Quantification of superoxide and hydrogen peroxide in leaves

Juszczak I, Baier M – 2014

Reactive oxygen species (ROS) are produced in plants under both non-stressful and stressful conditions. Various histochemical staining methods have been developed and are widely used to visualize ROS accumulation sites. In contrast to qualitative analysis, quantification of ROS has been time- and labor consuming. As a consequence, the number of samples, which could be analyzed in parallel, has been limited. To overcome this problem, we introduce an improved semiquantitative method, in which ROS levels are quantified after histochemical staining in plant organs with the digital image analysis package ImageJ.

Title
Quantification of superoxide and hydrogen peroxide in leaves
Author
Juszczak I, Baier M
Publisher
Humana Press
Date
2014
Identifier
Doi: 10.1007/978-1-4939-0844-8_16
Appeared in
Methods in Molecular Biology (Clifton, N.J.), 1166: 217-24
Language
eng
Type
Text
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