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Global N-glycan Site Occupancy of HIV-1 gp120 by High-Resolution Intact Mass Spectrometry

Struwe, W.*; Stuckmann, A.; Behrens, A.-J.; Pagel, K.; Crispin, M.* – 2016

A vital step in HIV vaccine development strategies has been the observation that some infected individuals generate broadly neutralizing antibodies that target the glycans on the surface of HIV-1 gp120. These antibodies target glycan epitopes on viral envelope spikes and yet the positions and degree of occupancy of glycosylation sites is diverse. Therefore, there is a need to understand glycosylation occupancy on recombinant immunogens. The sheer number of potential glycosylation sites and degree of chemical heterogeneity impedes assessing the global sequon occupancy of gp120 glycoforms. Here, we trap the glycan processing of recombinant gp120 to generate homogenous glycoforms, facilitating occupancy assessment by intact mass spectrometry. We show that gp120 monomers of the BG505 strain contain either fully occupied sequenz or missing one and sometimes two glycans across the molecule. This biosynthetic engineering approach enables the analysis of therapeutically important glycoproteins otherwise recalcitrant to analysis by native mass spectrometry.

Title
Global N-glycan Site Occupancy of HIV-1 gp120 by High-Resolution Intact Mass Spectrometry
Author
Struwe, W.*; Stuckmann, A.; Behrens, A.-J.; Pagel, K.; Crispin, M.*
Date
2016
Identifier
10.1021/acschembio.6b00854
Source(s)
Citation
ACS Chem. Biol. 2017, 12, 357-361
Type
Text
masses4masses
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