Tracking cell migration

Tracking cell migration

(top) Model of chemotaxis assay chamber containing observation area connected to two reservoirs used for generation of ligands/growth factor gradient (figure adapted from Ibidi). (bottom) Left: Phase contrast image of cells with annotated trajectories for migrating single cells. Right: Model of spatio-temporal migratory behavior by Markov committor analysis. All cells were tracked and analyzed by Markov State Model in response to growth factor gradient (gray triangle). Red line depicts border between up and downward migrating cells with light blue for upwards and darker blue areas for downward migrating cells.