Confocal laser scanning microscopy enables optical sectioning of multilayer fluorescent specimens with high contrast by applying a pinhole in the optics. It is therefore possible to image a thin optical slice out of a thick specimen (up to 100 µm) that represents under optimal conditions a slice of approximately 500 nm. Moreover, conventional contrast methods such as the differential interference contrast (DIC) can be used and in reflection mode particles or surfaces can be analyzed.
The CLSM SP8 (1) based on a DMI6000CSB allows precise detection of standard fluorophores in your sample. The system excites via diode laser at 405 nm, Argonlaser combined with AOBS at 458, 488, 514 nm, diode laser at 561 nm and a He/Ne laser at 633 nm. The SP8 (1) has two standard GaAsp photomultipliers (PMTs) and two extremely sensitive Hybrid detectors (HyD), combining PMT and avalanche photodiode (APD) technology. An optional incubation chamber allows imaging of living cells.
The system is almost fully automated and offers with its broad excitation spectra, its automated system parts, the galvo stage and the optional incubation chamber a variety of possible uses.
Immersion objectives are optimized for oil- / glycerol immersion.
Detailed specifications CLSM Leica SP8 (1)
Conventional fluorescence filters for eyepiece vizualization:
HP Z420 Workstation, Windows 7-64 bit, LAS X