Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin.

jneuro99-cover
jneuro99-cover

Hiesinger, P.R., Reiter, C., Schau, H., and Fischbach, K.-F.— 1999

To investigate a possible involvement of synaptic machinery in Drosophila visual system development, we studied the effects of a loss of function of neuronal synaptobrevin, a protein required for synaptic vesicle release. Expression of tetanus toxin light chain (which cleaves neuronal synaptobrevin) and genetic mosaics were used to analyze neuropil pattern formation and levels of selected neural adhesion molecules in the optic lobe. We show that targeted toxin expression in the developing optic lobe results in disturbances of the columnar organization of visual neuropils and of photoreceptor terminal morphology. IrreC-rst immunoreactivity in neuropils is increased after widespread expression of toxin. In photoreceptors, targeted toxin expression results in increased Fasciclin II and chaoptin but not IrreC-rst immunoreactivity. Axonal pathfinding and programmed cell death are not affected. In genetic mosaics, patches of photoreceptors that lack neuronal synaptobrevin exhibit the same phenotypes observed after photoreceptor-specific toxin expression. Our results demonstrate the requirement of neuronal synaptobrevin for regulation of cell adhesion molecules and development of the fine structure of the optic lobe. A possible causal link to fine-tuning processes that may include synaptic plasticity in the development of the Drosophila CNS is discussed.

TitleNeuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin.
AuthorHiesinger, P.R., Reiter, C., Schau, H., and Fischbach, K.-F.
PublisherSociety for Neuroscience
SubjectDrosophila, optic lobe development, synaptobrevin, synaptic plasticity, cell adhesion molecules, tetanus toxin, Fasciclin II, IrreC-rst, chaoptin
Date19990901
Source(s)
Appeared InJ. Neurosci. 19(17): 7548-56
Languageeng
TypeText
Rights© 1999 Society for Neuroscience