Due to the dynamic Covid-19 situation the talk on 01.12.2021 is cancelled
and will be shifted to the summer semester 2022
Invitation to PC-TC Colloquium
Prof. Dr. Wolfang Parak
CHyN, Universität Hamburg, Hamburg, Germany
"Quantitative particle uptake and intracellular fate"
Wednesday, December 1st 2021, 16:15 hours
Lecture Hall B (B.004), Arnimallee 22, 14195 Berlin
Nanomaterials play a promising role in the advancements of technology and health care. Their nanometric size combined with particular optoelectronic, magnetic, or plasmonic properties makes them suitable candidates for theranostics, biosensing, and bioimaging applications. This led to intensive investigation of the interaction of nanoparticles (NPs) with cells. Whereas most studies are focused on how the physicochemical properties of NPs will influence their uptake by cells, much less is known about their potential excretion from cells. However, to control and manipulate the number of NPs in a cell both, cellular uptake and excretion need to be studied quantitatively. Monitoring the intracellular and extracellular amount of NPs, after residual non-internalized NPs have been removed, over time enables to disentangle the influence of cell proliferation and exocytosis, which are the major pathways for the reduction of NPs per cell. Proliferation depends on the type of cells, and exocytosis depends on the size of the NPs. Examples are given on the role of these two different processes for different cells and NPs.
Reference: Y. Kang, L. Nack, Y. Liu, B. Qi, Y. Huang, Z. Liu, I. Chakraborty, F. Schulz, A. Ahmed, M. Clavo Podeva, F. Hafizi, S. Roy, M. Mutas, M. Holzapfel, C. Sanchez Cano, K. D. Wegner, N. Feliu, W. J. Parak, "Quantitative considerations about the size dependency for cellular entry and excretion of colloidal nanoparticles for different cells", ChemTexts, submitted.