Distinguishing N-Acetylneuraminic Acid Linkage Isomers on Glycopeptides by Ion Mobility-Mass Spectrometry

Hinneburg, H.; Hofmann, J.; Struwe, W. B.; Thader, A.; Altmann, F.; Varón Silva, D.; Seeberger, P.; Pagel, K.*; Kolarich, D.*— 2016

Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions.

TitleDistinguishing N-Acetylneuraminic Acid Linkage Isomers on Glycopeptides by Ion Mobility-Mass Spectrometry
AuthorHinneburg, H.; Hofmann, J.; Struwe, W. B.; Thader, A.; Altmann, F.; Varón Silva, D.; Seeberger, P.; Pagel, K.*; Kolarich, D.*
Date2016
Identifier10.1039/C6CC01114D
Source(s)
CitationChem. Commun. 2016, 52, 4381-4384
TypeText