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Immune gene regulation in the gut during metamorphosis in a holo- versus a hemimetabolous insect

rstb.2019.374.issue-1783.cover

rstb.2019.374.issue-1783.cover

Johnston, Paul R. and Paris, Véronique and Rolff, Jens – 2019

During metamorphosis, holometabolous insects completely replace the larval gut and must control the microbiota to avoid septicaemia. Rapid induction of bactericidal activity in the insect gut at the onset of pupation has been described in numerous orders of the Holometabola and is best-studied in the Lepidoptera where it is under control of the 20-hydroxyecdysone (20E) moulting pathway. Here, using RNAseq, we compare the expression of immune effector genes in the gut during metamorphosis in a holometabolous (Galleria mellonella) and a hemimetabolous insect (Gryllus bimaculatus). We find that in G. mellonella, the expression of numerous immune effectors and the transcription factor GmEts are upregulated, with peak expression of three antimicrobial peptides (AMPs) and a lysozyme coinciding with delamination of the larval gut. By contrast, no such upregulation was detectable in the hemimetabolous Gr. bimaculatus. These findings support the idea that the upregulation of immune effectors at the onset of complete metamorphosis is an adaptive response, which controls the microbiota during gut replacement. This article is part of the theme issue ‘The evolution of complete metamorphosis’.

Titel
Immune gene regulation in the gut during metamorphosis in a holo- versus a hemimetabolous insect
Verfasser
Johnston, Paul R. and Paris, Véronique and Rolff, Jens
Verlag
Philosophical Transactions of the Royal Society B: Biological Sciences
Datum
2019
Kennung
https://doi.org/10.1098/rstb.2019.0073
Sprache
eng
Art
Text
BibTeX Code
@article{doi:10.1098/rstb.2019.0073,
author = {Johnston, Paul R. and Paris, Véronique and Rolff, Jens },
title = {Immune gene regulation in the gut during metamorphosis in a holo- versus a hemimetabolous insect},
journal = {Philosophical Transactions of the Royal Society B: Biological Sciences},
volume = {374},
number = {1783},
pages = {20190073},
year = {2019},
doi = {10.1098/rstb.2019.0073},

URL = {https://royalsocietypublishing.org/doi/abs/10.1098/rstb.2019.0073},
eprint = {https://royalsocietypublishing.org/doi/pdf/10.1098/rstb.2019.0073}
,
abstract = { During metamorphosis, holometabolous insects completely replace the larval gut and must control the microbiota to avoid septicaemia. Rapid induction of bactericidal activity in the insect gut at the onset of pupation has been described in numerous orders of the Holometabola and is best-studied in the Lepidoptera where it is under control of the 20-hydroxyecdysone (20E) moulting pathway. Here, using RNAseq, we compare the expression of immune effector genes in the gut during metamorphosis in a holometabolous (Galleria mellonella) and a hemimetabolous insect (Gryllus bimaculatus). We find that in G. mellonella, the expression of numerous immune effectors and the transcription factor GmEts are upregulated, with peak expression of three antimicrobial peptides (AMPs) and a lysozyme coinciding with delamination of the larval gut. By contrast, no such upregulation was detectable in the hemimetabolous Gr. bimaculatus. These findings support the idea that the upregulation of immune effectors at the onset of complete metamorphosis is an adaptive response, which controls the microbiota during gut replacement. This article is part of the theme issue ‘The evolution of complete metamorphosis’. }
}