Preparation of developing and adult Drosophila brains and retinae for live imaging.

Williamson, W.R. and Hiesinger, P.R.— 2010

The Drosophila brain and visual system are widely utilized model systems to study neuronal development, function and degeneration. Here we show three preparations of the brain and visual system that cover the range from the developing eye disc-brain complex in the developing pupae to individual eye and brain dissection from adult flies. All protocols are optimized for the live culture of the preparations. However, we also present the conditions for fixed tissue immunohistochemistry where applicable. Finally, we show live imaging conditions for these preparations using conventional and resonant 4D confocal live imaging in a perfusion chamber. Together, these protocols provide a basis for live imaging on different time scales ranging from functional intracellular assays on the scale of minutes to developmental or degenerative processes on the scale of many hours.

TitelPreparation of developing and adult Drosophila brains and retinae for live imaging.
VerfasserWilliamson, W.R. and Hiesinger, P.R.
VerlagMYJoVE Corporation
ThemaNeuroscience, Issue 37, dissection technique, eye disc, brain culture, photoreceptor, confocal microscopy
Datum20100315
Kennungdoi: 10.3791/1936
Quelle/n
Erschienen inJ Vis Exp (37):
Spracheeng
ArtText
Rechte© 2010, Journal of Visualized Experiments