Preparation of developing and adult Drosophila brains and retinae for live imaging.

Williamson, W.R. and Hiesinger, P.R. – 2010

The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies. All protocols are optimized for the live culture of the preparations. However, we also present the conditions for fixed tissue immunohistochemistry where applicable. Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber. Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

Title
Preparation of developing and adult Drosophila brains and retinae for live imaging.
Author
Williamson, W.R. and Hiesinger, P.R.
Publisher
MYJoVE Corporation
Keywords
Neuroscience, Issue 37, dissection technique, eye disc, brain culture, photoreceptor, confocal microscopy
Date
2010-03-15
Identifier
doi: 10.3791/1936
Appeared in
J Vis Exp (37):
Language
eng
Type
Text
Rights
© 2010, Journal of Visualized Experiments
NeuroCure