Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner

neuron2002-cover
neuron2002-cover

Pennetta, G., Hiesinger, P.R., Fabian-Fine, R., Meinertzhagen, I.A., and Bellen H.J.— 2002

Aplysia VAP-33 (VAMP-associated protein) has been previously proposed to be involved in the control of neurotransmitter release. Here, we show that a Drosophila homolog of VAP-33, DVAP-33A, is localized to neuromuscular junctions. Loss of DVAP-33A causes a severe decrease in the number of boutons and a corresponding increase in bouton size. Conversely, presynaptic overexpression of DVAP-33A induces an increase in the number of boutons and a decrease in their size. Gain-of-function experiments show that the presynaptic dose of DVAP-33A tightly modulates the number of synaptic boutons. Our data also indicate that the presynaptic microtubule architecture is severely compromised in DVAP-33A mutants. We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding.

TitelDrosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
VerfasserPennetta, G., Hiesinger, P.R., Fabian-Fine, R., Meinertzhagen, I.A., and Bellen H.J.
VerlagCell Press
Datum20020718
Kennungdoi: 10.1016/S0896-6273(02)00769-9
Quelle/n
Erschienen inNeuron 35(2): 291-306. Cover article
Spracheeng
ArtText
Rechte© 2002 Cell Press. Published by Elsevier Inc.