(Left) Pathway of amyloid formation (nucleation-dependent fibril formation). (Right) Negative staining transmission electron microscopy (TEM) images of an NFGAIL solution showing the morphology of the resulting amyloid fibrils.

(Left) Pathway of amyloid formation (nucleation-dependent fibril formation). Within this pathway, an increase in the mass of amyloid fibrils is observed during fibrillation process, producing a sigmoid kinetic curve which comprises nucleation (lag phase) and elongation (growth phase). (Right) Negative staining transmission electron microscopy (TEM) images of an NFGAIL solution (4 mM) in ammonium acetate showing the morphology of the resulting amyloid fibrils.