Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner

neuron2002-cover

neuron2002-cover

Pennetta, G., Hiesinger, P.R., Fabian-Fine, R., Meinertzhagen, I.A., and Bellen H.J. – 2002

Aplysia VAP-33 (VAMP-associated protein) has been previously proposed to be involved in the control of neurotransmitter release. Here, we show that a Drosophila homolog of VAP-33, DVAP-33A, is localized to neuromuscular junctions. Loss of DVAP-33A causes a severe decrease in the number of boutons and a corresponding increase in bouton size. Conversely, presynaptic overexpression of DVAP-33A induces an increase in the number of boutons and a decrease in their size. Gain-of-function experiments show that the presynaptic dose of DVAP-33A tightly modulates the number of synaptic boutons. Our data also indicate that the presynaptic microtubule architecture is severely compromised in DVAP-33A mutants. We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding.

Titel
Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
Verfasser
Pennetta, G., Hiesinger, P.R., Fabian-Fine, R., Meinertzhagen, I.A., and Bellen H.J.
Verlag
Cell Press
Datum
2002-07-18
Kennung
doi: 10.1016/S0896-6273(02)00769-9
Erschienen in
Neuron 35(2): 291-306. Cover article
Sprache
eng
Art
Text
Rechte
© 2002 Cell Press. Published by Elsevier Inc.
NeuroCure