Similarities of Drosophila rab GTPases based on expression profiling: completion and analysis of the rab-Gal4 kit.

Jin, E.J., Chan, C.C., Agi, E., Cherry, S., Hanacik, E., Buszczak, M., and Hiesinger, P.R.— 2012

We recently generated rab-Gal4 lines for 25 of 29 predicted Drosophila rab GTPases. These lines provide tools for the expression of reporters, mutant rab variants or other genes, under control of the regulatory elements of individual rab loci. Here, we report the generation and characterization of the remaining four rab-Gal4 lines. Based on the completed 'rab-Gal4 kit' we performed a comparative analysis of the cellular and subcellular expression of all rab GTPases. This analysis includes the cellular expression patterns in characterized neuronal and non-neuronal cells and tissues, the subcellular localization of wild type, constitutively active and dominant negative rab GTPases and colocalization with known intracellular compartment markers. Our comparative analysis identifies all Rab GTPases that are expressed in the same cells and localize to the same intracellular compartments. Remarkably, similarities based on these criteria are typically not predicted by primary sequence homology. Hence, our findings provide an alternative basis to assess potential roles and redundancies based on expression in developing and adult cell types, compartment identity and subcellular localization.

TitelSimilarities of Drosophila rab GTPases based on expression profiling: completion and analysis of the rab-Gal4 kit.
VerfasserJin, E.J., Chan, C.C., Agi, E., Cherry, S., Hanacik, E., Buszczak, M., and Hiesinger, P.R.
VerlagPublic Library of Science
Datum20120723
Kennungdoi: 10.1371/journal.pone.0040912
Quelle/n
Erschienen inPLoS ONE 7(7): e40912
Spracheeng
ArtText
Rechte© 2012 Jin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.