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Exon inclusion modulates conformational plasticity and autoinhibition of the intersectin 1 SH3A domain

Gerth_Struc_TOC_pub_079

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Gerth, F.; Jaepel, M.; Sticht, J.; Kuropka, B.; Schmitt, X. J., Driller, J. H.; Loll, B.; Wahl, M. C.; Pagel, K.; Haucke, V.; Freund, C.* – 2019

The scaffolding protein intersectin 1 plays important roles in clathrin-mediated endocytosis and in the replenishment of release-ready synaptic vesicles (SV). Two splice variants of intersectin's SH3A domain are expressed in the brain, and association of the neuron-specific variant with synapsin I has been shown to enable sustained neurotransmission and to be regulated by an adjacent C-terminal motif. Here, we demonstrate that the ubiquitously expressed short SH3A variant of intersectin 1 interacts with an N-terminal intramolecular sequence that operates synergistically with the C-terminal motif. NMR spectroscopic investigations show that the five-amino acid insertion into the β strand 2 of the neuronal SH3A variant introduces conformational plasticity incompatible with binding of the N-terminal sequence. The difference in the autoregulatory mechanism of the domain's variants differentially affects its synaptic binding partners, thereby establishing alternative splicing in conjunction with autoinhibitory motif variation as a mechanism to regulate protein interaction networks.

Title
Exon inclusion modulates conformational plasticity and autoinhibition of the intersectin 1 SH3A domain
Author
Gerth, F.; Jaepel, M.; Sticht, J.; Kuropka, B.; Schmitt, X. J., Driller, J. H.; Loll, B.; Wahl, M. C.; Pagel, K.; Haucke, V.; Freund, C.*
Date
2019
Identifier
10.1016/j.str.2019.03.020
Source(s)
Citation
Structure 2019, 27, 977–987.
Type
Text
masses4masses
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