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Exploring the Potential of Dendritic Polyglycerol Detergents for Protein Analysis by Mass Spectrometry

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Urner, L. H.; Maier, Y.; Haag, R.; Pagel, K.* – 2019

The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein β-lactoglobulin (BLG).

Title
Exploring the Potential of Dendritic Polyglycerol Detergents for Protein Analysis by Mass Spectrometry
Author
Urner, L. H.; Maier, Y.; Haag, R.; Pagel, K.*
Date
2019
Identifier
10.1007/s13361-018-2063-2
Source(s)
Citation
J. Am. Soc. Mass Spectrom. 2019, 30, 174–180.
Type
Text
masses4masses
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