfiGup4DJm90ED%2Bjr269Tuc2Ri4J0zfr7%2BcI1xixL%2Fy0rAQI30TJNbf1CsDGxfVixVDBG1Fkwa3KlpjjfAGAuGKaiPkPOMJuLo8YTbs28PVbbZSstkpcNX0npq661wJ6SM3c0BNDLzt2HCCzsKCoWT8MzDtnFnmY8Er2Z7ayg87lxx0%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090852Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYZNRZYJFV%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=7f58eff199562319314a001e9580c09f4398c03f423e7fcb0c77abbe9f6e7b3f&hash=2eec85bc780a8441b8c56fa4d0c26581dfbc0117ea28d1b8a83af57b9cc39829&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S1359644618302186&tid=spdf-e17f4a4a-0e0c-4d81-94e3-c8ec97d2a8a8&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2019},
type = {Journal Article}
}
x
Strategies for the discovery of biased GPCR ligands
G-protein-coupled receptors (GPCRs) represent important drug targets with complex pharmacological characteristics. Biased signaling represents one important dimension, describing ligand-dependent shifts of naturally imprinted signaling profiles. Because biased GPCR modulators provide potential therapeutic benefits including higher efficiencies and reduced adverse effects, the identification of such ligands as drug candidates is highly desirable. This review aims to provide an overview of the challenges and strategies in the discovery of biased ligands. We show different approaches for biased ligand discovery in the example of G-protein-biased opioid analgesics and discuss possibilities to design biased ligands by targeting extracellular receptor regions.
P. Durairaj, L. Fan, D. Machalz, G. Wolber, and M. Bureik. Functional characterization and mechanistic modeling of the human cytochrome P450 enzyme CYP4A22, Febs Lett, 593(16):2214-2225, 2019.
Links:
[doi:10.1002/1873-3468.13489]
[show BibTeX]
[show abstract]
x
@article{RN275,
author = {Durairaj, Pradeepraj and Fan, Linbing and
Machalz, David and Wolber, Gerhard and
Bureik, Matthias},
title = {Functional characterization and
mechanistic modeling of the human
cytochrome P450 enzyme CYP4A22},
journal = {FEBS Letters},
volume = {593},
number = {16},
pages = {2214-2225},
abstract = {The human cytochrome P450 (CYP) enzyme
CYP4A22 is an orphan CYP with unknown
function. Here, through functional
expression in fission yeast, we show that
CYP4A22 catalyzes fatty acid hydroxylation
as well as aliphatic or aromatic
hydroxylations of luciferin-based probe
substrates. Mechanistic molecular modeling
of CYP4A22 suggests that its
ω-hydroxylation activity is hampered by a
more spacious active site compared to
CYP4B1. Substrate recognition via
side-chains R96 and R233 is indicated by
dynamic three-dimensional pharmacophores
(dynophores) derived from molecular
dynamics simulations. CYP4A22 activity is
inhibited by three unspecific CYP
inhibitors. A comparison of CYP4A22*1 (the
reference standard sequence) with
CYP4A22-WT (the most common allele)
revealed that for the four substrates
tested the WT-enzyme always had lower
activity.},
ISSN = {0014-5793},
DOI = {10.1002/1873-3468.13489},
url = {https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13489
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/1873-3468.13489},
year = {2019},
type = {Journal Article}
}
x
Functional characterization and mechanistic modeling of the human cytochrome P450 enzyme CYP4A22
The human cytochrome P450 (CYP) enzyme CYP4A22 is an orphan CYP with unknown function. Here, through functional expression in fission yeast, we show that CYP4A22 catalyzes fatty acid hydroxylation as well as aliphatic or aromatic hydroxylations of luciferin-based probe substrates. Mechanistic molecular modeling of CYP4A22 suggests that its ω-hydroxylation activity is hampered by a more spacious active site compared to CYP4B1. Substrate recognition via side-chains R96 and R233 is indicated by dynamic three-dimensional pharmacophores (dynophores) derived from molecular dynamics simulations. CYP4A22 activity is inhibited by three unspecific CYP inhibitors. A comparison of CYP4A22*1 (the reference standard sequence) with CYP4A22-WT (the most common allele) revealed that for the four substrates tested the WT-enzyme always had lower activity.
A. Nass, D. Schaller, and G. Wolber. Assessment of flexible shape complementarity: New opportunities to explain and induce selectivity in ligands of protein tyrosine phosphatase 1B, Mol Inf, 38(5):1800141, 2019.
Links:
[doi:10.1002/minf.201800141]
[show BibTeX]
[show abstract]
x
@article{RN268,
author = {Nass, A. and Schaller, D. and Wolber,
G.},
title = {Assessment of flexible shape
complementarity: New opportunities to
explain and induce selectivity in ligands
of protein tyrosine phosphatase 1B},
journal = {Molecular Informatics},
volume = {38},
number = {5},
pages = {1800141},
note = {Nass, Alexandra Schaller, David Wolber,
Gerhard eng Elsa-Neumann-Foundation
Germany Mol Inform. 2019 Feb 6. doi:
10.1002/minf.201800141.},
abstract = {For drug design projects it is essential
to rationally induce and explain
selectivity. In this context shape
complementarity as well as protein and
ligand flexibility represent important
factors. Currently available tools for the
analysis of protein-ligand interactions
focus mainly on electrostatic
complementarity and/or static structures.
Here we address the shortcomings of
available methods by presenting two new
tools: The first one can be used to assess
steric complementarity in flexible
protein-ligand complexes in order to
explain selectivity of known ligands. It
further allows to determine ligand atoms
with especially good or bad shape-fit
which can be of use in lead optimization
projects. The second tool was designed to
detect differences in protein flexibility
in similar proteins along with their
exploitation for virtual screening. Both
tools yield interesting results when
applied to data of protein tyrosine
phosphatase 1B (PTP1B): The case of PTP1B
has proven especially difficult in terms
of selectivity, due to a closely related
phosphatase connected to severe undesired
effects. With our tool for steric
complementarity assessment we were able to
explain previously undisclosed causes of
moderate selectivity of selected PTP1B
ligands. The second tool allowed us to
find differences of flexibility in the two
highly similar proteins and give
directions for exploitation in virtual
screening.},
keywords = {Computational chemistry Molecular
dynamics Molecular modeling Selectivity
Shape complementarity},
ISSN = {1868-1751 (Electronic) 1868-1743
(Linking)},
DOI = {10.1002/minf.201800141},
url = {https://www.ncbi.nlm.nih.gov/pubmed/30725529
https://onlinelibrary.wiley.com/doi/pdf/10.1002/minf.201800141},
year = {2019},
type = {Journal Article}
}
x
Assessment of flexible shape complementarity: New opportunities to explain and induce selectivity in ligands of protein tyrosine phosphatase 1B
For drug design projects it is essential to rationally induce and explain selectivity. In this context shape complementarity as well as protein and ligand flexibility represent important factors. Currently available tools for the analysis of protein-ligand interactions focus mainly on electrostatic complementarity and/or static structures. Here we address the shortcomings of available methods by presenting two new tools: The first one can be used to assess steric complementarity in flexible protein-ligand complexes in order to explain selectivity of known ligands. It further allows to determine ligand atoms with especially good or bad shape-fit which can be of use in lead optimization projects. The second tool was designed to detect differences in protein flexibility in similar proteins along with their exploitation for virtual screening. Both tools yield interesting results when applied to data of protein tyrosine phosphatase 1B (PTP1B): The case of PTP1B has proven especially difficult in terms of selectivity, due to a closely related phosphatase connected to severe undesired effects. With our tool for steric complementarity assessment we were able to explain previously undisclosed causes of moderate selectivity of selected PTP1B ligands. The second tool allowed us to find differences of flexibility in the two highly similar proteins and give directions for exploitation in virtual screening.
M. J. Ojeda-Montes, A. Casanova-Marti, A. Gimeno, S. Tomas-Hernandez, A. Cereto-Massague, G. Wolber, R. Beltran-Debon, C. Valls, M. Mulero, M. Pinent, G. Pujadas, and S. Garcia-Vallve. Mining large databases to find new leads with low similarity to known actives: application to find new DPP-IV inhibitors, Future Medicinal Chemistry, 11(12):1387-1401, 2019.
Links:
[doi:10.4155/fmc-2018-0597]
[show BibTeX]
[show abstract]
x
@article{RN272,
author = {Ojeda-Montes, M. J. and Casanova-Marti,
A. and Gimeno, A. and Tomas-Hernandez, S.
and Cereto-Massague, A. and Wolber, G. and
Beltran-Debon, R. and Valls, C. and
Mulero, M. and Pinent, M. and Pujadas, G.
and Garcia-Vallve, S.},
title = {Mining large databases to find new leads
with low similarity to known actives:
application to find new DPP-IV
inhibitors},
journal = {Future Medicinal Chemistry},
volume = {11},
number = {12},
pages = {1387-1401},
note = {Ojeda-Montes, Maria J Casanova-Marti,
Angela Gimeno, Aleix Tomas-Hernandez,
Sarah Cereto-Massague, Adria Wolber,
Gerhard Beltran-Debon, Raul Valls,
Cristina Mulero, Miquel Pinent, Montserrat
Pujadas, Gerard Garcia-Vallve, Santiago
eng England Future Med Chem. 2019
Jun;11(12):1387-1401. doi:
10.4155/fmc-2018-0597. Epub 2019 Jul 12.},
abstract = {Aim: Fragment-based drug design or
bioisosteric replacement is used to find
new actives with low (or no) similarity to
existing ones but requires the synthesis
of nonexisting compounds to prove their
predicted bioactivity. Protein-ligand
docking or pharmacophore screening are
alternatives but they can become
computationally expensive when applied to
very large databases such as ZINC.
Therefore, fast strategies are necessary
to find new leads in such databases.
Materials & methods: We designed a
computational strategy to find lead
molecules with very low (or no) similarity
to existing actives and applied it to
DPP-IV. Results: The bioactivity assays
confirm that this strategy finds new leads
for DPP-IV inhibitors. Conclusion: This
computational strategy reduces the time of
finding new lead molecules.},
keywords = {Cd26 dipeptidyl peptidase 4 diversifying
molecular scaffolds expanding chemical
space molecular fingerprints virtual
molecular libraries virtual screening},
ISSN = {1756-8927 (Electronic) 1756-8919
(Linking)},
DOI = {10.4155/fmc-2018-0597},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31298576},
year = {2019},
type = {Journal Article}
}
x
Mining large databases to find new leads with low similarity to known actives: application to find new DPP-IV inhibitors
Aim: Fragment-based drug design or bioisosteric replacement is used to find new actives with low (or no) similarity to existing ones but requires the synthesis of nonexisting compounds to prove their predicted bioactivity. Protein-ligand docking or pharmacophore screening are alternatives but they can become computationally expensive when applied to very large databases such as ZINC. Therefore, fast strategies are necessary to find new leads in such databases. Materials & methods: We designed a computational strategy to find lead molecules with very low (or no) similarity to existing actives and applied it to DPP-IV. Results: The bioactivity assays confirm that this strategy finds new leads for DPP-IV inhibitors. Conclusion: This computational strategy reduces the time of finding new lead molecules.
R. Ottanà, P. Paoli, G. Lori, I. Adornato, S. Previti, A. Naß, G. Wolber, and R. Maccari. Design and evaluation of non-carboxylate 5-arylidene-2-thioxo-4-imidazolidinones as novel non-competitive inhibitors of protein tyrosine phosphatase 1B, Bioorg Chem, 92:103211, 2019.
Links:
[doi:10.1016/j.bioorg.2019.103211]
[show BibTeX]
[show abstract]
x
@article{RN278,
author = {Ottanà, Rosaria and Paoli, Paolo and
Lori, Giulia and Adornato, Ilenia and
Previti, Santo and Naß, Alexandra and
Wolber, Gerhard and Maccari, Rosanna},
title = {Design and evaluation of non-carboxylate
5-arylidene-2-thioxo-4-imidazolidinones as
novel non-competitive inhibitors of
protein tyrosine phosphatase 1B},
journal = {Bioorganic Chemistry},
volume = {92},
pages = {103211},
abstract = {Protein tyrosine phosphatase 1B (PTP1B)
acts as a negative regulator of insulin
and leptin signalling and is crucially
involved in the development of type 2
diabetes mellitus, obesity, cancer and
neurodegenerative diseases. Pursuing our
efforts to identify PTP1B inhibitors
endowed with drug-like properties, we
designed and evaluated
3-aryl-5-arylidene-2-thioxo-4-imidazolidinones
(7) as a novel class of non-carboxylate
PTP1B inhibitors. In agreement with our
design, kinetic studies demonstrated that
selected compounds 7 act as reversible,
non-competitive inhibitors of the target
enzyme at low micromolar concentrations.
Accordingly, molecular docking experiments
suggested that these inhibitors can fit an
allosteric site of PTP1B that we
previously individuated. Moreover,
cellular assays demonstrated that compound
7e acts as a potent insulin-sensitizing
agent in human liver HepG2 cells. Taken
together, our results showed that these
non-competitive PTP1B inhibitors can be
considered promising lead compounds aimed
to enhance druggability of the target
enzyme and identify novel antidiabetic
drugs.},
keywords = {3-aryl-5-arylidene-2-thioxo-4-imidazolidinones
Protein tyrosine phosphatase 1B
Non-competitive inhibitors
Insulin-sensitizing agents Cellular
assays},
ISSN = {00452068},
DOI = {10.1016/j.bioorg.2019.103211},
url = {http://www.sciencedirect.com/science/article/pii/S0045206819307990
https://www.sciencedirect.com/science/article/abs/pii/S0045206819307990?via%3Dihub},
year = {2019},
type = {Journal Article}
}
x
Design and evaluation of non-carboxylate 5-arylidene-2-thioxo-4-imidazolidinones as novel non-competitive inhibitors of protein tyrosine phosphatase 1B
Protein tyrosine phosphatase 1B (PTP1B) acts as a negative regulator of insulin and leptin signalling and is crucially involved in the development of type 2 diabetes mellitus, obesity, cancer and neurodegenerative diseases. Pursuing our efforts to identify PTP1B inhibitors endowed with drug-like properties, we designed and evaluated 3-aryl-5-arylidene-2-thioxo-4-imidazolidinones (7) as a novel class of non-carboxylate PTP1B inhibitors. In agreement with our design, kinetic studies demonstrated that selected compounds 7 act as reversible, non-competitive inhibitors of the target enzyme at low micromolar concentrations. Accordingly, molecular docking experiments suggested that these inhibitors can fit an allosteric site of PTP1B that we previously individuated. Moreover, cellular assays demonstrated that compound 7e acts as a potent insulin-sensitizing agent in human liver HepG2 cells. Taken together, our results showed that these non-competitive PTP1B inhibitors can be considered promising lead compounds aimed to enhance druggability of the target enzyme and identify novel antidiabetic drugs.
D. Schaller, S. Hagenow, H. Stark, and G. Wolber. Ligand-guided homology modeling drives identification of novel histamine H3 receptor ligands, Plos One, 14(6):e0218820, 2019.
Links:
[doi:10.1371/journal.pone.0218820]
[show BibTeX]
[show abstract]
x
@article{RN274,
author = {Schaller, D. and Hagenow, S. and Stark,
H. and Wolber, G.},
title = {Ligand-guided homology modeling drives
identification of novel histamine H3
receptor ligands},
journal = {PLoS One},
volume = {14},
number = {6},
pages = {e0218820},
note = {Schaller, David Hagenow, Stefanie Stark,
Holger Wolber, Gerhard eng PLoS One. 2019
Jun 25;14(6):e0218820. doi:
10.1371/journal.pone.0218820. eCollection
2019.},
abstract = {In this study, we report a ligand-guided
homology modeling approach allowing the
analysis of relevant binding site residue
conformations and the identification of
two novel histamine H3 receptor ligands
with binding affinity in the nanomolar
range. The newly developed method is based
on exploiting an essential charge
interaction characteristic for aminergic
G-protein coupled receptors for ranking 3D
receptor models appropriate for the
discovery of novel compounds through
virtual screening.},
ISSN = {1932-6203 (Electronic) 1932-6203
(Linking)},
DOI = {10.1371/journal.pone.0218820},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31237914
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592549/pdf/pone.0218820.pdf},
year = {2019},
type = {Journal Article}
}
x
Ligand-guided homology modeling drives identification of novel histamine H3 receptor ligands
In this study, we report a ligand-guided homology modeling approach allowing the analysis of relevant binding site residue conformations and the identification of two novel histamine H3 receptor ligands with binding affinity in the nanomolar range. The newly developed method is based on exploiting an essential charge interaction characteristic for aminergic G-protein coupled receptors for ranking 3D receptor models appropriate for the discovery of novel compounds through virtual screening.
D. Schaller, S. Pach, and G. Wolber. PyRod: Tracing water molecules in molecular dynamics simulations, J Chem Inf Model, 59(6):2818-2829, 2019.
Links:
[doi:10.1021/acs.jcim.9b00281]
[show BibTeX]
[show abstract]
x
@article{RN270,
author = {Schaller, D. and Pach, S. and Wolber,
G.},
title = {PyRod: Tracing water molecules in
molecular dynamics simulations},
journal = {Journal of Chemical Information and
Modeling},
volume = {59},
number = {6},
pages = {2818-2829},
note = {Schaller, David Pach, Szymon Wolber,
Gerhard eng J Chem Inf Model. 2019 Jun
24;59(6):2818-2829. doi:
10.1021/acs.jcim.9b00281. Epub 2019 May
30.},
abstract = {Ligands entering a protein binding pocket
essentially compete with water molecules
for binding to the protein. Hence, the
location and thermodynamic properties of
water molecules in protein structures have
gained increased attention in the drug
design community. Including corresponding
data into 3D pharmacophore modeling is
essential for efficient high throughput
virtual screening. Here, we present PyRod,
a free and open-source Python software
that allows for visualization of
pharmacophoric binding pocket
characteristics, identification of hot
spots for ligand binding, and subsequent
generation of pharmacophore features for
virtual screening. The implemented
routines analyze the protein environment
of water molecules in molecular dynamics
(MD) simulations and can differentiate
between hydrogen bonded waters as well as
waters in a protein environment of
hydrophobic, charged, or aromatic atom
groups. The gathered information is
further processed to generate dynamic
molecular interaction fields (dMIFs) for
visualization and pharmacophoric features
for virtual screening. The described
software was applied to 5 therapeutically
relevant drug targets, and generated
pharmacophores were evaluated using DUD-E
benchmarking sets. The best performing
pharmacophore was found for the HIV1
protease with an early enrichment factor
of 54.6. PyRod adds a new perspective to
structure-based screening campaigns by
providing easy-to-interpret dMIFs and
purely protein-based pharmacophores that
are solely based on tracing water
molecules in MD simulations. Since
structural information about
cocrystallized ligands is not needed,
screening campaigns can be followed, for
which less or no ligand information is
available. PyRod is freely available at
https://github.com/schallerdavid/pyrod .},
keywords = {software},
ISSN = {1549-960X (Electronic) 1549-9596
(Linking)},
DOI = {10.1021/acs.jcim.9b00281},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31117512},
year = {2019},
type = {Journal Article}
}
x
PyRod: Tracing water molecules in molecular dynamics simulations
Ligands entering a protein binding pocket essentially compete with water molecules for binding to the protein. Hence, the location and thermodynamic properties of water molecules in protein structures have gained increased attention in the drug design community. Including corresponding data into 3D pharmacophore modeling is essential for efficient high throughput virtual screening. Here, we present PyRod, a free and open-source Python software that allows for visualization of pharmacophoric binding pocket characteristics, identification of hot spots for ligand binding, and subsequent generation of pharmacophore features for virtual screening. The implemented routines analyze the protein environment of water molecules in molecular dynamics (MD) simulations and can differentiate between hydrogen bonded waters as well as waters in a protein environment of hydrophobic, charged, or aromatic atom groups. The gathered information is further processed to generate dynamic molecular interaction fields (dMIFs) for visualization and pharmacophoric features for virtual screening. The described software was applied to 5 therapeutically relevant drug targets, and generated pharmacophores were evaluated using DUD-E benchmarking sets. The best performing pharmacophore was found for the HIV1 protease with an early enrichment factor of 54.6. PyRod adds a new perspective to structure-based screening campaigns by providing easy-to-interpret dMIFs and purely protein-based pharmacophores that are solely based on tracing water molecules in MD simulations. Since structural information about cocrystallized ligands is not needed, screening campaigns can be followed, for which less or no ligand information is available. PyRod is freely available at https://github.com/schallerdavid/pyrod .
S. Schramm, L. Agnetta, M. Bermudez, H. Gerwe, M. Irmen, J. Holze, T. Littmann, G. Wolber, C. Trankle, and M. Decker. Novel BQCA- and TBPB-derived M1 receptor hybrid ligands: Orthosteric carbachol differentially regulates partial agonism, ChemMedChem, 14(14):1349-1358, 2019.
Links:
[doi:10.1002/cmdc.201900283]
[show BibTeX]
[show abstract]
x
@article{RN269,
author = {Schramm, S. and Agnetta, L. and Bermudez,
M. and Gerwe, H. and Irmen, M. and Holze,
J. and Littmann, T. and Wolber, G. and
Trankle, C. and Decker, M.},
title = {Novel BQCA- and TBPB-derived M1 receptor
hybrid ligands: Orthosteric carbachol
differentially regulates partial agonism},
journal = {ChemMedChem},
volume = {14},
number = {14},
pages = {1349-1358},
note = {Schramm, Simon Agnetta, Luca Bermudez,
Marcel Gerwe, Hubert Irmen, Matthias
Holze, Janine Littmann, Timo Wolber,
Gerhard Trankle, Christian Decker, Michael
eng International Doctoral program
"Receptor Dynamics"/Elitenetzwerk Bayern
Germany ChemMedChem. 2019 Jul
17;14(14):1349-1358. doi:
10.1002/cmdc.201900283. Epub 2019 Jul 3.},
abstract = {Recently, investigations of the complex
mechanisms of allostery have led to a
deeper understanding of G protein-coupled
receptor (GPCR) activation and signaling
processes. In this context, muscarinic
acetylcholine receptors (mAChRs) are
highly relevant due to their exemplary
role in the study of allosteric
modulation. In this work, we compare and
discuss two sets of putatively dualsteric
ligands, which were designed to connect
carbachol to different types of allosteric
ligands. We chose derivatives of TBPB
[1-(1'-(2-tolyl)-1,4'-bipiperidin-4-yl)-1H-benzo[d]imidazol-2(3H)-one]
as M1 -selective putative bitopic ligands,
and derivatives of benzyl quinolone
carboxylic acid (BQCA) as an M1 positive
allosteric modulator, varying the distance
between the allosteric and orthosteric
building blocks. Luciferase protein
complementation assays demonstrated that
linker length must be carefully chosen to
yield either agonist or antagonist
behavior. These findings may help to
design biased signaling and/or different
extents of efficacy.},
keywords = {GPCRs allostery dualsteric ligands
muscarinic receptors partial agonists},
ISSN = {1860-7187 (Electronic) 1860-7179
(Linking)},
DOI = {10.1002/cmdc.201900283},
url = {https://www.ncbi.nlm.nih.gov/pubmed/31166078
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cmdc.201900283},
year = {2019},
type = {Journal Article}
}
x
Novel BQCA- and TBPB-derived M1 receptor hybrid ligands: Orthosteric carbachol differentially regulates partial agonism
Recently, investigations of the complex mechanisms of allostery have led to a deeper understanding of G protein-coupled receptor (GPCR) activation and signaling processes. In this context, muscarinic acetylcholine receptors (mAChRs) are highly relevant due to their exemplary role in the study of allosteric modulation. In this work, we compare and discuss two sets of putatively dualsteric ligands, which were designed to connect carbachol to different types of allosteric ligands. We chose derivatives of TBPB [1-(1'-(2-tolyl)-1,4'-bipiperidin-4-yl)-1H-benzo[d]imidazol-2(3H)-one] as M1 -selective putative bitopic ligands, and derivatives of benzyl quinolone carboxylic acid (BQCA) as an M1 positive allosteric modulator, varying the distance between the allosteric and orthosteric building blocks. Luciferase protein complementation assays demonstrated that linker length must be carefully chosen to yield either agonist or antagonist behavior. These findings may help to design biased signaling and/or different extents of efficacy.
D. Sribar, M. Grabowski, M. S. Murgueitio, M. Bermudez, G. Weindl, and G. Wolber. Identification and characterization of a novel chemotype for human TLR8 inhibitors, Eur J Med Chem, 179:744-752, 2019.
Links:
[doi:10.1016/j.ejmech.2019.06.084]
[show BibTeX]
[show abstract]
x
@article{RN273,
author = {Sribar, D. and Grabowski, M. and
Murgueitio, M. S. and Bermudez, M. and
Weindl, G. and Wolber, G.},
title = {Identification and characterization of a
novel chemotype for human TLR8
inhibitors},
journal = {European Journal of Medicinal Chemistry},
volume = {179},
pages = {744-752},
note = {Sribar, Dora Grabowski, Maria Murgueitio,
Manuela S Bermudez, Marcel Weindl, Gunther
Wolber, Gerhard eng France Eur J Med Chem.
2019 Jun 29;179:744-752. doi:
10.1016/j.ejmech.2019.06.084.},
abstract = {The endosomal Toll-like receptor 8 (TLR8)
recognizes single-stranded RNA and
initiates early inflammatory responses.
Despite the importance of endosomal TLRs
for human host defense against microbial
pathogens, extensive activation may
contribute to autoimmune and inflammatory
diseases. In contrast to the recent
progress made in the development of
modulators of plasma membrane-bound TLRs,
little is known about endosomal TLR
modulation and very few TLR8 inhibitors
have been reported. In this study, we
discovered and validated novel
small-molecule TLR8 inhibitors. Fourteen
potential TLR8 modulators were
experimentally validated in HEK293T cells
stably overexpressing human TLR8 and THP-1
macrophages. Five compounds inhibited
TLR8-mediated signaling, representing a
hit rate of 36%. The three most potent
compounds neither cause cellular toxicity
nor inhibition of TLR signaling induced by
other receptor subtypes. Conclusively, we
experimentally confirm novel and
selective, pyrimidine-based TLR8
inhibitors with low cytotoxicity that are
relevant candidates for lead optimization
and further mechanistic studies.},
keywords = {Inflammation Inhibitors Pyrimidine
scaffold Toll-like receptor 8 Virtual
screening},
ISSN = {1768-3254 (Electronic) 0223-5234
(Linking)},
DOI = {10.1016/j.ejmech.2019.06.084},
year = {2019},
type = {Journal Article}
}
x
Identification and characterization of a novel chemotype for human TLR8 inhibitors
The endosomal Toll-like receptor 8 (TLR8) recognizes single-stranded RNA and initiates early inflammatory responses. Despite the importance of endosomal TLRs for human host defense against microbial pathogens, extensive activation may contribute to autoimmune and inflammatory diseases. In contrast to the recent progress made in the development of modulators of plasma membrane-bound TLRs, little is known about endosomal TLR modulation and very few TLR8 inhibitors have been reported. In this study, we discovered and validated novel small-molecule TLR8 inhibitors. Fourteen potential TLR8 modulators were experimentally validated in HEK293T cells stably overexpressing human TLR8 and THP-1 macrophages. Five compounds inhibited TLR8-mediated signaling, representing a hit rate of 36%. The three most potent compounds neither cause cellular toxicity nor inhibition of TLR signaling induced by other receptor subtypes. Conclusively, we experimentally confirm novel and selective, pyrimidine-based TLR8 inhibitors with low cytotoxicity that are relevant candidates for lead optimization and further mechanistic studies.
A. Stoll, S. Loke, J. F. Joseph, D. Machalz, X. de la Torre, F. Botrè, G. Wolber, M. Bureik, and M. K. Parr. Fine-mapping of the substrate specificity of human steroid 21-hydroxylase (CYP21A2), J Steroid Biochem, 194:105446, 2019.
Links:
[doi:10.1016/j.jsbmb.2019.105446]
[show BibTeX]
[show abstract]
x
@article{RN277,
author = {Stoll, Anna and Loke, Steffen and Joseph,
Jan Felix and Machalz, David and de la
Torre, Xavier and Botrè, Francesco and
Wolber, Gerhard and Bureik, Matthias and
Parr, Maria Kristina},
title = {Fine-mapping of the substrate specificity
of human steroid 21-hydroxylase
(CYP21A2)},
journal = {Journal of Steroid Biochemistry and
Molecular Biology},
volume = {194},
pages = {105446},
abstract = {Cytochrome P450 enzymes (CYPs) are
capable of catalyzing regio- and
stereo-specific oxy functionalization
reactions which otherwise are major
challenges in organic chemistry. In order
to make the best possible use of these
biocatalysts it is imperative to
understand their specificities. Human
CYP21A2 (steroid 21-hydroxylase) acts on
the side-chain attached to C-17 in ring D
of a steroid substrate, but the
configuration of ring A also plays a
prominent role in substrate cognition.
Here, we comprehensively investigated this
relationship using sixteen
17,17-dimethyl-18-nor-13-ene steroids with
different arrangements of hydroxy-, oxo-,
fluoro- and chloro-groups and in the
presence or absence of double bonds (Δ1
and/or Δ4) and heteroatoms in ring A. The
results show that presence of a 3-oxo
group is a strict requirement for a
CYP21A2 substrate, while the other
configurations tested were all tolerated.
This was also confirmed by control
experiments using endogenous steroids.
While progesterone and
17-hydroxyprogesterone were hydroxylated
at C-21, (17-hydroxy-) pregnenolone did
not react. Molecular docking experiments
indicate that the interaction of the
carbonyl group at C-3 to the side-chain
Arg234 of the enzyme is indispensable.},
keywords = {Cytochrome P450 Fission yeast Gas
chromatography mass spectrometry (GCMS)
Oral turinabol long-term metabolite
Molecular modeling Steroid hydroxylation},
ISSN = {0960-0760},
DOI = {10.1016/j.jsbmb.2019.105446},
url = {http://www.sciencedirect.com/science/article/pii/S0960076019304169
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year = {2019},
type = {Journal Article}
}
x
Fine-mapping of the substrate specificity of human steroid 21-hydroxylase (CYP21A2)
Cytochrome P450 enzymes (CYPs) are capable of catalyzing regio- and stereo-specific oxy functionalization reactions which otherwise are major challenges in organic chemistry. In order to make the best possible use of these biocatalysts it is imperative to understand their specificities. Human CYP21A2 (steroid 21-hydroxylase) acts on the side-chain attached to C-17 in ring D of a steroid substrate, but the configuration of ring A also plays a prominent role in substrate cognition. Here, we comprehensively investigated this relationship using sixteen 17,17-dimethyl-18-nor-13-ene steroids with different arrangements of hydroxy-, oxo-, fluoro- and chloro-groups and in the presence or absence of double bonds (Δ1 and/or Δ4) and heteroatoms in ring A. The results show that presence of a 3-oxo group is a strict requirement for a CYP21A2 substrate, while the other configurations tested were all tolerated. This was also confirmed by control experiments using endogenous steroids. While progesterone and 17-hydroxyprogesterone were hydroxylated at C-21, (17-hydroxy-) pregnenolone did not react. Molecular docking experiments indicate that the interaction of the carbonyl group at C-3 to the side-chain Arg234 of the enzyme is indispensable.
N. K. Wenke, J. Kreye, E. Andrzejak, A. van Casteren, J. Leubner, M. S. Murgueitio, S. M. Reincke, C. Secker, L. Schmidl, C. Geis, F. Ackermann, M. Nikolaus, C. C. Garner, H. Wardemann, G. Wolber, and H. Pruess. NMDA receptor dysfunction via unmutated human antibodies against the NR1 subunit, Ann Neurol, 85(5):771-776, 2019.
Links:
[doi:10.1002/ana.25460]
[show BibTeX]
[show abstract]
x
@article{RN266,
author = {Wenke, Nina Kerstin and Kreye, Jakob and
Andrzejak, Ewa and van Casteren, Adriana
and Leubner, Jonas and Murgueitio, Manuela
S. and Reincke, S. Momsen and Secker,
Christopher and Schmidl, Lars and Geis,
Christian and Ackermann, Frauke and
Nikolaus, Marc and Garner, Craig C. and
Wardemann, Hedda and Wolber, Gerhard and
Pruess, Harald},
title = {NMDA receptor dysfunction via unmutated
human antibodies against the NR1 subunit},
journal = {Annals of neurology},
volume = {85},
number = {5},
pages = {771-776},
abstract = {Anti‐N‐methyl‐D‐aspartate‐receptor
(NMDAR) encephalitis is the most common
autoimmune encephalitis related to
autoantibody‐mediated synaptic
dysfunction. Cerebrospinal fluid‐derived
human monoclonal NR1 autoantibodies showed
low numbers of somatic hypermutations or
were unmutated. These unexpected
germline‐configured antibodies showed
weaker binding to the NMDAR than matured
antibodies from the same patient. In
primary hippocampal neurons, germline NR1
autoantibodies strongly and specifically
reduced total and synaptic NMDAR currents
in a dose‐ and time‐dependent manner.
The findings suggest that functional NMDAR
antibodies are part of the human naïve B
cell repertoire. Given their effects on
synaptic function, they might contribute
to a broad spectrum of neuropsychiatric
symptoms.},
ISSN = {0364-5134},
DOI = {10.1002/ana.25460},
url = {http://europepmc.org/abstract/MED/30843274
https://doi.org/10.1002/ana.25460
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593665/pdf/ANA-85-771.pdf},
year = {2019},
type = {Journal Article}
}
x
NMDA receptor dysfunction via unmutated human antibodies against the NR1 subunit
Anti‐N‐methyl‐D‐aspartate‐receptor (NMDAR) encephalitis is the most common autoimmune encephalitis related to autoantibody‐mediated synaptic dysfunction. Cerebrospinal fluid‐derived human monoclonal NR1 autoantibodies showed low numbers of somatic hypermutations or were unmutated. These unexpected germline‐configured antibodies showed weaker binding to the NMDAR than matured antibodies from the same patient. In primary hippocampal neurons, germline NR1 autoantibodies strongly and specifically reduced total and synaptic NMDAR currents in a dose‐ and time‐dependent manner. The findings suggest that functional NMDAR antibodies are part of the human naïve B cell repertoire. Given their effects on synaptic function, they might contribute to a broad spectrum of neuropsychiatric symptoms.
O. Zierau, A. Kolodziejczyk, G. Vollmer, D. Machalz, G. Wolber, D. Thieme, and A. M. Keiler. Comparison of the three SARMs RAD-140, GLPG0492 and GSK-2881078 in two different in vitro bioassays, and in an in silico androgen receptor binding assay, J Steroid Biochem, 189:81-86, 2019.
Links:
[doi:10.1016/j.jsbmb.2019.02.014]
[show BibTeX]
[show abstract]
x
@article{RN265,
author = {Zierau, Oliver and Kolodziejczyk, Annika
and Vollmer, Günter and Machalz, David
and Wolber, Gerhard and Thieme, Detlef and
Keiler, Annekathrin Martina},
title = {Comparison of the three SARMs RAD-140,
GLPG0492 and GSK-2881078 in two different
in vitro bioassays, and in an in silico
androgen receptor binding assay},
journal = {Journal of Steroid Biochemistry and
Molecular Biology},
volume = {189},
pages = {81-86},
abstract = {Selective androgen receptor modulators
comprise compounds that bind as ligands to
the androgen receptor and possess
tissue-selective activities. Ideally, they
show agonistic properties in anabolic
target tissues, while inducing
antagonistic or only weak agonistic
effects in reproductive organs. Due to
their myoanabolic effects, selective
androgen receptor modulators are included
in the list of prohibited substances and
methods of the World Anti-Doping Agency.
In the current investigation, the
androgenic potential of RAD-140,
GSK-2881078 and GLPG0492 was comparably
investigated in two different in vitro
bioassays. In the yeast androgen screen,
the androgenic effects were lower than in
the reporter gene assay in prostate
carcinoma cells (e.g. for GSK-2881078, the
EC50 values were 4.44 × 10−6M in
the yeast screen and 3.99 × 10-9M in
the prostate cells respectively). For
future investigations, it is of importance
whether the yeast androgen screen, which
has been proven to detect androgenic
compounds in urine, can detect an abuse of
the selective androgen receptor
modulators. Molecular modeling of the
binding to the androgen receptor ligand
binding domain suggests slight differences
in the binding modes of RAD-140,
GSK-2881078 and GLPG0492. In conclusion,
androgenic activity of the three
non-steroidal compounds in the two
different in vitro test systems confirmed
the results of the in silico modeling of
the androgen receptor binding.},
keywords = {Selective androgen receptor modulators
Yeast androgen screen PC3(AR)cells
Molecular modeling},
ISSN = {0960-0760},
DOI = {10.1016/j.jsbmb.2019.02.014},
url = {http://www.sciencedirect.com/science/article/pii/S0960076018306885
https://pdf.sciencedirectassets.com/271264/1-s2.0-S0960076019X00047/1-s2.0-S0960076018306885/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJr%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIBmGoQIBcjjZ5EaOK3PJ%2FGurZx%2F3iAg7AxgzJBGlS1pLAiEAqq3PoXrNmhK0J8VvhGKQxD8CDm2MMxpACdgEvvCDCF0q2gMIQhACGgwwNTkwMDM1NDY4NjUiDB%2FjPXYQeqnKPMIAZSq3Axc0m%2BRD9Wt3prnTKye3Cqvo0AREbqzZ946GaterseGXxpe%2BUb3tyOJVBoXAsvayOEvYdT75FjY53skFYyeai6OYOXjZRWTEelsGyFCQf8cT3DEzx4Q%2FSuFEL5blDoqhss6bszDRC5%2FuVVpSzUmF7%2BFyAgGxxPdatNCbBmiutHGyRMn4PicJgX1DP70wJovm2MIIfOQjtVBAoW1FPcQfo4A0fJI5ShSReUYAnaXr04R8cTE%2BTTnwOVhgNPw19doX3%2FZOAC3wHvx2xdw427PkU7STXll%2FivSFUP2UPMpj5wiro1CxQicswmv0RAWfVQV%2BoN9oIuCE4eWm%2FoT86KudK0SbLCkH8hiO85Gkxien8zQf3J%2BA2aXelkRNwjG55dMxfLKxuLd5mhDnokYj7cOpwsS9sVe2WOpNw0L83GU%2FpuvVJI9vZFARLk225K%2FQ4b1pFcwGOKOAZBiAQZJ9D7JWTWR0gA8Y9KTN%2FrSCDcM7e3OCrDWcifFbCTor1FSCUFanT4fycawD7p8nphvKBxLQcZTmUo0CLG0aAL6hXvuI45m7qu0ijPUXIyCJTvyurXi9RPRuVGihaxkw98KO6wU6tAGChddaHRM2ELzStD4I3%2BltvO8qU5OvXkFpFPXsJT4YQCQafydsDiutI4rsZ8MyFKk2VmqAYvFy3yg9tm4P88k25cm8YxrYD9FZBt34b46IAXloCTW%2BjWdZAQpgxFCuBBURFwPqw%2Bz5EL4gHYjp%2BQW%2FHnmtR8SfofKETRV74Znvs9OncFyjtB09l8I8dyXKRPvZSo%2BtyGyKRjRStMbqfOcty%2BjSd%2FWSyeoJFlE6ZwZclpM4vEI%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090959Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYQ4T4FY7Z%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=eeebe89dc7b803edab1802d7f7937d0988afcf62b96a832e6f62ad813ee4b3c7&hash=80147c3643733f04f0be90d02fa9badc86eacb637119a9c2ccefcd8a0217a49c&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0960076018306885&tid=spdf-742e1312-8b0a-4fb1-a7fe-20eb2f8b370f&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2019},
type = {Journal Article}
}
x
Comparison of the three SARMs RAD-140, GLPG0492 and GSK-2881078 in two different in vitro bioassays, and in an in silico androgen receptor binding assay
Selective androgen receptor modulators comprise compounds that bind as ligands to the androgen receptor and possess tissue-selective activities. Ideally, they show agonistic properties in anabolic target tissues, while inducing antagonistic or only weak agonistic effects in reproductive organs. Due to their myoanabolic effects, selective androgen receptor modulators are included in the list of prohibited substances and methods of the World Anti-Doping Agency. In the current investigation, the androgenic potential of RAD-140, GSK-2881078 and GLPG0492 was comparably investigated in two different in vitro bioassays. In the yeast androgen screen, the androgenic effects were lower than in the reporter gene assay in prostate carcinoma cells (e.g. for GSK-2881078, the EC50 values were 4.44 × 10−6M in the yeast screen and 3.99 × 10-9M in the prostate cells respectively). For future investigations, it is of importance whether the yeast androgen screen, which has been proven to detect androgenic compounds in urine, can detect an abuse of the selective androgen receptor modulators. Molecular modeling of the binding to the androgen receptor ligand binding domain suggests slight differences in the binding modes of RAD-140, GSK-2881078 and GLPG0492. In conclusion, androgenic activity of the three non-steroidal compounds in the two different in vitro test systems confirmed the results of the in silico modeling of the androgen receptor binding.
M. Grabowski, M. S. Murgueitio, M. Bermudez, J. Rademann, G. Wolber, and G. Weindl. Identification of a pyrogallol derivative as a potent and selective human TLR2 antagonist by structure-based virtual screening, Biochem Pharmacol, 154:148-160, 2018.
Links:
[doi:10.1016/j.bcp.2018.04.018]
[show BibTeX]
[show abstract]
x
@article{RN260,
author = {Grabowski, Maria and Murgueitio, Manuela
S. and Bermudez, Marcel and Rademann,
Jörg and Wolber, Gerhard and Weindl,
Günther},
title = {Identification of a pyrogallol derivative
as a potent and selective human TLR2
antagonist by structure-based virtual
screening},
journal = {Biochemical Pharmacology},
volume = {154},
pages = {148-160},
abstract = {Toll-like receptor 2 (TLR2) induces early
inflammatory responses to pathogen and
damage-associated molecular patterns
trough heterodimerization with either TLR1
or TLR6. Since overstimulation of TLR2
signaling is linked to several
inflammatory and metabolic diseases, TLR2
antagonists may provide therapeutic
benefits for the control of inflammatory
conditions. We present virtual screening
for the identification of novel TLR2
modulators, which combines analyses of
known ligand sets with structure-based
approaches. The 13 identified compounds
were pharmacologically characterized in
HEK293-hTLR2 cells, THP-1 macrophages and
peripheral blood mononuclear cells for
their ability to inhibit TLR2-mediated
responses. Four out of 13 selected
compounds show concentration-dependent
activity, representing a hit rate of 31%.
The most active compound is the pyrogallol
derivative MMG-11 that inhibits both
TLR2/1 and TLR2/6 signaling and shows a
higher potency than the previously
discovered CU-CPT22. Concentration ratio
analysis identified both compounds as
competitive antagonists of Pam3CSK4- and
Pam2CSK4-induced responses. Schild plot
analysis yielded apparent pA2 values of
5.73 and 6.15 (TLR2/1), and 5.80 and 6.65
(TLR2/6) for CU-CPT22 and MMG-11,
respectively. MMG-11 neither shows
cellular toxicity nor interference with
signaling induced by other TLR agonists,
IL-1β or TNF. Taken together, we
demonstrate that MMG-11 is a potent and
selective TLR2 antagonist with low
cytotoxicity rendering it a promising
pharmacological tool for the investigation
of TLR signaling and a suitable lead
structure for further chemical
optimization.},
keywords = {Drug discovery Toll-like receptors TLR2
Virtual screening Competitive antagonist
tlr},
ISSN = {0006-2952},
DOI = {10.1016/j.bcp.2018.04.018},
year = {2018},
type = {Journal Article}
}
x
Identification of a pyrogallol derivative as a potent and selective human TLR2 antagonist by structure-based virtual screening
Toll-like receptor 2 (TLR2) induces early inflammatory responses to pathogen and damage-associated molecular patterns trough heterodimerization with either TLR1 or TLR6. Since overstimulation of TLR2 signaling is linked to several inflammatory and metabolic diseases, TLR2 antagonists may provide therapeutic benefits for the control of inflammatory conditions. We present virtual screening for the identification of novel TLR2 modulators, which combines analyses of known ligand sets with structure-based approaches. The 13 identified compounds were pharmacologically characterized in HEK293-hTLR2 cells, THP-1 macrophages and peripheral blood mononuclear cells for their ability to inhibit TLR2-mediated responses. Four out of 13 selected compounds show concentration-dependent activity, representing a hit rate of 31%. The most active compound is the pyrogallol derivative MMG-11 that inhibits both TLR2/1 and TLR2/6 signaling and shows a higher potency than the previously discovered CU-CPT22. Concentration ratio analysis identified both compounds as competitive antagonists of Pam3CSK4- and Pam2CSK4-induced responses. Schild plot analysis yielded apparent pA2 values of 5.73 and 6.15 (TLR2/1), and 5.80 and 6.65 (TLR2/6) for CU-CPT22 and MMG-11, respectively. MMG-11 neither shows cellular toxicity nor interference with signaling induced by other TLR agonists, IL-1β or TNF. Taken together, we demonstrate that MMG-11 is a potent and selective TLR2 antagonist with low cytotoxicity rendering it a promising pharmacological tool for the investigation of TLR signaling and a suitable lead structure for further chemical optimization.
A. M. Keiler, O. Zierau, S. Wolf, P. Diel, W. Schänzer, G. Vollmer, D. Machalz, G. Wolber, and M. K. Parr. Androgen- and estrogen-receptor mediated activities of 4-hydroxytestosterone, 4-hydroxyandrostenedione and their human metabolites in yeast based assays, Toxicol Lett, 292:39-45, 2018.
Links:
[doi:10.1016/j.toxlet.2018.04.026]
[show BibTeX]
[show abstract]
x
@article{RN256,
author = {Keiler, Annekathrin Martina and Zierau,
Oliver and Wolf, Sylvi and Diel, Patrick
and Schänzer, Wilhelm and Vollmer,
Günter and Machalz, David and Wolber,
Gerhard and Parr, Maria Kristina},
title = {Androgen- and estrogen-receptor mediated
activities of 4-hydroxytestosterone,
4-hydroxyandrostenedione and their human
metabolites in yeast based assays},
journal = {Toxicology Letters},
volume = {292},
pages = {39-45},
abstract = {4-Hydroxyandrost-4-ene-3,17-dione, also
named formestane, is an irreversible
aromatase inhibitor and therapeutically
used as anti-breast cancer medication in
post-menopausal women. Currently, no
therapeutical indication led to approval
of its 17-hydroxylated analog
4-hydroxytestosterone, an anabolic
steroid. However, it is currently
investigated in a clinical trial for
breast cancer. In context with sports
doping, aromatase inhibitors are
administered to reduce estrogenic side
effects of misused anabolic substances or
their metabolites. Therefore, both
substances are prohibited in sports by the
World Anti-Doping Agency (WADA). Analysis
of urinary phase I and phase II
metabolites showed similar results for
both compounds. In the current
investigation,
4-hydroxyandrost-4-ene-3,17-dione,
4-hydroxytestosterone and seven of their
described urinary metabolites as well as
2α-hydroxyandrostenedione were tested in
the yeast androgen screen and the yeast
estrogen screen. Androgenic effects were
observed for all tested substances, except
for one, which showed anti-androgenic
properties. With regard to the yeast
estrogen screen, estrogenic effects were
observed for only two metabolites at
rather high concentrations, while six out
of the ten substances tested showed
anti-estrogenic properties. In terms of
the strong androgenic effect observed for
4-hydroxytestosterone (10−8 M),
4-hydroxyandrost-4-ene-3,17-dione
(10−8 M) and two more urinary
metabolites, the yeast androgen assay may
also be used to trace abuse in urine
samples.},
keywords = {Formestane 4-Hydroxytestosterone Doping
Molecular modeling},
ISSN = {0378-4274},
DOI = {10.1016/j.toxlet.2018.04.026},
url = {http://www.sciencedirect.com/science/article/pii/S0378427418301644
https://www.sciencedirect.com/science/article/pii/S0378427418301644?via%3Dihub},
year = {2018},
type = {Journal Article}
}
x
Androgen- and estrogen-receptor mediated activities of 4-hydroxytestosterone, 4-hydroxyandrostenedione and their human metabolites in yeast based assays
4-Hydroxyandrost-4-ene-3,17-dione, also named formestane, is an irreversible aromatase inhibitor and therapeutically used as anti-breast cancer medication in post-menopausal women. Currently, no therapeutical indication led to approval of its 17-hydroxylated analog 4-hydroxytestosterone, an anabolic steroid. However, it is currently investigated in a clinical trial for breast cancer. In context with sports doping, aromatase inhibitors are administered to reduce estrogenic side effects of misused anabolic substances or their metabolites. Therefore, both substances are prohibited in sports by the World Anti-Doping Agency (WADA). Analysis of urinary phase I and phase II metabolites showed similar results for both compounds. In the current investigation, 4-hydroxyandrost-4-ene-3,17-dione, 4-hydroxytestosterone and seven of their described urinary metabolites as well as 2α-hydroxyandrostenedione were tested in the yeast androgen screen and the yeast estrogen screen. Androgenic effects were observed for all tested substances, except for one, which showed anti-androgenic properties. With regard to the yeast estrogen screen, estrogenic effects were observed for only two metabolites at rather high concentrations, while six out of the ten substances tested showed anti-estrogenic properties. In terms of the strong androgenic effect observed for 4-hydroxytestosterone (10−8 M), 4-hydroxyandrost-4-ene-3,17-dione (10−8 M) and two more urinary metabolites, the yeast androgen assay may also be used to trace abuse in urine samples.
J. Liu, L. Chen, J. F. Joseph, A. Naß, A. Stoll, X. de la Torre, F. Botrè, G. Wolber, M. K. Parr, and M. Bureik. Combined chemical and biotechnological production of 20βOH-NorDHCMT, a long-term metabolite of Oral-Turinabol (DHCMT), J Inorg Biochem, 183:165-171, 2018.
Links:
[doi:10.1016/j.jinorgbio.2018.02.020]
[show BibTeX]
[show abstract]
x
@article{RN257,
author = {Liu, Jiaxin and Chen, Lei and Joseph, Jan
Felix and Naß, Alexandra and Stoll, Anna
and de la Torre, Xavier and Botrè,
Francesco and Wolber, Gerhard and Parr,
Maria Kristina and Bureik, Matthias},
title = {Combined chemical and biotechnological
production of 20βOH-NorDHCMT, a long-term
metabolite of Oral-Turinabol (DHCMT)},
journal = {Journal of Inorganic Biochemistry},
volume = {183},
pages = {165-171},
abstract = {Anabolic androgenic steroids (AAS) are
misused very frequently in sport
competitions as performance enhancing
agents. One of the doping compounds that
has been detected with increased frequency
in the last few years is
dehydrochloromethyltestosterone (DHCMT,
4-chloro-17β-hydroxy-17α-methylandrosta-1,4-dien-3-one;
brand name Oral Turinabol). The long-term
DHCMT metabolite 20βOH-NorDHCMT
(4-chloro-17β-hydroxymethyl-17α-methyl-18-norandrosta-1,4,13-trien-3-one)
was reported earlier to be detectable in
urine samples for more than 22 days
after DHCMT administration; however,
purified reference material was not
available so far. In this study we
demonstrate a successful combination of
Wagner-Meerwein rearrangement of DHCMT to
NorDHCMT
(4-chloro-17,17-dimethyl-18-norandrosta-1,4,13-trien-3-one)
and subsequent whole-cell
biotransformation with a recombinant
fission yeast strain expressing the human
cytochrome P450 enzyme (CYP or P450)
CYP21A2 for the synthesis of mg amounts of
this metabolite. It was then used as
reference for the analysis of a post
administration urine of DHCMT. The
availability of this reference compound
will provide an incontestable proof for
DHCMT abuse.},
keywords = {Anabolic androgenic steroids Doping
Fission yeast Whole-cell
biotransformation},
ISSN = {0162-0134},
DOI = {10.1016/j.jinorgbio.2018.02.020},
url = {http://www.sciencedirect.com/science/article/pii/S0162013417308516
https://pdf.sciencedirectassets.com/271941/1-s2.0-S0162013418X0004X/1-s2.0-S0162013417308516/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJGMEQCIBSuXR284U5c9bmAJSN5zVw47n5M5VTNwrBmGQ8hLXVcAiAWIgoCDAYq%2BrLMnoeOj0gcwMsJ%2BK2%2F2vx37jlwfG9V%2FiraAwhBEAIaDDA1OTAwMzU0Njg2NSIMjEBFMn9jIE9t1XN2KrcDLe7GRG%2FXcw3WEgKV9YXbSGYcHYB2z7r%2B9eRerOT0zpE0wRSFgQ%2FOH9BXlp37MIlzoDaMcVajFKZS8gcJXSICZZRdMHztkVCBW4DNZa%2BJxOwJ1E6Q6vzyyBGsc%2FRoV6Xba79A94ndoN8bYy6WNuplWyrl3mWsK2vv7sI6kqAQYvwZAuSo66P%2FLefOdgACsLXUvDUtMMpSN8TmoE6MAFh%2Fj9u1tN2PHd7Si9U0lR%2BPybWjyQIZhRLaPFiPA459dq%2F0DJYDW6PUXjnBlCqj2pjkDd8G%2FcrPGVCvjIs1dEukqNYRPh9wSQFFAb5Fi0wZV3zG%2F46Hc%2FeUQIprBOrsYtxFvQ1UWxSkaVfuuRD2NfJJC%2FoyWTyuxNmD0bdhukh9A21%2B1W83wJfu%2FecDVcxhN9MqXTDLroqrvl%2BRxWFnb%2BWbs%2B%2FV9bgL2cBI6AFt4X60UAEM%2FPSuYh%2FCMbaK1dtKiQLD4N%2F7kxQrpF%2BWzb6z4YOF5hW%2F4Jm%2BgH%2B0v9v9lego%2FcMGf98VdcRdShnMV0057mzmxPdKDL4AivAyKRRfnHrHbLoO1SdPlc41DKXR%2BjK0GtocjZ5j4aUAxDDnpo7rBTq1AThXMgqWLXhCysjiDb69KUDsmtirRuTrXpezLGPDfxkFp5ofIqWUR9J0yQNBLS%2BqlghqmfiGup4DJm90ED%2Bjr269Tuc2Ri4J0zfr7%2BcI1xixL%2Fy0rAQI30TJNbf1CsDGxfVixVDBG1Fkwa3KlpjjfAGAuGKaiPkPOMJuLo8YTbs28PVbbZSstkpcNX0npq661wJ6SM3c0BNDLzt2HCCzsKCoWT8MzDtnFnmY8Er2Z7ayg87lxx0%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090907Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYZNRZYJFV%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=b720f3d2263dac5174d9f180a9eb5e614b3e41a49d79c0654e5cf2db83daf8ac&hash=b75427625e4bad8f5793e591fc5f3d24775b20f57d1f3e4528dd1440c335f22e&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0162013417308516&tid=spdf-730e9d0a-0d56-4e6a-a6a4-f7f6e8e867cf&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2018},
type = {Journal Article}
}
x
Combined chemical and biotechnological production of 20βOH-NorDHCMT, a long-term metabolite of Oral-Turinabol (DHCMT)
Anabolic androgenic steroids (AAS) are misused very frequently in sport competitions as performance enhancing agents. One of the doping compounds that has been detected with increased frequency in the last few years is dehydrochloromethyltestosterone (DHCMT, 4-chloro-17β-hydroxy-17α-methylandrosta-1,4-dien-3-one; brand name Oral Turinabol). The long-term DHCMT metabolite 20βOH-NorDHCMT (4-chloro-17β-hydroxymethyl-17α-methyl-18-norandrosta-1,4,13-trien-3-one) was reported earlier to be detectable in urine samples for more than 22 days after DHCMT administration; however, purified reference material was not available so far. In this study we demonstrate a successful combination of Wagner-Meerwein rearrangement of DHCMT to NorDHCMT (4-chloro-17,17-dimethyl-18-norandrosta-1,4,13-trien-3-one) and subsequent whole-cell biotransformation with a recombinant fission yeast strain expressing the human cytochrome P450 enzyme (CYP or P450) CYP21A2 for the synthesis of mg amounts of this metabolite. It was then used as reference for the analysis of a post administration urine of DHCMT. The availability of this reference compound will provide an incontestable proof for DHCMT abuse.
R. Maccari, A. Del Corso, P. Paoli, I. Adornato, G. Lori, F. Balestri, M. Cappiello, A. Naß, G. Wolber, and R. Ottana. An investigation on 4-thiazolidinone derivatives as dual inhibitors of aldose reductase and protein tyrosine phosphatase 1B, in the search for potential agents for the treatment of type 2 diabetes mellitus and its complications, Bioorg Med Chem Lett, 28(23-24):3712-3720, 2018.
Links:
[doi:10.1016/j.bmcl.2018.10.024]
[show BibTeX]
[show abstract]
x
@article{RN261,
author = {Maccari, Rosanna and Del Corso, Antonella
and Paoli, Paolo and Adornato, Ilenia and
Lori, Giulia and Balestri, Francesco and
Cappiello, Mario and Naß, Alexandra and
Wolber, Gerhard and Ottana, Rosaria},
title = {An investigation on 4-thiazolidinone
derivatives as dual inhibitors of aldose
reductase and protein tyrosine phosphatase
1B, in the search for potential agents for
the treatment of type 2 diabetes mellitus
and its complications},
journal = {Bioorganic & Medicinal Chemistry
Letters},
volume = {28},
number = {23-24},
pages = {3712-3720},
note = {Maccari, Rosanna Del Corso, Antonella
Paoli, Paolo Adornato, Ilenia Lori, Giulia
Balestri, Francesco Cappiello, Mario Nass,
Alexandra Wolber, Gerhard Ottana, Rosaria
eng Research Support, Non-U.S. Gov't
England Bioorg Med Chem Lett. 2018 Dec
15;28(23-24):3712-3720. doi:
10.1016/j.bmcl.2018.10.024. Epub 2018 Oct
15.},
abstract = {Designed multiple ligands (DMLs),
developed to modulate simultaneously a
number of selected targets involved in
etiopathogenetic mechanisms of a
multifactorial disease, such as diabetes
mellitus (DM), are considered a promising
alternative to combinations of drugs, when
monotherapy results to be unsatisfactory.
In this work, compounds 1–17 were
synthesized and in vitro evaluated as DMLs
directed to aldose reductase (AR) and
protein tyrosine phosphatase 1B (PTP1B),
two key enzymes involved in different
events which are critical for the onset
and progression of type 2 DM and related
pathologies. Out of the tested
4-thiazolidinone derivatives, compounds 12
and 16, which exhibited potent AR
inhibitory effects along with interesting
inhibition of PTP1B, can be assumed as
lead compounds to further optimize and
balance the dual inhibitory profile.
Moreover, several structural portions were
identified as features that could be
useful to achieve simultaneous inhibition
of both human AR and PTP1B through binding
to non-catalytic regions of both target
enzymes.},
keywords = {Diabetes mellitus Designed multiple
ligands 4-Thiazolidinone derivatives
Aldose reductase Protein tyrosine
phosphatase 1B},
ISSN = {0960-894X},
DOI = {10.1016/j.bmcl.2018.10.024},
url = {http://www.sciencedirect.com/science/article/pii/S0960894X18308254
https://pdf.sciencedirectassets.com/271398/1-s2.0-S0960894X18X00225/1-s2.0-S0960894X18308254/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIBfvzJJhZ1Mm5mwy0J3B5VMuELdFjoz7sIRqN8wYSzx3AiEA5SeL7MgEjFO0B9r5FrlpUN4T0hArwMRoS5vGU9JFDzkq2gMIQhACGgwwNTkwMDM1NDY4NjUiDBOT17U1MUqycs1%2FKCq3AxGHFD6W1QLBSzH%2FhBU3v8JJteKuM%2BMlhBDgx9HJr06UFb8BZd%2BHmmbs1ur5t%2FlqtRCT5APQ%2Fzl6M%2FIM8%2F6YwmMYXt9Jyw268%2FtZeBsyIAjhb9UY49j0c7v6aVzNad%2FGYTpVso5RHp%2FRIuM5L67h7pTwnakyTSI6BnpaJqI2B9kWP4JEsZprxWxTmvQw9wKd%2BZ%2Fa4mZjyJSBse7DFly8%2FGnCKXIXFbkL7gEHIAamV19AyDb7zMu0NjKSYkVhnMKdyKtcoydss0kNGXNm7HiEVwqeBYZLCKy4Gr96z9kBNqkcZ%2BCvqAOmMj10aPUlpkLbmx197FcTVJ8VImn9RLlCJRDmDXNX5yS0%2B73PI%2BEazQ5%2BwF1N29hqIzLnT1Cc5utmeZ0G5AMefCBRfmgg6E2TbmfR7Ww0V5jCzNNuAslQXXlpfPN3WJ9keKLw21X%2BSCqBPm%2Fu6Xis2DUor4OC8wohPM2ZjGByx3Gqx2LFJIPCZN3cJyL1gxaivo%2BET3s3jVm6OY0z8nVa20tLJ7Fw2PK6UzrCG3VQ%2F1Wx6deq5BIg0CjfaQgBifcwYqv%2F%2F9ZMxV6ugcNNvcoHxYgw6rmO6wU6tAGkBGWe9a6585563qyrPRyFqAu%2FM2418LDCloFTRDRH9weZ%2BgHQW1B1m%2F%2FVbRWsTdBwvnU%2BOX7Dk7jsNsnZIzAffqBskxE32pvB9%2BEG%2Fi%2F0HR72BRBiQS0zSE42%2F3v4dfwbryhRJ%2FbSj6%2BtUzOYJwiXE%2BTFI6%2Bw6by7InD6OwR0E1IdegTjGtbvA15LPpug9CTJY%2FTVHEISFL2jcir2FrGkZbfPHhgSZLoAg3rn1UL%2Buq%2FcLM4%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090911Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYRDRFHOHF%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=feaf53b1f39ea803adab835418ba489cae4c6b949016e91c0fc74405f2220c83&hash=ec623c4bd4a7063e8d68152bb19a69be7b6f0203d7e5e52fe53b81ddea1e14ee&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0960894X18308254&tid=spdf-441f1cff-7ad6-4594-91a9-39f8bcc7b983&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2018},
type = {Journal Article}
}
x
An investigation on 4-thiazolidinone derivatives as dual inhibitors of aldose reductase and protein tyrosine phosphatase 1B, in the search for potential agents for the treatment of type 2 diabetes mellitus and its complications
Designed multiple ligands (DMLs), developed to modulate simultaneously a number of selected targets involved in etiopathogenetic mechanisms of a multifactorial disease, such as diabetes mellitus (DM), are considered a promising alternative to combinations of drugs, when monotherapy results to be unsatisfactory. In this work, compounds 1–17 were synthesized and in vitro evaluated as DMLs directed to aldose reductase (AR) and protein tyrosine phosphatase 1B (PTP1B), two key enzymes involved in different events which are critical for the onset and progression of type 2 DM and related pathologies. Out of the tested 4-thiazolidinone derivatives, compounds 12 and 16, which exhibited potent AR inhibitory effects along with interesting inhibition of PTP1B, can be assumed as lead compounds to further optimize and balance the dual inhibitory profile. Moreover, several structural portions were identified as features that could be useful to achieve simultaneous inhibition of both human AR and PTP1B through binding to non-catalytic regions of both target enzymes.
R. Maccari, R. Ettari, I. Adornato, A. Naß, G. Wolber, A. Bitto, F. Mannino, F. Aliquo, G. Bruno, F. Nicolo, S. Previti, S. Grasso, M. Zappala, and R. Ottana. Identification of 2-thioxoimidazolidin-4-one derivatives as novel noncovalent proteasome and immunoproteasome inhibitors, Bioorg Med Chem Lett, 28(3):278-283, 2018.
Links:
[doi:10.1016/j.bmcl.2017.12.053]
[show BibTeX]
[show abstract]
x
@article{RN251,
author = {Maccari, Rosanna and Ettari, Roberta and
Adornato, Ilenia and Naß, Alexandra and
Wolber, Gerhard and Bitto, Alessandra and
Mannino, Federica and Aliquo, Federica and
Bruno, Giuseppe and Nicolo, Francesco and
Previti, Santo and Grasso, Silvana and
Zappala, Maria and Ottana, Rosaria},
title = {Identification of
2-thioxoimidazolidin-4-one derivatives as
novel noncovalent proteasome and
immunoproteasome inhibitors},
journal = {Bioorganic & Medicinal Chemistry
Letters},
volume = {28},
number = {3},
pages = {278-283},
abstract = {This paper describes the design,
synthesis, and biological evaluation of
2-thioxoimidazolidin-4-one derivatives as
inhibitors of proteasome and
immunoproteasome, potential targets for
the treatment of hematological
malignancies. In particular, we focused
our efforts on the design of noncovalent
inhibitors, which might be a promising
therapeutic option potentially devoid of
drawbacks and side-effects related to
irreversible inhibition. Among all the
synthesized compounds, we identified a
panel of active inhibitors with Ki values
towards one or two chymotrypsin-like
activities of proteasome (β5c) and
immunoproteasome (β5i and β1i subunits)
in the low micromolar range. Docking
studies suggested a unique binding mode of
the molecules in the catalytic site of
immunoproteasome proteolytic subunits.},
keywords = {Proteasome Immunoproteasome
5-Arylidene-2-thioxoimidazolidin-4-ones
Non-covalent inhibitors Docking studies},
ISSN = {0960-894X},
DOI = {10.1016/j.bmcl.2017.12.053},
url = {http://www.sciencedirect.com/science/article/pii/S0960894X17312271
https://www.sciencedirect.com/science/article/pii/S0960894X17312271?via%3Dihub
https://pdf.sciencedirectassets.com/271398/1-s2.0-S0960894X18X0002X/1-s2.0-S0960894X17312271/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIQDpMFAxqO8i5cWwO8J2bidQOOPWWOgX2xYgjlCqnmYpKAIgMz6zEqJOOqjcysWG%2F%2BjDZIPEG4hPysl42TQ%2BxrkJgLgq2gMIQRACGgwwNTkwMDM1NDY4NjUiDMbVMeQJp%2BA6xuTeeiq3A5yGUmiSxbecX6zyK9C0u9d8FvveGrkPLG2bgf%2FLpoQrYo2WyvA%2BBWqUubZ%2F%2F2lzFcSTpeDFbT3qjC0N3gXJAnlrhfnB4HtdM5JkFOs7rk4T5uxWa2QB%2Bxrb0uGWp6C%2FKniuq6eBMiJ59EW8Qua9SC1HjapOsfuus9g5HoQTBdya%2B%2B93vPUyT1l6n3DvNF79aTePLdf4GzCoc3La3CqQwXZ%2Bv00XVVkSLXyHqEtn%2BKj4RqGYDx5vybmCYgNtuQnt%2F4u%2FKij1yfRzcl%2BKOheRiB2UBQBFkx%2BGRL%2BqBFLx23kYZU2PrVD4ZjQMMXyglfn7R41W1Ts944jcTs7eKtPEaIuwbGZFi3rfxU%2BBL%2BrhXsqDJMbPTE%2BrqYpk6xiwMVJaY6kJH62kCLazVZrQ49cjhJjxeJti4aqVRxjr4XOP%2FJ9EatP%2BZoD3q47yNzrS1dG3FcQOp6Rtx1LGD9QPU8GrLpoB2%2F%2BrydWuCVqCFWw5iNbyp05sWQsfmSpFEMzsvkKO%2FCw0bATpkHdkF6WOn0A1lXIOsI0cDUPAe1xWJ3L0KZySQ0UZUqXJ8Gw4v%2FTlL5BY%2FUxSnor26xYwn6aO6wU6tAHBqopuW5pcz%2FJI%2BrCyUdYGVL8ZGUcN9osxZNHlsdHl%2FPSb5Ey3HqdiHTks1pszrFK8F0ZxsQNCYajkgKT3SAzhgrTmaNYfXyzLVkl3rMbW5QA0SqgsnR79JfHgUXuHGBnWyrsVF5mu9vmFjpTB4j2VqmtMOIjghMdHO%2FOIcR59ey2VTJQKE1Ulq0WJqECO72nl8nHMFEUoBLcWlCu5cwJlo5NxAcL7qC1hNPBzJlNFYMqU07s%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090916Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY5J43SP76%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=e7c2580b1bb9072e94b21c17a17e77638c77cc9426365c1d5789e72f4ef95b81&hash=61d4515aaf52ead90206956d3b9b1b4f62c4062ab11c5f1b52d8f8dd8b4e4eb5&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0960894X17312271&tid=spdf-2602928c-1628-4f42-ac59-ecd3a11225cf&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2018},
type = {Journal Article}
}
x
Identification of 2-thioxoimidazolidin-4-one derivatives as novel noncovalent proteasome and immunoproteasome inhibitors
This paper describes the design, synthesis, and biological evaluation of 2-thioxoimidazolidin-4-one derivatives as inhibitors of proteasome and immunoproteasome, potential targets for the treatment of hematological malignancies. In particular, we focused our efforts on the design of noncovalent inhibitors, which might be a promising therapeutic option potentially devoid of drawbacks and side-effects related to irreversible inhibition. Among all the synthesized compounds, we identified a panel of active inhibitors with Ki values towards one or two chymotrypsin-like activities of proteasome (β5c) and immunoproteasome (β5i and β1i subunits) in the low micromolar range. Docking studies suggested a unique binding mode of the molecules in the catalytic site of immunoproteasome proteolytic subunits.
D. Schaller, M. G. Gündüz, F. X. Zhang, G. W. Zamponi, and G. Wolber. Binding mechanism investigations guiding the synthesis of novel condensed 1,4-dihydropyridine derivatives with L-/T-type calcium channel blocking activity, Eur J Med Chem, 155:1-12, 2018.
Links:
[doi:10.1016/j.ejmech.2018.05.032]
[show BibTeX]
[show abstract]
x
@article{RN258,
author = {Schaller, David and Gündüz, Miyase
Gözde and Zhang, Fang Xiong and Zamponi,
Gerald W. and Wolber, Gerhard},
title = {Binding mechanism investigations guiding
the synthesis of novel condensed
1,4-dihydropyridine derivatives with
L-/T-type calcium channel blocking
activity},
journal = {European Journal of Medicinal Chemistry},
volume = {155},
pages = {1-12},
abstract = {Nifedipine and isradipine are prominent
examples of calcium channel blockers with
a 1,4-dihydropyridine (DHP) scaffold.
Although successfully used in clinics
since decades for the treatment of
hypertension, the binding mechanism to
their target, the L-type voltage-gated
calcium channel Cav1.2, is still
incompletely understood. Recently, novel
DHP derivatives with a condensed ring
system have been discovered that show
distinct selectivity profiles to different
calcium channel subtypes. This property
renders this DHP class as a promising tool
to achieve selectivity towards distinct
calcium channel subtypes. In this study,
we identified a common binding mode for
prominent DHPs nifedipine and isradipine
using docking and pharmacophore analysis
that is also able to explain the
structure-activity relationship of a small
subseries of DHP derivatives with a
condensed ring system. These findings were
used to guide the synthesis of twenty-two
novel DHPs. An extensive characterization
using 1H NMR, 13C NMR, mass spectra and
elemental analysis was followed by whole
cell patch clamp assays for analyzing
activity at Cav1.2 and Cav3.2. Two
compounds were identified with significant
activity against Cav1.2. Additionally, we
identified four compounds active against
Cav3.2 of which three were selective over
Cav1.2. Novel binding modes were analyzed
using docking and pharmacophore analysis
as well as molecular dynamics
simulations.},
keywords = {1,4-dihydropyridine Hexahydroquinoline
Calcium channel Whole cell patch clamp
Binding mechanism Molecular modelling},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2018.05.032},
url = {https://www.sciencedirect.com/science/article/pii/S0223523418304458
https://pdf.sciencedirectassets.com/271932/1-s2.0-S0223523418X00129/1-s2.0-S0223523418304458/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIQDpMFAxqO8i5cWwO8J2bidQOOPWWOgX2xYgjlCqnmYpKAIgMz6zEqJOOqjcysWG%2F%2BjDZIPEG4hPysl42TQ%2BxrkJgLgq2gMIQRACGgwwNTkwMDM1NDY4NjUiDMbVMeQJp%2BA6xuTeeiq3A5yGUmiSxbecX6zyK9C0u9d8FvveGrkPLG2bgf%2FLpoQrYo2WyvA%2BBWqUubZ%2F%2F2lzFcSTpeDFbT3qjC0N3gXJAnlrhfnB4HtdM5JkFOs7rk4T5uxWa2QB%2Bxrb0uGWp6C%2FKniuq6eBMiJ59EW8Qua9SC1HjapOsfuus9g5HoQTBdya%2B%2B93vPUyT1l6n3DvNF79aTePLdf4GzCoc3La3CqQwXZ%2Bv00XVVkSLXyHqEtn%2BKj4RqGYDx5vybmCYgNtuQnt%2F4u%2FKij1yfRzcl%2BKOheRiB2UBQBFkx%2BGRL%2BqBFLx23kYZU2PrVD4ZjQMMXyglfn7R41W1Ts944jcTs7eKtPEaIuwbGZFi3rfxU%2BBL%2BrhXsqDJMbPTE%2BrqYpk6xiwMVJaY6kJH62kCLazVZrQ49cjhJjxeJti4aqVRxjr4XOP%2FJ9EatP%2BZoD3q47yNzrS1dG3FcQOp6Rtx1LGD9QPU8GrLpoB2%2F%2BrydWuCVqCFWw5iNbyp05sWQsfmSpFEMzsvkKO%2FCw0bATpkHdkF6WOn0A1lXIOsI0cDUPAe1xWJ3L0KZySQ0UZUqXJ8Gw4v%2FTlL5BY%2FUxSnor26xYwn6aO6wU6tAHBqopuW5pcz%2FJI%2BrCyUdYGVL8ZGUcN9osxZNHlsdHl%2FPSb5Ey3HqdiHTks1pszrFK8F0ZxsQNCYajkgKT3SAzhgrTmaNYfXyzLVkl3rMbW5QA0SqgsnR79JfHgUXuHGBnWyrsVF5mu9vmFjpTB4j2VqmtMOIjghMdHO%2FOIcR59ey2VTJQKE1Ulq0WJqECO72nl8nHMFEUoBLcWlCu5cwJlo5NxAcL7qC1hNPBzJlNFYMqU07s%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090934Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY5J43SP76%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=c849855703227baf4068983f2ec0fd8823703c021692f5938ab30d1eb2fa9bff&hash=2b4e25a1b06c86632253879a8372204f4f5ae2a04e12ae7231cd5b4a48705142&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0223523418304458&tid=spdf-307057ac-8507-41f8-9a2e-60eca516f2bb&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2018},
type = {Journal Article}
}
x
Binding mechanism investigations guiding the synthesis of novel condensed 1,4-dihydropyridine derivatives with L-/T-type calcium channel blocking activity
Nifedipine and isradipine are prominent examples of calcium channel blockers with a 1,4-dihydropyridine (DHP) scaffold. Although successfully used in clinics since decades for the treatment of hypertension, the binding mechanism to their target, the L-type voltage-gated calcium channel Cav1.2, is still incompletely understood. Recently, novel DHP derivatives with a condensed ring system have been discovered that show distinct selectivity profiles to different calcium channel subtypes. This property renders this DHP class as a promising tool to achieve selectivity towards distinct calcium channel subtypes. In this study, we identified a common binding mode for prominent DHPs nifedipine and isradipine using docking and pharmacophore analysis that is also able to explain the structure-activity relationship of a small subseries of DHP derivatives with a condensed ring system. These findings were used to guide the synthesis of twenty-two novel DHPs. An extensive characterization using 1H NMR, 13C NMR, mass spectra and elemental analysis was followed by whole cell patch clamp assays for analyzing activity at Cav1.2 and Cav3.2. Two compounds were identified with significant activity against Cav1.2. Additionally, we identified four compounds active against Cav3.2 of which three were selective over Cav1.2. Novel binding modes were analyzed using docking and pharmacophore analysis as well as molecular dynamics simulations.
R. Schulz, A. Atef, D. Becker, F. Gottschalk, C. Tauber, S. Wagner, C. Arkona, A. A. Abdel-Hafez, H. H. Farag, J. Rademann, and G. Wolber. Phenylthiomethyl ketone-based fragments show selective and irreversible inhibition of enteroviral 3C proteases, J Med Chem, 61(3):1218-1230, 2018.
Links:
[doi:10.1021/acs.jmedchem.7b01440]
[show BibTeX]
[show abstract]
x
@article{RN252,
author = {Schulz, Robert and Atef, Amira and
Becker, Daniel and Gottschalk, Franziska
and Tauber, Carolin and Wagner, Stefan and
Arkona, Christoph and Abdel-Hafez, Atef A.
and Farag, Hassan H. and Rademann, Jörg
and Wolber, Gerhard},
title = {Phenylthiomethyl ketone-based fragments
show selective and irreversible inhibition
of enteroviral 3C proteases},
journal = {Journal of Medicinal Chemistry},
volume = {61},
number = {3},
pages = {1218-1230},
abstract = {Lead structure discovery mainly focuses
on the identification of noncovalently
binding ligands. Covalent linkage,
however, is an essential binding mechanism
for a multitude of successfully marketed
drugs although discovered by serendipity
in most cases. We present a concept for
the design of fragments covalently binding
to proteases. Covalent linkage enables
fragment binding unrelated to affinity to
shallow protein binding sites and at the
same time allows differentiated targeted
hit verification and binding location
verification through mass spectrometry. We
describe a systematic and rational
computational approach for the
identification of covalently binding
fragments from compound collections
inhibiting enteroviral 3C protease, a
target with high therapeutic potential. By
implementing reactive groups potentially
forming covalent bonds as chemical feature
in our 3D pharmacophore methodology,
covalent binders were discovered by
high-throughput virtual screening. We
present careful experimental validation of
the virtual hits using enzymatic assays
and mass spectrometry unraveling a novel,
previously unknown irreversible inhibition
of the 3C protease by phenylthiomethyl
ketone-based fragments. Subsequent
synthetic optimization through fragment
growing and reactivity analysis against
catalytic and non-catalytic cysteines
revealed specific irreversible 3C protease
inhibition.},
ISSN = {0022-2623},
DOI = {10.1021/acs.jmedchem.7b01440},
url = {http://dx.doi.org/10.1021/acs.jmedchem.7b01440
https://pubs.acs.org/doi/pdfplus/10.1021/acs.jmedchem.7b01440},
year = {2018},
type = {Journal Article}
}
x
Phenylthiomethyl ketone-based fragments show selective and irreversible inhibition of enteroviral 3C proteases
Lead structure discovery mainly focuses on the identification of noncovalently binding ligands. Covalent linkage, however, is an essential binding mechanism for a multitude of successfully marketed drugs although discovered by serendipity in most cases. We present a concept for the design of fragments covalently binding to proteases. Covalent linkage enables fragment binding unrelated to affinity to shallow protein binding sites and at the same time allows differentiated targeted hit verification and binding location verification through mass spectrometry. We describe a systematic and rational computational approach for the identification of covalently binding fragments from compound collections inhibiting enteroviral 3C protease, a target with high therapeutic potential. By implementing reactive groups potentially forming covalent bonds as chemical feature in our 3D pharmacophore methodology, covalent binders were discovered by high-throughput virtual screening. We present careful experimental validation of the virtual hits using enzymatic assays and mass spectrometry unraveling a novel, previously unknown irreversible inhibition of the 3C protease by phenylthiomethyl ketone-based fragments. Subsequent synthetic optimization through fragment growing and reactivity analysis against catalytic and non-catalytic cysteines revealed specific irreversible 3C protease inhibition.
A. Tkachenko, M. Bermudez, S. Irmer-Stooff, D. Genkinger, F. Henkler-Stephani, G. Wolber, and A. Luch. Nuclear transport of the human aryl hydrocarbon receptor and subsequent gene induction relies on its residue histidine 291, Arch Toxicol, 92(3):1151-1160, 2018.
Links:
[doi:10.1007/s00204-017-2129-0]
[show BibTeX]
[show abstract]
x
@article{RN249,
author = {Tkachenko, A. and Bermudez, M. and
Irmer-Stooff, S. and Genkinger, D. and
Henkler-Stephani, F. and Wolber, G. and
Luch, A.},
title = {Nuclear transport of the human aryl
hydrocarbon receptor and subsequent gene
induction relies on its residue histidine
291},
journal = {Archives of Toxicology},
volume = {92},
number = {3},
pages = {1151-1160},
abstract = {The aryl hydrocarbon receptor (AHR) is a
ligand-dependent transcription factor
involved in the metabolism of
physiological substances and xenobiotics,
representing an interesting target in both
toxicology and pharmacology. In this
study, we investigated the
ligand-dependent conjunction of nuclear
import of the human AHR in living cells
and target gene induction. Our findings
strengthen the theory that the AHR
triggers a precisely defined and rapid
reaction upon binding to endogenous
ligands, while the xenobiotic
β-naphthoflavone only induces rather
unspecific and slow effects. To better
illuminate the ligand-mediated responses
of the human AHR, we applied site-directed
mutagenesis and identified histidine 291
as key residue for AHR functionality,
essential for both nuclear import and
target gene induction. Contrary, replacing
histidine at position 291 by alanine did
not affect nucleo-cytoplasmic shuttling,
showing that permanent endogenous import
and ligand-induced import of the AHR into
the nucleus are two independent and
differently regulated processes. Combining
these observations with our structural
investigations using a homology model of
the AHR-PAS B domain, we suggest a dual
role of histidine 291: (1) a major role
for shaping the ligand binding site
including direct interactions with ligands
and, (2) an essential role for the
conformational dynamics of a PAS B loop,
which most likely influences the
association of the AHR with the AHR
nuclear translocator through interference
with their protein–protein interface.},
ISSN = {1432-0738},
DOI = {10.1007/s00204-017-2129-0},
url = {https://doi.org/10.1007/s00204-017-2129-0
https://link.springer.com/content/pdf/10.1007%2Fs00204-017-2129-0.pdf},
year = {2018},
type = {Journal Article}
}
x
Nuclear transport of the human aryl hydrocarbon receptor and subsequent gene induction relies on its residue histidine 291
The aryl hydrocarbon receptor (AHR) is a ligand-dependent transcription factor involved in the metabolism of physiological substances and xenobiotics, representing an interesting target in both toxicology and pharmacology. In this study, we investigated the ligand-dependent conjunction of nuclear import of the human AHR in living cells and target gene induction. Our findings strengthen the theory that the AHR triggers a precisely defined and rapid reaction upon binding to endogenous ligands, while the xenobiotic β-naphthoflavone only induces rather unspecific and slow effects. To better illuminate the ligand-mediated responses of the human AHR, we applied site-directed mutagenesis and identified histidine 291 as key residue for AHR functionality, essential for both nuclear import and target gene induction. Contrary, replacing histidine at position 291 by alanine did not affect nucleo-cytoplasmic shuttling, showing that permanent endogenous import and ligand-induced import of the AHR into the nucleus are two independent and differently regulated processes. Combining these observations with our structural investigations using a homology model of the AHR-PAS B domain, we suggest a dual role of histidine 291: (1) a major role for shaping the ligand binding site including direct interactions with ligands and, (2) an essential role for the conformational dynamics of a PAS B loop, which most likely influences the association of the AHR with the AHR nuclear translocator through interference with their protein–protein interface.
F. Yang, D. Machalz, S. Wang, Z. Li, G. Wolber, and M. Bureik. A common polymorphic variant of UGT1A5 displays increased activity due to optimized cofactor binding, Febs Lett, 592(11):1837-1846, 2018.
Links:
[doi:10.1002/1873-3468.13072]
[show BibTeX]
[show abstract]
x
@article{RN259,
author = {Yang, Fan and Machalz, David and Wang,
Sisi and Li, Zhengyi and Wolber, Gerhard
and Bureik, Matthias},
title = {A common polymorphic variant of UGT1A5
displays increased activity due to
optimized cofactor binding},
journal = {FEBS Letters},
volume = {592},
number = {11},
pages = {1837-1846},
abstract = {Uridine
diphosphate‐glucuronosyltransferases
(UGTs) are the most important phase II
enzymes in human drug metabolism. Using
permeabilized recombinant fission yeast
cells (enzyme bags), we demonstrate that
UGT1A5 can catalyze an N‐glucuronidation
reaction. We characterized two new
polymorphic UGT1A5 variants: a common
ninefold mutant (UGT1A5*8) with
double‐fold activity and a much rarer
sixfold mutant (UGT1A5*9), which has the
same activity as the wild‐type.
Molecular modeling studies indicate that
the minor effects of all mutations, except
for Gly259Arg, are due to their distance
to the substrate binding site. Extensive
molecular dynamics simulations revealed
that the Gly259Arg mutation stabilizes
helix Q through a newly formed hydrogen
bonding network, which places the cofactor
in a much more favorable geometry in
UGT1A5*8 as compared to the wild‐type.},
DOI = {10.1002/1873-3468.13072},
url = {https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1873-3468.13072
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1002/1873-3468.13072},
year = {2018},
type = {Journal Article}
}
x
A common polymorphic variant of UGT1A5 displays increased activity due to optimized cofactor binding
Uridine diphosphate‐glucuronosyltransferases (UGTs) are the most important phase II enzymes in human drug metabolism. Using permeabilized recombinant fission yeast cells (enzyme bags), we demonstrate that UGT1A5 can catalyze an N‐glucuronidation reaction. We characterized two new polymorphic UGT1A5 variants: a common ninefold mutant (UGT1A5*8) with double‐fold activity and a much rarer sixfold mutant (UGT1A5*9), which has the same activity as the wild‐type. Molecular modeling studies indicate that the minor effects of all mutations, except for Gly259Arg, are due to their distance to the substrate binding site. Extensive molecular dynamics simulations revealed that the Gly259Arg mutation stabilizes helix Q through a newly formed hydrogen bonding network, which places the cofactor in a much more favorable geometry in UGT1A5*8 as compared to the wild‐type.
H. A. Abuelizz, R. Al-Salahi, J. Al-Asri, J. Mortier, M. Marzouk, E. Ezzeldin, A. A. Ali, M. G. Khalil, G. Wolber, H. A. Ghabbour, A. A. Almehizia, and G. A. Abdel Jaleel. Synthesis, crystallographic characterization, molecular docking and biological activity of isoquinoline derivatives, Chem Cent J, 11(1):103, 2017.
Links:
[doi:10.1186/s13065-017-0321-1]
[show BibTeX]
[show abstract]
x
@article{RN207,
author = {Abuelizz, Hatem A. and Al-Salahi, Rashad
and Al-Asri, Jamil and Mortier, Jérémie
and Marzouk, Mohamed and Ezzeldin, Essam
and Ali, Azza A. and Khalil, Mona G. and
Wolber, Gerhard and Ghabbour, Hazem A. and
Almehizia, Abdulrahman A. and Abdel
Jaleel, Gehad A.},
title = {Synthesis, crystallographic
characterization, molecular docking and
biological activity of isoquinoline
derivatives},
journal = {Chemistry Central Journal},
volume = {11},
number = {1},
pages = {103},
abstract = {The main objective of this work was to
synthesize novel compounds with a
benzo[de][1,2,4]triazolo[5,1-a]isoquinoline
scaffold by employing
(dioxo-benzo[de]isoquinolin-2-yl) thiourea
as a building block. Molecular docking was
conducted in the COX-2 active site to
predict the plausible binding mode and
rationalize the structure–activity
relationship of the synthesized compounds.
The structures of the synthesized
compounds were confirmed by HREI-MS, and
NMR spectra along with X-ray diffraction
were collected for products 1 and 5.
Thereafter, anti-inflammatory effect of
molecules 1–20 was evaluated in vivo
using carrageenan-induced paw edema
method, revealing significant inhibition
potency in albino rats with an activity
comparable to that of the standard drugs
indomethacin. Compounds 8, 9, 15 and 16
showed the highest anti-inflammatory
activity. However, thermal sensitivity-hot
plat test, a radiological examination and
motor coordination assessment were
performed to test the activity against
rheumatoid arthritis. The obtained results
indicate promising anti-arthritic activity
for compounds 9 and 15 as significant
reduction of the serum level of
interleukin-1β [IL-1β], cyclooxygenase-2
[COX-2] and prostaglandin E2 [PGE2] was
observed in CFA rats.},
ISSN = {1752-153X},
DOI = {10.1186/s13065-017-0321-1},
url = {https://doi.org/10.1186/s13065-017-0321-1
https://ccj.springeropen.com/track/pdf/10.1186/s13065-017-0321-1?site= ccj.springeropen.com},
year = {2017},
type = {Journal Article}
}
x
Synthesis, crystallographic characterization, molecular docking and biological activity of isoquinoline derivatives
The main objective of this work was to synthesize novel compounds with a benzo[de][1,2,4]triazolo[5,1-a]isoquinoline scaffold by employing (dioxo-benzo[de]isoquinolin-2-yl) thiourea as a building block. Molecular docking was conducted in the COX-2 active site to predict the plausible binding mode and rationalize the structure–activity relationship of the synthesized compounds. The structures of the synthesized compounds were confirmed by HREI-MS, and NMR spectra along with X-ray diffraction were collected for products 1 and 5. Thereafter, anti-inflammatory effect of molecules 1–20 was evaluated in vivo using carrageenan-induced paw edema method, revealing significant inhibition potency in albino rats with an activity comparable to that of the standard drugs indomethacin. Compounds 8, 9, 15 and 16 showed the highest anti-inflammatory activity. However, thermal sensitivity-hot plat test, a radiological examination and motor coordination assessment were performed to test the activity against rheumatoid arthritis. The obtained results indicate promising anti-arthritic activity for compounds 9 and 15 as significant reduction of the serum level of interleukin-1β [IL-1β], cyclooxygenase-2 [COX-2] and prostaglandin E2 [PGE2] was observed in CFA rats.
M. Bermudez, A. Bock, F. Krebs, U. Holzgrabe, K. Mohr, M. J. Lohse, and G. Wolber. Ligand-specific restriction of extracellular conformational dynamics constrains signaling of the M2 muscarinic receptor, ACS Chem Biol, 12(7):1743-1748, 2017.
Links:
[doi:10.1021/acschembio.7b00275]
[show BibTeX]
[show abstract]
x
@article{RN203,
author = {Bermudez, Marcel and Bock, Andreas and
Krebs, Fabian and Holzgrabe, Ulrike and
Mohr, Klaus and Lohse, Martin J. and
Wolber, Gerhard},
title = {Ligand-specific restriction of
extracellular conformational dynamics
constrains signaling of the M2 muscarinic
receptor},
journal = {ACS Chemical Biology},
volume = {12},
number = {7},
pages = {1743-1748},
abstract = {G protein-coupled receptors transmit
extracellular signals across cell
membranes via different G protein classes
and β-arrestins. Some pathways may be
therapeutically beneficial, whereas others
may be detrimental under certain
pathophysiological conditions. For many
GPCRs, biased agonists are available,
which preferentially signal through one
pathway or a subset of pathways, and
harnessing biased agonism could be a
potential novel therapeutic strategy.
However, the incomplete mechanistic
understanding of biased agonism hampers
rational design of biased ligands. Using
the muscarinic M2 receptor as a model
system, we have analyzed the relationship
between ligand-dependent conformational
changes as revealed in all-atom MD
simulations and the activation of specific
G proteins. We find that the extent of
closure of the extracellular, allosteric
binding site interferes with the
activation of certain G proteins. Our data
allow the rational design of Gi-biased
agonists at the M2 receptor and delineate
a simple principle which may be translated
to other GPRCs.},
keywords = {gpcr},
DOI = {10.1021/acschembio.7b00275},
url = {http://pubs.acs.org/doi/pdfplus/10.1021/acschembio.7b00275},
year = {2017},
type = {Journal Article}
}
x
Ligand-specific restriction of extracellular conformational dynamics constrains signaling of the M2 muscarinic receptor
G protein-coupled receptors transmit extracellular signals across cell membranes via different G protein classes and β-arrestins. Some pathways may be therapeutically beneficial, whereas others may be detrimental under certain pathophysiological conditions. For many GPCRs, biased agonists are available, which preferentially signal through one pathway or a subset of pathways, and harnessing biased agonism could be a potential novel therapeutic strategy. However, the incomplete mechanistic understanding of biased agonism hampers rational design of biased ligands. Using the muscarinic M2 receptor as a model system, we have analyzed the relationship between ligand-dependent conformational changes as revealed in all-atom MD simulations and the activation of specific G proteins. We find that the extent of closure of the extracellular, allosteric binding site interferes with the activation of certain G proteins. Our data allow the rational design of Gi-biased agonists at the M2 receptor and delineate a simple principle which may be translated to other GPRCs.
J. Mortier, J. R. C. Prévost, D. Sydow, S. Teuchert, C. Omieczynski, M. Bermudez, R. Frédérick, and G. Wolber. Arginase structure and inhibition: Catalytic site plasticity reveals new modulation possibilities, Scientific Reports, 7(1):13616, 2017.
Links:
[doi:10.1038/s41598-017-13366-4]
[show BibTeX]
[show abstract]
x
@article{RN248,
author = {Mortier, Jérémie and Prévost, Julien
R. C. and Sydow, Dominique and Teuchert,
Sabine and Omieczynski, Christian and
Bermudez, Marcel and Frédérick, Raphaël
and Wolber, Gerhard},
title = {Arginase structure and inhibition:
Catalytic site plasticity reveals new
modulation possibilities},
journal = {Scientific Reports},
volume = {7},
number = {1},
pages = {13616},
abstract = {Metalloenzyme arginase is a
therapeutically relevant target associated
with tumor growth. To fight cancer
immunosuppression, arginase activity can
be modulated by small chemical inhibitors
binding to its catalytic center. To better
understand molecular mechanisms of
arginase inhibition, a careful
computer-aided mechanistic structural
investigation of this enzyme was
conducted. Using molecular dynamics (MD)
simulations in the microsecond range, key
regions of the protein active site were
identified and their flexibility was
evaluated and compared. A cavity opening
phenomenon was observed, involving three
loops directly interacting with all known
ligands, while metal coordinating regions
remained motionless. A novel dynamic 3D
pharmacophore analysis method termed
dynophores has been developed that allows
for the construction of a single 3D-model
comprising all ligand-enzyme interactions
occurring throughout a complete MD
trajectory. This new technique for the in
silico study of intermolecular
interactions allows for loop flexibility
analysis coupled with movements and
conformational changes of bound ligands.
Presented MD studies highlight the
plasticity of the size of the arginase
active site, leading to the hypothesis
that larger ligands can enter the cavity
of arginase. Experimental testing of a
targeted fragment library substituted by
different aliphatic groups validates this
hypothesis, paving the way for the design
of arginase inhibitors with novel binding
patterns.},
keywords = {dynophore},
ISSN = {2045-2322},
DOI = {10.1038/s41598-017-13366-4},
url = {https://doi.org/10.1038/s41598-017-13366-4
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5648838/pdf/41598_2017_Article_13366.pdf},
year = {2017},
type = {Journal Article}
}
x
Arginase structure and inhibition: Catalytic site plasticity reveals new modulation possibilities
Metalloenzyme arginase is a therapeutically relevant target associated with tumor growth. To fight cancer immunosuppression, arginase activity can be modulated by small chemical inhibitors binding to its catalytic center. To better understand molecular mechanisms of arginase inhibition, a careful computer-aided mechanistic structural investigation of this enzyme was conducted. Using molecular dynamics (MD) simulations in the microsecond range, key regions of the protein active site were identified and their flexibility was evaluated and compared. A cavity opening phenomenon was observed, involving three loops directly interacting with all known ligands, while metal coordinating regions remained motionless. A novel dynamic 3D pharmacophore analysis method termed dynophores has been developed that allows for the construction of a single 3D-model comprising all ligand-enzyme interactions occurring throughout a complete MD trajectory. This new technique for the in silico study of intermolecular interactions allows for loop flexibility analysis coupled with movements and conformational changes of bound ligands. Presented MD studies highlight the plasticity of the size of the arginase active site, leading to the hypothesis that larger ligands can enter the cavity of arginase. Experimental testing of a targeted fragment library substituted by different aliphatic groups validates this hypothesis, paving the way for the design of arginase inhibitors with novel binding patterns.
M. S. Murgueitio, S. Ebner, P. Hörtnagl, C. Rakers, R. Bruckner, P. Henneke, G. Wolber, and S. Santos-Sierra. Enhanced immunostimulatory activity of in silico discovered agonists of Toll-like receptor 2 (TLR2), Biochimica et Biophysica Acta (BBA), General Subjects, 1861(11):2680-2689, 2017.
Links:
[doi:10.1016/j.bbagen.2017.07.011]
[show BibTeX]
[show abstract]
x
@article{RN205,
author = {Murgueitio, M. S. and Ebner, S. and
Hörtnagl, P. and Rakers, C. and Bruckner,
R. and Henneke, P. and Wolber, G. and
Santos-Sierra, S.},
title = {Enhanced immunostimulatory activity of in
silico discovered agonists of Toll-like
receptor 2 (TLR2)},
journal = {Biochimica et Biophysica Acta (BBA),
General Subjects},
volume = {1861},
number = {11},
pages = {2680-2689},
abstract = {Emergent therapies in anticancer
vaccination use Toll-like receptors (TLRs)
agonists as dendritic cell (DC) vaccine
adjuvants. DCs from the patient are
isolated, stimulated with TLR agonists and
tumor antigens ex vivo and then infused
back into the patient. Although some TLR
ligands have been tested in clinical
trials, novel TLR agonists with improved
immunomodulatory properties are essential
to optimize treatment success. We report
on the discovery of small-molecule TLR2
agonists, with favorable properties as
synthetic adjuvants. We performed a shape-
and featured-based similarity virtual
screening against a commercially available
compound library. The selected virtual
hits were experimentally tested in
TLR2-reporter cells and their activity in
phagocytes and DCs was characterized. A
binding model of the compounds to TLR2
(docking studies) was proposed. Through a
virtual screening approach against a
library of three million compounds four
virtual hits (AG1, AG2, AG3, AG4) were
found to synergistically augment the NF-kB
activation induced by the lipopeptide
ligand Pam3CSK4 in luciferase reporter
assays using HEK293-TLR2 cells. Biacore
experiments indicated that AG1–AG4 are
ago-allosteric modulators of TLR2 and AG2
bound TLR2 with high affinity (KD 0.8μM).
The compounds induced TNF-α production in
human peripheral blood mononuclear cells
(PBMCs) and they activated DCs as
indicated by IL-12 production and
upregulation of CD83/CD86. Following a
combined in silico/in vitro approach we
have discovered TLR2-agonists (AG1–AG4)
that activate human and mouse immune
cells. We introduce four novel TLR2
ago-allosteric modulators that stimulate
myeloid cell activity and constitute
promising candidates as synthetic
adjuvants.},
keywords = {Toll-like receptor Cancer vaccination
Virtual screening Adjuvants Dendritic cell
Inflammation},
ISSN = {0304-4165},
DOI = {10.1016/j.bbagen.2017.07.011},
url = {http://www.sciencedirect.com/science/article/pii/S0304416517302222
https://ac.els-cdn.com/S0304416517302222/1-s2.0-S0304416517302222-main.pdf?_tid= 0e5b1604-ba65-11e7-9947-00000aab0f27&acdnat=1509033065_be28cc321c81e00bd2ec48eb34559e40},
year = {2017},
type = {Journal Article}
}
x
Enhanced immunostimulatory activity of in silico discovered agonists of Toll-like receptor 2 (TLR2)
Emergent therapies in anticancer vaccination use Toll-like receptors (TLRs) agonists as dendritic cell (DC) vaccine adjuvants. DCs from the patient are isolated, stimulated with TLR agonists and tumor antigens ex vivo and then infused back into the patient. Although some TLR ligands have been tested in clinical trials, novel TLR agonists with improved immunomodulatory properties are essential to optimize treatment success. We report on the discovery of small-molecule TLR2 agonists, with favorable properties as synthetic adjuvants. We performed a shape- and featured-based similarity virtual screening against a commercially available compound library. The selected virtual hits were experimentally tested in TLR2-reporter cells and their activity in phagocytes and DCs was characterized. A binding model of the compounds to TLR2 (docking studies) was proposed. Through a virtual screening approach against a library of three million compounds four virtual hits (AG1, AG2, AG3, AG4) were found to synergistically augment the NF-kB activation induced by the lipopeptide ligand Pam3CSK4 in luciferase reporter assays using HEK293-TLR2 cells. Biacore experiments indicated that AG1–AG4 are ago-allosteric modulators of TLR2 and AG2 bound TLR2 with high affinity (KD 0.8μM). The compounds induced TNF-α production in human peripheral blood mononuclear cells (PBMCs) and they activated DCs as indicated by IL-12 production and upregulation of CD83/CD86. Following a combined in silico/in vitro approach we have discovered TLR2-agonists (AG1–AG4) that activate human and mouse immune cells. We introduce four novel TLR2 ago-allosteric modulators that stimulate myeloid cell activity and constitute promising candidates as synthetic adjuvants.
M. S. Murgueitio, C. Rakers, A. Frank, and G. Wolber. Balancing Inflammation: Computational Design of Small-Molecule Toll-like Receptor Modulators, Trends Pharmacol Sci, 38(2):155-168, 2017.
Links:
[doi:10.1016/j.tips.2016.10.007]
[show BibTeX]
[show abstract]
x
@article{RN201,
author = {Murgueitio, Manuela S. and Rakers,
Christin and Frank, Anne and Wolber,
Gerhard},
title = {Balancing Inflammation: Computational
Design of Small-Molecule Toll-like
Receptor Modulators},
journal = {Trends in Pharmacological Sciences},
volume = {38},
number = {2},
pages = {155-168},
abstract = {As essential proteins of the innate
immune system, Toll-like receptors (TLRs)
are involved in a plethora of
physiological pathologies and their
modulation is an ongoing quest in the
field of drug discovery. Although TLRs
recognize an unusually broad range of
different molecular patterns, only a few
small-molecule TLR modulators have been
reported to date. Recent advances in
crystallography and in silico techniques
provide promising opportunities for TLR
investigations and drug design. Here,
three application areas for computational
approaches are considered: (i) exploration
of TLR structure and activation; (ii)
understanding TLR modulation; and (iii)
TLR drug discovery. By providing an
overview on state-of-the-art computational
methods, we highlight the value of
molecular modeling in mechanistically
understanding TLR function and guiding
drug design.},
ISSN = {0165-6147},
DOI = {10.1016/j.tips.2016.10.007},
url = {http://dx.doi.org/10.1016/j.tips.2016.10.007
http://ac.els-cdn.com/S0165614716301377/1-s2.0-S0165614716301377-main.pdf?_tid= 8ea85ae0-b7c0-11e6-8b76-00000aab0f27&acdnat=1480595013_5bb0b3aaeb0d4f16e65dd6468b9b7091},
year = {2017},
type = {Journal Article}
}
x
Balancing Inflammation: Computational Design of Small-Molecule Toll-like Receptor Modulators
As essential proteins of the innate immune system, Toll-like receptors (TLRs) are involved in a plethora of physiological pathologies and their modulation is an ongoing quest in the field of drug discovery. Although TLRs recognize an unusually broad range of different molecular patterns, only a few small-molecule TLR modulators have been reported to date. Recent advances in crystallography and in silico techniques provide promising opportunities for TLR investigations and drug design. Here, three application areas for computational approaches are considered: (i) exploration of TLR structure and activation; (ii) understanding TLR modulation; and (iii) TLR drug discovery. By providing an overview on state-of-the-art computational methods, we highlight the value of molecular modeling in mechanistically understanding TLR function and guiding drug design.
R. Ottana, P. Paoli, A. Naß, G. Lori, V. Cardile, I. Adornato, A. Rotondo, A. C. Eleonora Graziano, G. Wolber, and R. Maccari. Discovery of 4-[(5-arylidene-4-oxothiazolidin-3-yl)methyl]benzoic acid derivatives active as novel potent allosteric inhibitors of protein tyrosine phosphatase 1B: In silico studies and in vitro evaluation as insulinomimetic and anti-inflammatory agents, Eur J Med Chem, 127:840-858, 2017.
Links:
[doi:10.1016/j.ejmech.2016.10.063]
[show BibTeX]
[show abstract]
x
@article{RN199,
author = {Ottana, Rosaria and Paoli, Paolo and
Naß, Alexandra and Lori, Giulia and
Cardile, Venera and Adornato, Ilenia and
Rotondo, Archimede and Eleonora Graziano,
Adriana Carol and Wolber, Gerhard and
Maccari, Rosanna},
title = {Discovery of
4-[(5-arylidene-4-oxothiazolidin-3-yl)methyl]benzoic
acid derivatives active as novel potent
allosteric inhibitors of protein tyrosine
phosphatase 1B: In silico studies and in
vitro evaluation as insulinomimetic and
anti-inflammatory agents},
journal = {European Journal of Medicinal Chemistry},
volume = {127},
pages = {840-858},
abstract = {New
4-{[5-arylidene-2-(4-fluorophenylimino)-4-oxothiazolidin-3-yl]methyl}benzoic
acids (5) and 2-thioxo-4-thiazolidinone
analogues (6) were synthesised as a part
of a continuing search for new inhibitors
of protein tyrosine phosphatase 1B
(PTP1B), an enzyme which is implicated in
metabolic disorders and inflammatory
signaling. Most of the tested compounds
were shown to be potent PTP1B inhibitors.
Moreover, their inhibition mechanism was
markedly influenced by the substituents in
the positions 2 and 5, as kinetic studies
indicated. Docking experiments suggested
that certain derivatives 5 and 6 may
efficiently fit into an allosteric site
positioned between the β-sheet including
Leu71 and Lys73 and a lipophilic pocket
closed by the loop consisting of Pro210 to
Leu 204. In cellular assays, several of
these new 4-thiazolidinone derivatives
showed insulinomimetic and
anti-inflammatory properties. Out of them,
compound 5b exhibited the most promising
profile, being able to promote the
activation of both insulin receptor and
downstream Akt protein as well as to
increase 2-deoxyglucose cellular uptake.
Interestingly, compound 5b was also able
to interrupt critical events in
inflammatory signalling.},
keywords = {Protein tyrosine phosphatases Enzyme
inhibitors Insulinomimetic effects
Anti-inflammatory activity Molecular
docking 5-Arylidene-4-thiazolidinone
derivatives},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2016.10.063},
url = {http://www.sciencedirect.com/science/article/pii/S0223523416309345
http://ac.els-cdn.com/S0223523416309345/1-s2.0-S0223523416309345-main.pdf?_tid= 72e236fe-ed3b-11e6-8bc2-00000aab0f6c&acdnat=1486475206_d6dd5764eee03f4148356c005814f688},
year = {2017},
type = {Journal Article}
}
x
Discovery of 4-[(5-arylidene-4-oxothiazolidin-3-yl)methyl]benzoic acid derivatives active as novel potent allosteric inhibitors of protein tyrosine phosphatase 1B: In silico studies and in vitro evaluation as insulinomimetic and anti-inflammatory agents
New 4-{[5-arylidene-2-(4-fluorophenylimino)-4-oxothiazolidin-3-yl]methyl}benzoic acids (5) and 2-thioxo-4-thiazolidinone analogues (6) were synthesised as a part of a continuing search for new inhibitors of protein tyrosine phosphatase 1B (PTP1B), an enzyme which is implicated in metabolic disorders and inflammatory signaling. Most of the tested compounds were shown to be potent PTP1B inhibitors. Moreover, their inhibition mechanism was markedly influenced by the substituents in the positions 2 and 5, as kinetic studies indicated. Docking experiments suggested that certain derivatives 5 and 6 may efficiently fit into an allosteric site positioned between the β-sheet including Leu71 and Lys73 and a lipophilic pocket closed by the loop consisting of Pro210 to Leu 204. In cellular assays, several of these new 4-thiazolidinone derivatives showed insulinomimetic and anti-inflammatory properties. Out of them, compound 5b exhibited the most promising profile, being able to promote the activation of both insulin receptor and downstream Akt protein as well as to increase 2-deoxyglucose cellular uptake. Interestingly, compound 5b was also able to interrupt critical events in inflammatory signalling.
F. Sanz, F. Pognan, T. Steger-Hartmann, C. Díaz, M. Cases, M. Pastor, P. Marc, J. Wichard, K. Briggs, D. K. Watson, T. Kleinöder, C. Yang, A. Amberg, M. Beaumont, A. J. Brookes, S. Brunak, M. T. D. Cronin, G. F. Ecker, S. Escher, N. Greene, A. Guzmán, A. Hersey, P. Jacques, L. Lammens, J. Mestres, W. Muster, H. Northeved, M. Pinches, J. Saiz, N. Sajot, A. Valencia, J. van der Lei, N. P. E. Vermeulen, E. Vock, G. Wolber, and I. Zamora. Legacy data sharing to improve drug safety assessment: the eTOX project, Nat Rev Drug Discov, 16:811, 2017.
Links:
[doi:10.1038/nrd.2017.177]
[show BibTeX]
x
@article{RN250,
author = {Sanz, Ferran and Pognan, François and
Steger-Hartmann, Thomas and Díaz, Carlos
and Cases, Montserrat and Pastor, Manuel
and Marc, Philippe and Wichard, Joerg and
Briggs, Katharine and Watson, David K. and
Kleinöder, Thomas and Yang, Chihae and
Amberg, Alexander and Beaumont, Maria and
Brookes, Anthony J. and Brunak, Søren and
Cronin, Mark T. D. and Ecker, Gerhard F.
and Escher, Sylvia and Greene, Nigel and
Guzmán, Antonio and Hersey, Anne and
Jacques, Pascale and Lammens, Lieve and
Mestres, Jordi and Muster, Wolfgang and
Northeved, Helle and Pinches, Marc and
Saiz, Javier and Sajot, Nicolas and
Valencia, Alfonso and van der Lei, Johan
and Vermeulen, Nico P. E. and Vock, Esther
and Wolber, Gerhard and Zamora, Ismael},
title = {Legacy data sharing to improve drug
safety assessment: the eTOX project},
journal = {Nature Reviews Drug Discovery},
volume = {16},
pages = {811},
DOI = {10.1038/nrd.2017.177},
url = {http://dx.doi.org/10.1038/nrd.2017.177
http://www.nature.com/articles/nrd.2017.177.pdf},
year = {2017},
type = {Journal Article}
}
D. Schaller, S. Hagenow, G. Alpert, A. Naß, R. Schulz, M. Bermudez, H. Stark, and G. Wolber. Systematic data mining reveals synergistic H3R/MCHR1 ligands, ACS Medicinal Chemistry Letters, 8(6):648-653, 2017.
Links:
[doi:10.1021/acsmedchemlett.7b00118]
[show BibTeX]
[show abstract]
x
@article{RN202,
author = {Schaller, David and Hagenow, Stefanie and
Alpert, Gina and Naß, Alexandra and
Schulz, Robert and Bermudez, Marcel and
Stark, Holger and Wolber, Gerhard},
title = {Systematic data mining reveals
synergistic H3R/MCHR1 ligands},
journal = {ACS Medicinal Chemistry Letters},
volume = {8},
number = {6},
pages = {648-653},
abstract = {In this study, we report a ligand-centric
data mining approach that guided the
identification of suitable target profiles
for treating obesity. The newly developed
method is based on identifying target
pairs for synergistic positive effects and
also encompasses the exclusion of
compounds showing a detrimental effect on
obesity treatment (off-targets). Ligands
with known activity against
obesity-relevant targets were compared
using fingerprint representations. Similar
compounds with activities to different
targets were evaluated for the mechanism
of action since activation or deactivation
of drug targets determines the
pharmacological effect. In vitro
validation of the modeling results
revealed that three known modulators of
melanin-concentrating hormone receptor 1
(MCHR1) show a previously unknown
submicromolar affinity to the histamine H3
receptor (H3R). This synergistic activity
may present a novel therapeutic option
against obesity.},
keywords = {gpcr},
DOI = {10.1021/acsmedchemlett.7b00118},
url = {http://dx.doi.org/10.1021/acsmedchemlett.7b00118
http://pubs.acs.org/doi/pdfplus/10.1021/acsmedchemlett.7b00118},
year = {2017},
type = {Journal Article}
}
x
Systematic data mining reveals synergistic H3R/MCHR1 ligands
In this study, we report a ligand-centric data mining approach that guided the identification of suitable target profiles for treating obesity. The newly developed method is based on identifying target pairs for synergistic positive effects and also encompasses the exclusion of compounds showing a detrimental effect on obesity treatment (off-targets). Ligands with known activity against obesity-relevant targets were compared using fingerprint representations. Similar compounds with activities to different targets were evaluated for the mechanism of action since activation or deactivation of drug targets determines the pharmacological effect. In vitro validation of the modeling results revealed that three known modulators of melanin-concentrating hormone receptor 1 (MCHR1) show a previously unknown submicromolar affinity to the histamine H3 receptor (H3R). This synergistic activity may present a novel therapeutic option against obesity.
Q. Yan, D. Machalz, A. Zöllner, E. J. Sorensen, G. Wolber, and M. Bureik. Efficient substrate screening and inhibitor testing of human CYP4Z1 using permeabilized recombinant fission yeast, Biochem Pharmacol, 146:174-187, 2017.
Links:
[doi:10.1016/j.bcp.2017.09.011]
[show BibTeX]
[show abstract]
x
@article{RN206,
author = {Yan, Qi and Machalz, David and Zöllner,
Andy and Sorensen, Erik J. and Wolber,
Gerhard and Bureik, Matthias},
title = {Efficient substrate screening and
inhibitor testing of human CYP4Z1 using
permeabilized recombinant fission yeast},
journal = {Biochemical Pharmacology},
volume = {146},
pages = {174-187},
abstract = {We have established a protocol for the
preparation of permeabilized fission yeast
cells (enzyme bags) that recombinantly
express human cytochrome P450 enzymes
(CYPs). A direct comparison of CYP3A4
activity gave an eightfold higher
space-time yield for enzyme bag-catalyzed
biotransformation as compared to
whole-cell biotransformation, even though
the total number of cells employed was
lower by a factor of 150.
Biotransformation of the luminogenic
substrate Luciferin-H using
CYP2C9-containing enzyme bags proceeded
efficiently and stably for 24h. CYP4Z1 is
of interest because it is strongly
overexpressed both in breast cancer cells
and in breast cancer metastases; however,
current knowledge about its catalytic
properties is very limited. Screening of
CYP4Z1-containing enzyme bags with 15
luminogenic substrates enabled us to
identify two new hydroxylations and eleven
ether cleavage reactions that are
catalyzed by CYP4Z1. By far the best
substrate found in this study was
Luciferin benzyl ether (Luciferin-BE). On
the basis of the recently published
crystal structure of CYP4B1 we created a
new homology model of CYP4Z1 and performed
molecular docking experiments, which
indicate that all active substrates show a
highly similar binding geometry compared
to the endogenous substrates. The model
predicts that Ser113, Ser222, Asn381, and
Ser383 are key hydrogen bonding residues.
We also identified five new inhibitors of
CYP4Z1: miconazole, econazole,
aminobenzotriazole, tolazoline, and
1-benzylimidazole respectively, with the
last compound being the most potent giving
an IC50 value of 180nM in our test
system.},
keywords = {Human cytochrome P450 Biotransformation
Fission yeast Hydroxylation Ether
cleavage},
ISSN = {0006-2952},
DOI = {10.1016/j.bcp.2017.09.011},
url = {http://www.sciencedirect.com/science/article/pii/S000629521730607X},
year = {2017},
type = {Journal Article}
}
x
Efficient substrate screening and inhibitor testing of human CYP4Z1 using permeabilized recombinant fission yeast
We have established a protocol for the preparation of permeabilized fission yeast cells (enzyme bags) that recombinantly express human cytochrome P450 enzymes (CYPs). A direct comparison of CYP3A4 activity gave an eightfold higher space-time yield for enzyme bag-catalyzed biotransformation as compared to whole-cell biotransformation, even though the total number of cells employed was lower by a factor of 150. Biotransformation of the luminogenic substrate Luciferin-H using CYP2C9-containing enzyme bags proceeded efficiently and stably for 24h. CYP4Z1 is of interest because it is strongly overexpressed both in breast cancer cells and in breast cancer metastases; however, current knowledge about its catalytic properties is very limited. Screening of CYP4Z1-containing enzyme bags with 15 luminogenic substrates enabled us to identify two new hydroxylations and eleven ether cleavage reactions that are catalyzed by CYP4Z1. By far the best substrate found in this study was Luciferin benzyl ether (Luciferin-BE). On the basis of the recently published crystal structure of CYP4B1 we created a new homology model of CYP4Z1 and performed molecular docking experiments, which indicate that all active substrates show a highly similar binding geometry compared to the endogenous substrates. The model predicts that Ser113, Ser222, Asn381, and Ser383 are key hydrogen bonding residues. We also identified five new inhibitors of CYP4Z1: miconazole, econazole, aminobenzotriazole, tolazoline, and 1-benzylimidazole respectively, with the last compound being the most potent giving an IC50 value of 180nM in our test system.
J. Al-Asri, G. Gyémánt, E. Fazekas, G. Lehoczki, M. F. Melzig, G. Wolber, and J. Mortier. α-Amylase Modulation: Discovery of Inhibitors Using a Multi-Pharmacophore Approach for Virtual Screening, ChemMedChem, 11(21):2372-2377, 2016.
Links:
[doi:10.1002/cmdc.201600427]
[show BibTeX]
[show abstract]
x
@article{RN200,
author = {Al-Asri, Jamil and Gyémánt, Gyöngyi
and Fazekas, Erika and Lehoczki, Gábor
and Melzig, Matthias F. and Wolber,
Gerhard and Mortier, Jérémie},
title = {α-Amylase Modulation: Discovery of
Inhibitors Using a Multi-Pharmacophore
Approach for Virtual Screening},
journal = {ChemMedChem},
volume = {11},
number = {21},
pages = {2372-2377},
abstract = {Better control of postprandial
hyperglycemia can be achieved by delaying
the absorption of glucose resulting from
carbohydrate digestion. Because α-amylase
initiates the hydrolysis of
polysaccharides, the design of α-amylase
inhibitors can lead to the development of
new treatments for metabolic disorders
such as type II diabetes and obesity. In
this study, a rational computer-aided
approach was developed to identify novel
α-amylase inhibitors. Three-dimensional
pharmacophores were developed based on the
binding mode analysis of six different
families of compounds that bind to this
enzyme. In a stepwise virtual screening
workflow, seven molecules were selected
from a library of 1.4 million. Five out of
seven biologically tested compounds showed
α-amylase inhibition, and the two most
potent compounds inhibited α-amylase with
IC50 values of 17 and 27 μm. The
scaffold benzylideneacetohydrazide was
shared by four of the discovered
inhibitors, emerging as a novel drug-like
non-carbohydrate fragment and constituting
a promising lead scaffold for α-amylase
inhibition.},
keywords = {α-amylase computer-aided drug design
diabetes high-throughput virtual screening
lead discovery obesity},
ISSN = {1860-7187},
DOI = {10.1002/cmdc.201600427},
url = {http://dx.doi.org/10.1002/cmdc.201600427
http://onlinelibrary.wiley.com/store/10.1002/cmdc.201600427/asset/cmdc201600427.pdf?v= 1&t=iw6bvytr&s=b1ae9a27fb0f290b45c6a6997a8a3e9dff7b9678},
year = {2016},
type = {Journal Article}
}
x
α-Amylase Modulation: Discovery of Inhibitors Using a Multi-Pharmacophore Approach for Virtual Screening
Better control of postprandial hyperglycemia can be achieved by delaying the absorption of glucose resulting from carbohydrate digestion. Because α-amylase initiates the hydrolysis of polysaccharides, the design of α-amylase inhibitors can lead to the development of new treatments for metabolic disorders such as type II diabetes and obesity. In this study, a rational computer-aided approach was developed to identify novel α-amylase inhibitors. Three-dimensional pharmacophores were developed based on the binding mode analysis of six different families of compounds that bind to this enzyme. In a stepwise virtual screening workflow, seven molecules were selected from a library of 1.4 million. Five out of seven biologically tested compounds showed α-amylase inhibition, and the two most potent compounds inhibited α-amylase with IC50 values of 17 and 27 μm. The scaffold benzylideneacetohydrazide was shared by four of the discovered inhibitors, emerging as a novel drug-like non-carbohydrate fragment and constituting a promising lead scaffold for α-amylase inhibition.
D. Becker, Z. Kaczmarska, C. Arkona, R. Schulz, C. Tauber, G. Wolber, R. Hilgenfeld, M. Coll, and J. Rademann. Irreversible inhibitors of the 3C protease of Coxsackie virus through templated assembly of protein-binding fragments, Nat Commun, 7:12761, 2016.
Links:
[doi:10.1038/ncomms12761]
[show BibTeX]
x
@article{RN198,
author = {Becker, Daniel and Kaczmarska, Zuzanna
and Arkona, Christoph and Schulz, Robert
and Tauber, Carolin and Wolber, Gerhard
and Hilgenfeld, Rolf and Coll, Miquel and
Rademann, Jörg},
title = {Irreversible inhibitors of the 3C
protease of Coxsackie virus through
templated assembly of protein-binding
fragments},
journal = {Nature Communications},
volume = {7},
pages = {12761},
DOI = {10.1038/ncomms12761},
url = {http://dx.doi.org/10.1038/ncomms12761
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5052702/pdf/ncomms12761.pdf},
year = {2016},
type = {Journal Article}
}
M. Bermudez, J. Mortier, C. Rakers, D. Sydow, and G. Wolber. More than a look into a crystal ball: protein structure elucidation guided by molecular dynamics simulations, Drug Discov Today, 21(11):1799-1805, 2016.
Links:
[doi:10.1016/j.drudis.2016.07.001]
[show BibTeX]
[show abstract]
x
@article{RN193,
author = {Bermudez, Marcel and Mortier, Jeremie and
Rakers, Christin and Sydow, Dominique and
Wolber, Gerhard},
title = {More than a look into a crystal ball:
protein structure elucidation guided by
molecular dynamics simulations},
journal = {Drug Discovery Today},
volume = {21},
number = {11},
pages = {1799-1805},
abstract = {The ‘form follows function’ principle
implies that a structural determination of
protein structures is indispensable to
understand proteins in their biological
roles. However, experimental methods still
show shortcomings in the description of
the dynamic properties of proteins.
Therefore, molecular dynamics (MD)
simulations represent an essential tool
for structural biology to investigate
proteins as flexible and dynamic entities.
Here, we will give an overview on the
impact of MD simulations on structural
investigations, including studies that aim
at a prediction of protein-folding
pathways, protein-assembly processes and
the sampling of conformational space by
computational means.},
ISSN = {1359-6446},
DOI = {10.1016/j.drudis.2016.07.001},
url = {http://www.sciencedirect.com/science/article/pii/S1359644616302513
https://pdf.sciencedirectassets.com/271275/1-s2.0-S1359644616X00128/1-s2.0-S1359644616302513/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJHMEUCIQDpMFAxqO8i5cWwO8J2bidQOOPWWOgX2xYgjlCqnmYpKAIgMz6zEqJOOqjcysWG%2F%2BjDZIPEG4hPysl42TQ%2BxrkJgLgq2gMIQRACGgwwNTkwMDM1NDY4NjUiDMbVMeQJp%2BA6xuTeeiq3A5yGUmiSxbecX6zyK9C0u9d8FvveGrkPLG2bgf%2FLpoQrYo2WyvA%2BBWqUubZ%2F%2F2lzFcSTpeDFbT3qjC0N3gXJAnlrhfnB4HtdM5JkFOs7rk4T5uxWa2QB%2Bxrb0uGWp6C%2FKniuq6eBMiJ59EW8Qua9SC1HjapOsfuus9g5HoQTBdya%2B%2B93vPUyT1l6n3DvNF79aTePLdf4GzCoc3La3CqQwXZ%2Bv00XVVkSLXyHqEtn%2BKj4RqGYDx5vybmCYgNtuQnt%2F4u%2FKij1yfRzcl%2BKOheRiB2UBQBFkx%2BGRL%2BqBFLx23kYZU2PrVD4ZjQMMXyglfn7R41W1Ts944jcTs7eKtPEaIuwbGZFi3rfxU%2BBL%2BrhXsqDJMbPTE%2BrqYpk6xiwMVJaY6kJH62kCLazVZrQ49cjhJjxeJti4aqVRxjr4XOP%2FJ9EatP%2BZoD3q47yNzrS1dG3FcQOp6Rtx1LGD9QPU8GrLpoB2%2F%2BrydWuCVqCFWw5iNbyp05sWQsfmSpFEMzsvkKO%2FCw0bATpkHdkF6WOn0A1lXIOsI0cDUPAe1xWJ3L0KZySQ0UZUqXJ8Gw4v%2FTlL5BY%2FUxSnor26xYwn6aO6wU6tAHBqopuW5pcz%2FJI%2BrCyUdYGVL8ZGUcN9osxZNHlsdHl%2FPSb5Ey3HqdiHTks1pszrFK8F0ZxsQNCYajkgKT3SAzhgrTmaNYfXyzLVkl3rMbW5QA0SqgsnR79JfHgUXuHGBnWyrsVF5mu9vmFjpTB4j2VqmtMOIjghMdHO%2FOIcR59ey2VTJQKE1Ulq0WJqECO72nl8nHMFEUoBLcWlCu5cwJlo5NxAcL7qC1hNPBzJlNFYMqU07s%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090752Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY5J43SP76%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=41d5423206e9b4da9d8bad5f7023e4f96b18b2664c923fbc9de439a7f34293c4&hash=218a0f350491d1c5c383f9505b728b13f7879d7491bb1fb18a042fb95213e569&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S1359644616302513&tid=spdf-4c8cdfd3-7d8d-4705-a232-8bf4948ce6eb&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2016},
type = {Journal Article}
}
x
More than a look into a crystal ball: protein structure elucidation guided by molecular dynamics simulations
The ‘form follows function’ principle implies that a structural determination of protein structures is indispensable to understand proteins in their biological roles. However, experimental methods still show shortcomings in the description of the dynamic properties of proteins. Therefore, molecular dynamics (MD) simulations represent an essential tool for structural biology to investigate proteins as flexible and dynamic entities. Here, we will give an overview on the impact of MD simulations on structural investigations, including studies that aim at a prediction of protein-folding pathways, protein-assembly processes and the sampling of conformational space by computational means.
A. Bock, M. Bermudez, F. Krebs, C. Matera, B. Chirinda, D. Sydow, C. Dallanoce, U. Holzgrabe, M. De Amici, M. J. Lohse, G. Wolber, and K. Mohr. Ligand Binding Ensembles Determine Graded Agonist Efficacies at a G Protein-Coupled Receptor, J Biol Chem, 291(31):16375-16389, 2016.
Links:
[doi:10.1074/jbc.M116.735431]
[show BibTeX]
[show abstract]
x
@article{RN197,
author = {Bock, Andreas and Bermudez, Marcel and
Krebs, Fabian and Matera, Carlo and
Chirinda, Brian and Sydow, Dominique and
Dallanoce, Clelia and Holzgrabe, Ulrike
and De Amici, Marco and Lohse, Martin J.
and Wolber, Gerhard and Mohr, Klaus},
title = {Ligand Binding Ensembles Determine Graded
Agonist Efficacies at a G Protein-Coupled
Receptor},
journal = {Journal of Biological Chemistry},
volume = {291},
number = {31},
pages = {16375-16389},
abstract = {G protein-coupled receptors constitute
the largest family of membrane receptors
and modulate almost every physiological
process in humans. Binding of agonists to
G protein-coupled receptors induces a
shift from inactive to active receptor
conformations. Biophysical studies of the
dynamic equilibrium of receptors suggest
that a portion of receptors can remain in
inactive states even in the presence of
saturating concentrations of agonist and G
protein mimetic. However, the molecular
details of agonist-bound inactive
receptors are poorly understood. Here we
use the model of bitopic
orthosteric/allosteric (i.e. dualsteric)
agonists for muscarinic M2 receptors to
demonstrate the existence and function of
such inactive agonist·receptor complexes
on a molecular level. Using all-atom
molecular dynamics simulations, dynophores
(i.e. a combination of static
three-dimensional pharmacophores and
molecular dynamics-based conformational
sampling), ligand design, and receptor
mutagenesis, we show that inactive
agonist·receptor complexes can result
from agonist binding to the allosteric
vestibule alone, whereas the dualsteric
binding mode produces active receptors.
Each agonist forms a distinct ligand
binding ensemble, and different agonist
efficacies depend on the fraction of
purely allosteric (i.e. inactive) versus
dualsteric (i.e. active) binding modes. We
propose that this concept may explain why
agonist·receptor complexes can be
inactive and that adopting multiple
binding modes may be generalized also to
small agonists where binding modes will be
only subtly different and confined to only
one binding site.},
keywords = {gpcr},
DOI = {10.1074/jbc.M116.735431},
url = {http://www.jbc.org/content/291/31/16375.abstract
http://www.jbc.org/content/291/31/16375.full.pdf},
year = {2016},
type = {Journal Article}
}
x
Ligand Binding Ensembles Determine Graded Agonist Efficacies at a G Protein-Coupled Receptor
G protein-coupled receptors constitute the largest family of membrane receptors and modulate almost every physiological process in humans. Binding of agonists to G protein-coupled receptors induces a shift from inactive to active receptor conformations. Biophysical studies of the dynamic equilibrium of receptors suggest that a portion of receptors can remain in inactive states even in the presence of saturating concentrations of agonist and G protein mimetic. However, the molecular details of agonist-bound inactive receptors are poorly understood. Here we use the model of bitopic orthosteric/allosteric (i.e. dualsteric) agonists for muscarinic M2 receptors to demonstrate the existence and function of such inactive agonist·receptor complexes on a molecular level. Using all-atom molecular dynamics simulations, dynophores (i.e. a combination of static three-dimensional pharmacophores and molecular dynamics-based conformational sampling), ligand design, and receptor mutagenesis, we show that inactive agonist·receptor complexes can result from agonist binding to the allosteric vestibule alone, whereas the dualsteric binding mode produces active receptors. Each agonist forms a distinct ligand binding ensemble, and different agonist efficacies depend on the fraction of purely allosteric (i.e. inactive) versus dualsteric (i.e. active) binding modes. We propose that this concept may explain why agonist·receptor complexes can be inactive and that adopting multiple binding modes may be generalized also to small agonists where binding modes will be only subtly different and confined to only one binding site.
S. Bock, M. S. Murgueitio, G. Wolber, and G. Weindl. Acute myeloid leukaemia-derived Langerhans-like cells enhance Th1 polarization upon TLR2 engagement, Pharmacol Res, 105:44-53, 2016.
Links:
[doi:10.1016/j.phrs.2016.01.016]
[show BibTeX]
[show abstract]
x
@article{RN183,
author = {Bock, Stephanie and Murgueitio, Manuela
S. and Wolber, Gerhard and Weindl,
Günther},
title = {Acute myeloid leukaemia-derived
Langerhans-like cells enhance Th1
polarization upon TLR2 engagement},
journal = {Pharmacological Research},
volume = {105},
pages = {44-53},
abstract = {Langerhans cells (LCs) represent a highly
specialized subset of epidermal dendritic
cells (DCs), yet not fully understood in
their function of balancing skin immunity.
Here, we investigated in vitro generated
Langerhans-like cells obtained from the
human acute myeloid leukaemia cell line
MUTZ-3 (MUTZ-LCs) to study TLR- and
cytokine-dependent activation of epidermal
DCs. MUTZ-LCs revealed high TLR2
expression and responded robustly to TLR2
engagement, confirmed by increased CD83,
CD86, PD-L1 and IDO expression,
upregulated IL-6, IL-12p40 and IL-23p19
mRNA levels IL-8 release. TLR2 activation
reduced CCR6 and elevated CCR7 mRNA
expression and induced migration of
MUTZ-LCs towards CCL21. Similar results
were obtained by stimulation with
pro-inflammatory cytokines TNF-α and
IL-1β whereas ligands of TLR3 and TLR4
failed to induce a fully mature phenotype.
Despite limited cytokine gene expression
and production for TLR2-activated
MUTZ-LCs, co-culture with naive CD4+ T
cells led to significantly increased
IFN-γ and IL-22 levels indicating Th1
differentiation independent of IL-12.
TLR2-mediated effects were blocked by the
putative TLR2/1 antagonist CU-CPT22,
however, no selectivity for either TLR2/1
or TLR2/6 was observed. Computer-aided
docking studies confirmed non-selective
binding of the TLR2 antagonist. Taken
together, our results indicate a critical
role for TLR2 signalling in MUTZ-LCs
considering the leukemic origin of the
generated Langerhans-like cells.},
keywords = {Acute myeloid leukaemia (AML) Langerhans
cells Toll-like receptors (TLR) TLR2
Pro-inflammatory cytokines T helper type 1
(Th1) cells},
ISSN = {1043-6618},
DOI = {10.1016/j.phrs.2016.01.016},
url = {http://www.sciencedirect.com/science/article/pii/S1043661816000220},
year = {2016},
type = {Journal Article}
}
x
Acute myeloid leukaemia-derived Langerhans-like cells enhance Th1 polarization upon TLR2 engagement
Langerhans cells (LCs) represent a highly specialized subset of epidermal dendritic cells (DCs), yet not fully understood in their function of balancing skin immunity. Here, we investigated in vitro generated Langerhans-like cells obtained from the human acute myeloid leukaemia cell line MUTZ-3 (MUTZ-LCs) to study TLR- and cytokine-dependent activation of epidermal DCs. MUTZ-LCs revealed high TLR2 expression and responded robustly to TLR2 engagement, confirmed by increased CD83, CD86, PD-L1 and IDO expression, upregulated IL-6, IL-12p40 and IL-23p19 mRNA levels IL-8 release. TLR2 activation reduced CCR6 and elevated CCR7 mRNA expression and induced migration of MUTZ-LCs towards CCL21. Similar results were obtained by stimulation with pro-inflammatory cytokines TNF-α and IL-1β whereas ligands of TLR3 and TLR4 failed to induce a fully mature phenotype. Despite limited cytokine gene expression and production for TLR2-activated MUTZ-LCs, co-culture with naive CD4+ T cells led to significantly increased IFN-γ and IL-22 levels indicating Th1 differentiation independent of IL-12. TLR2-mediated effects were blocked by the putative TLR2/1 antagonist CU-CPT22, however, no selectivity for either TLR2/1 or TLR2/6 was observed. Computer-aided docking studies confirmed non-selective binding of the TLR2 antagonist. Taken together, our results indicate a critical role for TLR2 signalling in MUTZ-LCs considering the leukemic origin of the generated Langerhans-like cells.
E. Guerrieri, M. Bermudez, G. Wolber, I. P. Berzetei-Gurske, H. Schmidhammer, and M. Spetea. Structural determinants of diphenethylamines for interaction with the κ opioid receptor: Synthesis, pharmacology and molecular modeling studies, Bioorg Med Chem Lett, 26(19):4769-4774, 2016.
Links:
[doi:10.1016/j.bmcl.2016.08.031]
[show BibTeX]
[show abstract]
x
@article{RN191,
author = {Guerrieri, Elena and Bermudez, Marcel and
Wolber, Gerhard and Berzetei-Gurske, Ilona
P. and Schmidhammer, Helmut and Spetea,
Mariana},
title = {Structural determinants of
diphenethylamines for interaction with the
κ opioid receptor: Synthesis,
pharmacology and molecular modeling
studies},
journal = {Bioorganic & Medicinal Chemistry
Letters},
volume = {26},
number = {19},
pages = {4769-4774},
abstract = {The κ opioid (KOP) receptor crystal
structure in an inactive state offers
nowadays a valuable platform for inquiry
into receptor function. We describe the
synthesis, pharmacological evaluation and
docking calculations of KOP receptor
ligands from the class of
diphenethylamines using an active-like
structure of the KOP receptor attained by
molecular dynamics simulations. The
structure–activity relationships derived
from computational studies was in
accordance with pharmacological activities
of targeted diphenethylamines at the KOP
receptor established by competition
binding and G protein activation in vitro
assays. Our analysis identified that
agonist binding results in breaking of the
Arg156-Thr273 hydrogen bond, which
stabilizes the inactive receptor
conformation, and a crucial hydrogen bond
with His291 is formed. Compounds with a
phenolic 4-hydroxy group do not form the
hydrogen bond with His291, an important
residue for KOP affinity and agonist
activity. The size of the N-substituent
hosted by the hydrophobic pocket formed by
Val108, Ile316 and Tyr320 considerably
influences binding and selectivity, with
the n-alkyl size limit being five carbon
atoms, while bulky substituents turn KOP
agonists in antagonists. Thus, combination
of experimental and molecular modeling
strategies provides an initial framework
for understanding the structural features
of diphenethylamines that are essential to
promote binding affinity and selectivity
for the KOP receptor, and may be involved
in transduction of the ligand binding
event into molecular changes, ultimately
leading to receptor activation.},
keywords = {κ opioid receptor Diphenethylamine
Agonist Antagonist Partial agonist SAR
Molecular dynamics gpcr},
ISSN = {0960-894X},
DOI = {10.1016/j.bmcl.2016.08.031},
url = {http://www.sciencedirect.com/science/article/pii/S0960894X16308526
https://pdf.sciencedirectassets.com/271398/1-s2.0-S0960894X16X00182/1-s2.0-S0960894X16308526/main.pdf?X-Amz-Security-Token= AgoJb3JpZ2luX2VjEJn%2F%2F%2F%2F%2F%2F%2F%2F%2F%2FwEaCXVzLWVhc3QtMSJGMEQCIBSuXR284U5c9bmAJSN5zVw47n5M5VTNwrBmGQ8hLXVcAiAWIgoCDAYq%2BrLMnoeOj0gcwMsJ%2BK2%2F2vx37jlwfG9V%2FiraAwhBEAIaDDA1OTAwMzU0Njg2NSIMjEBFMn9jIE9t1XN2KrcDLe7GRG%2FXcw3WEgKV9YXbSGYcHYB2z7r%2B9eRerOT0zpE0wRSFgQ%2FOH9BXlp37MIlzoDaMcVajFKZS8gcJXSICZZRdMHztkVCBW4DNZa%2BJxOwJ1E6Q6vzyyBGsc%2FRoV6Xba79A94ndoN8bYy6WNuplWyrl3mWsK2vv7sI6kqAQYvwZAuSo66P%2FLefOdgACsLXUvDUtMMpSN8TmoE6MAFh%2Fj9u1tN2PHd7Si9U0lR%2BPybWjyQIZhRLaPFiPA459dq%2F0DJYDW6PUXjnBlCqj2pjkDd8G%2FcrPGVCvjIs1dEukqNYRPh9wSQFFAb5Fi0wZV3zG%2F46Hc%2FeUQIprBOrsYtxFvQ1UWxSkaVfuuRD2NfJJC%2FoyWTyuxNmD0bdhukh9A21%2B1W83wJfu%2FecDVcxhN9MqXTDLroqrvl%2BRxWFnb%2BWbs%2B%2FV9bgL2cBI6AFt4X60UAEM%2FPSuYh%2FCMbaK1dtKiQLD4N%2F7kxQrpF%2BWzb6z4YOF5hW%2F4Jm%2BgH%2B0v9v9lego%2FcMGf98VdcRdShnMV0057mzmxPdKDL4AivAyKRRfnHrHbLoO1SdPlc41DKXR%2BjK0GtocjZ5j4aUAxDDnpo7rBTq1AThXMgqWLXhCysjiDb69KUDsmtirRuTrXpezLGPDfxkFp5ofIqWUR9J0yQNBLS%2BqlghqmfiGup4DJm90ED%2Bjr269Tuc2Ri4J0zfr7%2BcI1xixL%2Fy0rAQI30TJNbf1CsDGxfVixVDBG1Fkwa3KlpjjfAGAuGKaiPkPOMJuLo8YTbs28PVbbZSstkpcNX0npq661wJ6SM3c0BNDLzt2HCCzsKCoWT8MzDtnFnmY8Er2Z7ayg87lxx0%3D&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20190826T090730Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTYZNRZYJFV%2F20190826%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=037fa3ee2555131e64b8b5688e7e4a686e30cbef207e6458fb55f96764b245fb&hash=08167983ee3f84a7da97f9261f1c7a8c6d2025de5b3aa82b896df1eb7cd149b2&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S0960894X16308526&tid=spdf-8f3d8366-6e13-4767-a6f4-96592d64c57f&sid=2685c81b50af534a6f99d1c429305bd4118dgxrqb&type=client},
year = {2016},
type = {Journal Article}
}
x
Structural determinants of diphenethylamines for interaction with the κ opioid receptor: Synthesis, pharmacology and molecular modeling studies
The κ opioid (KOP) receptor crystal structure in an inactive state offers nowadays a valuable platform for inquiry into receptor function. We describe the synthesis, pharmacological evaluation and docking calculations of KOP receptor ligands from the class of diphenethylamines using an active-like structure of the KOP receptor attained by molecular dynamics simulations. The structure–activity relationships derived from computational studies was in accordance with pharmacological activities of targeted diphenethylamines at the KOP receptor established by competition binding and G protein activation in vitro assays. Our analysis identified that agonist binding results in breaking of the Arg156-Thr273 hydrogen bond, which stabilizes the inactive receptor conformation, and a crucial hydrogen bond with His291 is formed. Compounds with a phenolic 4-hydroxy group do not form the hydrogen bond with His291, an important residue for KOP affinity and agonist activity. The size of the N-substituent hosted by the hydrophobic pocket formed by Val108, Ile316 and Tyr320 considerably influences binding and selectivity, with the n-alkyl size limit being five carbon atoms, while bulky substituents turn KOP agonists in antagonists. Thus, combination of experimental and molecular modeling strategies provides an initial framework for understanding the structural features of diphenethylamines that are essential to promote binding affinity and selectivity for the KOP receptor, and may be involved in transduction of the ligand binding event into molecular changes, ultimately leading to receptor activation.
J. R. Homoki, A. Nemes, E. Fazekas, G. Gyémánt, P. Balogh, F. Gál, J. Al-Asri, J. Mortier, G. Wolber, L. Babinszky, and J. Remenyik. Anthocyanin composition, antioxidant efficiency, and α-amylase inhibitor activity of different Hungarian sour cherry varieties (Prunus cerasus L.), Food Chem, 194:222-229, 2016.
Links:
[doi:10.1016/j.foodchem.2015.07.130]
[show BibTeX]
[show abstract]
x
@article{RN177,
author = {Homoki, Judit R. and Nemes, Andrea and
Fazekas, Erika and Gyémánt, Gyöngyi and
Balogh, Péter and Gál, Ferenc and
Al-Asri, Jamil and Mortier, Jérémie and
Wolber, Gerhard and Babinszky, László
and Remenyik, Judit},
title = {Anthocyanin composition, antioxidant
efficiency, and α-amylase inhibitor
activity of different Hungarian sour
cherry varieties (Prunus cerasus L.)},
journal = {Food Chemistry},
volume = {194},
pages = {222-229},
abstract = {Five Hungarian sour cherry cultivars were
studied to determine their anthocyanin
contents and their possible inhibitory
properties. The water and methanol soluble
antioxidant capacities were separately
assessed by photoluminescence showing
values ranged from 3.4 μg mg−1 to 15.4
μg mg−1, respectively. The “VN1”
variety (selected from “Csengődi
csokros”) showed the highest antioxidant
capacity. The anthocyanin content,
measured by pH differential method or
isolated by solid phase extraction, was
the highest also in “VN1”. Correlation
was found between the anthocyanin content
and the high antioxidant capacity. The
main anthocyanin components were
cyanidin-3-O-rutinoside and
cyanidin-3-O-glucoside. The presence of
malvidin-3,5-O-diglycoside was verified by
MALDI-TOF MS. Sour cherry extracts and
selected anthocyanins inhibited the human
salivary alpha-amylase catalyzed
hydrolysis competitively. The lowest IC50
value, 55 μg mL−1 or 80 μM, was
measured for malvidin-3,5-O-diglycoside,
for which possible binding modes within
the alpha-amylase active site could be
investigated in silico using molecular
docking and molecular dynamics.},
keywords = {Sour cherry Antioxidants Anthocyanins
Human salivary α-amylase Inhibition},
ISSN = {0308-8146},
DOI = {10.1016/j.foodchem.2015.07.130},
url = {http://www.sciencedirect.com/science/article/pii/S0308814615011644
http://ac.els-cdn.com/S0308814615011644/1-s2.0-S0308814615011644-main.pdf?_tid= 626cf250-a00c-11e5-9e98-00000aacb35d&acdnat=1449841255_9edc44dca9e7c73085280f5fd716d846},
year = {2016},
type = {Journal Article}
}
x
Anthocyanin composition, antioxidant efficiency, and α-amylase inhibitor activity of different Hungarian sour cherry varieties (Prunus cerasus L.)
Five Hungarian sour cherry cultivars were studied to determine their anthocyanin contents and their possible inhibitory properties. The water and methanol soluble antioxidant capacities were separately assessed by photoluminescence showing values ranged from 3.4 μg mg−1 to 15.4 μg mg−1, respectively. The “VN1” variety (selected from “Csengődi csokros”) showed the highest antioxidant capacity. The anthocyanin content, measured by pH differential method or isolated by solid phase extraction, was the highest also in “VN1”. Correlation was found between the anthocyanin content and the high antioxidant capacity. The main anthocyanin components were cyanidin-3-O-rutinoside and cyanidin-3-O-glucoside. The presence of malvidin-3,5-O-diglycoside was verified by MALDI-TOF MS. Sour cherry extracts and selected anthocyanins inhibited the human salivary alpha-amylase catalyzed hydrolysis competitively. The lowest IC50 value, 55 μg mL−1 or 80 μM, was measured for malvidin-3,5-O-diglycoside, for which possible binding modes within the alpha-amylase active site could be investigated in silico using molecular docking and molecular dynamics.
S. Köhling, G. Künze, K. Lemmnitzer, M. Bermudez, G. Wolber, J. Schiller, D. Huster, and J. Rademann. Chemoenzymatic synthesis of nonasulfated tetrahyaluronan with a paramagnetic tag for studying its complex with Interleukin-10, Chemistry - A European Journal, 22(16): 5563-5574, 2016.
Links:
[doi:10.1002/chem.201504459]
[show BibTeX]
[show abstract]
x
@article{RN184,
author = {Köhling, Sebastian and Künze, Georg and
Lemmnitzer, Katharina and Bermudez, Marcel
and Wolber, Gerhard and Schiller, Jürgen
and Huster, Daniel and Rademann, Jörg},
title = {Chemoenzymatic synthesis of nonasulfated
tetrahyaluronan with a paramagnetic tag
for studying its complex with
Interleukin-10},
journal = {Chemistry - A European Journal},
volume = {22},
number = {16},
pages = { 5563-5574},
abstract = {Implants and artificial biomaterials
containing sulfated hyaluronans have been
shown to improve the healing of injured
skin and bones. It is hypothesized that
these effects are mediated by the binding
of sulfated glycosaminoglycans (GAGs) to
growth factors and cytokines, resulting in
the sequestering of proteins to the wound
healing site and in modulated protein
activity. Given that no direct synthetic
access to sulfated oligohyaluronans has
been available, little is known about
their protein binding and the structure of
the resulting protein complexes. Here, the
chemoenzymatic preparation of
oligohyaluronans on the gram scale is
described. Oligohyaluronans are converted
into anomeric azides at the reducing end,
enabling the attachment of analytical
labels through an anomeric ligation
reaction. A nonasulfated
tetrahyaluronan–ethylenediaminetetraacetic
acid derivative has been produced and used
as a paramagnetic tag for the elucidation
of the complex of this ligand with
interleukin-10 using paramagnetic
relaxation enhancement NMR analysis. The
metal ion position is resolved with
1.0 Å, enabling a refined structural
model of the complex.},
keywords = {carbohydrates cytokines growth factors
host–guest systems protein structures},
ISSN = {1521-3765},
DOI = {10.1002/chem.201504459},
url = {http://dx.doi.org/10.1002/chem.201504459
http://onlinelibrary.wiley.com/store/10.1002/chem.201504459/asset/chem201504459.pdf?v= 1&t=ili6eox1&s=58b1d54b76bcf28cf8559dcb069e2abdebc61887},
year = {2016},
type = {Journal Article}
}
x
Chemoenzymatic synthesis of nonasulfated tetrahyaluronan with a paramagnetic tag for studying its complex with Interleukin-10
Implants and artificial biomaterials containing sulfated hyaluronans have been shown to improve the healing of injured skin and bones. It is hypothesized that these effects are mediated by the binding of sulfated glycosaminoglycans (GAGs) to growth factors and cytokines, resulting in the sequestering of proteins to the wound healing site and in modulated protein activity. Given that no direct synthetic access to sulfated oligohyaluronans has been available, little is known about their protein binding and the structure of the resulting protein complexes. Here, the chemoenzymatic preparation of oligohyaluronans on the gram scale is described. Oligohyaluronans are converted into anomeric azides at the reducing end, enabling the attachment of analytical labels through an anomeric ligation reaction. A nonasulfated tetrahyaluronan–ethylenediaminetetraacetic acid derivative has been produced and used as a paramagnetic tag for the elucidation of the complex of this ligand with interleukin-10 using paramagnetic relaxation enhancement NMR analysis. The metal ion position is resolved with 1.0 Å, enabling a refined structural model of the complex.
B. Nizami, D. Sydow, G. Wolber, and B. Honarparvar. Molecular insight on the binding of NNRTI to K103N mutated HIV-1 RT: molecular dynamics simulations and dynamic pharmacophore analysis, Mol Biosyst, 12:3385-3395, 2016.
Links:
[doi:10.1039/C6MB00428H]
[show BibTeX]
x
@article{RN196,
author = {Nizami, Bilal and Sydow, Dominique and
Wolber, Gerhard and Honarparvar, Bahareh},
title = {Molecular insight on the binding of NNRTI
to K103N mutated HIV-1 RT: molecular
dynamics simulations and dynamic
pharmacophore analysis},
journal = {Molecular BioSystems},
volume = {12},
pages = {3385-3395},
DOI = {10.1039/C6MB00428H},
url = {https://pubs.rsc.org/en/content/articlepdf/2016/mb/c6mb00428h},
year = {2016},
type = {Journal Article}
}
V. Obermoser, M. E. Urban, M. S. Murgueitio, G. Wolber, U. Kintscher, and R. Gust. New telmisartan-derived PPARγ agonists: Impact of the 3D-binding mode on the pharmacological profile, Eur J Med Chem, 124:138-152, 2016.
Links:
[doi:10.1016/j.ejmech.2016.08.027]
[show BibTeX]
[show abstract]
x
@article{RN194,
author = {Obermoser, Victoria and Urban, Margarethe
E. and Murgueitio, Manuela S. and Wolber,
Gerhard and Kintscher, Ulrich and Gust,
Ronald},
title = {New telmisartan-derived PPARγ agonists:
Impact of the 3D-binding mode on the
pharmacological profile},
journal = {European Journal of Medicinal Chemistry},
volume = {124},
pages = {138-152},
abstract = {In previous studies, the
4′-((2-propyl-1H-benzo[d]imidazol-1-yl)methyl)-[1,1′-biphenyl]-2-carboxylic
acid was identified as pharmacophoric core
for PPARγ activation. In this
structure-activity relationship study the
C2-alkyl chain was elongated and the
2-COOH group was changed to a
carbamide/nitrile or shifted to the 3- or
4-position. Furthermore, the
benzo[d]imidazole was exchanged by
2,3-dihydrobenzo[d]thiazole or 1H-indole.
C2-propyl derivatives showed the profile
of partial agonists, while elongation of
the C2-chain to that of an n-heptyl group
or a 4-COOH shift changed the
pharmacological profile to that of a
potent full agonist. This finding can be
explained by binding to the LBD in
different ligand conformations. Two
anchoring points (Tyr473 and Arg288) exist
in the LBD, which have to be contacted to
achieve receptor activation. In a crystal
violet chemosensitivity assay using
COS-7 cells and LNCaP cells expressing
PPARγ only the carboxamide derivatives
influenced the cell growth, independently
on the presence of the PPARγ. Therefore,
receptor mediated cytotoxicity can be
excluded.},
keywords = {PPARγ Transactivation Cytotoxicity
Binding mode Molecular modeling},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2016.08.027},
url = {http://www.sciencedirect.com/science/article/pii/S0223523416306705
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year = {2016},
type = {Journal Article}
}
x
New telmisartan-derived PPARγ agonists: Impact of the 3D-binding mode on the pharmacological profile
In previous studies, the 4′-((2-propyl-1H-benzo[d]imidazol-1-yl)methyl)-[1,1′-biphenyl]-2-carboxylic acid was identified as pharmacophoric core for PPARγ activation. In this structure-activity relationship study the C2-alkyl chain was elongated and the 2-COOH group was changed to a carbamide/nitrile or shifted to the 3- or 4-position. Furthermore, the benzo[d]imidazole was exchanged by 2,3-dihydrobenzo[d]thiazole or 1H-indole. C2-propyl derivatives showed the profile of partial agonists, while elongation of the C2-chain to that of an n-heptyl group or a 4-COOH shift changed the pharmacological profile to that of a potent full agonist. This finding can be explained by binding to the LBD in different ligand conformations. Two anchoring points (Tyr473 and Arg288) exist in the LBD, which have to be contacted to achieve receptor activation. In a crystal violet chemosensitivity assay using COS-7 cells and LNCaP cells expressing PPARγ only the carboxamide derivatives influenced the cell growth, independently on the presence of the PPARγ. Therefore, receptor mediated cytotoxicity can be excluded.
C. Rakers, F. Schumacher, W. Meinl, H. Glatt, B. Kleuser, and G. Wolber. In silico prediction of human sulfotransferase 1E1 activity guided by pharmacophores from molecular dynamics simulations, J Biol Chem, 291(1):58-71, 2016.
Links:
[doi:10.1074/jbc.M115.685610]
[show BibTeX]
[show abstract]
x
@article{RN180,
author = {Rakers, Christin and Schumacher, Fabian
and Meinl, Walter and Glatt, Hansruedi and
Kleuser, Burkhard and Wolber, Gerhard},
title = {In silico prediction of human
sulfotransferase 1E1 activity guided by
pharmacophores from molecular dynamics
simulations},
journal = {Journal of Biological Chemistry},
volume = {291},
number = {1},
pages = {58-71},
abstract = {Acting during phase II metabolism,
sulfotransferases (SULTs) serve
detoxification by transforming a broad
spectrum of compounds from pharmaceutical,
nutritional, or environmental sources into
more easily excretable metabolites.
However, SULT activity has also been shown
to promote formation of reactive
metabolites that may have genotoxic
effects. SULT subtype 1E1 (SULT1E1) was
identified as a key player in estrogen
homeostasis, which is involved in many
physiological processes and the
pathogenesis of breast and endometrial
cancer. The development of an in silico
prediction model for SULT1E1 ligands would
therefore support the development of
metabolically inert drugs and help to
assess health risks related to hormonal
imbalances. Here, we report on a novel
approach to develop a model that enables
prediction of substrates and inhibitors of
SULT1E1. Molecular dynamics simulations
were performed to investigate enzyme
flexibility and sample protein
conformations. Pharmacophores were
developed that served as a cornerstone of
the model and machine learning techniques
were applied for prediction refinement.
The prediction model was used to screen
the DrugBank (a database of experimental
and approved drugs): 28 % of the predicted
hits were reported in literature as
ligands of SULT1E1. From the remaining
hits, a selection of nine molecules was
subjected to biochemical assay validation
and experimental results were in
accordance with the in silico prediction
of SULT1E1 inhibitors and substrates, thus
affirming our prediction hypotheses.},
DOI = {10.1074/jbc.M115.685610},
url = {http://www.jbc.org/content/early/2015/11/05/jbc.M115.685610.abstract
http://www.jbc.org/content/early/2015/11/05/jbc.M115.685610.full.pdf},
year = {2016},
type = {Journal Article}
}
x
In silico prediction of human sulfotransferase 1E1 activity guided by pharmacophores from molecular dynamics simulations
Acting during phase II metabolism, sulfotransferases (SULTs) serve detoxification by transforming a broad spectrum of compounds from pharmaceutical, nutritional, or environmental sources into more easily excretable metabolites. However, SULT activity has also been shown to promote formation of reactive metabolites that may have genotoxic effects. SULT subtype 1E1 (SULT1E1) was identified as a key player in estrogen homeostasis, which is involved in many physiological processes and the pathogenesis of breast and endometrial cancer. The development of an in silico prediction model for SULT1E1 ligands would therefore support the development of metabolically inert drugs and help to assess health risks related to hormonal imbalances. Here, we report on a novel approach to develop a model that enables prediction of substrates and inhibitors of SULT1E1. Molecular dynamics simulations were performed to investigate enzyme flexibility and sample protein conformations. Pharmacophores were developed that served as a cornerstone of the model and machine learning techniques were applied for prediction refinement. The prediction model was used to screen the DrugBank (a database of experimental and approved drugs): 28 % of the predicted hits were reported in literature as ligands of SULT1E1. From the remaining hits, a selection of nine molecules was subjected to biochemical assay validation and experimental results were in accordance with the in silico prediction of SULT1E1 inhibitors and substrates, thus affirming our prediction hypotheses.
J. Al-Asri, E. Fazekas, G. Lehoczki, A. Perdih, C. Görick, M. F. Melzig, G. Gyémánt, G. Wolber, and J. Mortier. From carbohydrates to drug-like fragments: Rational development of novel α-amylase inhibitors, Bioorg Med Chem, 23(20):6725-6732, 2015.
Links:
[doi:10.1016/j.bmc.2015.09.007]
[show BibTeX]
[show abstract]
x
@article{RN181,
author = {Al-Asri, Jamil and Fazekas, Erika and
Lehoczki, Gábor and Perdih, Andrej and
Görick, Cornelia and Melzig, Matthias F.
and Gyémánt, Gyöngyi and Wolber,
Gerhard and Mortier, Jérémie},
title = {From carbohydrates to drug-like
fragments: Rational development of novel
α-amylase inhibitors},
journal = {Bioorganic & Medicinal Chemistry},
volume = {23},
number = {20},
pages = {6725-6732},
abstract = {Starch catabolism leading to high glucose
level in blood is highly problematic in
chronic metabolic diseases, such as type
II diabetes and obesity. α-Amylase
catalyzes the hydrolysis of starch,
increasing blood sugar concentration. Its
inhibition represents a promising
therapeutic approach to control
hyperglycaemia. However, only few
drug-like molecule inhibitors without
sugar moieties have been discovered so
far, and little information on the
enzymatic mechanism is available. This
work aims at the discovery of novel small
α-amylase binders using a systematic in
silico methodology. 3D-pharmacophore-based
high throughput virtual screening of small
compounds libraries was performed to
identify compounds with high α-amylase
affinity. Twenty-seven compounds were
selected and biologically tested,
revealing IC50 values in the micromolar
range and ligand efficiency higher than
the one of the bound form of acarbose,
which is used as a reference for
α-amylase inhibition.},
keywords = {α-Amylase inhibition Fragment-based drug
design Virtual screening Pharmacophore
model Hyperglycaemia Obesity Type II
diabetes},
ISSN = {0968-0896},
DOI = {10.1016/j.bmc.2015.09.007},
url = {http://www.sciencedirect.com/science/article/pii/S0968089615300304
http://ac.els-cdn.com/S0968089615300304/1-s2.0-S0968089615300304-main.pdf?_tid= 975ce9ee-a00b-11e5-8116-00000aab0f6b&acdnat=1449840915_65cd6dd9bfdd7bab4bc7170cedef1f08},
year = {2015},
type = {Journal Article}
}
x
From carbohydrates to drug-like fragments: Rational development of novel α-amylase inhibitors
Starch catabolism leading to high glucose level in blood is highly problematic in chronic metabolic diseases, such as type II diabetes and obesity. α-Amylase catalyzes the hydrolysis of starch, increasing blood sugar concentration. Its inhibition represents a promising therapeutic approach to control hyperglycaemia. However, only few drug-like molecule inhibitors without sugar moieties have been discovered so far, and little information on the enzymatic mechanism is available. This work aims at the discovery of novel small α-amylase binders using a systematic in silico methodology. 3D-pharmacophore-based high throughput virtual screening of small compounds libraries was performed to identify compounds with high α-amylase affinity. Twenty-seven compounds were selected and biologically tested, revealing IC50 values in the micromolar range and ligand efficiency higher than the one of the bound form of acarbose, which is used as a reference for α-amylase inhibition.
M. Bermudez, C. Rakers, and G. Wolber. Structural characteristics of the allosteric binding site represent a key to subtype selective modulators of muscarinic acetylcholine receptors, Mol Inf, 34(8):526-530, 2015.
Links:
[doi:10.1002/minf.201500025]
[show BibTeX]
[show abstract]
x
@article{RN178,
author = {Bermudez, Marcel and Rakers, Christin and
Wolber, Gerhard},
title = {Structural characteristics of the
allosteric binding site represent a key to
subtype selective modulators of muscarinic
acetylcholine receptors},
journal = {Molecular Informatics},
volume = {34},
number = {8},
pages = {526-530},
abstract = {The high conservation of the orthosteric
acetylcholine binding site of muscarinic
receptors (MAChR) represents a
considerable challenge in terms of
designing subtype selective drugs. A
promising approach to gain subtype
selectivity is to include allosteric or
dualsteric targeting that aims to address
more specific extracellular binding sites.
Despite recent advances in crystallography
of G protein coupled receptors (GPCRs),
structural information for all 5 MAChR
subtypes is not yet available. Here we
report structural models of the active and
the inactive receptor state of all
subtypes derived by homology modelling in
combination with MD simulations. The
comparison of the allosteric binding site
unveils the characteristics for each
subtype on a structural level and
indicates anchor points for rational
design of selective drugs. Additionally,
homology models offer the possibility for
a rational explanation of dualsteric
subtype selectivity, as we show for the M2
over M5 selectivity of the dualsteric
ligands Atr-6-naph and Iper-6-phth.},
keywords = {Drug design Medicinal chemistry Molecular
dynamics Protein models Receptors gpcr},
ISSN = {1868-1751},
DOI = {10.1002/minf.201500025},
url = {http://dx.doi.org/10.1002/minf.201500025
http://onlinelibrary.wiley.com/store/10.1002/minf.201500025/asset/526_ftp.pdf?v= 1&t=ii1pqq6x&s=d5a558605b40783fdaa53e4c2a054424c9266926},
year = {2015},
type = {Journal Article}
}
x
Structural characteristics of the allosteric binding site represent a key to subtype selective modulators of muscarinic acetylcholine receptors
The high conservation of the orthosteric acetylcholine binding site of muscarinic receptors (MAChR) represents a considerable challenge in terms of designing subtype selective drugs. A promising approach to gain subtype selectivity is to include allosteric or dualsteric targeting that aims to address more specific extracellular binding sites. Despite recent advances in crystallography of G protein coupled receptors (GPCRs), structural information for all 5 MAChR subtypes is not yet available. Here we report structural models of the active and the inactive receptor state of all subtypes derived by homology modelling in combination with MD simulations. The comparison of the allosteric binding site unveils the characteristics for each subtype on a structural level and indicates anchor points for rational design of selective drugs. Additionally, homology models offer the possibility for a rational explanation of dualsteric subtype selectivity, as we show for the M2 over M5 selectivity of the dualsteric ligands Atr-6-naph and Iper-6-phth.
M. Bermudez, and G. Wolber. Structure vs. function - the impact of computational methods on the discovery of specific GPCR-ligands, Bioorg Med Chem, 23(14):3907-3912, 2015.
Links:
[doi:10.1016/j.bmc.2015.03.026]
[show BibTeX]
[show abstract]
x
@article{RN171,
author = {Bermudez, Marcel and Wolber, G.},
title = {Structure vs. function - the impact of
computational methods on the discovery of
specific GPCR-ligands},
journal = {Bioorganic & Medicinal Chemistry},
volume = {23},
number = {14},
pages = {3907-3912},
abstract = {Over the past decades, computational
methods have become invaluable for drug
design campaigns but also as auxiliary
tool for structural biology. The
combination of experimental and in silico
methods in the field of G protein coupled
receptors (GPCRs) is indispensable.
Despite recent groundbreaking achievements
in GPCR crystallography, structural
information for the vast majority of this
physiologically important protein class is
only accessible through homology models.
Since the understanding of the
conformational changes resulting in
multiple activation pathways is
incomplete, the design of specific GPCR
modulating drugs remains a major
challenge. However, due to the highly
interdisciplinary requirements for the
investigation of receptor function and the
necessity of joining scientist from
different fields, computational approaches
gain importance in rationalizing and
illustrating certain specific effects. In
silico methods, such as molecular dynamics
(MD) simulations, pharmacophore modeling
or docking, proved to be suitable to
complement experimental approaches. In
this review, we highlight recent examples
of in silico studies that were
successfully applied in the field of GPCR
research. Those approaches follow two main
goals: Firstly, structural investigations
that help to understand the receptor
function and the characterization of
ligand binding and secondly the
identification of novel GPCR modulators as
potential drugs.},
keywords = {gpcr},
DOI = {10.1016/j.bmc.2015.03.026},
url = {http://ac.els-cdn.com/S0968089615002102/1-s2.0-S0968089615002102-main.pdf?_tid=94b05366-a00b-11e5-866f-00000aacb35f&acdnat=1449840910_d9e103adde14b947c8c62833dffbd577},
year = {2015},
type = {Journal Article}
}
x
Structure vs. function - the impact of computational methods on the discovery of specific GPCR-ligands
Over the past decades, computational methods have become invaluable for drug design campaigns but also as auxiliary tool for structural biology. The combination of experimental and in silico methods in the field of G protein coupled receptors (GPCRs) is indispensable. Despite recent groundbreaking achievements in GPCR crystallography, structural information for the vast majority of this physiologically important protein class is only accessible through homology models. Since the understanding of the conformational changes resulting in multiple activation pathways is incomplete, the design of specific GPCR modulating drugs remains a major challenge. However, due to the highly interdisciplinary requirements for the investigation of receptor function and the necessity of joining scientist from different fields, computational approaches gain importance in rationalizing and illustrating certain specific effects. In silico methods, such as molecular dynamics (MD) simulations, pharmacophore modeling or docking, proved to be suitable to complement experimental approaches. In this review, we highlight recent examples of in silico studies that were successfully applied in the field of GPCR research. Those approaches follow two main goals: Firstly, structural investigations that help to understand the receptor function and the characterization of ligand binding and secondly the identification of novel GPCR modulators as potential drugs.
R. B. El-Houri, D. E. Kotowska, K. B. Christensen, S. Bhattacharya, N. Oksbjerg, G. Wolber, K. Kristiansen, and L. P. Christensen. Polyacetylenes from carrots (Daucus carota) improve glucose uptake in vitro in adipocytes and myotubes, Food & Function, 6(7):2135-44, 2015.
Links:
[doi:10.1039/C5FO00223K]
[show BibTeX]
[show abstract]
x
@article{RN175,
author = {El-Houri, Rime Bahij and Kotowska, Dorota
Ewa and Christensen, Kathrine Bisgaard and
Bhattacharya, Sumangala and Oksbjerg,
Niels and Wolber, Gerhard and Kristiansen,
Karsten and Christensen, Lars Porskjær},
title = {Polyacetylenes from carrots (Daucus
carota) improve glucose uptake in vitro in
adipocytes and myotubes},
journal = {Food & Function},
volume = {6},
number = {7},
pages = {2135-44},
abstract = {A dichloromethane (DCM) extract of carrot
roots was found to stimulate
insulin-dependent glucose uptake (GU) in
adipocytes in a dose dependent manner.
Bioassay-guided fractionation of the DCM
extract resulted in the isolation of the
polyacetylenes falcarinol and
falcarindiol. Both polyacetylenes were
able to significantly stimulate basal
and/or insulin-dependent GU in 3T3-L1
adipocytes and porcine myotube cell
cultures in a dose-dependent manner.
Falcarindiol increased peroxisome
proliferator-activated receptor
(PPAR)γ-mediated transactivation
significantly at concentrations of 3, 10
and 30 μM, while PPARγ-mediated
transactivation by falcarinol was only
observed at 10 μM. Docking studies
accordingly indicated that falcarindiol
binds to the ligand binding domain of
PPARγ with higher affinity than
falcarinol and that both polyacetylenes
exhibit characteristics of PPARγ partial
agonists. Falcarinol was shown to inhibit
adipocyte differentiation as evident by
gene expression studies and Oil Red O
staining, whereas falcarindiol did not
inhibit adipocyte differentiation, which
indicates that these polyacetylenes have
distinct modes of action. The results of
the present study suggest that falcarinol
and falcarindiol may represent scaffolds
for novel partial PPARγ agonists with
possible antidiabetic properties.},
DOI = {10.1039/C5FO00223K},
url = {http://pubs.rsc.org/en/content/articlepdf/2015/fo/c5fo00223k},
year = {2015},
type = {Journal Article}
}
x
Polyacetylenes from carrots (Daucus carota) improve glucose uptake in vitro in adipocytes and myotubes
A dichloromethane (DCM) extract of carrot roots was found to stimulate insulin-dependent glucose uptake (GU) in adipocytes in a dose dependent manner. Bioassay-guided fractionation of the DCM extract resulted in the isolation of the polyacetylenes falcarinol and falcarindiol. Both polyacetylenes were able to significantly stimulate basal and/or insulin-dependent GU in 3T3-L1 adipocytes and porcine myotube cell cultures in a dose-dependent manner. Falcarindiol increased peroxisome proliferator-activated receptor (PPAR)γ-mediated transactivation significantly at concentrations of 3, 10 and 30 μM, while PPARγ-mediated transactivation by falcarinol was only observed at 10 μM. Docking studies accordingly indicated that falcarindiol binds to the ligand binding domain of PPARγ with higher affinity than falcarinol and that both polyacetylenes exhibit characteristics of PPARγ partial agonists. Falcarinol was shown to inhibit adipocyte differentiation as evident by gene expression studies and Oil Red O staining, whereas falcarindiol did not inhibit adipocyte differentiation, which indicates that these polyacetylenes have distinct modes of action. The results of the present study suggest that falcarinol and falcarindiol may represent scaffolds for novel partial PPARγ agonists with possible antidiabetic properties.
R. B. El-Houri, J. Mortier, M. S. Murgueitio, G. Wolber, and L. P. Christensen. Identification of PPARγ Agonists from Natural Sources Using Different In Silico Approaches, Planta Med, 81(06):488-494, 2015.
Links:
[doi:10.1055/s-0034-1383119]
[show BibTeX]
[show abstract]
x
@article{RN151,
author = {El-Houri, Rime B. and Mortier, Jérémie
and Murgueitio, Manuela S. and Wolber,
Gerhard and Christensen, Lars P.},
title = {Identification of PPARγ Agonists from
Natural Sources Using Different In Silico
Approaches},
journal = {Planta Medica},
volume = {81},
number = {06},
pages = {488-494},
abstract = {Peroxisome proliferator-activated
receptor γ plays an important role in
lipid and glucose homeostasis and is the
target of many drug discovery
investigations because of its role in
diseases such as type 2 diabetes.
Activation of peroxisome
proliferator-activated receptor γ by
agonists leads to a conformational change
in the ligand-binding domain altering the
transcription of several target genes
involved in glucose and lipid metabolism,
resulting in, for example, facilitation of
glucose and lipid uptake and amelioration
of insulin resistance, and other effects
that are important in the treatment of
type 2 diabetes. Peroxisome
proliferator-activated receptor γ partial
agonists are compounds with diminished
agonist efficacy compared to full
agonists; however, they maintain the
antidiabetic effect of full agonists but
do not induce the same magnitude of side
effects. This mini-review gives a short
introduction to in silico screening
methods and recent research advances using
computational approaches to identify
peroxisome proliferator-activated receptor
γ agonists, especially partial agonists,
from natural sources and how these ligands
bind to the peroxisome
proliferator-activated receptor γ in
order to better understand their
biological effects.},
ISSN = {0032-0943},
DOI = {10.1055/s-0034-1383119},
url = {https://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-0034-1383119.pdf},
year = {2015},
type = {Journal Article}
}
x
Identification of PPARγ Agonists from Natural Sources Using Different In Silico Approaches
Peroxisome proliferator-activated receptor γ plays an important role in lipid and glucose homeostasis and is the target of many drug discovery investigations because of its role in diseases such as type 2 diabetes. Activation of peroxisome proliferator-activated receptor γ by agonists leads to a conformational change in the ligand-binding domain altering the transcription of several target genes involved in glucose and lipid metabolism, resulting in, for example, facilitation of glucose and lipid uptake and amelioration of insulin resistance, and other effects that are important in the treatment of type 2 diabetes. Peroxisome proliferator-activated receptor γ partial agonists are compounds with diminished agonist efficacy compared to full agonists; however, they maintain the antidiabetic effect of full agonists but do not induce the same magnitude of side effects. This mini-review gives a short introduction to in silico screening methods and recent research advances using computational approaches to identify peroxisome proliferator-activated receptor γ agonists, especially partial agonists, from natural sources and how these ligands bind to the peroxisome proliferator-activated receptor γ in order to better understand their biological effects.
S. Grosskopf, C. Eckert, C. Arkona, S. Radetzki, K. Böhm, U. Heinemann, G. Wolber, J. v. Kries, W. Birchmeier, and J. Rademann. Selective inhibitors of the protein tyrosine phosphatase SHP2 block cellular motility and growth of cancer cells in-vitro and in-vivo, ChemMedChem, 10(5):815-826, 2015.
Links:
[doi:10.1002/cmdc.201500015]
[show BibTeX]
[show abstract]
x
@article{RN172,
author = {Grosskopf, Stefanie and Eckert, Chris and
Arkona, Christoph and Radetzki, Silke and
Böhm, Kerstin and Heinemann, Udo and
Wolber, Gerhard and Kries, Jens-Peter von
and Birchmeier, Walter and Rademann,
Jörg},
title = {Selective inhibitors of the protein
tyrosine phosphatase SHP2 block cellular
motility and growth of cancer cells
in-vitro and in-vivo},
journal = {Chemmedchem},
volume = {10},
number = {5},
pages = {815-826},
abstract = {Selective inhibitors of the protein
tyrosine phosphatase SHP2 (PTPN11), an
enzyme, deregulated in numerous human
tumors, were generated by combination of
chemical synthesis with structure-based
rational design. 70
pyridazolon-4-ylidene-hydrazinyl-benzene
sulfonates were prepared and evaluated in
enzyme assays. Binding modes of active
inhibitors were simulated in silico using
a newly generated crystal structure of
SHP2. The most powerful molecule 25
(GS-493) inhibited SHP2 with an IC50 value
of 71 ± 15 nM in the enzyme assay and was
29- and 45-fold more active than against
related SHP1 and PTP1B. In cell culture
experiments 25 blocked HGF-stimulated
epithelial-mesenchymal transition of human
pancreatic tumor cells HPAF as indicated
by the reduction of minimum neighbor
distances of cells. Moreover, 25 inhibited
cell colony formation of the non-small
cell lung cancer cell line LXFA 526L in
soft agar. Finally, it inhibited tumor
growth in a murine xenograft model. Thus,
the novel specific molecule strengthens
the hypothesis that SHP2 is a relevant
protein target for the inhibition of
mobility and invasiveness of cancer
cells.},
DOI = {10.1002/cmdc.201500015},
url = {http://onlinelibrary.wiley.com/doi/10.1002/cmdc.201500015/abstract
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cmdc.201500015},
year = {2015},
type = {Journal Article}
}
x
Selective inhibitors of the protein tyrosine phosphatase SHP2 block cellular motility and growth of cancer cells in-vitro and in-vivo
Selective inhibitors of the protein tyrosine phosphatase SHP2 (PTPN11), an enzyme, deregulated in numerous human tumors, were generated by combination of chemical synthesis with structure-based rational design. 70 pyridazolon-4-ylidene-hydrazinyl-benzene sulfonates were prepared and evaluated in enzyme assays. Binding modes of active inhibitors were simulated in silico using a newly generated crystal structure of SHP2. The most powerful molecule 25 (GS-493) inhibited SHP2 with an IC50 value of 71 ± 15 nM in the enzyme assay and was 29- and 45-fold more active than against related SHP1 and PTP1B. In cell culture experiments 25 blocked HGF-stimulated epithelial-mesenchymal transition of human pancreatic tumor cells HPAF as indicated by the reduction of minimum neighbor distances of cells. Moreover, 25 inhibited cell colony formation of the non-small cell lung cancer cell line LXFA 526L in soft agar. Finally, it inhibited tumor growth in a murine xenograft model. Thus, the novel specific molecule strengthens the hypothesis that SHP2 is a relevant protein target for the inhibition of mobility and invasiveness of cancer cells.
C. Meinguet, C. Bruyère, R. Frédérick, V. Mathieu, C. Vancraeynest, L. Pochet, J. Laloy, J. Mortier, G. Wolber, R. Kiss, B. Masereel, and J. Wouters. 3D-QSAR, design, synthesis and characterization of trisubstituted harmine derivatives with in vitro antiproliferative properties, Eur J Med Chem, 94:45-55, 2015.
Links:
[doi:10.1016/j.ejmech.2015.02.044]
[show BibTeX]
[show abstract]
x
@article{RN169,
author = {Meinguet, Céline and Bruyère, Céline
and Frédérick, Raphaël and Mathieu,
Véronique and Vancraeynest, Christelle
and Pochet, Lionel and Laloy, Julie and
Mortier, Jérémie and Wolber, Gerhard and
Kiss, Robert and Masereel, Bernard and
Wouters, Johan},
title = {3D-QSAR, design, synthesis and
characterization of trisubstituted harmine
derivatives with in vitro
antiproliferative properties},
journal = {European Journal of Medicinal Chemistry},
volume = {94},
pages = {45-55},
abstract = {Apolar trisubstituted derivatives of
harmine show high antiproliferative
activity on diverse cancer cell lines.
However, these molecules present a poor
solubility making these compounds poorly
bioavailable. Here, new compounds were
synthesized in order to improve solubility
while retaining antiproliferative
activity. First, polar substituents have
shown a higher solubility but a loss of
antiproliferative activity. Second, a
Comparative Molecular Field Analysis
(CoMFA) model was developed, guiding the
design and synthesis of eight new
compounds. Characterization has underlined
the in vitro antiproliferative character
of these compounds on five cancerous cell
lines, combining with a high solubility at
physiological pH, making these molecules
druggable. Moreover, targeting glioma
treatment, human intestinal absorption and
blood brain penetration have been
calculated, showing high absorption and
penetration properties.},
keywords = {Harmine derivatives Antiproliferative
activity Cytostatic activity Comparative
Molecular Field Analysis},
ISSN = {0223-5234},
DOI = {10.1016/j.ejmech.2015.02.044},
url = {http://www.sciencedirect.com/science/article/pii/S0223523415001403
http://ac.els-cdn.com/S0223523415001403/1-s2.0-S0223523415001403-main.pdf?_tid= f547ccae-c00a-11e4-8b31-00000aab0f01&acdnat=1425211582_cb6d118f6d7adec74c3f2a725b13dbdb},
year = {2015},
type = {Journal Article}
}
x
3D-QSAR, design, synthesis and characterization of trisubstituted harmine derivatives with in vitro antiproliferative properties
Apolar trisubstituted derivatives of harmine show high antiproliferative activity on diverse cancer cell lines. However, these molecules present a poor solubility making these compounds poorly bioavailable. Here, new compounds were synthesized in order to improve solubility while retaining antiproliferative activity. First, polar substituents have shown a higher solubility but a loss of antiproliferative activity. Second, a Comparative Molecular Field Analysis (CoMFA) model was developed, guiding the design and synthesis of eight new compounds. Characterization has underlined the in vitro antiproliferative character of these compounds on five cancerous cell lines, combining with a high solubility at physiological pH, making these molecules druggable. Moreover, targeting glioma treatment, human intestinal absorption and blood brain penetration have been calculated, showing high absorption and penetration properties.
J. Mortier, E. K. Nyakatura, O. Reimann, S. Huhmann, J. O. Daldrop, C. Baldauf, G. Wolber, M. S. Miettinen, and B. Koksch. Coiled-coils in phage display screening: Insight into exceptional selectivity provided by molecular dynamics, J Chem Inf Model, 55(3):495-500, 2015.
Links:
[doi:10.1021/ci500689c]
[show BibTeX]
[show abstract]
x
@article{RN160,
author = {Mortier, J. and Nyakatura, E. K. and
Reimann, O. and Huhmann, S. and Daldrop,
J. O. and Baldauf, C. and Wolber, G. and
Miettinen, M. S. and Koksch, B.},
title = {Coiled-coils in phage display screening:
Insight into exceptional selectivity
provided by molecular dynamics},
journal = {Journal of Chemical Information and
Modeling},
volume = {55},
number = {3},
pages = {495-500},
note = {Mortier, Jeremie Nyakatura, Elisabeth K
Reimann, Oliver Huhmann, Susanne Daldrop,
Jan O Baldauf, Carsten Wolber, Gerhard
Miettinen, Markus S Koksch, Beate ENG
2015/02/05 06:00 J Chem Inf Model. 2015
Feb 12.},
abstract = {Involved in numerous key biological
functions, protein helix-helix
interactions follow a well-defined
intermolecular recognition pattern. The
characteristic structure of the
alpha-helical coiled-coil allows for the
specific randomization of clearly defined
interaction partners within heteromeric
systems. In this work, a rationally
designed heterodimeric coiled-coil was
used to investigate potential factors
influencing the sequence selectivity in
interhelical interactions.},
ISSN = {1549-960X (Electronic) 1549-9596
(Linking)},
DOI = {10.1021/ci500689c},
url = {http://www.ncbi.nlm.nih.gov/pubmed/25648076
http://pubs.acs.org/doi/pdfplus/10.1021/ci500689c},
year = {2015},
type = {Journal Article}
}