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MSc Pharmaceutical Science David Busse


Institute of Pharmacy

Clinical Pharmacy & Biochemistry

Freie Universität Berlin

Kelchstr. 31
12169 Berlin

Since 07/2018

Doctoral student at the Graduate Research Training Program “PharMetrX: Pharmacometrics & Computational Disease Modelling“ at the Dept. of Clinical Pharmacy & Biochemistry supervised by Prof. Dr. Charlotte Kloft at the Freie Universitaet Berlin

09/2016 – 06/2018

Traineeship AstraZeneca AB, Sweden in Innovative Medicines and Early Development

05/2016– 10/2014

M.Sc. Pharmaceutical Science at Westfälische Wilhelms-Universität Münster, Germany; 3-month internship at Bayer AG, Germany; master thesis at AstraZeneca AB, Sweden

10/2011 – 07/2014

B.Sc. Molecular Biology at Georg-August-Universität Göttingen, Germany; Erasmus semester at Göteborgs Universitet, Sweden

Research abstract – David Busse


Optimisation of antiinfective therapy with selected antibiotics in morbidly obese patients based on pharmacometric modelling and simulation


Morbidly obese patients represent a growing but understudied patient population with sparse and oftentimes contradicting information in the field of antiinfective therapy. To guarantee effective and safe treatment of bacterial infections targeted patient and pathogen specific antibiotic therapy is crucial.

For a pathogen specific therapy not only the antimicrobial spectrum of the drug but also adequacy of the dosing regimen and the resulting effective drug concentration-time profile at the target site are important. To measure effective drug concentration at target site, in case of antiinfectives mostly the extracellular interstitial fluid in the tissue, the minimally invasive method microdialysis is selected.

Regarding patient specific antibiotic therapy the oftentimes significant variability of the drug concentration-time profile is of interest. Employing standard dosing regimens bears the risk of subtherapeutic or toxic concentration-time profiles which might lead to therapy failure, increased occurrence of adverse events and development of bacterial resistance.

My dissertation project includes the pharmacometric analysis of frequently employed antibiotics after standard dosing regimens in morbidly obese patients. Based on clinical data on plasma and target site drug concentrations using microdialysis nonlinear mixed effects models will be developed. In a first step the pharmacokinetics of the antibiotics will be characterised, i.e. the population specific concentration-time profile and the different levels of variability between and within patients. In a second step patient specific covariates (e.g. markers of organ function or disease status) will be identified, which explain inter- and intraindividual variability and their impact on model parameters will be quantified.

Simulations based on the developed model can be utilised to evaluate dosing regimens regarding achieving effective pathogen specific drug concentration-time profiles (pharmacodynamics/pharmacokinetic targets) in morbidly obese patients. If need be alternative adequate dosing regimen with reduced adverse events can be suggested. In such an integrative manor knowledge spanning different classes of antibiotics shall be generated. Additionally the aim is to guarantee antiinfective therapy success in this patient population and to reduce the development of further antibiotic resistance.