Institut_Pharmazie

Dr. rer. nat. Alexander Weng

Weng

Institut für Pharmazie

Wissenschaftlicher Mitarbeiter

Aufgabenbereich

Pharmazeutische Biologie

Adresse Freie Universität Berlin
Institut für Pharmazie
Königin-Luise-Str. 2+4
Raum 470/471
14195 Berlin
Telefon 030 838 51265
Fax 030 838 51265
E-Mail alexander.weng@fu-berlin.de
  • Isolation und characterization of natural products with special focus on triterpene saponins und ribosome-inactivating proteins
  • Development of siRNA- and DNA-nanocarrier
  • Tools to improve the endosomal escape process
  • Targeted toxins (Immunotoxins)
  • Weng A, Manunta MD, Thakur M, Gilabert-Oriol R, Tagalakis AD, Eddaoudi A, et al. (2015). Improved intracellular delivery of peptide- and lipid-nanoplexes by natural glycosides. Journal of controlled release : official journal of the Controlled Release Society.
  • Holmes SE, Bachran C, Fuchs H, Weng A, Melzig MF, Flavell SU, et al. (2015). Triterpenoid saponin augmention of saporin-based immunotoxin cytotoxicity for human leukaemia and lymphoma cells is partially immunospecific and target molecule dependent. Immunopharmacology and immunotoxicology 37: 42-55.
  • Thakur M, Jerz G, Tuwalska D, Gilabert-Oriol R, Wybraniec S, Winterhalter P, et al. (2014). High-speed countercurrent chromatographic recovery and off-line electrospray ionization mass spectrometry profiling of bisdesmodic saponins from Saponaria officinalis possessing synergistic toxicity enhancing properties on targeted antitumor toxins. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 955-956: 1-9.
  • von Mallinckrodt B, Thakur M, Weng A, Gilabert-Oriol R, Durkop H, Brenner W, et al. (2014). Dianthin-EGF is an effective tumor targeted toxin in combination with saponins in a xenograft model for colon carcinoma. Future oncology 10: 2161-2175.
  • Gilabert-Oriol R, Weng A, Mallinckrodt B, Melzig MF, Fuchs H, Thakur M (2014). Immunotoxins constructed with ribosome-inactivating proteins and their enhancers: a lethal cocktail with tumor specific efficacy. Current pharmaceutical design 20: 6584-6643.
  • Gilabert-Oriol R, Thakur M, von Mallinckrodt B, Bhargava C, Wiesner B, Eichhorst J, et al. (2014). Reporter assay for endo/lysosomal escape of toxin-based therapeutics. Toxins 6: 1644-1666.
  • Gilabert-Oriol R, Thakur M, von Mallinckrodt B, Hug T, Wiesner B, Eichhorst J, et al. (2013). Modified trastuzumab and cetuximab mediate efficient toxin delivery while retaining antibodydependent cell-mediated cytotoxicity in target cells. Molecular pharmaceutics 10: 4347-4357.
  • Gilabert-Oriol R, Mergel K, Thakur M, von Mallinckrodt B, Melzig MF, Fuchs H, et al. (2013). Real-time analysis of membrane permeabilizing effects of oleanane saponins. Bioorganic & medicinal chemistry 21: 2387-2395.
  • Gilabert-Oriol R, Thakur M, Weise C, Dernedde J, von Mallinckrodt B, Fuchs H, et al. (2013). Small structural differences of targeted anti-tumor toxins result in strong variation of protein expression. Protein expression and purification 91: 54-60.
  • Thakur M, Mergel K, Weng A, von Mallinckrodt B, Gilabert-Oriol R, Durkop H, et al. (2013). Targeted tumor therapy by epidermal growth factor appended toxin and purified saponin: an evaluation of toxicity and therapeutic potential in syngeneic tumor bearing mice. Molecular oncology 7: 475-483.
  • Thakur M, Weng A, Pieper A, Mergel K, von Mallinckrodt B, Gilabert-Oriol R, et al. (2013). Macromolecular interactions of triterpenoids and targeted toxins: role of saponins charge. International journal of biological macromolecules 61: 285-294.
  • Voutquenne-Nazabadioko L, Gevrenova R, Borie N, Harakat D, Sayagh C, Weng A, et al. (2013). Triterpenoid saponins from the roots of Gypsophila trichotoma Wender. Phytochemistry 90: 114-127.
  • Weng A, Thakur M, von Mallinckrodt B, Beceren-Braun F, Gilabert-Oriol R, Wiesner B, et al. (2012). Saponins modulate the intracellular trafficking of protein toxins. Journal of controlled release : official journal of the Controlled Release Society 164: 74-86.
  • Weng A, Thakur M, Beceren-Braun F, Bachran D, Bachran C, Riese SB, et al. (2012). The toxin component of targeted anti-tumor toxins determines their efficacy increase by saponins. Molecular oncology 6: 323-332.
  • Thakur M, Mergel K, Weng A, Frech S, Gilabert-Oriol R, Bachran D, et al. (2012). Real time monitoring of the cell viability during treatment with tumor-targeted toxins and saponins using impedance measurement. Biosensors & bioelectronics 35: 503-506.
  • Thakur M, Weng A, Bachran D, Riese SB, Bottger S, Melzig MF, et al. (2011). Electrophoretic isolation of saponin fractions from Saponinum album and their evaluation in synergistically enhancing the receptor-specific cytotoxicity of targeted toxins. Electrophoresis 32: 3085-3089.
  • Bachran D, Schneider S, Bachran C, Weng A, Melzig MF, Fuchs H (2011). The endocytic uptake pathways of targeted toxins are influenced by synergistically acting Gypsophila saponins. Molecular pharmaceutics 8: 2262-2272.
  • Weng A, Thakur M, Schindler A, Fuchs H, Melzig MF (2011). Liquid-chromatographic profiling of Saponinum album (Merck). Die Pharmazie 66: 744-746.
  • Bachran D, Schneider S, Bachran C, Urban R, Weng A, Melzig MF, et al. (2010). Epidermal growth factor receptor expression affects the efficacy of the combined application of saponin and a targeted toxin on human cervical carcinoma cells. International journal of cancer. Journal international du cancer 127: 1453-1461.
  • Weng A, Jenett-Siems K, Schmieder P, Bachran D, Bachran C, Gorick C, et al. (2010). A convenient method for saponin isolation in tumour therapy. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 878: 713-718.
  • Bachran C, Weng A, Bachran D, Riese SB, Schellmann N, Melzig MF, et al. (2010). The distribution of saponins in vivo affects their synergy with chimeric toxins against tumours expressing human epidermal growth factor receptors in mice. British journal of pharmacology 159: 345-352.
  • Weng A, Bachran C, Fuchs H, Krause E, Stephanowitz H, Melzig MF (2009). Enhancement of saporin cytotoxicity by Gypsophila saponins--more than stimulation of endocytosis. Chemico-biological interactions 181: 424-429.
  • Weng A, Bachran D, Gorick C, Bachran C, Fuchs H, Melzig MF (2009). A simple method for isolation of Gypsophila saponins for the combined application of targeted toxins and saponins in tumor therapy. Planta medica 75: 1421-1422.
  • Bachran C, Durkop H, Sutherland M, Bachran D, Muller C, Weng A, et al. (2009). Inhibition of tumor growth by targeted toxins in mice is dramatically improved by saponinum album in a synergistic way. Journal of immunotherapy 32: 713-725.
  • Fuchs H, Bachran D, Panjideh H, Schellmann N, Weng A, Melzig MF, et al. (2009). Saponins as tool for improved targeted tumor therapies. Current drug targets 10: 140-151.
  • Weng A, Gorick C, Melzig MF (2009). Enhancement of toxicity of saporin-based toxins by Gypsophila saponins--kinetic of the saponin. Experimental biology and medicine 234: 961-966.
  • Weng A, Jenett-Siems K, Gorick C, Melzig MF (2008). Enhancement of cytotoxicity of ribosome-inactivating-protein type I by saponinum album is not based on stimulation of phagocytosis. The Journal of pharmacy and pharmacology 60: 925-930.
  • Weng A, Melzig MF, Bachran C, Fuchs H (2008). Enhancement of saporin toxicity against U937 cells by Gypsophila saponins. Journal of immunotoxicology 5: 287-292.
  • Hebestreit P, Weng A, Bachran C, Fuchs H, Melzig MF (2006). Enhancement of cytotoxicity of lectins by Saponinum album. Toxicon : official journal of the International Society on Toxinology 47: 330-335.