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Dr. Lisa von Kleist

Lisa studied chemistry with emphasis on biochemistry at Göteborg University where she obtained her M.Sc. in 2003. She performed her M.Sc. thesis work at AstraZeneca R&D focusing on the pharmacological characterization of metabotropic glutamate receptors. She continued at AstraZeneca as a scientist in the ion channel group where she contributed to research projects involving the biology and pharmacology of ion channels and their role in disease.

In 2006, she started a Ph.D. with Prof. Volker Haucke working on clathrin mediated endocytosis. During her Ph.D., she identified novel small molecule inhibitors (termed pitstopsTM) that selectively interfere with clathrin binding. The work was published in Cell in 2011.

After her Ph.D., Lisa started as a senior scientist at caprotec bioanalytics GmbH to work on target identification of small molecules using capture compound mass spectrometry (CCMS). She joined the group of Dr. Tobias Stauber as a Postdoc in 2015 where her main research interest is to elucidate mechanisms of calcium homeostasis in mammalian cells.

Lisa

Biochemistry

Postdoc

Address Thielallee 63
Room 129
14195 Berlin
Telephone +49 30 838-70988
Email lisa.kleist@fu-berlin.de
  • von Kleist L, Stahlschmidt W, Bulut H, Gromova K, Puchkov D, Robertson MJ, MacGregor KA, Tomilin N, Pechstein A, Chau N, Chircop M, Sakoff J, von Kries JP, Saenger W, Kräusslich HG, Shupliakov O, Robinson PJ, McCluskey A, Haucke V. Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition. Cell, 146(3), 471–484, 2011
  • von Kleist L, Haucke V. At the crossroads of chemistry and cell biology: inhibiting membrane traffic by small molecules. Traffic, 13(4), 495–504, 2012, Review
  • Robertson MJ, Hadzic G, Ambrus J, Pomè DY, Hyde E, Whiting A, Mariana A, von Kleist L, Chau N, Haucke V, Robinson PJ, McCluskey A. The rhodadyns, a new class of small molecule inhibitors of dynamin GTPase activity. ACS Med Chem Lett, 3(5), 352–356, 2012
  • McCluskey A, Daniel JA, Hadzic G, Chau N, Clayton EL, Mariana A, Whiting A, Gorgani NN, Lloyd J, Quan A, Moshkanbaryans L, Krishnan S, Perera S, Chircop M, von Kleist L, McGeachie AB, Howes MT, Parton RG, Campbell M, Sakoff JA, Wang X, Sun JY, Robertson MJ, Deane FM, Nguyen TH, Meunier FA, Cousin MA, Robinson PJ. Building a better dynasore: the dyngo compounds potently inhibit dynamin and endocytosis. Traffic,14(12), 1272–1289, 2013
  • Leclercq O*, Bartho K*, Duelsner E*, von Kleist L*, Gherardini PF, Palmeri A, Helmer-Citterich M, Baumgart S, Späth GF. Enrichment of Leishmania donovani ATP-binding proteins using a staurosporine capture compound. J Proteomics, 86, 97–104, 2013, *contributed equally
  • MacGregor KA, Robertson MJ, Young KA, von Kleist L, Stahlschmidt W, Whiting A, Chau N, Robinson PJ, Haucke V, McCluskey A. Development of 1,8-naphthalimides as clathrin inhibitors. J Med Chem, 57(1), 131–143, 2014
  • Robertson MJ, Deane FM, Stahlschmidt W, von Kleist L, Haucke V, Robinson PJ, McCluskey A. Synthesis of the pitstop family of clathrin inhibitors. Nature Protocols, 9(7), 1592-1606, 2014
  • Daniel JA, Chau N, Abdel-Hamid MK, Hu L, von Kleist L, Whiting A, Krishnan S, Maamary P, Joseph SR, Simpson F, Haucke V, McCluskey A, Robinson PJ. Phenothiazine-derived antipsychotic drugs inhibit dynamin and clathrin-mediated endocytosis.Traffic, 16(6), 635-654, 2015
  • Diezmann F, von Kleist L, Haucke V, Seitz O.  Probing heterobivalent binding to the endocytic AP-2 adaptor complex by DNA-based spatial screening. Organic Biomol Chem, 13(29), 8008-8015, 2015
  • Luo L, Xue J, Kwan A, Gamsjaeger R, Wielens J, von Kleist L, Cubeddu L, Guo Z, Stow JL, Parker MW, Mackay JP, Robinson PJ. The binding of syndapin SH3 domain to dynamin proline-rich domain involves short and long distance elements. J Biol Chem, 291(18), 9411–9424, 2016

  • von Kleist L, Michaelis S, Bartho K, Graebner O, Schlief M, Dreger M, Schrey AK, Sefkow M, Kroll F, Koester H, Luo Y. Identification of potential off-target toxicity liabilities of catechol-O-methyltransferase inhibitors by differential competition capture compound mass spectrometry. J Med Chem, 59(10), 4664–4675, 2016