giant fiber A (gfA) encodes a nicotinic acetylcholine receptor that mediates synaptic transmission in the giant fiber circuit of Drosophila

Fayyazuddin, A., Zaheer, M., Hiesinger, P.R., and Bellen, H.J.— 2006

Acetylcholine is the major excitatory neurotransmitter in the central nervous system of insects. Mutant analysis of the Dalpha7 nicotinic acetylcholine receptor (nAChR) of Drosophila shows that it is required for the giant fiber-mediated escape behavior. The Dalpha7 protein is enriched in the dendrites of the giant fiber, and electrophysiological analysis of the giant fiber circuit showed that sensory input to the giant fiber is disrupted, as is transmission at an identified cholinergic synapse between the peripherally synapsing interneuron and the dorsal lateral muscle motor neuron. Moreover, we found that gfA1, a mutation identified in a screen for giant fiber defects more than twenty years ago, is an allele of Dalpha7. Therefore, a combination of behavioral, electrophysiological, anatomical, and genetic data indicate an essential role for the Dalpha7 nAChR in giant fiber-mediated escape in Drosophila.

Titlegiant fiber A (gfA) encodes a nicotinic acetylcholine receptor that mediates synaptic transmission in the giant fiber circuit of Drosophila
AuthorFayyazuddin, A., Zaheer, M., Hiesinger, P.R., and Bellen, H.J.
PublisherPublic Library of Science
Date20060228
Identifierdoi: 10.1371/journal.pbio.0040063
Source(s)
Appeared InPloS Biology 4(3): e63
Languageeng
TypeText
Rights© 2006 Fayyazuddin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.