Transfer of microsatellite primers and charakterisation of nuclear loci in congeneric species
Nuclear microsatellite loci are used for assessing genetic diversity within species. They thus play a major role in the analysis of populations of threatened species for the development of scientifically founded protection strategies. Here we will test the transferability of primers for microsatellites from other species within the same genus to a threatened species.
Proposal 1: Calligonum bakuense (Polygonaceae) is a shrub endemic to the Absheron-peninsula in Azerbaijan, where it is threatened by industrial activities.
Proposal 2: Gleditsia caspica (Fabaceae) is an endemic tree from the coastal region of the Caspian Sea (Iran, Azerbaijan) threatened by hybridization with a close relative from America.
- Methods: Plant DNA extraction, gel-electrophoresis, PCR, DNA-sequencing / fragment analysis, analysis of population genetic data