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Dr. Bernhard Loll

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Biochemistry

Erasmus coodinator

Project Leader

Structural Biocatalysis

Address
Takustraße 6
Room 307
14195 Berlin
Fax
+49 30 838 457348

Office hours

Appointment upon agreement

 

Academic Education

Since 2014   Habilitation (venia legendi) in Biochemistry with Prof. Markus C. Wahl, PhD; Freie Universität Berlin
2013   Co-founder of the company “moloX GmbH”
2010 - 2013 Postdoctoral Researcher with Prof. Markus C. Wahl, PhD; Freie Universität Berlin
2005 - 2009 Postdoctoral Researcher with Prof. Dr. Ilme Schlichting; Max-Planck-Institut für Medizinische Forschung,  Dept. Biomolecular Mechanisms
2000 - 2005 PhD in Biochemistry with Prof. Dr. Wolfram Saenger; Freie Universität Berlin
1999                   Diplom in Chemistry with Prof. Georg E. Schulz; Albert-Ludwigs Universität Freiburg im Breisgau
1994 - 1999 Studies of Chemistry, Albert-Ludwigs Universität Freiburg im Breisgau


Editorial board member

Since 2019    Molecules: Section "Chemical Biology"                                                                                                                                 


Awards and Honors

2006 Recipient of the GE & Science Prize for Young Life Scientists “Europe” category winner awarded by the American Association for the Advancement of Science (AAAS)
2005                      Dr. rer. nat. summa cum laude

                  

 


Research

Since many raw materials become rare and consequently expensive, bio-based products accessed by biotechnological approaches are getting more and more competitive and attractive. By integration of biosynthetic pathways in microorganisms, organic molecules can be produced. This is termed “white biotechnology” since cells are used as micro-factories using biomass as feedstock rather than petrochemicals.

One class of enzymes of interest are terpene cyclases. Terpenes constitute the largest and most diverse one present in almost all species, including plants, fungi and bacteria. Because of their immense structural and bioactive diversity, terpenes are present in many modern strands of the chemical, biotechnological and pharmaceutical industry. Most of these complex polycyclic molecules are difficult to access by chemical synthesis. The variety of terpene molecules with elaborate stereochemistry and regioselectivity is generated by terpene synthases, sometimes referred to as terpene cyclases. By site-directed mutagenesis amino acids in the active site of terpene cyclases can be altered to obtain novel products compared to the native product. Moreover the novel products can be further modified and functionalized by classical organic chemistry. We are aiming to elucidate high resolution structures of various terpene cyclases in different states along the reaction coordinate to deepen our understanding of the cyclization mechanism. The obtained results will help in the design of variant terpene cyclases.


 

Methods: We overexpress proteins in bacteria or insect cells. They are subsequently purified and biophysical characterized (multi-angle-light scattering, thermofluor analysis, etc). To gain structural information of biomacromolecules, we perform crystallization. By X-ray crystallography, we obtain structures that are further analyzed investigated. Structure-based mutagenesis is employed to alter and thereby to optimize catalysis. 



Cooperation partners:

Prof. Dr. Mathias Christmann, Freie Universität Berlin

Prof. Dr. Andreas Ziegler, Charité Berlin

Prof. Dr. Thomas Brück, Technische Universität München

Prof. Dr. Dan Major, Bar Ilan University, Israel

Prof. Dr. Robert Kourist, Technische Universität Graz

Prof. Dr. Jeroen S. Dickschat, Universität Bonn


 

Selected publications:

Driller, R., Garbe, D., Mehlmer, N., Fuchs, M., Raz, K., Major, D. T., Brück, T. and Loll, B. Current understanding and biotechnological application of the bacterial diterpene synthase CotB2. (2019) Beilstein J. Org. Chem., 15, 2355–2368

Driller, R., Janke, S., Fuchs, M., Warner, E., Mhashal, A.R., Major, D.T., Christmann, M., Brück, T., Loll, B. Towards a comprehensive understanding of the structural dynamics of a bacterial diterpene synthase during catalysis. (2018) Nature Comm., 91, 3971

Semsary, S., Crnovčić, I., Driller, R., Vater, J., Loll, B., Keller, U. Ketonization of proline residues in the peptide chains of actinomycins by a 4-Oxoproline synthase. (2018) ChemBioChem, 19(7), 706-715.

Lorenzen, J., Driller, R., Waldow, A., Qoura, F., Loll, B., Brück, T. Rhodococcus erythropolis Oleate Hydratase: a New Member in the Oleate Hydratase Family Tree - Biochemical and Structural Studies. (2018) ChemCatChem, 10, 407-414.

Görner, C., Schrepfer, P., Redai, V., Haslbeck, M., Wallrapp, F., Loll, B., Eisenreich, W., Brück, T. Identification, characterization and molecular adaptation of class I redox systems for the production of hydroxylated diterpenoids. (2016) Microb. Cell Fact., 15(1):86.

Sommer, B., von Moeller, H., Haack, M., Qoura, F., Langner, C., Bourenkov, G., Garbe, D., Loll, B., Brück, T. Detailed structure-function correlations of Bacillus subtilis acetolactate synthase. (2015) ChemBioChem., 16, 110-118.

Janke, R., Görner, C., Hirte, M., Brück, T., Loll, B. Crystal structure of the first bacterial diterpene cyclase: CotB2. (2014) Acta Crystallogr. D Biol. Crystallogr. D70, 1528-1537.

Brück, T., Kourist, R., Loll, B. Production of macrocyclic sesqui- and diterpenes in heterologous microbial hosts: A systems approach to harness nature’s molecular diversity. (2014) ChemCatChem. 6(5), 1142-1165.


Funding:

 


Selected publications

Peer reviewed journals

The following link provides a list of publications of Bernhard Loll:

http://www.ncbi.nlm.nih.gov/pubmed/?term=loll_b


Book chapters

Ziegler, A., Loll, B., Misselwitz, R., Uchanska-Ziegler B. Implications of structural and thermodynamic studies of HLA-B27 subtypes exhibiting differential association with ankylosing spondylitis. in Advances in Experimental Medicine and Biology. Eds. López-Larrea, C. and Díaz-Peña, R. (2009), 177-195.

Loll, B., Biesiadka, J. Saenger, W. in Handbook of Metalloproteins Vol. 4, DOI: 10.1002/0470028637.met211, Eds. A. Messerschmidt, W. Bode, M. Cygler, Wiley,Chichester, (2007).

Saenger, W., Biesiadka, J., Loll, B. in Handbook of Metalloproteins Vol. 4, DOI: 10.1002/0470028637.met210, Eds. A. Messerschmidt, W. Bode, M. Cygler, Wiley,Chichester, (2007).