Collision cross sections of high-mannose N-glycans in commonly observed adduct states - Identification of gas-phase conformers unique to [M-H]- ions
Struwe, W. B. *; Benesch, J. L. P.; Harvey, D. J. and Pagel, K.* – 2015
We report collision cross sections (CCS) of high-mannose N-glycans as [M+Na]+, [M+K]+, [M+H]+, [M+Cl]-, [M+H2PO4]- and [M-H]- ions, measured by drift tube (DT) ion mobility-mass spectrometry (IM-MS) in helium and nitrogen gases. Further analysis using traveling wave (TW) IM-MS reveal the existence of distinct conformers exclusive to [M-H]- ions.