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Instruments

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Quantitative Proteomics

  • Metabolic stable isotopic labeling (SILAC)
  • Enzymatic stable isotopic labeling (18O)
  • Label-free quantification

Protein-Protein Interactions (PPIs)

  • Identification & Quantification of unknown binding partners after enrichment of protein complexes by pulldown experiments or coIP (Affinity-Purification MS)

Protein Identification

  • Quality control of purified proteins including the analysis of post-translational modifications (PTMs)
  • Determination of intact protein masses of purified proteins and protein complexes by classical MS and native MS
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Protein identification

  • Protein identification via peptide mass fingerprint after trypsin digestion in gel and peptide sequencing by a MASCOT database search

Protein characterization

  • Identification of common protein modifications (phosphorylation, acetylation) and
    N- and C-terminal modifications

Protein profiling

  • Analysis of proteins from complex mixtures by SELDI-chip technology
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