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Selected recent work:

  • Ries, J., Kaplan, C., Platonova, E., Eghlidi, H. and Ewers, H. A simple, versatile method for GFP-based single molecule localization microscopy via nanobodies. (2012) Nature Methods DOI: 10.1038/NMETH.1991

    JCI classified as “highly cited paper”, Faculty of 1000 recommended, most downloaded paper at Nature Methods in May 2012, > 17000 times downloaded

  • Mikhaylova M., Cloin, B.M.C., Finan, K., van den Berg, R., Teeuw, J., Kijanka, M.M., Sokolowski, M., Katrukha, E.A., Maidorn, M. Opazo, F., Moutel, S., Vantard, M. Perez, F.,van Bergen en Henegouwen, P.M.P., Hoogenraad, C.C., Ewers, H.*, and Kapitein, L.C.* Resolving bundled microtubules using anti-tubulin nanobodies. (2015) Nature Communications doi: 10.1038/ncomms8933

  • Kaplan, C., Jing, B., Winterflood, C.W., Bridges, A.A., Occhipinti, P., Schmied, J., Grinhagens, S., Gronemeyer, T., Tinnefeld, P., Gladfelter, A.S., Ries, J. and Ewers, H. The absolute arrangement of subunits in cytoskeletal septin filaments in cells measured by fluorescence microscopy. (2015) Nano Letters 15(6):3859-64. doi: 10.1021/acs.nanolett.5b00693

  • Winterflood, C.M., Platonova, E., Albrecht, D. and Ewers H. Simple and robust dual color 3D superresolution microscopy by combined spectral-demixing and bi-plane imaging (2015) Biophysical Journal 109 (1) pp:3-6 doi:10.1016/j.bpj.2015.05.026

  • Kaplan, C. and Ewers, H. Optimized sample preparation for single molecule localization-based superresolution microscopy in yeast. (2015) Nature Protocols 10(7):1007-21. doi: 10.1038/nprot.2015.060

  • Platonova, E., Winterflood, C.M., Junemann, A., Albrecht, D., Faix, J. and Ewers, H. Single-molecule microscopy of molecules tagged with GFP or mRFP derivatives in mammalian cells using nanobody binders. (2015) Methods doi: 10.1016/j.ymeth.2015.06.018

  • Albrecht, D.*, Winterflood C.W.* and Ewers, H. Dual color single particle tracking via nanobodies. (2015) Methods and Applications in Fluorescence 3 024001 doi: 10.1088/2050-6120/3/2/024001

  • Platonova E.*, Winterflood, C.W.* and Ewers, H. A simple method for GFP- and RFP-based dual color single molecule localization microscopy. (2015) ACS Chemical Biology 10(6):1411-6. doi: 10.1021/acschembio.5b00046

  • Winterflood C. W. and Ewers H. Single-molecule localization microscopy using mCherry. (2014) ChemPhysChem 15, 3447 – 3451 doi: 10.1002/cphc.201402423

  • Szklarczyk, O., Gonzales-Segredo, N., Kukura, P., Oppenheim, A., Choquet, D., Sandoghdar, V., Helenius, A., Sbalzarini, I.F. and Ewers, H. Receptor concentration and diffusivity control multivalent binding of SV40 to membrane bilayers. (2013) PLoS Computational Biology 9(11) e1003310 doi: 10.1371/journal.pcbi.1003310

Further work: 

  • Thiede-Stan, N.K., Ristic, Z., Albrecht, D., Tews, B., Ewers, H. and Schwab, M.E. (2015) Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex. Journal of Cell Science doi: 10.1242/jcs.167981

  • Ewers, H.*, Tada, T.*, Petersen, J., Racz, B., Sheng, M. and Choquet, D. (2014) A septin-dependent diffusion barrier at the neck of dendritic spines. PLoS ONE 9(12): e113916. doi:10.1371/journal.pone. 0113916

  • Spillane, K., Ortega-Arroyo, J., de Wit, G., Eggeling, C., Ewers, H., Wallace, M. and Kukura, P. (2014) High-speed single-particle tracking of GM1 in model membranes reveals anomalous diffusion due to interleaflet coupling and molecular pinning. Nano Letters dx.doi.org/10.1021/nl502536u

  • Ries, J., Udayar, V., Soragni, A., Hornemann, S., Nilsson, P., Ewers, H., Riek, R., Hock, C., Aguzzi, A. and Rajendran, L. Superresolution imaging of amyloid fibrils with binding-activated probes (2013) ACS Chemical Neuroscience doi: 10.1021/cn400091m

  • Kleine Borgmann LAK, Ries J, Ewers H, Ulbrich MH*, Graumann PL*. The bacterial SMC complex displays a novel mode of interaction with the chromosome. (2013) Cell Reports 3(5) 1483-92 doi: 10.1016/j.celrep.2013.04.005

  • McEvoy, A.L., Hoi, H., Bates, M., Platonova, E., Cranfill, P.J., Davidson, M.J., Ewers, H., Liphardt, J. and Campbell, R.E., mMaple: a photoconvertible fluorescent protein for use in multiple imaging modalities. (2012) PLoS One, 7(12): e51314. doi: 10.1371/journal.pone.0051314

  • Schoen, I., Ries, J., Klotzsch, E., Vogel, V*and Ewers, H.* Binding-activated localization microscopy (BALM). (2011) Nano Letters 11, 4008-11 doi: 10.1021/nl2025954

  • Ries, J., Bayer, M., Csucs, G., Dirkx, R., Solimena, M., Ewers, H. & Schwille, P. Automated suppression of sample-related artifacts in Fluorescence Correlation Spectroscopy. (2010) Optics Express 18: 11073-82

  • Buerli, T., Baer, K., Ewers, H., Sidler, C., Fuhrer,C. and Fritschy, JM. Single particle tracking of alpha7 nicotinic acetylcholine receptors in hippocampal neurons reveals regulated perisynaptic confinement at glutamatergic and GABAergic synapses. (2010) PLOS One 5(7): e11507. doi: 10.1371/journal.pone.0011507

  • Ewers, H.*, Roemer, W.*, Smith, A.E., Bacia, K., Dmitrieff, S., Chai, W., Mancini, R., Kartenbeck, J., Chambon, V., Berland, L., Oppenheim, A., Schwarzmann, G., Feizi, T., Schwille, P., Sens, P., Helenius, A. and Johannes, L. (2010) SV40 binding to its receptor, GM1, induces membrane invagination, tubulation and infection. Nature Cell Biology. 12(1) 11-8. doi: 10.1038/ncb1999

    featured as Research Highlight in Nature Reviews Microbiology

  • Kukura, P., Ewers, H., Muller, C., Renn, A., Helenius, A. and Sandoghdar, V. (2009) High-speed nanoscopic tracking of the position and orientation of a single virus. Nature Methods. 6 (12), 923-7. doi: 10.1038/nmeth.1395

  • Schelhaas, M., Ewers, H., Rajamaki, M. L., Day, P. M., Schiller, J. T. & Helenius, A. (2008) Human papillomavirus type 16 entry: retrograde cell surface transport along actin-rich protrusions, PLoS Pathogens. 4, (9) e1000148. doi: 10.1371/journal.ppat.1000148

  • Fiolka, R., Belyaev, Y., Ewers, H. & Stemmer, A. (2008) Even illumination in total internal reflection fluorescence microscopy using laser light, Microscopy Research and Technique. 71, 45-50. doi: 10.1002/jemt.20527

  • Ewers, H., Jacobsen, V., Klotzsch, E., Smith, A. E., Helenius, A. & Sandoghdar, V. (2007) Label-free optical detection and tracking of single virions bound to their receptors in supported membrane bilayers, Nano Letters. 7, 2263-6. doi: 10.1021/nl070766y

  • Campanero-Rhodes, M. A., Smith, A., Chai, W., Sonnino, S., Mauri, L., Childs, R. A., Zhang, Y., Ewers, H., Helenius, A., Imberty, A. & Feizi, T. (2007) N-glycolyl GM1 ganglioside as a receptor for simian virus 40, J Virol. 81, 12846-58. doi: 10.1128/JVI.01311-07

  • Boiko, T., Vakulenko, M., Ewers, H., Yap, C. C., Norden, C. & Winckler, B. (2007) Ankyrin-dependent and -independent mechanisms orchestrate axonal compartmentalization of L1 family members neurofascin and L1/neuron-glia cell adhesion molecule, J Neuroscience. 27, 590-603. doi: 10.1523/JNEUROSCI.4302-06.2007

  • Chang, M. C., Wisco, D., Ewers, H., Norden, C. & Winckler, B. (2006) Inhibition of sphingolipid synthesis affects kinetics but not fidelity of L1/NgCAM transport along direct but not transcytotic axonal pathways, Mol Cell Neurosci. 31, 525-38. doi: 10.1016/j.mcn.2005.11.006

  • Ewers, H., Smith, A. E., Sbalzarini, I. F., Lilie, H., Koumoutsakos, P. & Helenius, A. (2005) Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes, Proc Natl Acad Sci U S A. 102, 15110-5. doi:10.1073/pnas.0504407102

    Faculty of 1000 recommended 

* equal contribution


  • Geertsema, H. and Ewers, H. (2016) Expansion Microscopy passes its first test. Nature Methods

  • Hell, S.W., Sahl, S.J., Bates, M., Zhuang, X., Heintzmann, R., Booth, M.J., Bewersdorf, J., Shtengel, Hess, H., Tinnefeld, P., Honigmann, A., Jakobs, S., Testa, I.,  Cognet, L.,  Lounis, B., Ewers, H.,  Davis, S.J.,  Eggeling, C., Klenerman, D., Willig, K., Vicidomini, G., Castello, M., Diaspro, A. and Cordes, T. (2015) The 2015 super-resolution microscopy roadmap. J. Phys. D: Appl. Phys. 48 (2015) 443001 (35pp)

  • Ewers, H. (2011) Septin pairs, a complex choreography Journal of Cell Biology 193(6):959-61 doi: 10.1083/jcb.201105076

  • Ewers, H., and Helenius, A. (2011) Lipid-mediated endocytosis Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a004721

  • Thoumine, O., Ewers, H., Heine, M., Groc, L., Frischknecht, R., Giannone, G., Poujol, C., Legros, P., Lounis, B., Cognet, L. & Choquet, D. (2008) Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging, Chem Rev. 108, 1565-87. doi: 10.1021/cr078204m

Book Chapters:

  • Ewers, H. Nano resolution optical imaging through photoactivation localization microscopy (PALM) in: Cellular Imaging Techniques for Neuroscience and Beyond, Elsevier (2012)

  • Ewers, H., and Schelhaas, M., Tracking virus particles for the analysis of cellular membrane dynamics, Methods in Enzymology (2012) 506:63-80      

A full list under: