Advanced light microscopy and cell-based assays in biomedical research and neuroscience (Seminar/Lab Course) Katharina Achazi, Marta Maglione (in total 4 SWS, VLVZ no.: 216624 a/b). This block course is intended for students with an interest in biomedical and neurobiological issues relevant to research. In particular, the course introduces advanced light microscopy and other cell-based biochemical and molecular biological as well as analytical methods. In the first part of the methods course, the analysis of cells and cellular processes as well as basics for the cultivation of cells, sterile work, preparation of cells and tissues including fixation methods, staining techniques and dyes especially fluorescent dyes as well as safety relevant aspects when handling biological samples are covered theoretically and practically. The second part covers theoretical and practical aspects of various light microscopic methods including fluorescence, confocal and STED microscopy as well as imaging and image evaluation, digital image processing and image analysis as well as the design, function and handling of light microscopes.
Note: For Chemistry students there are 2 places each for chemistry students. Places will be allocated online via the WebApp. For Biochemistry students there are 4 places each for chemistry students. Places will be allocated online via the link on the summary of the available methods course modules on the information page for enrolled master students.