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M.Phil. Yanan Sun

_Yanan

FU Berlin

Fachbereich BCP

Institut für Pharmazie

Doktorandin

Adresse
FU Berlin
Institut für Pharmazie
Königin-Luise-Straße 2+4
Raum 274
14195 Berlin
Homepage

Human sulfotransferases (SULTs) are phase ІІ enzymes which catalyze the transferring of sulfo-moieties to alcoholic or amino acceptors. In my study, we successfully expressed 14 human SULTs using recombinant fission yeast cells. A modified variant of sulfotransferase assay was developed that employs permeabilized fission yeast cells (enzyme bags). The enzymatic functionality of SULT4A1 and SULT6B1 was first demonstrated in our research. Currently, an optimized approach for reaching a higher metabolite yield with lower cost are undergoing developing. Further multi-substrates screening or up-scaling will be performed based on the optimized method.