Hydrogel-integrated graphene superstructures (GSSs) represent a promising platform for applications in tissue engineering and regenerative medicine. Graphene, a two-dimensional carbon-based material, possesses remarkable mechanical, thermal, and electrical characteristics, making it a strong candidate for application in biomedicine. Researchers have pursued the integration of graphene with hydrogels, known for their biocompatibility and ability to provide a conducive environment for cellular growth, to craft sophisticated scaffolds tailored to tissue engineering needs. The integration of hydrogels and graphene enables the construction of 3D frameworks that closely mimic the natural extracellular matrix (ECM) found in biological tissues. Hydrogels furnish a biocompatible, well-hydrated environment, while the graphene component bolsters the scaffold's mechanical integrity and electrical conductivity. This amalgamation enhances cellular adhesion, differentiation, and proliferation, thereby facilitating tissue regeneration. A notable advantage of hydrogel-integrated GSSs lies in their capacity to support the growth and differentiation of a variety of cell types such as PC12, MG-63, U-87, and MC3T3-E1 cell lines. Overall, hydrogel-integrated GSSs exhibit great potential for advancing biomimetic tissue engineering and regenerative medicine. The combination of the unique properties of graphene with the biocompatibility of hydrogels enables the development of advanced scaffold systems for tissue regeneration. Further research and development in this domain will play a crucial role in advancing regenerative medicine and the treatment of various diseases and injuries.