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31. MicroRNA Detection by DNA-Mediated Liposome Fusion Using a 3D Single Vesicle Tracking FRET Assay

Jumeaux, C.; Wahlsten, O.; Block, S.; Kim, E.; Chandrawati, R.; Howes, P.D.; Höök, F.*; Stevens, M.M.* – 2018

Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal-generating molecules. In this work, sequence-specific DNA-mediated liposome fusion is used for the highly selective detection of microRNA. The detection of miR-29a, a known flu biomarker, is demonstrated down to 18 nm within 30 min with high specificity by using a standard laboratory microplate reader. Furthermore, one order of magnitude improvement in the limit of detection is demonstrated by using a novel imaging technique combined with an intensity fluctuation analysis, which is coined two-color fluorescence correlation microscopy.

Title
31. MicroRNA Detection by DNA-Mediated Liposome Fusion Using a 3D Single Vesicle Tracking FRET Assay
Author
Jumeaux, C.; Wahlsten, O.; Block, S.; Kim, E.; Chandrawati, R.; Howes, P.D.; Höök, F.*; Stevens, M.M.*
Date
2018
Identifier
10.1002/cbic.201700592
Citation
ChemBioChem 2018, 19, 434-438