name	title	fuMetaTitel	body	fuDCcreator	fuDCcreatorLinks	fuDCpublisher	fuDCpublisherLocation	fuDCSizeOrDuration	fuDCdateYear	fuDCdateMonth	fuDCdateDay	fuDCsubject	fuDCrelationPartOf	fuDCrelation	fuDClanguage	fuDCsource	fuDCcontributor	fuDCtype	fuDCformat	fuDCidentifier	fuDCcoverage	fuDCrights	fuDCdescriptionDE	fuDCdescriptionEN	fuDCIdentifierBibliographicCitation	fuBild	fuDCBibtex	fuLinksZumThema
KA970	Development and characterization of polyclonal peptide antibodies for the detection of Yellow fever virus proteins			"Stock, N. K.; Escadafal, C.; Achazi, K.; Cisse, M.; Niedrig, M."		J Virol Methods			2015	9	15	"Animals; Antibodies, Viral/immunology/isolation & purification/*metabolism; Antigens, Viral/*analysis/immunology/metabolism; Blotting, Western; Capsid protein; Capsid Proteins/*analysis/immunology/metabolism; Cell Nucleus/chemistry/virology; Chlorocebus aethiops; Cross Reactions; Diagnostic Tests, Routine/methods; Fluorescent Antibody Technique; Guinea Pigs; NS1 protein; Peptide antisera; Peptides/isolation & purification/*metabolism; Rabbits; Sensitivity and Specificity; Vero Cells; Viral Nonstructural Proteins/*analysis/immunology/metabolism; Virology/methods; Yellow fever virus; Yellow fever virus/immunology/*isolation & purification"				https://doi.org/10.1016/j.jviromet.2015.06.006		journalArticle		1879-0984 (Electronic) 0166-0934 (Linking)				There is still a considerable need for development of new tools and methods detecting specific viral proteins for the diagnosis and pathogenesis study of the Yellow fever virus (YFV). This study aimed to develop and characterize polyclonal peptide antisera for detection of YFV-C and -NS1 proteins. The antisera were used further to investigate NS1 protein expression during YFV infection in mammalian cells. YFV target proteins were detected by all antisera in western blot and immunofluorescence assays. No cross-reactivity was observed with Dengue virus, West Nile virus, Tick-borne encephalitis virus and Japanese encephalitis virus. Nuclear localization of the YFV-C protein was demonstrated for the first time. Experiments investigating NS1 expression suggested a potential use of the YFV-NS1 antisera for development of diagnostic approaches targeting the secreted form of the NS1 protein. The antisera described in this study offer new possibilities for use in YFV research and for the development of novel diagnostic tests.				
KA971	Supramolecular hydrophobic guest transport system based on pillar[5]arene			"Qi, Z.; Achazi, K.; Haag, R.; Dong, S.; Schalley, C. A."		Chem Commun (Camb)			2015	6	28	"Carbolines/*chemistry/toxicity; Drug Carriers/*chemistry; HEK293 Cells; Hep G2 Cells; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Macrocyclic Compounds/*chemistry; Monocytes; Proton Magnetic Resonance Spectroscopy; Solubility; Spectrometry, Fluorescence"				https://doi.org/10.1039/c5cc03955j		journalArticle		1364-548X (Electronic) 1359-7345 (Linking)				A pillar[5]arene-based bioactive guest loading system has been developed, which can increase the solubility of the drug norharmane in aqueous medium, and also enable its pH-stimulated release. Furthermore, this supramolecular transport system reduces the toxicity of loaded guest.				
KA972	Carbon-based cores with polyglycerol shells - the importance of core flexibility for encapsulation of hydrophobic guests			"Lukowiak, M. C.; Ziem, B.; Achazi, K.; Gunkel-Grabole, G.; Popeney, C. S.; Thota, B. N. S.; Bottcher, C.; Krueger, A.; Guan, Z.; Haag, R."		Journal of Materials Chemistry B			2015	2	7	"agents; architectures; chain-walking; drug-delivery systems; nanocarriers; nanoparticles; polymer therapeutics; release; solubilization; unimolecular micelles"				https://doi.org/10.1039/c4tb01858c		journalArticle		2050-7518 (Electronic) 2050-750X (Linking)				Two core-shell nanoparticles with polyglycerol shells and sp(3) carbon cores with different flexibilities (soft dendritic polyethylene and hard nanodiamond) were synthesized, their encapsulation capacities were compared, and their ability to transport into tumor cells was investigated. The nanocarrier with a soft core was superior to the hard one.				
KA973	Dendronized Multifunctional Amphiphilic Polymers as Efficient Nanocarriers for Biomedical Applications			"Kumari, M.; Gupta, S.; Achazi, K.; B ttcher, C.; Khandare, J.; Sharma, S. K.; Haag, R."		Macromolecular rapid communications			2015	1		"aliphatic polyesters; amphiphilic dendronized polymers; biological significance; block-copolymer micelles; catalyzed synthesis; click chemistry; dendritic amphiphiles; drug-delivery; hyperbranched polyglycerol; hyperbranched polyglycerols; lipase-b; novozym 435; solvent; unimolecular micelles"				https://doi.org/10.1002/marc.201400467		journalArticle		1022-1336				"To gain insight into the factors that affect stability and transport efficiency under dilution conditions, dendronized and hyperbranched multifunctional amphiphilic polymers are synthesized by following the ""grafting to"" approach using varied amounts of propargylated alkyl chain with perfect and hyperbranched polyglycerol dendrons on the base copolymer of PEG (($) over bar (n): 1000 g mol(-1)) diethylester and 2-azidopropane-1,3-diol following the ""bio-catalytic method"" and ""click approach"". The dendronized and hyperbranched polymeric systems form supramolecular aggregates and exhibit an efficient transport potential for the model dye ""Nile red"" in the low mu M range in the core-shell-type architecture provided with distinct amphiphilicity as required for encapsulation. Cytotoxicity studies show the polymeric systems to be non-toxic over a wide concentration range. The cellular internalization of Nile-red-encapsulated supramolecular micellar structures is also studied using cellular fluorescence microscopy and fluorescence-activated cell sorting (FACS) measurements. A comparison of the data for the dendronized polymers (PEG (M) over bar (n): 600/1000 g mol(-1)) with the respective low-molecular-weight amphiphile reveal that these polymeric systems are excellent nanotransporters."				
KA974	Encapsulation and cellular internalization of cyanine dye using amphiphilic dendronized polymers			"Kumar, S.; Achazi, K.; B ttcher, C.; Licha, K.; Haag, R.; Sharma, S. K."		European Polymer Journal			2015	8		"aliphatic polyesters; azidotriglycerol; catalyzed synthesis; cyanine 3; dendrimers; dendronized polymer; drug-delivery; glycerol; indocyanine green; nanocarriers; novozym 435; polyethylene glycol; solvent; systems; unimolecular micelles"				https://doi.org/10.1016/j.eurpolymj.2015.06.017		journalArticle		0014-3057				An amphiphilic molecular transporter has been developed by immobilized Candida antarctica lipase catalyzed copolymerization of PEG diethyl ester and azidotriglycerol via its primary hydroxyl groups. The co-polymer was further grafted by the C-12 alkyl chain and polyglycerol dendron moieties via the azide group of the polymer backbone by following 'Click' chemistry approach. The secondary hydroxyl groups on the backbone provide an additional site for acylation and fine-tune the physico-chemical properties. The aggregation behavior and transport potential of the resulting amphiphilic polymers were studied by surface tension (pendant drop method), DLS, cryo-TEM, UV and fluorescence measurements. The particle size of the micelles was found to have an average diameter of 8-12 nm. The encapsulation potential of these polymeric systems using cyanine 3, a highly fluorescent dye, used frequently for cellular imaging, has been studied. The transport capacity was observed to be in the range 7.58-19.15 mmol/mol of polymer. The cytotoxic study of the polymeric materials carried by using adenocarcinoma human alveolar basal epithelial cell lines (A549) and doxorubicin as a control drug revealed that a fine balance between hydrophilic (polyglycerol moiety) and the hydrophobic (alkyl chain) forces is critical to maintain minimal cytotoxicity. The cellular uptake of Cy3 encapsulated polymers were also studied by means of confocal laser scanning fluorescence microscopy (CFM) and fluorescence activated cell sorting (FACS) measurements. Among all the polymers, [G2.0] grafted dendronized polymer 5c exhibit prominent transport potential. (C) 2015 Elsevier Ltd. All rights reserved.				
KA975	Systematic adjustment of charge densities and size of polyglycerol amines reduces cytotoxic effects and enhances cellular uptake			"Hellmund, M.; Achazi, K.; Neumann, F.; Thota, B. N.; Ma, N.; Haag, R."		Biomater Sci			2015	11		"Cations/*chemistry; DNA/*chemistry/genetics/metabolism; Gene Transfer Techniques; Genetic Vectors/*chemistry/drug effects; Glycerol/*chemistry; Imines/*chemistry; Polyamines/*chemistry; Polyethylene Glycols/*chemistry; Polyethyleneimine/*chemistry; Polyethylenes/*chemistry; Polymers/*chemistry"				https://doi.org/10.1039/c5bm00187k		journalArticle		2047-4849 (Electronic) 2047-4830 (Linking)				Excessive cationic charge density of polyplexes during cellular uptake is still a major hurdle in the field of non-viral gene delivery. The most efficient cationic vectors such as polyethylene imine (PEI) or polyamidoamine (PAMAM) can be highly toxic and may induce strong side effects due to their high cationic charge densities. Alternatives like polyethylene glycol (PEG) are used to 'shield' these charges and thus to reduce the cytotoxic effects known for PEI/PEG-core-shell architectures. In this study, we compared the ability of hyperbranched polyglycerol amines (hPG amines) with different amine densities and molecular weights as non-viral cationic vectors for DNA delivery. By adjusting the hydroxyl to amine group ratio on varying molecular weights, we were able to perform a systematic study on the cytotoxic effects caused by the effective charge density in correlation to size. We could demonstrate that carriers with moderate charge density have a higher potential for effective DNA delivery as compared to high/low charged ones independent of their size, but the final efficiency can be optimized by the molecular weight. We analyzed the physicochemical properties and cellular uptake capacity as well as the cytotoxicity and transfection efficiency of these new vector systems.				
KA976	Charge-conversional and reduction-sensitive poly(vinyl alcohol) nanogels for enhanced cell uptake and efficient intracellular doxorubicin release			"Chen, W.; Achazi, K.; Schade, B.; Haag, R."		Journal of controlled release : official journal of the Controlled Release Society			2015	5	10	"*Drug Carriers; *Nanoparticles; Antibiotics, Antineoplastic/chemistry/*metabolism/pharmacology; Biological Transport; Breast Neoplasms/drug therapy/*metabolism/pathology; Cell Survival/drug effects; Charge-conversion; Chemistry, Pharmaceutical; Click Chemistry; Delayed-Action Preparations; Dose-Response Relationship, Drug; Doxorubicin/chemistry/*metabolism/pharmacology; Drug delivery; Female; Gels; Humans; Hydrogen-Ion Concentration; Inhibitory Concentration 50; Inverse nanoprecipitation; MCF-7 Cells; Microscopy, Confocal; Nanomedicine; Oxidation-Reduction; Particle Size; Polyvinyl Alcohol/analogs & derivatives/*chemistry; PVA nanogel; Solubility; Stimuli-responsive; Surface Properties; Technology, Pharmaceutical/methods; Time Factors"				https://doi.org/10.1016/j.jconrel.2014.11.012		journalArticle		1873-4995 (Electronic) 0168-3659 (Linking)				"Charge-conversional and reduction-sensitive polyvinyl alcohol (PVA) nanogels were developed for efficient cancer treatment by enhanced cell uptake and intracellular triggered doxorubicin (DOX) release. These PVA nanogels were prepared in a straightforward manner by inverse nanoprecipitation via ""click"" reaction with an average diameter of 118nm. The introduction of COOH into the PVA nanogels efficiently improved the DOX encapsulation due to the electrostatic interaction. The in vitro release result showed that the decrease of electrostatic interaction between COOH and DOX under a mimicking endosomal pH, in combination with the cleavage of the intervening disulfide bonds in response to a high glutathione (GSH) concentration led to a fast and complete release of DOX. Furthermore, confocal laser scanning microscopy (CLSM) revealed that the ultra pH-sensitive terminal groups allowed nanogels to reverse their surface charge from negative to positive under a tumor extracellular pH (6.5-6.8) which facilitated cell internalization. MTT assays and real time cell analysis (RTCA) showed that these DOX-loaded charge-conversional and reducible PVA nanogels had much better cell toxicity than DOX-loaded non-charge-conversional or reduction-insensitive PVA nanogels following 48h of incubation. These novel charge-conversional and stimuli-responsive PVA nanogels are highly promising for targeted intracellular anticancer drug release."				
KA977	Tumor-pH activated charge-conversional and reducible poly(vinyl alcohol) nanogels for enhanced cell uptake and intracellular DOX release			"Chen, W.; Achazi, K.; Haag, R."		J Control Release			2015	9	10	"charge-conversion; intracellular release; nanogel; poly(vinyl alcohol); reduction-sensitive"				https://doi.org/10.1016/j.jconrel.2015.05.181		journalArticle		1873-4995 (Electronic) 0168-3659 (Linking)								
KA978	Engineering thermoresponsive polyether-based nanogels for temperature dependent skin penetration			"Asadian-Birjand, M.; Bergueiro, J.; Rancan, F.; Cuggino, J. C.; Mutihac, R. C.; Achazi, K.; Dernedde, J.; Blume-Peytayi, U.; Vogt, A.; Calder n, M."		Polymer Chemistry			2015			"barrier; delivery; nanoparticles; particles; polymers; release"				https://doi.org/10.1039/c5py00924c		journalArticle		1759-9954				Highly biocompatible thermoresponsive nanogels (tNGs) based on oligo ethylene glycol (OEG) as thermoresponsive unit and dendritic polyglycerol (dPG) as cross-linker, were precisely engineered in terms of size and volume phase transition temperature (VPTT). Preliminary uptake studies into human skin were realized to show the temperature-dependent internalization behavior of these systems.				
KA979	A facile approach for dual-responsive prodrug nanogels based on dendritic polyglycerols with minimal leaching			"Zhang, X.; Achazi, K.; Steinhilber, D.; Kratz, F.; Dernedde, J.; Haag, R."		Journal of controlled release : official journal of the Controlled Release Society			2014	1	28	"Antibiotics, Antineoplastic/administration & dosage/chemistry; Cell Survival/drug effects; Cellular uptake; Controlled drug release; Doxorubicin/administration & dosage/chemistry; Drug Compounding/*methods; Gels/administration & dosage/*chemistry; Glycerol/chemistry; HeLa Cells; Humans; Nanogel degradation; Nanostructures/administration & dosage/*chemistry; Polyglycerol nanogel; Polymers/chemistry; Prodrug; Prodrugs/administration & dosage/*chemistry"				https://doi.org/10.1016/j.jconrel.2013.11.005		journalArticle		1873-4995 (Electronic) 0168-3659 (Linking)				A novel pH and redox dual-responsive prodrug nanogel was prepared by an inverse nanoprecipitation method, which is mild and surfactant free, and based on a thiol-disulfide exchange reaction and thiol-Michael addition reaction. Highly biocompatible hyperbranched polyglycerol (hPG) was cross-linked with disulfide bonds, to obtain biodegradable nanogels, which could be degraded under intracellular reductive conditions. Doxorubicin (DOX) was conjugated to the biodegradable nanogel matrix via an acid-labile hydrazone linker. This is the first dual-responsive prodrug nanogel system that shows very low unspecific drug leaching, but efficient intracellular release of the payload triggered by the intracellular conditions. Two different prodrug nanogels were prepared with a size of approximately 150nm, which is big enough to take the advantage of the enhanced permeation and retention (EPR) effect in tumor tissue. Cell culture analysis by microscopy and flow cytometry revealed that the prodrug nanogels were efficiently internalized by tumor cells. Distinct release profiles of DOX were achieved by adjusting the nanogel architecture, and online detection of cytotoxicity showed that, unlike free DOX, the dual-responsive prodrug nanogels exhibited a delay in the onset of toxicity, indicating the different uptake mechanism and the need for prodrug activation to induce cell death.