Glycan analysis by ion mobility-mass spectrometry and gas-phase spectroscopy

Manz, C.; Pagel, K.*— 2018

Due to the existence of numerous isomers, the in-depth analysis of glycans represents a major challenge. Currently, the majority of glycans are analysed using mass spectrometry (MS)-based techniques, which can provide information on regioisomers but usually fail to differentiate stereoisomers. A promising approach to overcome this limitation is to implement ion mobility spectrometry (IMS) as an additional gas-phase separation dimension. This review highlights recent developments in which IM-MS was used as a tool for comprehensive glycan analysis or as rapid screening method for glycan feature analysis. Furthermore, we summarize a series of very recent investigations in which gas-phase spectroscopy is applied to study glycans and discuss the potential of the hyphenation between IM-MS and infrared (IR) spectroscopy as a future tool for glycomics and glycoproteomics.

TitleGlycan analysis by ion mobility-mass spectrometry and gas-phase spectroscopy
AuthorManz, C.; Pagel, K.*
Date2018
Identifier10.1016/j.cbpa.2017.10.021
Source(s)
CitationCurr. Opin. Chem. Biol. 2018, 42, 16-24
TypeText